Purification of closed DNA molecules
A scalable method for purifying ceDNA from eukaryotic cells using alkaline lysis and chromatography addresses production challenges, achieving high-purity ceDNA for improved gene therapy safety and efficacy.
KR1020260119657APending Publication Date: 2026-08-03SANOFI SA(FR)
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Patent Information
- Application Number
- KR1020267020533
- Authority / Receiving Office
- KR · KR
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2024-10-25
- Filing Date
- 2024-11-27
- Publication Date
- 2026-08-03
AI Technical Summary
Technical Problem
Current gene therapy methods using viral vectors face limitations such as delivery capacity and immune responses, while closed DNA (ceDNA) offers advantages but is challenging to produce and purify efficiently for large-scale clinical use.
Method used
A method involving alkaline lysis, chemical pre-clarification, and chromatography steps to purify ceDNA from eukaryotic cells, including anion exchange and polishing chromatography, to produce high-purity ceDNA suitable for therapeutic use.
Benefits of technology
The method enables scalable and efficient production of high-purity ceDNA, reducing nucleic acid impurities and host cell proteins, suitable for large-scale manufacturing and improved safety in gene therapy applications.
✦ Generated by Eureka AI based on patent content.
Abstract
The present disclosure provides an efficient and scalable method for purifying closed DNA (ceDNA) for therapeutic use.
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