Combined suspension and adherent cell bioreactor system and related methods

The bioreactor system addresses the limitations of current bioreactor technologies by enabling seamless transition between suspension and adherent cell culture modes, improving productivity and product quality, and simplifying scale-up and media exchange.

WO2025071949A1 Publication Date: 2025-04-03CORNING INC
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Patent Information

Application Number
PCT/US2024/046512
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2023-09-29
Filing Date
2024-09-13
Publication Date
2025-04-03

AI Technical Summary

Technical Problem

Current bioreactor technologies are limited to either suspension or adherent cell culture methods, lacking the capability to seamlessly transition between both modes within the same system, which hinders efficient scale-up and product quality in commercial-scale applications, particularly for AAV viral vector production.

Method used

A bioreactor system designed to operate in both suspension and adherent culture modes, featuring a media conditioning vessel and a bioreactor vessel with a perfusion flow circuit and fluid bypass, allowing for easy switching between modes to accommodate different cell culture requirements.

Benefits of technology

The system enables cost and space savings by allowing a single bioreactor to handle both suspension and adherent cultures, improving specific productivity and product quality, simplifying scale-up, and facilitating efficient media exchange, thereby addressing the limitations of current bioreactor technologies.

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Abstract

A cell culture bioreactor system is provided that includes a media conditioning vessel (MCV) having a conditioning inlet into the MCV and a conditioning outlet out of the MCV, and a bioreactor vessel to hold a cell substrate having a surface for adhering cells thereto. The bioreactor vessel includes a bioreactor inlet and a bioreactor outlet. The system also includes perfusion flow circuit fluidly connecting the conditioning outlet to the bioreactor inlet and the bioreactor outlet to the conditioning inlet, and a fluid bypass configured to fluidly connect the conditioning outlet to the conditioning inlet without flowing fluid through the bioreactor vessel. The system is capable of operating both in a suspension culture mode, where the cell culture media flows through the bypass and bypasses the bioreactor vessel, and in an adherent culture mode, where the cell culture media flows through the bioreactor vessel.
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