Nutrient solution composition for increasing establishment rate and promoting growth of moss plant, and method for cultivating moss plant using same

Abstract

The present invention relates to a nutrient solution composition for increasing the establishment rate and promoting growth of a moss plant, and a method for cultivating a moss plant using same. The nutrient solution composition for promoting growth of a moss plant of the present invention enhances the establishment rate of a moss plant by providing a nutrient composition suitable for the growth of a moss plant, and at the same time contains a high concentration of essential growth elements, thereby enabling the production of a moss plant at low cost and with high efficiency.

Description

A nutrient composition for promoting the growth of moss plants and a method for cultivating moss plants using the same for increasing the rate of establishment and growth of moss plants

[0001] The present invention relates to a nutrient composition for promoting the growth of moss plants to increase the rate of establishment and growth of moss plants and a method for cultivating moss plants using the same.

[0002] Mosses are bryophytes, and can be divided into liverworts, which are entirely thallus without distinction between stem and leaves, and mosses, which are upright plants with a distinct stem and leaves. Mosses mainly live in wetlands, and are intermediate plants that transition from aquatic to terrestrial life. Their roots are rhizomes, and they reproduce by alternating generations, either dioecious or monoecious, with asexual and sexual generations. In the wild, they reproduce by fertilization on rainy days.

[0003] There are currently 16,500 known species of moss, two-thirds of which belong to the phylum Lichens, with the remainder being hornworts and liverworts. Moss, with its remarkable ability to survive and expand with just a little water, is considered ideal for terraforming on Mars. Because it lacks a cuticle layer like leaves to filter out impurities, moss can purify more fine dust than trees of the same area.

[0004] Moss has the characteristic of growing better in shade than in light, and because of its unique habitat environment and growth characteristics, it requires a different growth environment than general plants.

[0005] Meanwhile, cultivation methods using existing nutrient solutions containing elements such as minerals necessary for plant growth primarily cover horticultural and food crops. Existing nutrient solutions include Korean Patent Application No. 10-2012-0018742, which describes a method for cultivating functional plants using mineral solutions, and Korean Patent Application No. 10-2022-0020989, which describes a water-soluble nutrient solution manufacturing technique for cultivating ginseng sprouts. However, these solutions have limitations because they do not consider the nutrient requirements for moss growth.

[0006] In addition, existing widely used nutrient solutions include Hyponex and Knop's solution, but these nutrient solutions are not manufactured as nutrient solutions specifically for moss, and when cultivating moss plants, the nutrient solutions are diluted and used, which has the problem of them not being suitable for the growth conditions of moss.

[0007] In particular, moss plants do not have a high demand for nutrients through nutrient solutions, and rather, it is known that when the concentration of nutrients is high, growth rate decreases and physiological disorders occur. Therefore, it is necessary to develop a nutrient solution that is effective for the growth of moss plants by combining minerals that are specific to moss plants with the existing nutrient solution components used for plants.

[0008] In order to solve the above problem, the present invention has a technical significance in providing a cultivation method that increases economic feasibility by efficiently increasing the growth of moss plants through the composition of a nutrient solution suitable for the growth of moss plants and by providing a method for cultivating moss plants using a nutrient solution suitable for the growth of moss plants, thereby producing moss at low cost and with high efficiency.

[0009] In order to achieve the above technical significance, the present invention provides an agent A comprising KNO3, Ca(NO3) 4H2O, Fe-EDTA and Mg(NO3)2; and

[0010] KNO3, MgSO4·7H2O, NH4H2PO4, H3BO3, MnSO4·H2O, ZnSO4·7H2O, CuSO4·5H2O and (NH4)6Mo7O 24 ·Provides a nutrient solution composition for promoting moss plant growth comprising a B agent containing 4H20.

[0011] The nutrient solution composition for promoting the growth of moss plants of the present invention improves the rate of establishment of moss plants through the composition of a nutrient solution suitable for the growth of moss plants, and at the same time contains a high concentration of elements necessary for growth, enabling the production of moss plants at low cost and with high efficiency.

[0012] Figure 1 is a drawing showing the appearance of a nutrient solution composition, which is an example of the present invention, and commercially available nutrient solutions, such as Hyponex and Knop.

