Hydrolases TRIZ Cases

An adenosine deaminase paired with a Cpf1 nickase converts adenine to inosine, extending correction to G-to-A and C-to-T mutations.

Targeted substitutions at esterase positions 210 and 172 support more efficient PET hydrolysis while maintaining stability at elevated temperatures.

A CD71-targeting crRNA and fluorescent DNA probes simplify sensitive screening of bi-allelic edited cells.

Blocked nucleic acid molecules relay cleavage between two ribonucleoprotein complexes, amplifying signals without target amplification or primer-dimerization.

CRISPR-Cas editing of the CRN gene controls maize meristem growth, increasing kernel row number without disorganized development.

Shortening the U6 promoter creates vector space for more or larger siRNA, miRNA, and CRISPR guide sequences.

Ligand binding and protease cleavage connect extracellular sensing to RNA-guided control of endogenous and exogenous genes.

Polyadenine tracks in lysine codons tune ribosome stalling for finer control of protein expression and mRNA stability.

Mutated Cpf1 polypeptides expand PAM recognition beyond fixed sequences, enabling more versatile and targeted genome editing.

Traditional library quantification is time-consuming; adapter-targeted dCas binding normalizes samples within 15% for accurate sequencing.

CRISPR guides selectively inactivate mutant ELANE in hematopoietic stem cells, preserving the wild-type allele to improve neutrophil production.

A transient fluorescent reporter links base-editing activity to cell selection, enabling HDR-free enrichment of isogenic edited populations.

Pollen delivery bypasses tissue-culture transformation limits while producing edited haploid progeny for breeding.

Manual emasculation makes large-scale wheat hybrid seed production labor-intensive; conditional male sterility enables controlled outcrossing.

Acid phosphatase converts REE sulfates or chlorides into phosphates at room temperature, reducing heating and acid waste.

Synthetic proteotypic peptide standards enable mass-spectrometry measurement of multiple PD-L1 axis proteins beyond heterogeneous IHC scoring.

Photoperiod-sensitive Ghpsm5 sterility helps cotton breeding avoid hand emasculation while preserving female fertility for hybrid seed production.

See how plant genetic constructs use host-factor siRNAs to suppress endogenous gene silencing and sustain transgene expression.

CRISPR/Cas9 guide RNAs disrupt inhibitory receptors in engineered T cells to resist exhaustion while preserving tumor-antigen killing.

CRISPR/Cas cuts circular adenoviral DNA at ITR ends before transfection, enabling 30–50 times more efficient rescue without bacterial vector sequences.

HLA-indexed iPSC banks pair compatible donor cells with CAR-T production to address immune rejection and accelerate off-the-shelf cancer therapy.

Targeted Y256, R314, Q414, and R654 mutations reduce off-target editing while preserving robust on-target Cas9 activity.

Reducing PILS6 expression alters crown roots, lateral roots, and stalk height while improving salt tolerance in maize and other Poaceae plants.

Targeted Cas9 nicking and local helicase unwinding improve probe access for accurate detection of sequences in complex genomes.

Egg-cell-specific BBM expression induces haploid embryos without pollination, then chromosome doubling supports genetically fixed crop plants.

A locked hairpin stabilizes CRISPR gRNAs, preventing misfolding and enabling editing of targets refractory to standard Cas9 cleavage.

To address castor oil’s limited availability and unsaturation, this process uses diverse oils to produce higher-functionality biopolyols.

CRISPRa screens identify T cell regulators that increase cytokine production despite cancer-cell immune suppression.

Targeted double-strand breaks guide multiple transgenic sequences into defined plant genomic regions for linked modifications and copy-number control.

BMC01-BMC15 nucleases remove the tracrRNA requirement, simplifying genome editing while reducing human immunogenicity.

Gene-pathway engineering combines glycerol utilization and product modules in one strain, reaching productivity up to 2.1 g/L/hr.

Low-temperature enzyme mixing, polysaccharide addition, and controlled melting improve dispersion while preserving enzyme activity.

This case inhibits OsBCAT2 and soybean BCAT1/BCAT2 to accumulate branched-chain amino acids and improve drought survival and yield.

Enzyme prehydrolysis and thermal splitting improve fatty acid conversion.

Engineered Cas9 and reverse transcriptase modules improve DNA editing efficiency and help reduce indel formation in prime editing.

Lipid-formulated modified RNA enables sustained DNASE production, addressing short enzyme half-life and frequent dosing in cfDNA diseases.

A reverse transcriptase-endonuclease protein and template RNA enable targeted genome insertion without double-strand breaks.

This Cas9 case uses ligand-dependent inteins to delay nuclease activity and reduce off-target DNA cleavage and cellular toxicity.

A racemase, hydantoinase, and carbamoylase sequence converts mixed hydantoins into L-glufosinate P-esters with fewer synthetic steps.

Specific enzyme mutations improve fatty ester titers and reduce beta hydroxy esters in simplified recombinant host systems.

A fused activation peptide and protease sites support active, inhibitor-binding factor Xa while improving recombinant protein expression.

OgRRS and CgRRS guide RNA-directed excision of the INIR20 locus, reducing genetic disruption while preserving desired soybean traits.

Segmented guide RNAs use linkers and restriction cleavage to quantify spacer purity, supporting GMP production and lower off-target risk.

GeoCas9 fusion proteins combine deaminase and UGI domains to broaden PAM access, improve stability, and limit off-target editing.

A urease-activated resin composition enables pH-controlled polymerization for durable coatings without two-component mixing or VOCs.

L-amino acid isomerase and acyltransferase replace toxic racemization and costly chiral resolution in scalable D-amino acid production.

Reduced-activity RNase E limits RNA degradation, supporting toxic protein production while maintaining host cell fitness.

This case uses a null JAG1 mutation to alter leaf development, increasing seeds per kilogram while addressing seed-weight tradeoffs.

Engineered surfaces barcode DNA and RNA modifications for multiplexed profiling.

This case uses 5′-extended guide RNA with added guanines to improve base-editing range and efficiency beyond positions 13–17.