Bacteria TRIZ Cases

Solid state fermented Lactobacillus plantarum strains maintain viability during feed pelleting to reduce mortality rates and stimulate immune responses.

Enhancing glycerol kinase activity in PHA-producing microorganisms to increase polymer molecular weight.

Human monoclonal antibodies bind specifically to the O8E antigen on tumor cells, addressing ineffective treatments for advanced breast and ovarian cancers.

Surface-expressed carbohydrate binding modules anchor host cells to cellulose matrices, resolving cell toxicity bottlenecks during cadaverine production.

Adjusting fermentation broth to pH 8-11 before drying prevents loss of heavy metal binding capacity, enabling effective gastrointestinal removal.

Mutant chorismate-pyruvate lyase resists product inhibition by 4-hydroxybenzoic acid, enabling high-yield biological production from renewable glucose.

Engineered Escherichia coli converts D-lactic acid to L-lactic acid using enhanced lactate dehydrogenase and oxidoreductase enzymes.

A cAMP receptor protein variant with specific amino acid substitutions increases sugar consumption rates in microorganisms.

Replacing expensive yeast extract with defined amino acids reduces medium costs while maintaining high carotenoid productivity in Paracoccus fermentation.

Cultivating microalgae with 475 nm radiation reduces chlorophyll content below 20 ppm while increasing carotenoid levels to 5000 ppm.

Fermenting microorganisms using lactoserum as a carbon source reduces environmental footprint while recycling dairy waste into nutritionally equivalent casein.

Lactobacillus paracasei GKS6 composition increases Cisd2 gene expression, reducing mitochondrial damage and delaying aging conditions.

A bacterial fermentation method using unprecipitated batch and feed media with specific salt ratios and chelating agents.

Strain V3 suppresses colorectal tumors by resisting stomach acid inactivation while maintaining anti-inflammatory metabolite activity.

A Pseudomonas bacterium degrades linear alkylbenzene sulfonates and removes nitrogen from rural domestic sewage.

Adding water-soluble polymers to fermentation media mediates L-amino acid precipitation, resolving the trade-off between high productivity and crystal purity.

Glycosylated fimbriae on the novel L. casei strain resolve low adhesion heterogeneity by anchoring to epithelial cells.

Mutant glucose isomerase enzymes resist xylose inhibition and maintain high conversion rates in crude lignocellulosic hydrolysates.

Engineered E. coli expresses specific transferases to boost lacto-N-neotetraose yield from 0.91 g/L to 12.14 g/L in fermentation.

Dynamic aeration and temperature control in recombinant Bacillus subtilis fermentation boost psicose 3-epimerase activity to 4783 U/mL.

Modifying microorganism L-cysteine biosynthesis enzymes to produce vanillin and vanillic acid from carbon sources.

Alcohol acyltransferase catalyzes the reaction of 3-hydroxyisobutyryl-CoA with alcohols to produce 3-hydroxyisobutyric acid esters.

Isolating a novel enzyme from Palaeococcus helgesonii expands the available source diversity for PCR applications beyond traditional hyperthermophiles.

Microbial induction of iron-manganese oxides transforms mobile arsenic into residual forms, avoiding secondary pollution from physical-chemical treatments.

Cross-linked polyvinyl alcohol hydrogel immobilizes microorganisms to reduce ammonia by 95% and eliminate brine generation.

A B. licheniformis alpha-amylase variant replaces the N-terminal valine with DGL residues to enhance catalytic activity.

Increasing kpsS and kfiA-D gene expression while reducing yhbJ function in Escherichia resolves low productivity in non-animal heparin production.

Engineered cyanobacteria convert carbon dioxide and water into methyl laurate, bypassing rainforest destruction and gas-liquid mass transfer bottlenecks.

Optimized culture medium with balanced carbon and nitrogen sources maximizes high molecular weight dextran yield using Weissella cibaria.

Processed dairy probiotics trigger DNA mutations in mice, revealing the root cause of multiple autoimmune diseases and enabling early dietary intervention.

Engineering specific gene expressions in E. coli resolves low yield bottlenecks, achieving higher alanine production rates.

Solid-state fermentation with cellulolytic bacteria reduces alpha-galactosides and non-starch polysaccharides, enabling higher protein inclusion in salmon feed.

Surface modification of microbial cells with charged polyelectrolytes creates spatially defined aggregates through electrostatic interaction.

Phosphoketolase converts glucose to acetyl-CoA avoiding carbon atom loss while maintaining energy balance for metabolite production.

Isolated Lactobacillus delbrueckii subsp. lactis LDL557 strain reduces joint inflammation and cartilage damage.

An aerobic microorganism consumes trace oxygen to maintain microaerobic conditions, eliminating the need for costly gas pre-treatment systems.

Combining sweeteners and triglyceride oils in fermentation reduces de novo synthesis, raising productivity to 2.7 g/L/h.

Natural acidification during cultivation removes external pH adjusters, preventing capsular polysaccharide modification and harmful substance generation.

A trimeric fusion protein combines a collagen-like domain with an Fc region to increase binding avidity.

Fusion proteins combine antigen binding with immunogenic mediators to overcome production difficulties and accelerate personalized cancer vaccine development.

Modular gene cluster engineering resolves manufacturing complexity by enabling targeted biosynthesis of sanglifehrin analogues with reduced toxicity.

Bifidobacterium animalis subsp. lactis BLa36 colonizes the gut to increase fecal moisture and transit rates, resolving constipation without drug side effects.

Disrupting the acetyl-CoA hydrolase gene reduces acetic acid accumulation, thereby increasing L-amino acid yields during fermentation.

Cloned vitamin D3 hydroxylase gene from Pseudonocardia autotrophica resolves low yield and side reactions in conventional synthesis.

A DNA construct links oligonucleotides to fluorescent dyes and quenching agents for reversible cell surface protein labeling.

Bacillus coagulans MTCC 5856 spores survive brewing and aeration stress, resolving the contradiction between processing efficiency and spore viability.

Combining multiple long chain fatty acids with fats and oils resolves low utilization efficiency, enabling industrial-scale polyhydroxyalkanoate production.

Knocking out degradation genes and removing feedback inhibition enables high-yield citicoline fermentation, reducing pollution compared to chemical synthesis.