Bacteria TRIZ Cases

Enzymatically releasing nutrients from solid tablets prevents toxic metabolite accumulation during high-density cell culture.

Mutant Corynebacterium glutamicum strains resist feedback inhibition from L-isoleucine derivatives, increasing production yields by 13 times.

Optimized Streptomyces ActE secretome digests lignocellulosic materials, resolving low degradation efficiency of conventional species.

Pediococcus strains overcome butanol toxicity to host cells, enabling high-titer bioproduction of 1-butanol, 2-butanol, and isobutanol via genetic engineering.

Lachnospiraceae bacteria produce short-chain fatty acids that attenuate radiation-induced hematopoietic and gastrointestinal injuries.

Engineered Escherichia coli replaces harsh chemical synthesis with biological fermentation, achieving 10 g/L sarcosine titer via metabolic pathway optimization.

A carbonyl reductase variant with an F85M mutation enables asymmetric reduction of 4-chloroacetoacetate.

A genetically modified Clostridium acetobutylicum strain converts industrial glycerine to 1,3-propanediol using a vitamin B12-independent enzyme.

Deleting lpxL and lpxM genes in Vibrio sp. reduces endotoxin contamination, eliminating costly purification steps and preserving product yield.

Surface-displayed binding agents enable engineered microorganisms to selectively bind and eliminate gut pathogens while preserving commensal microbiota.

The CDS2657 gene suppresses hydroxyl radical production to enhance hydrogen peroxide resistance in microorganisms.

Synergistic Bacillus and Lactobacillus strains degrade immunogenic gluten peptides, preventing adverse reactions in individuals with celiac disease.

CECT 9174 strain adheres to dental surfaces and secretes bacteriocins to inhibit S. mutans, preserving oral microbiome balance.

Modifying the mutS mismatch repair pathway via a nirA promoter accelerates adaptive evolution, yielding strains with higher salt tolerance and sucrose yields.

Fed-batch rhamnolipid fermentation extends the stationary phase through controlled nitrogen supplementation to boost product concentration.

Autochthonous microflora preparations enriched with bacteriophages restore intestinal balance through targeted pathogen elimination and bacterial colonization.

Engineered microorganisms expressing specific 4-hydroxybenzoate hydroxylases catalyze the hydroxylation of 4-aminobenzoic acid to produce 3-hydroxy-4-aminobenzoic acid.

Oral probiotic composition modulates immune response to reduce dyspnea frequency and intensity, addressing limitations of direct respiratory interventions.

Fermentation using recombinant polyketide synthases produces dicarboxylic acids, reducing petroleum dependence for nylon and polyester manufacturing.

Deleting the bct operon in Sphingomonas ZD001 eliminates yellow pigments, reducing solvent consumption and simplifying gellan gum purification.

A crop management system calculates nitrogen and water deficits to generate application schedules.

Basic neutralizing agents in culture media maintain pH levels to increase microbial cell concentration and fermentative activity.

Engineering mesophilic bacteria with pH-insensitive enzymes eliminates costly pH adjustments, enabling continuous GABA production from methanol.

Gene amplification and promoter replacement in Corynebacteria elevate key enzyme activities to resolve low productivity bottlenecks.

Targeted amino acid substitutions in domain C of protein A improve alkali stability, maintaining antibody-binding capacity during affinity support regeneration.

Selection method identifies Lactobacillus plantarum strains with high ethanol tolerance using D-cycloserine resistance as a predictive marker.

Wild-type Bacillus endophyticus produces fluorescent material that detects heavy metals via fluorescence inhibition, avoiding GMO regulatory complexity.

Composite plant fibers protect probiotics from acid hydrolysis while serving as substrates to inhibit pathogenic bacteria and produce short-chain fatty acids.

A recombinant gram-negative bacterial cell strain expressing DsbC disulfide bond catalysts to enhance protein folding efficiency.

Introducing xylose isomerase and xylulokinase enables Corynebacterium to metabolize xylose for L-lysine production.

Dimorphic colony selection isolates specific mycobacteria morphologies to improve vaccine reliability and host adaptability.

Pre-activating the glycosylating enzyme system with demethylated colchicinoids overcomes intermediate accumulation and low productivity in biotransformation.

A genetically engineered biological indicator uses a reporter gene and repressor system to detect sterilization efficacy through enzyme activity.

Isolating Rhodococcus ruber cell wall components treats burns and scalds by accelerating tissue regeneration while minimizing scarring.

Knocking out hydroxylase genes prevents product oxidation, enabling high-yield Danshensu fermentation.

Integrating a second prsA gene expression cassette at the amyL locus in Bacillus licheniformis boosts protein production yields.