Carbon-Nitrogen Lyases TRIZ Cases
- Microbial GAA Production via Enzyme Pathway Engineering
Overexpressing amidinotransferase and aminotransferase in engineered microbes increases guanidinoacetic acid yield while reducing metabolic burden.
- Microbial Caffeic Acid Synthesis via Soluble Enzyme Pathway
Engineered microbes synthesize caffeic acid using soluble TAL and 4HPA3H enzymes to bypass unstable membrane-bound plant proteins.
- Microorganism Metabolic Engineering for Vanillin Production
Modifying purH and serA genes in microorganisms overcomes low bio-production yields by optimizing metabolic flux for efficient vanillin accumulation.
- Branched-Chain Ketoacid Dehydrogenase for Malonyl-CoA Synthesis
Recombinant cell strains expressing branched-chain alpha-ketoacid dehydrogenase complexes catalyze malonyl-CoA formation from oxaloacetate.
- Malonyl-CoA Provision in Coryneform Bacteria
Targeted fasB and gltA modifications boost malonyl-CoA availability without inhibiting cell growth.
- Metabolically Engineered Microorganisms for Resveratrol Production
Engineered microorganisms synthesize resveratrol via the phenylpropanoid pathway, replacing low-yield plant extraction with high-efficiency fermentation.
- Frambinone Production via Genetically Modified Fungal Microorganism
Genetically modified fungi produce frambinone from tyrosine by channeling metabolic pathways to prevent unwanted breakdown and reduce by-product formation.
- Enzyme-Catalyzed Tryptamine Derivative Synthesis
Enzymes catalyze substitution reactions on indole acceptors to produce novel tryptamine derivatives, reducing product inhibition in microbial host cells.
- Genome-wide Mutations for Enhanced Lysine Production in E. coli
Segmenting genome-wide mutagenesis into targeted modules resolves the contradiction between high lysine yield and genetic engineering complexity.
- Amino Resin Enzyme Immobilization via Polymer Cross-Linking
Polymer-modified amino resin carriers stabilize enzymes through network cross-linking, enabling efficient recycling in biocatalytic reactions.