[0013] Figure 2 is a drawing showing a method for measuring area ratio and length according to the degree of moss growth according to an experimental example of the present invention.

[0014] Figure 3 is a drawing showing the results of measuring the length of moss cultured according to an embodiment and a comparative example of the present invention.

[0015] FIG. 4 (FIGS. 4a to 4e) are drawings showing the appearance of moss cultured for 30 days on day 0, day 14, and day 30 according to an embodiment of the present invention.

[0016] Figure 5 (Figures 5a to 5e) is a drawing showing the appearance of moss cultured for 30 days on day 0, day 14, and day 30 according to a comparative example of the present invention.

[0017] Figure 6 is a graph comparing the weight, length, and growth rate of moss cultured according to an example and a comparative example of the present invention.

[0018] A composition containing KNO3, Ca(NO3)·4H2O, Fe-EDTA and Mg(NO3)2; and

[0019] KNO3, MgSO4·7H2O, NH4H2PO4, H3BO3, MnSO4·H2O, ZnSO4·7H2O, CuSO4·5H2O and (NH4)6Mo7O 24 ·A nutrient solution composition for promoting moss plant growth comprising a B agent containing 4H20.

[0020] Hereinafter, the present invention will be described in more detail.

[0021] The terms used in this invention have been selected from widely used, common terms, taking into account their functions. However, these terms may vary depending on the intentions of those skilled in the art, precedents, the emergence of new technologies, etc. Therefore, the terms used in this invention should be defined based on their meaning and the overall content of the invention, rather than simply their names.

[0022] When a part of a specification is said to "include" or "have" a component, this does not mean that it excludes other components, but rather that it may include or have other components, unless otherwise specifically stated.

[0023] The term "moss plant" (bryophyte) for cultivation encompasses both sphagnum moss and moss plants other than sphagnum moss, unless otherwise specified herein. Therefore, unless otherwise specified herein, "moss plant" is used to encompass both sphagnum moss and moss plants other than sphagnum moss.

[0024] Throughout this specification, "%" used to indicate the concentration of a particular substance is (wt / wt)% for solid / solid, (wt / vol)% for solid / liquid, and (vol / vol)% for liquid / liquid, unless otherwise stated.

[0025] In the present invention, "sphagnum moss" includes all mosses belonging to the sphagnum genus (Sphagnum L.) of the moss plant family Sphagnum, including Sphagnum palustre L., Sphagnum papillosum Lindb., Sphagnum magellanicum Brid., Sphagnum aongstroemii C. Hartm, Sphagnum microporum Warnst. ex Card, Sphagnum subsecundum Nees ex Sturm, Sphagnum girgensohnii Russow, Sphagnum fuscum (Schimp.) H. Klinggr., Sphagnum fimbriatum Wilson ex Wilson & Hook. f., Sphagnum capillifolium (Ehrh.) Hedw., Examples include, but are not limited to, Sphagnum tenellum Hoffm., Sphagnum cuspidatum Hoffm., Sphagnum recurvum P. Beauv. var. tenue Klinggr., Sphagnum squarrosum Crome, Sphagnum compactum DC., Sphagnum calymmatophyllum Warnest. & Card., and Sphagnum junghuhnianum Dozy & Molk. Subsp. Pseudomolle(Warnest.) H. Suzuki.

