Acyltransferases TRIZ Cases

Specific amino acid substitutions improve transglutaminase heat resistance, oxidation resistance, and reactivity despite reduced temperature stability.

Using the native rice NAT gene, this case shows how overexpression boosts blast resistance across varieties despite genetic background limits.

Engineered acyltransferase, glucosyltransferase, and malonyltransferase pathways enable stable natural blue anthocyanin production in eukaryotic cells.

Increasing 3-hydroxyhexanoate content in microbially produced PHA improves melt fluidity and processability while retaining biodegradability.

KASII gene editing raises palmitic acid in peanut seed oil to limit oil separation and reduce stabilizer use in natural peanut butter.

Specific amino acid substitutions help transglutaminase retain strong reactivity at 50°C or higher for heated industrial cross-linking.

A structured mushroom solid composition uses proteins or polysaccharides to deliver meat-like texture while retaining mushroom health benefits.

Microbial biosynthesis of hydroxycarboxylic acid block copolymers improves PHA extensibility, flexibility, and degradability.

LbZAT1, LbZAT2, and LbZAT3 catalyze fatty-acid esterification of free carotenoids to improve stability and lipid solubility.

Directed-evolution pro-peptide variants limit solution self-crosslinking while preserving transglutaminase activity and yield.

Targeted edits to DGAT1 N-terminal motifs raise lipid production while limiting the genetic complexity of TAG engineering.

Genetically modified Methylobacillus suppresses lysis-related pathways to preserve high cell density and extend metabolic activity during methanol fermentation.

This case uses SMURF1 template structures and αH10 dynamics to screen inhibitors when HECT E3-ligases lack active-site pockets.

This case uses MinC, MinD, and optional MinE expression changes to enlarge cells and PHA particles for aqueous separation.

This case uses recombinant microorganisms and engineered AAT enzymes to convert acryloyl-CoA and butanol into n-butylacrylate.

Biosynthesized poly(3-hydroxypropionate) improves polylactide toughness and flexibility.

Polyketoacyl-CoA thiolases catalyze iterative non-decarboxylative Claisen condensation to elongate polyketide chains using acetyl-CoA extender units.

Human Factor XIII normalization control corrects assay signal variations from reagent and matrix effects, improving HIV diagnostic reliability.

Novel DGAT enzymes accumulate triacylglycerols to increase total fatty acid content in transgenic oilseeds.

Replacing chemical synthesis with acyltransferase enzymes reduces energy consumption and purification complexity while maintaining high productivity.

Microorganisms recycle acetate byproducts into acetyl-CoA, resolving pH disruption and boosting L-methionine production efficiency.

Expanding enzyme substrate scope enables synthesis of non-canonical lipoproteins with distinct assembly pathways, overcoming narrow lipid repertoire limits.

Engineered prenyltransferase variants overcome enzyme instability at high reaction temperatures, enabling efficient cannabinoid production.

Replacing L-aspartic acid with L-valine at position 5 of citrate synthase overcomes biosynthesis efficiency limits to boost L-amino acid yields.

Engineered Yarrowia lipolytica strains overcome low yield bottlenecks by shifting metabolic flux from fatty acids to high-titer Type III polyketide production.

Deleting fadB and fadJ genes in recombinant Escherichia coli prevents random mixtures, enabling precise control over polyhydroxyalkanoate polymer composition.

Engineered microbial hosts convert acetyl-CoA to pimelic acid, resolving the economic bottleneck in sustainable Nylon 7 synthesis.

Engineered alcohol acyl transferase converts tertiary alcohols into esters, overcoming low efficiency of conventional enzymes.

Reduced citrate synthase activity redirects acetyl-CoA flux to boost citramalate yield while minimizing acetate byproducts.

Mutated 23S rRNAs enhance ribosomal peptidyl transferase activity to incorporate beta-amino acids, improving therapeutic physiological lifetime.

Specific amino acid deletions and substitutions in DGAT1 enzymes resolve contradictions between seed oil content and enzyme stability.

Engineered microorganism with deleted competing genes enables continuous ectoine production in low-salt conditions, eliminating equipment corrosion.

Enzymatic conversion of 3-methylcrotonyl-CoA into 3-hydroxy-3-methylbutyric acid using thioesterases and CoA-transferases.