Method for producing multi-tissue lung organoid surrounded by fibroblasts, and use thereof
By structurally wrapping lung organoids with fibroblasts, the method addresses the limitations of existing organoid technologies, achieving improved physiological accuracy and reproducibility for disease modeling and drug response analysis.
Patent Information
- Application Number
- PCT/KR2025/007190
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2025-05-23
- Filing Date
- 2025-05-27
- Publication Date
- 2025-12-04
AI Technical Summary
Existing organoid technologies fail to mimic the interactions between epithelial cells and various non-epithelial cells, such as fibroblasts, such as fibroblasts, vascular endothelial cells, and immune cells, and fibroblasts, such as fibroblasts, vascular endothelial cells, immune cells, etc.) in actual human tissues, leading to gaps in tissue-specific microenvironments and drug response reproducibility.
A method for producing multi-tissue lung organoids by isolating epithelial cells and fibroblasts from lung tissue, culturing them separately, mixing their suspensions, centrifuging to attach fibroblasts to the organoids, and aggregating them to form a structure similar to the biological arrangement in lung tissue, with fibroblasts surrounding the organoids.
The method produces organoids with enhanced physiological accuracy and reproducibility, supporting organoid culture stability and mimicking the biological microenvironment, making them suitable for disease modeling and drug response analysis.