Hydrolases TRIZ Cases

An engineered PET-degrading polypeptide breaks polyester into MHET, TPA, and EG, enabling cleaner recycling without quality loss.

Engineered polypeptides break down PET into MHET, TPA, and EG, enabling closed-loop recycling without downcycling or harsh chemical impact.

An engineered polypeptide hydrolyzes PET into MHET, TPA, and EG, enabling cleaner polyester recycling under mild conditions.

Enzymatic PET breakdown yields MHET, TPA, and EG for polyester reuse, avoiding downcycling and harsh chemical recycling.

Single-bioreactor enzymatic conversion of fructose 6-phosphate cuts hexose production cost by reducing separations and avoiding ATP and NAD(P)(H).

CRISPR editing disrupts MHC-I and MHC-II while adding PD-L1 and HLA-E to reduce rejection and improve post-engraftment cell survival.

crRNA-guided MAD7 improves targeted DNA cleavage in plant cells, enabling more precise indel editing than conventional insertion methods.

Humanized MYOC loci created with CRISPR improve glaucoma phenotype replication by raising MYOC expression and intraocular pressure.

Proteases, carbohydrolases, and DNases break down anaerobic biofilms on metal surfaces to reduce MIC in oil and gas infrastructure.

Guide RNA and an RNA-dependent DNA endonuclease excise selectable markers and duplicated transgenes from INIR6 maize loci for cleaner breeding.

Short RNA substrates are ligated into non-natural oligonucleotides to improve yield, purity, and scale beyond solid-phase synthesis.

Small archaeal-derived SMART nucleases keep RuvC and HNH cleavage functions while reducing Cas effector size for more practical therapeutic delivery.

Using 16-18 nt crRNA, CasΦ cleaves duplicated genes with 1-2 mismatches, enabling multi-gene editing with fewer guides.

Enzyme treatment with protein deamidase and transglutaminase preserves plant protein solubility after drying and improves redispersion stability.

A. rhizogenes delivers Cas9 and developmental regulators to overcome poor Brassica root regeneration and raise transformation efficiency.

CRISPR guides target heterozygous ELANE SNPs to disable the mutant allele while preserving the functional copy for SCN or CyN treatment.

CRISPR-Cas editing of the MAX1 gene improves branching, seed size, and seed number while avoiding complex multi-gene breeding programs.

Novel genomic safe harbor sites enable precise CRISPR-HDR transgene insertion with stable expression and minimal disruption of adjacent genes.

A two-step HDR workflow uses temporary selection markers, then removes them to enable scarless genome edits without silent mutations.

Gene-edited hematopoietic cells with reduced HLA-A and HLA-B expression lower immune rejection and improve allogeneic engraftment.

CRISPR-edited LRR-RLK alleles expand maize meristems to raise kernel row number while preserving organized growth and ear length.

Non-viral RNP delivery with oligonucleotide tagging enables sensitive, patient-specific off-target detection in primary human T cells.

Cas12a orthologs expand PAM targeting and keep plant genome editing efficient at lower temperatures without heat-shock limits.

Cas13-crRNA detection converts SARS-CoV-2 RNA recognition into a reporter signal for rapid field screening without PCR lab workflows.

dSaCas9 binds human DNA before MDA, staying in place during Φ29 polymerase activity to enrich pathogen DNA for sequencing.

Selective serine-to-cysteine mutations create PEG attachment sites on DNase1L3, extending serum half-life while preserving nuclease activity.

Multi-site MHC editing plus PD-L1 and HLA-E expression helps donor cells evade rejection, resist NK lysis, and survive engraftment.

Modified AAV capsids improve cross-species transduction, CNS access, kidney targeting, and antibody evasion with lower dosing.

FasL expression plus FasR reduction helps allogeneic CAR-T cells kill rejecting host cells and resist AICD for longer persistence.

Heterologous regulatory sequences linked to Rps genes help soybean maintain resistance to shifting Phytophthora sojae races and protect yield.

A two-vector gene drive modifies essential genes and adds rescue transgenes to enable robust, reversible population replacement across species.

A plasmid RSS library with degenerate bases enables unbiased, high-throughput mapping of RAG sequence specificity without in vitro purification.

Cas9-guided barcode insertion in phage genomes enables high-throughput knockout screening to map conditional gene essentiality.

Using APS instead of PAPS, this case shows a closed-loop sulfonation cycle that removes toxic byproducts, simplifies synthesis, and cuts cost.

Genetically modified worms or bacteria express PFAS-degrading enzymes and self-propagate in contaminated soil, cutting remediation cost and labor.

Novel QTX125 crystalline forms and lysine adducts improve saline solubility and aqueous photostability for pharmaceutical formulation.

AAV6 donor templates paired with CRISPR/Cas9 raise HDR gene correction in primary keratinocytes and restore collagen VII expression for RDEB.

Genetic suppression of RFX, CIITA, B2M, and CD58 lowers immune recognition, reducing alloreactive T cell cytotoxicity in cell therapy.

Reversible active-site inhibitor polypeptides block premature deamidase activation, improving recombinant expression while preserving host cell viability.

GalNAc end-modified oligonucleotides improve hepatocyte targeting and stability, enabling more effective ADAR-mediated RNA editing.

ABC transporters export HMOs from engineered yeast cells to raise yield and purity during production of 2′-fucosyllactose and related oligosaccharides.