[0026] In the present invention, "moss plants other than sphagnum moss" refers to moss plants other than sphagnum moss belonging to the sphagnum genus (Sphagnum L.) of the moss plant family Sphagnum moss. The types of moss plants other than sphagnum moss are not particularly limited. For example, sphagnum moss (Atrichum P. Beauv) such as sphagnum undulatum (Hedw.) P. Beauve; sphagnum moss (Pogonatum P. Beauv) such as sphagnum moss (Pogonatum inflexum (Lindb.) Lac.); sphagnum moss (Polytrichum Hedw.) such as sphagnum commune Hedw.; sphagnum moss (Ceratodon Bird.) such as sphagnum moss (Ceratodon purpureus (Hedw.) Bird.); Dicranum Hedw., including Dicranum japonicum Mitt., Dicranum nipponense Besch, Dicranum scoparium Hedw., and Dicranum polysetum Sw.; Leucobryum Hampe, including Leucobryum juniperoideum (Brid.) C.Mull.; Bryum Hedw., including Bryum argenteum Hedw.; Rhodobryum (Schimp.) Hampe, including Rhodobryum giganteum (schwaegr.) Par.; Plagiomnium T.Kop., such as Plagiomnium acutum (Lindb.) T.Kop.; Trachycystis Lindb., such as Trachycystis microphylla (Dozy et Molk.) Lindb.; Pyrrhobryum dozyanum (Lac.) Manuel) etc.; Bartramia pomiformis Hedw. etc.; Bartramia Hedw.(tamagoke-zoku) etc.; Climacium dendroides(Hedw.)Web.et Mohr, Climacium japonicium Lindb. etc.; Examples include, but are not limited to, the genus Racomitrium Brid., such as Racomitrium ericoides (Web. et Brid) Brid), Racomitrium canescens (Hedw.) Brid.ssp.latifolium), and Racomitrium ericoides; the genus Hypnum Hedw. nom.cons., such as Hypnum plumaeforme Wils.; and the genus Thuidium Bruch et Schimp.in BSG, such as Hypnum Kanedae Sak.

[0027] One aspect of the present invention comprises: A composition comprising KNO3, Ca(NO3)·4H2O, Fe-EDTA and Mg(NO3)2; and

[0028] KNO3, MgSO4·7H2O, NH4H2PO4, H3BO3, MnSO4·H2O, ZnSO4·7H2O, CuSO4·5H2O and (NH4)6Mo7O 24 ·A nutrient solution composition for promoting moss plant growth comprising a B agent containing 4H20.

[0029] The above A agent may contain KNO36 to 16 g / L, Ca(NO3)·4H2O 16 to 26 g / L, Fe-EDTA 1.1 to 1.9 g / L, and Mg(NO3)2 18 to 28 g / L.

[0030] According to one embodiment of the present invention, the A agent may preferably include KNO3 11 g / L, Ca(NO3)·4H2O 21 g / L, Fe-EDTA 1.538 g / L, and Mg(NO3)223 g / L.

[0031] The above B agent is KNO36 to 16 g / L, MgSO4·7H2O 2 to 7 g / L, NH4H2PO4 11.5 to 21.5 g / L, H3BO3 0.01 to 0.15 g / L, MnSO4·H2O 0.076 to 0.086 g / L, ZnSO4·7H2O 0.006 to 0.012 g / L, CuSO4·5H2O 0.001 to 0.007 g / L and (NH4)6Mo7O 24 ·4H20 may contain 0.0001 to 0.002 g / L.

[0032] According to one embodiment of the present invention, the B agent is preferably composed of KNO3 11 g / L, MgSO4·7H2O 5 g / L, NH4H2PO4 16.5 g / L, H3BO3 0.114 g / L, MnSO4·H2O 0.081 g / L, ZnSO4·7H2O 0.009 g / L, CuSO4·5H2O 0.004 g / L and (NH4)6Mo7O 24 ·May contain 0.001 g / L of 4H20.

[0033] The above nutrient composition may be a composition in which the above agent A is diluted in water 100 to 2,000 times and the above agent B is diluted in water 100 to 2,000 times, for example, the above agent A may be diluted in water 1,000 times and the above agent B may be diluted in water 1,000 times, respectively.

[0034] Another aspect of the present invention is a method for cultivating a moss plant by treating the nutrient solution composition to the moss plant.

[0035] The method may include a step of treating the moss plant with the nutrient composition at a concentration of 12.5% ​​to 200% by volume, 12.5% ​​to 100% by volume, 25% to 200% by volume, 25% to 100% by volume, 50% to 200% by volume, or 50% to 100% by volume.

[0036] The above volume % may refer to the concentration of the nutrient composition by diluting the composition in water or concentrating it in water.

[0037] According to one embodiment of the present invention, the method may preferably include a step of treating the moss plant by diluting it in water at a concentration of 50% by volume.

[0038] The above method may further include a step of treating the moss plant with a plant growth hormone.

