Streptococcus mutans circular RNA circcsbd, use thereof, and overexpression strain thereof, construction method therefor, and use thereof

By discovering and validating the closed circular structure of the circular RNA circcsbD in Streptococcus mutans and constructing circcsbD overexpressing strains, the problem of caries biofilm formation was solved, enabling effective prediction and prevention of caries.

WO2025227846A1 Publication Date: 2025-11-06SICHUAN UNIV
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Patent Information

Application Number
PCT/CN2025/072468
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-04-30
Filing Date
2025-01-15
Publication Date
2025-11-06

AI Technical Summary

Technical Problem

The lack of research and application of circular RNA from Streptococcus mutans in current technologies makes it difficult to effectively predict and control biofilm formation in dental caries, leading to difficulties in the prevention and treatment of dental caries.

Method used

The existence and closed circular structure of endogenous circular RNA circcsbD in Streptococcus mutans were discovered and verified. A circcsbD overexpression strain was designed and constructed to inhibit extracellular polysaccharide metabolism in biomembranes and reduce cariogenicity by overexpressing circcsbD.

Benefits of technology

The circcsbD overexpression strain can significantly inhibit the formation of biofilm and the synthesis of extracellular polysaccharides in Streptococcus mutans, reduce the risk of dental caries, provide new biomarkers and therapeutic targets for dental caries, and realize the auxiliary prediction and prevention of dental caries.

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Abstract

A Streptococcus mutans circular RNA circcsbD and use thereof are provided. The nucleotide sequence of circcsbD is set forth in SEQ ID NO: 1. circcsbD can be used as a biomarker for caries. Use of circcsbD in the preparation of a formulation for preventing or treating oral caries is provided. The present invention further relates to a primer and kit for detecting circcsbD, and a strain of Streptococcus mutans overexpressing circcsbD, a construction method therefor, and use of the overexpression strain. The endogenous circular RNA circcsbD is found in the prokaryote Streptococcus mutans for the first time. The expression level of circcsbD can be used for predicting caries. circcsbD can be used as a caries-related biomarker and a treatment target. The strain overexpressing circcsbD has the effects of inhibiting extracellular polysaccharide metabolism on the biofilm of Streptococcus mutans and reducing the cariogenic capacity of Streptococcus mutans.
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