Cas9 nickase fused with nucleotide deaminase enables precise base correction without double-strand breaks, reducing deletion and rearrangement risk.

Dual PEgRNAs excise FXN GAA repeat expansions to restore frataxin expression and improve mitochondrial function in Friedreich's ataxia.

Optimized pH and EDTA conditions make RNase MC1 and Cusativin cleavage more site-specific and reproducible for higher-confidence RNA LC-MS mapping.

Engineered host cells use CDS, UGT, C11 hydroxylase, and EPH enzymes to replace extraction and complex synthesis for scalable mogroside production.

Adding phosphate near phytase before steam pelleting helps prevent heat inactivation and improves recovered enzyme activity in feed pellets.

Pre-modified Cbl-b−/− immune cells resist TGF-β suppression, sustain tumor killing, and avoid residual exogenous DNA.

A Bacillus gibsonii protease paired with a bleach catalyst lifts egg and tea stains in low-temperature, short-cycle dishwashing.

A protector sequence and secondary structure help guide RNA resist exonucleases while improving CRISPR-Cas9 targeting specificity.

Ancestral sequence reconstruction narrows lipase screening to variants that retain activity under surfactants and improve detergency in cleansing use.

Recombinant microbial hosts express targeted CYP450 and UGT enzymes to resolve unknown enzyme mechanisms while enhancing mogroside production efficiency.

Chimeric phytases maintain catalytic activity during feed pelleting, resolving the contradiction between thermal stability and enzyme function.

Engineered rat models with MFN2 mutations reproduce progressive axonal neuropathy, enabling pathogenesis research without extensive de novo development.

A biolistic method delivers proteins and genetic materials into plant cells using pressurized microparticles.

Phytase hydrolyzes phytates in plant proteins to reduce astringency and beany flavors, eliminating the need for stabilizers.

Targeting the Artemis gene creates accurate radiosensitive immunodeficiency models, resolving limitations in existing transgenic systems.

Engineering CAR Tregs with deleted CD2 prevents conversion to effector cells and resists conditioning regimens.

Site-specific nucleases integrate DNA into ideal maize genomic loci, resolving random insertion variability and improving agronomic performance.

A porcine-specific sgRNA library enables high-throughput functional gene screening using lentiviral delivery.

A dual-cassette gene vector uses a cytomegalovirus core fragment to boost transcription efficiency between units.

Lipid acyltransferase enzymes convert phospholipids into neutral fats during water degumming, reducing gum phase viscosity and increasing oil yield.

A method for preparing random sgRNA libraries uses restriction enzyme cleavage to ligate sgRNA skeletons and acquire protospacer sequences.

Reducing non-homologous end joining activity with YKU gene knockdown allows efficient homologous recombination for precise targeted integration.

Thermomyces lipase hydrolyzes hydrogenated castor oil in organic solvent at 35-60°C, resolving enzyme inactivation and high cost trade-offs.

Optimized promoter parameters resolve reliability issues when porting CRISPRa from E. coli to Pseudomonas putida, enabling precise metabolic engineering.

Disposable polyester capsules use esterase degradation to mark alpha radiation sites, eliminating complex electronic instruments and operator exposure risks.

Hydrolyzing silica monomers on enzyme surfaces creates thin porous networks that resolve mass transfer limitations while maintaining high reaction rates.

Substituting Rubisco with PEP carboxylase bypasses slow enzyme turnover to increase carbon fixation rates.

A CRISPR-Cas effector protein paired with a reverse transcriptase modifies target nucleic acids through extended guide RNA templates.

Exogenous Cas1 and Cas2 genes enable natural spacer acquisition in non-adapting CRISPR-Cas systems.

Mobile augmented sgRNAs bypass somatic cell limitations by moving into the meristem, increasing heritable gene edit frequency in plants.

Modified enzymes hydrolyze resistant stereoisomers and pyropheophytin, reducing processing costs.

Altering polynucleotide sequences encoding lipase propeptides boosts active enzyme yield to resolve low productivity constraints in detergent manufacturing.

Site-directed mutagenesis creates stable soluble PGA variants that resolve immobilization trade-offs, enabling high-yield protected insulin production.

Collagenase IV and DNase I digest frozen testicular tissue to expose seminiferous tubules, reducing cell damage caused by trypsin during SSC separation.

Urease catalyzes urea hydrolysis to raise pH and trigger rapid crosslinking, eliminating thermal feedback constraints for thin-layer curing.

Dual enzyme system reduces 3-trifluoromethyl-2-cyclohexen-1-one to high-purity (1R,3R)-isomer without large separation devices.

Magnesium carbonate and aluminum oxide precipitate host cell proteins and DNA, reducing chromatography load while preserving antibody integrity.

CRISPR/Cas9 gene editing targets a specific nucleotide sequence to generate zebrafish lines lacking intermuscular bones.

Solid support immobilization enables continuous DNA linearization without hazardous solvent purification or heat inactivation steps.

Hydroxylase enzyme catalyzes mediator oxidation to quantify 25-hydroxyvitamin D, resolving precision versus time trade-offs in clinical testing.

Targeted gene integration of CRS inhibitor genes into primary immune cells reduces cytokine release syndrome risk while enhancing therapeutic potential.

CAST workflow eliminates cell expansion and centrifugation to accelerate gene editing throughput while maintaining high viability across multiple cell lines.