Integrated digital PCR systems
The integrated digital PCR system addresses the complexity and cost issues of existing systems by consolidating procedures into a compact microfluidic chip and detection instrument, enabling efficient and precise nucleic acid quantification and mutation detection.
Patent Information
- Application Number
- PCT/US2024/047803
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2024-01-03
- Filing Date
- 2024-09-20
- Publication Date
- 2025-03-27
AI Technical Summary
Existing digital PCR systems are complex, costly, and require skilled personnel due to the need for multiple devices for droplet generation, amplification, and fluorescence signal reading, which hinders the speed of target sequence detection.
A compact, cost-effective, and integrated digital PCR system that consolidates all procedures into a microfluidic chip and a detection instrument with a small footprint, featuring a simplified design with a flow induction unit, optical detector, and signal processor for digital PCR analysis.
The system achieves absolute quantification of nucleic acids, allows for specific detection of rare gene mutations, and provides high precision in distinguishing allelic copy number variations, while reducing operational complexity and increasing speed of target sequence detection.