Carbon-Nitrogen Lyases TRIZ Cases

Overexpressing amidinotransferase and aminotransferase in engineered microbes increases guanidinoacetic acid yield while reducing metabolic burden.

Engineered microbes synthesize caffeic acid using soluble TAL and 4HPA3H enzymes to bypass unstable membrane-bound plant proteins.

Modifying purH and serA genes in microorganisms overcomes low bio-production yields by optimizing metabolic flux for efficient vanillin accumulation.

Recombinant cell strains expressing branched-chain alpha-ketoacid dehydrogenase complexes catalyze malonyl-CoA formation from oxaloacetate.

Targeted fasB and gltA modifications boost malonyl-CoA availability without inhibiting cell growth.

Engineered microorganisms synthesize resveratrol via the phenylpropanoid pathway, replacing low-yield plant extraction with high-efficiency fermentation.

Genetically modified fungi produce frambinone from tyrosine by channeling metabolic pathways to prevent unwanted breakdown and reduce by-product formation.

Enzymes catalyze substitution reactions on indole acceptors to produce novel tryptamine derivatives, reducing product inhibition in microbial host cells.

Segmenting genome-wide mutagenesis into targeted modules resolves the contradiction between high lysine yield and genetic engineering complexity.

Polymer-modified amino resin carriers stabilize enzymes through network cross-linking, enabling efficient recycling in biocatalytic reactions.

Replacing halophilic hosts with recombinant Corynebacterium glutamicum eliminates endotoxin removal steps while maintaining high ectoine concentration.

An enzymatic pathway incorporates formaldehyde into biomass via a novel serine cycle variant.

Engineered tyrosine ammonia-lyase polypeptides resolve proteolysis sensitivity and acidic pH intolerance while maintaining high catalytic efficiency.

Engineered yeast cells resolve phage contamination risks by transferring production pathways from bacterial systems.

A recombinant microorganism strain produces cinnamaldehyde using integrated pal, 4cl, and ccr genes.

Engineered strain uses ilvA, argP, and fepA modifications to overcome metabolic pathway complexity limits in valine production.

Engineered microorganisms produce guanidinoacetic acid via carbamoylphosphate synthase and amidinotransferase enzymes, addressing low production efficiency.

Yeast factories express strictosidine-beta-glucosidase to convert strictosidine into aglycone, replacing costly chemical synthesis.

Segmented microbial polycultures boost flavanone titers by resolving trade-offs in precursor requirements.

Engineered recombinant Escherichia coli strain produces chlorogenic acid via metabolic pathway construction.

Adenylosuccinate modulators regulate IMP to AMP conversion pathways, alleviating core autism symptoms and improving neurodevelopmental trajectories.

Engineered microorganisms produce guanidinoacetic acid by expressing heterologous genes that resolve glycine availability bottlenecks in fermentation.

Deleting degradation genes speE and speG in mutant microorganisms prevents cadaverine loss during aerobic fermentation.

Oral administration of this probiotic degrades phenylalanine before absorption, bypassing expensive enzyme therapies and dietary restrictions.

A recombinant Escherichia coli single-cell factory synthesizes alpha-aminobutyric acid using optimized enzyme expression and cofactor regeneration.

Engineered microbes secrete anti-cancer drugs locally to reduce systemic toxicity.

Site-specific amino acid substitutions in engineered phenylalanine ammonia-lyase polypeptides extend half-life and stability against digestive degradation.

Attenuating yafV and ilvA expression resolves the trade-off between threonine productivity and strain growth properties.

Recombinant polypeptides with high tyrosine specificity catalyze hydroxycinnamic acid synthesis.

Whole-cell biocatalysis using engineered E. coli replaces harsh chemical processes, achieving 98% conversion with minimal intermediate buildup.

Inhibiting nucleotide biosynthesis enzymes decouples cell growth from production, increasing mevalonate yield by 41% and GFP production by 2.2-fold.

A prokaryotic microbial cell produces raspberry ketone through aerobic fermentation.

Engineered tyrosine ammonia lyase polypeptides convert tyrosine to p-coumaric acid while resisting proteolysis and acidic pH.

Heterologous enzyme expression in recombinant Escherichia coli boosts rosmarinic acid yield to 511.2 mg/L, replacing inefficient plant extraction.

Quantum dot-bound enzymes channel substrates through a four-enzyme cascade to produce 1,3-diaminopropane at room temperature.