[0039] The above plant growth hormone may include at least one selected from the group consisting of abscisic acid, cytokinin, gibberellic acid, ethylene, and auxin, but is not limited thereto.

[0040] Hereinafter, the present invention will be described in more detail with reference to the accompanying drawings and examples. However, these examples are intended only to illustrate the present invention, and the scope of the present invention is not limited by these examples.

[0041]

[0042] Manufacturing example. Manufacturing of a nutrient solution composition for promoting moss plant growth.

[0043] Macroelements (N, K, P, Ca, and Mg) necessary for moss plant growth were diluted to match the nutritional requirements of moss, and trace elements (Cl, Fe, B, Mn, Zn, Cu, and Mo) were added in very small amounts suitable for moss growth to prepare a nutrient solution composition having the composition shown in Table 1 below.

[0044]

[0045] Category Composition (g / L) A Article KNO311Ca(NO3)·4H2O21Fe-EDTA (12.5%)1.538Mg(NO3)223B No. KNO311MgSO4·7H2O5NH4H2PO416.5H3BO30.114MnSO4·H2O0.081ZnSO4·7H2O0.009CuSO4·5H2O0.004(NH4)6Mo7O 24 ·4H200.001

[0046]

[0047] <Example>

[0048] A 1 L nutrient solution was prepared by mixing 1 mL of each of the A and B solutions of the nutrient solution compositions prepared according to the above manufacturing example with 998 mL of water, and diluting this to concentration ratios of 200% by volume, 50% by volume, 25% by volume, and 12.5% ​​by volume based on a concentration of 100% by volume, and preparing the examples in Table 2 below.

[0049]

[0050] Example 1 Example 2 Example 3 Example 4 Example 5 Concentration ratio 200% 100% 50% 25% 12.5%

[0051]

[0052] <Comparative Example>

[0053] To compare the growth of moss plants, Knop's solution and Hyponex (N:P:K=20:20:20; Hyponex Japan Co., Ltd., Osaka, Japan), which are widely used as conventional plant growth nutrient solutions, were used. Hyponex was prepared by diluting 1 g, 0.5 g, and 0.25 g in 1 L of water. As a negative control, only water was used and sprayed on moss. Specific comparative examples are shown in Table 3 below.

[0054]

[0055] Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Nutrient Solution Composition Control Knop Hyponex (1 g / L) Hyponex (0.5 g / L) Hyponex (0.25 g / L)

[0056]

[0057] Experimental Example: Comparison of Moss Growth

[0058] To compare the growth of moss, roof red moss (2 g) was sprayed three times a week and grown for 30 days. The growth conditions were temperature 25±3℃, humidity 60±20%, and illumination 22,000 lux (16h / 8h), and the weight (fresh weight, dry weight), length (length), and establishment rate (coverage) of the moss for 30 days were evaluated.

[0059] Specifically, first, the length of the moss was measured, and then a specific length was converted into pixels, and the length (mm) was calculated as the sum of the pixels, and the area was calculated by dividing the moss growth area by the total soil area after spraying each nutrient solution. After calculating the length and area, the measurement values ​​were obtained using the Image J program. After that, each measurement value was calculated as an average value after repeating the phenotype of each moss 5 times, and the results are shown in Table 4 below and FIG. 6 of the present invention.

[0060]

[0061] Fresh weight (g, 30 days) Dry weight (g, 30 days) Length (mm, 30 days) Coverage (%, 14 days) Coverage (%, 30 days) Example 14.66 0.69 9.5 19.62 53.95 Example 25.33 0.75 9.5 23.80 63.30 Example 35.73 0.87 9.62 3.58 67.24 Example 45.66 0.84 8.72 0.43 51.58 Example 55.54 0.80 9.02 1.79 50.72 Comparative example 13.35 0.45 7.42 0.70 48.85 Comparative example 24.64 0.69 7.72 3.24 53.84 Comparative example 35.480.7210.022.4462.15Comparative example 44.510.629.722.0060.00Comparative example 53.730.4610.118.2046.36

[0062]

