Bacteria TRIZ Cases

Inactivating attachment genes releases polysaccharides as free slime, resolving nutrient uptake and purification bottlenecks.

Protease cleavage releases captured exosomes from functionalized biopolymer beads, preserving therapeutic activity and preventing aggregation during isolation.

A lyophilization reagent combines wheat peptone with tryptone soy broth and sucrose to protect bacterial cells during freeze-drying.

Mutating Spirulina to a long linear shape allows simple sieve filtration, reducing biomass loss and ammonia sensitivity during harvest.

Lactobacillus reuteri converts 1,2-propanediol to calcium propionate through alcohol dehydrogenase inactivation.

Synergistic stabilizer mixtures of inulin, maltodextrin, and pea fibre maintain high viable lactic acid bacteria counts through extended shelf life.

A deacidified fruit matrix preserves live Lactobacillus viability through selective organic acid removal.

Substituting glycine at position 77 of the NCgl2522 protein increases putrescine and arginine yield, reducing reliance on petrochemical processes.

A Thermococcus onnurineus NA1 mutant overexpresses F420-reducing hydrogenase to produce hydrogen from formate.

Integrating T-DNA into the Agrobacterium chromosome eliminates vector backbone sequences and stabilizes single-copy transgenes.

Knocking out endogenous gapA gene in Corynebacterium prevents substrate competition, allowing introduced gapN to maximize NADPH production and L-lysine yield.

Lactobacillus Plantarum strain GMNL-662 promotes bone regrowth by increasing osteogenic gene expression, avoiding side effects of bisphosphonates.

Expressing triterpene methyltransferase in bacteria overcomes slow algal growth to produce renewable biofuels at scale.

Replacing expensive nucleoside triphosphates with nucleoside monophosphates and exogenous phosphate lowers reagent costs while sustaining high protein yields.

A fibril cellulose hydrogel matrix supports embedded microbial growth while preventing sedimentation and enabling homogeneous cell division.

A soil-based rhizosphere flow-through system extracts and concentrates microorganisms to break down organic contaminants in water.

An integrated process merges feedstock preparation with gas fermentation to convert carbon sources into valuable fermentation products.

Solid culture medium with chromogenic substrates discriminates VanA/VanB and VanC resistance groups, eliminating false results from low selectivity.

Linear prediction of microbiota mixes corrects errors via interaction models, reducing sequencing time while maintaining diversity.

A recombinant microorganism converts anthranilic acid to methyl anthranilate via enzyme catalysis.

Limosilactobacillus reuteri GL-104 and Lacticaseibacillus rhamnosus F-1 strains modulate immune cell differentiation.

An oil phase dissolves methane and transfers it to aqueous droplets, eliminating mechanical agitation that damages cells during high-density fermentation.

Engineered recombinant bacteria transform fatty acids into 3-hydroxypropionic acid, reducing raw material costs and improving conversion rates.

Segmenting transformation and characterization stages in attenuated Salmonella strains enables rapid, robust manufacturing of patient-specific DNA vaccines.

Engineered microbes convert fumarate into adipate using plant-derived enzymes, eliminating excessive oxidizing agents.

Attenuating lactate dehydrogenase and methylglyoxal synthase genes redirects carbon flux to increase glycolic acid titer while reducing by-product formation.

A recombinant bacterial host cell system maintains heterologous polypeptide expression without external inducer supplementation.

Conditional obligate anaerobes accumulate in hypoxic tumor regions via hypoxia-responsive gene cassettes, eliminating toxicity to normal tissues.

Specific amino acid substitutions in Bacillus pumilus proteases enhance catalytic activity and storage stability.

Citric acid preserves nitrate reductase activity during freezing, maintaining color formation capability in meat products.

A generic inert Salmonella S9H bio-vector displays specific antigen factors on its surface to enable precise agglutination reactions.

Silicic acid protects oxidation-sensitive substances during drying, preventing degradation while maintaining product stability.

Engineered mutant Ldc2 polypeptides catalyze lysine decarboxylation to produce biobased cadaverine in recombinant host cells.

Recombinant bacteria use formate oxidation for energy to drive CO2 fixation, achieving autotrophic growth without organic carbon sources.

Culturing pH-resistant coryneform bacteria overcomes low yield bottlenecks in conventional fermentation by leveraging enhanced lysine decarboxylase activity.

An acetate-inducible promoter weakens target gene expression in recombinant coryneform microorganisms to boost L-amino acid production.

A co-culture bioassay identifies prebiotics that enhance intestinal barrier integrity and modulate immune responses.

Pseudomonas aeruginosa GDUTAN1 degrades monomethylamine through microbial metabolic processes.

A mutant prephenate dehydrogenase desensitized to feedback inhibition boosts L-tyrosine yields in Escherichia cultures.

Disrupting CODH1 and CODH2 genes in carboxydotrophic bacteria redirects carbon flux from acetate byproducts to increase ethanol and 2,3-butanediol yields.

Lactobacillus paracasei strain promotes polyamine production to overcome declining synthesis ability with aging.

A mutant microorganism introduces a modified malate dehydrogenase enzyme to boost succinic acid yield.

Lactobacillus mucosae strain CNCM I-4429 restores intestinal barrier integrity by decreasing paracellular permeability induced by pro-inflammatory cytokines.

Engineered bacteria express galactokinase without the Spot 42 binding region to enable direct galactose assimilation.

Engineered microorganisms produce homogeneous fatty acid derivatives directly from renewable carbon sources via modified metabolic pathways.

Dynamic glmS ribozyme feedback controls metabolic flux in Bacillus subtilis, preventing toxic intermediate accumulation and boosting yields.

Deleting catabolic pathways in a genetically engineered bacterium increases sialyllactose yield to 3 g/L while lowering production costs.