Fermentation TRIZ Cases

Replacing petrochemical feedstocks, recombinant microbes produce phenol from glucose using segmented enzymatic pathways.

Expressing isolated (+)-cedrol synthase in recombinant host cells replaces unsustainable conifer extraction, enabling scalable industrial terpene synthesis.

A modified ornithine decarboxylase protein increases putrescine conversion activity through targeted amino acid mutations.

Replacing urea with calcium carboxylic acids and chaA-expressing bacteria eliminates malodor while converting gaseous CO2 into stable solid calcium carbonate.

Genetic transformation introduces herbicide and disease resistance into parental lines, accelerating trait integration while maintaining agronomic stability.

Modifying the 5'UTR of the ppc gene regulates metabolic flux to produce PHA copolymers with reduced brittleness and improved processability.

A two-step procedure uses a thermostable endopeptidase to unfold substrate proteins before adding a secondary protease for deep hydrolysis.

Modified bacteria enhance sucE1 and mdh gene expression to produce organic acids under aerobic conditions.

A synthetic double-stranded DNA template enables direct in vitro transcription of mRNA and functional proteins.

Amino acid mutations in isoprene synthase boost monomer productivity while maintaining enzymatic stability for cost-effective production.

Modifying the cj1 promoter to cj2.2 boosts transcription activity, resolving insufficient yield in conventional E. coli systems.

CV035789 corn variety applies preliminary self-pollination and segmentation to resolve genetic stability versus predictability trade-offs in hybrid breeding.

Segmented breeding stabilizes homozygous parent lines to resolve the contradiction between genetic diversity and performance predictability in tomato hybrids.

Microbacterium sp. glycosidase MT619 hydrolyzes the C-6 sugar of major ginsenosides to produce minor ginsenosides.

Segmented breeding stabilizes inbred lines before crossing, resolving the contradiction between genetic uniformity and adaptability in hybrid corn development.

Genetic modification of CHO cells eliminates costly enzymatic trimming steps while ensuring macrophage targeting for Gaucher's disease treatments.

A DNA cassette positions a promoter sequence to drive transcription directly into a DnaA-binding replication origin within a plasmid construct.

Adjusting crystallization pH above 7.0 reduces nitrogen content below 800 ppm in steviol glycoside compositions derived from fermentation broths.

Inactivated host cell genes reduce methanol utilization, eliminating hydrogen peroxide generation and safety risks during high-density culture.

Engineered tRNASec enables selenocysteine incorporation into polypeptides without requiring a SECIS element in the mRNA sequence.

Recombinant Escherichia coli expressing novel pap-like genes overcomes low productivity limits to achieve 1.8 g/L aniline derivative yields.

Tomato variety N 6416 resolves genetic non-uniformity by establishing homozygous parental lines before crossing to ensure stable performance.

A fungal acid protease and amylase mixture disrupts microalgal cell walls to extract lipids and release monomer sugars under mild conditions.

Site-directed mutations improve enzyme solubility and shift pH optimum, reducing spontaneous inulin degradation.

Engineered bacteria with optimized enzyme sequences resolve low catalytic activity bottlenecks, significantly boosting 13-HOD production yields.

Varying molecular weight distributions in a protein mixture resolve the contradiction between production capability and mechanical strength.

Multi-stage fermentation uses sequential microbial stages to produce fermented liquid with precise acidity and particle size.

Recycling preparative liquid chromatography resolves retention time differences between 3,6-anhydro-L-galactose and other sugars to achieve high purity.

Modified enzyme binding pocket enables short alkyl tail beta-lactone synthesis, overcoming natural synthetase limitations to improve PHA crystallinity.

Incorporating biobased styrene monomers into rubber compositions to balance viscoelastic properties.

Quick drying and enzymatic treatment boost vanillin yield while maintaining complex aroma.

Tomato hybrid PX 02490003 stabilizes genetic composition by developing homozygous inbred parent lines, ensuring predictable trait expression.

Enzymatic digestion separates corn kernels into solid starch, pericarp, and liquid glucose fractions using pectinase and cellulase.

Thermostable hemicellulase variants enhance hydrolytic efficiency while lowering enzyme production costs.

Sequential Ro-GBE and Gt-GBE enzymes modify starch into short-clustered dextrin, increasing slow digestible starch content without ethanol washing.

Mutant CadA enzyme maintains catalytic activity across wider pH ranges through specific amino acid substitutions.

A donor DNA preparation using an exonuclease and single-stranded DNA binding protein enables homologous recombination cloning.

A microbiologically induced reaction forms carbonate precipitates in porous materials using oxidizable organic carbon sources.

Engineered tryptophan decarboxylases catalyze substituted tryptophan substrates to produce tryptamine analogs.

Metagenomic cloning extracts DNA from extreme environments to produce thermostable cellulases, bypassing slow culture-dependent discovery.

Etherification of poly alpha-1,3-glucan under alkaline conditions produces diverse polysaccharide derivatives with controlled substitution levels.

Hybrid corn variety CH350141 utilizes cytoplasmic male sterility for controlled pollination.

Mimivirus-derived galactosylhydroxylysyl glucosyltransferase enables bacterial production of recombinant human collagen.

slc24a5 gene mutation lightens zebrafish pigmentation to improve fluorescent protein visualization and breeding reliability.

Microbial cells synthesize medium chain length polyhydroxyalkanoate copolymers using fatty acid substrates and beta-oxidation inhibitors.

Engineered microbes convert chitin to polyactive carbohydrates, eliminating harsh chemicals and shellfish allergens.

Deleting the cryptochrome photolyase FAD-binding domain resolves cultivation complexity trade-offs while raising biomass and lipid output.

Curcumin replaces antibiotics to boost beta-carotene titer by 220% while cutting 7,8-dihydro-beta-carotene impurities by nearly 70%.

Fermented rice bran and malt juice detoxify lacquer sap at controlled temperatures.