Method for detecting single-stranded nucleic acid

By employing multiple fluorescent labeling dyes with varying affinities for single-stranded and double-stranded nucleic acids and adjusting mixing order, the method enhances detection sensitivity of single-stranded nucleic acids in the presence of double-stranded nucleic acids, addressing the limitations of existing techniques.

WO2025203643A1 Publication Date: 2025-10-02HITACHI HIGH TECH CORP
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Patent Information

Application Number
PCT/JP2024/013275
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Filing Date
2024-03-29
Publication Date
2025-10-02

AI Technical Summary

Technical Problem

Existing methods for detecting single-stranded nucleic acids in the presence of double-stranded nucleic acids, such as in liquid biopsies, face challenges in sensitivity and background noise, making it difficult to accurately detect single-stranded nucleic acids.

Method used

The method involves using multiple fluorescent labeling dyes that preferentially adsorb to nucleic acids, specifically combining dyes with different affinities for single-stranded and double-stranded nucleic acids, and adjusting the order of dye mixing to enhance detection sensitivity.

Benefits of technology

This approach significantly improves the detection sensitivity of single-stranded nucleic acids by 1.3 to 4.1 times compared to using a single dye, reducing background noise and enhancing signal intensity.

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Abstract

This method for detecting single-stranded nucleic acids in a sample containing single-stranded nucleic acids and double-stranded nucleic acids comprises: labeling the nucleic acids using multiple labeling reagents adsorbed by the nucleic acids; and performing electrophoresis of a sample containing the labeled nucleic acids to measure the signal intensity.
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