[0063] As a result of the above experiment, when moss was cultured with Comparative Example 3 in which Hyponex was diluted to 1 g / L, the dry weight was measured to be 0.72 g, and when further diluted to 0.5 g / L (Comparative Example 4) and 0.25 g / L (Comparative Example 5), the weight of the moss decreased further. In comparison, Example 2, which is a nutrient solution composition of the present invention, showed a moss weight increase of 0.03 g (0.75 g) compared to Comparative Example 3, and the 50 volume% concentration (Example 3) diluted to 1 / 2 showed a 20% increase (0.87 g) in the dry weight of the moss. In addition, it was confirmed that the weight increased compared to the Comparative Example in the case of Example 4 diluted to 1 / 4 (25 volume% concentration) and Example 5 diluted to 1 / 8 (12.5 volume% concentration). Additionally, an improved result could be confirmed compared to Comparative Example 2 (Knop liquid).

[0064] The moss area ratio (%) by the rate of establishment (coverage) showed an 8% increase compared to 1 g / L of Hyponex (Comparative Example 3) when diluted by 1 / 2 (50 volume% concentration, Example 3). In addition, an increased result was confirmed compared to the Knop solution (Comparative Example 2).

[0065] In summary of the above experimental results, it was confirmed that the moss-only nutrient solution composition of the present invention has a nutrient solution composition suitable for the growth of moss plants, and is more efficient than existing plant growth nutrient solutions (Knop solution, Hyponex) in moss growth (Figs. 4a to 4e and Figs. 5a to 5e).

[0066] While the present invention has been described in detail through representative examples above, those skilled in the art will understand that various modifications to the above-described embodiments are possible without departing from the scope of the present invention. Therefore, the scope of the present invention should not be limited to the described embodiments, but should be defined not only by the claims described below but also by all changes or modifications derived from the claims and equivalent concepts.

[0067] The present invention has technical significance in that it provides a cultivation method that increases economic feasibility by producing moss at low cost and high efficiency by efficiently increasing the growth of moss plants through the composition of a nutrient solution suitable for the growth of moss plants and by providing a method for cultivating moss plants using a nutrient solution suitable for the growth of moss plants.

Claims

1. A composition containing KNO3, Ca(NO3)·4H2O, Fe-EDTA and Mg(NO3)2; and KNO3, MgSO4·7H2O, NH4H2PO4, H3BO3, MnSO4·H2O, ZnSO4·7H2O, CuSO4·5H2O and (NH4)6Mo7O 24 ·A nutrient solution composition for promoting the growth of moss plants comprising a B agent containing 4H20.

2. In paragraph 1, The above A composition is a nutrient solution for promoting the growth of moss plants, which contains KNO36 to 16 g / L, Ca(NO3)·4H2O 16 to 26 g / L, Fe-EDTA 1.1 to 1.9 g / L, and Mg(NO3)2 18 to 28 g / L.

3. In paragraph 1, The above B agent contains KNO36 to 16 g / L, MgSO4·7H2O 2 to 7 g / L, NH4H2PO4 11.5 to 21.5 g / L, H3BO3 0.01 to 0.15 g / L, MnSO4·H2O 0.076 to 0.086 g / L, ZnSO4·7H2O 0.006 to 0.012 g / L, CuSO4·5H2O 0.001 to 0.007 g / L and (NH4)6Mo7O 24 ·A nutrient solution composition for promoting the growth of moss plants, comprising 0.0001 to 0.002 g / L of 4H20.

4. In paragraph 1, A nutrient solution composition for promoting the growth of moss plants, wherein the nutrient solution composition is prepared by diluting the A agent in water 100 to 2,000 times and the B agent in water 100 to 2,000 times, respectively.

5. A method for cultivating moss plants by treating the nutrient composition of Article 1 to the moss plants.

6. In paragraph 5, A method for cultivating a moss plant, the method comprising the step of treating the moss plant with the nutrient composition at a concentration of 12.5% ​​to 200% by volume.

7. In paragraph 5, A method for cultivating a moss plant, wherein the method further includes a step of treating the moss plant with a plant growth hormone.

8. In paragraph 7, A method for cultivating a moss plant, wherein the plant growth hormone comprises at least one selected from the group consisting of abscisic acid, cytokinin, gibberellic acid, ethylene, and auxin.

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