Microorganisms TRIZ Cases

Fusing cell adhesion domains to laminin E8 eliminates mouse-derived Matrigel dependency, enabling safe feeder-free culture of human stem cells.

Overexpressing SLT2 in the cell wall integrity pathway increases yeast tolerance to isobutanol toxicity, improving production efficiency.

Lactobacillus helveticus KD-3 removes multiple heavy metals from goat milk powder using specific resistance genes.

SDhep I and SDhep II enzymes hydrolyze heparin substrates with high specificity.

An artificial series promoter system regulates gene expression in Bacillus subtilis through inducer-controlled repressor binding.

YE1358 and YE1615 yeast strains increase ethanol production by tolerating elevated temperatures, reducing residual glucose.

Ligand affinity chromatography stratifies heterogeneous microparticles to resolve unknown origin and enable precise diagnostic characterization.

Rhodococcus biphenylivorans nitrile hydratase hydrolyzes nitriles to amides without damaging sensitive functional groups or forming carboxylic acid by-products.

Substituting the 137th amino acid in acetohydroxy acid synthase releases feedback inhibition, increasing L-valine yield by 20.3%.

C-terminal positively charged amino acids boost IL-15 expression in E. coli twenty-fold without altering cellular activity.

Disrupting nonhomologous pathways in fungal hosts forces homologous integration, eliminating positional effects and ensuring consistent enzyme expression.

Strain J11500 combines distinct parental genotypes to accelerate yield timing and increase crop output.

A transformant integrates marker genes at chromosome ends to enable stable duplication of large Aspergillus regions.

Specific dehydratases convert 4-hydroxy-2-butanone to 3-buten-2-one, replacing harsh chemical processes with sustainable enzymatic pathways.

Biosurfactant-producing microbes emulsify adsorbed hydrocarbons in flooded soil, enabling degradation by degrading strains.

A transparent dual-layer culture substrate composed of a porous cellulose derivative membrane and polymer microfibers enables real-time optical microscopy observation.

A cell culture substrate features a surface modification layer formed by chemical vapor deposition of formyl[2.2]paracyclophane.

Amino acid substitution at position 111 creates a variant enzyme that produces O-acetyl homoserine, avoiding mixed stereoisomers from chemical synthesis.

Engineered E. coli THEE converts glucose into L-theanine via integrated metabolic pathways, achieving 75 g/L titer while reducing by-product inhibition.

Heat-inactivated Lactobacillus plantarum K37 resolves inadequate Th2 suppression by increasing IFN-gamma and decreasing IL-4 levels.

A multi-strain yeast probiotic composition combines Saccharomyces and Kluyveromyces species to enhance gut barrier function.

Bacillus amyloliquefaciens and licheniformis produce enzymes that degrade non-digestible nutrients, reducing intestinal viscosity and bacterial translocation.

Adding a protease inhibitor after collagenase treatment prevents cell damage during serum-free chondrocyte culture.

AV12 Saccharomyces cerevisiae strain produces high glycerol and aromatic compounds during fermentation.

Multi-step enzyme reaction converts unsaturated fatty acids into oxo fatty acids, resolving position-specific carbonyl group introduction challenges.

Engineered microbes replace petroleum extraction to boost methyl laurate yield while minimizing environmental damage and by-products.

Optimized fermentation and heat inactivation maximize HBsAg expression within safe limits, reversing immune tolerance.

Deleting vdh and vr genes in Actinomycetales prevents vanillin consumption, achieving 2.3-fold higher concentration.

Bacteria metabolize pesticides to detoxify environments, enhancing honeybee and silkworm resilience against chemical stressors.

A cell culture method maintains oxygen supply through controlled stirring power and dissolved oxygen concentration.

Reducing HrrSA system activity and enhancing Tat secretion genes increases heterologous protein yield while managing metabolic burden.

A fiber-optic probe system measures reflected light intensity through a transparent window to monitor biological cultures.

A homokaryotic Agaricus bisporus line enables creation of hybrid mushroom cultures with enhanced productivity and disease resistance.

A Faecalibacterium prausnitzii bacterial strain administered alongside anti-PD-1, anti-PD-L1, or anti-CTLA-4 antibodies to restore immune response.

A Citrobacter werkmanii ansB knockout mutant improves biofilm formation capacity on polypropylene surfaces.

Engineered yeast expresses UDP-glucose dehydrogenase to bypass expensive external substrate costs and boost glucuronide productivity.

Optical confinement isolates individual ribosomes from bulk mixtures to resolve synthesis kinetics and fidelity without losing single-molecule information.

An actuator substrate with transparent electrodes and an electroactive polymer layer enables precise mechanical vibration of biological cells.

Tableted cell culture media dissolve rapidly in water, eliminating hazardous dust generated by dry powder handling.

High-pressure steam controls rice viscosity to prevent agglomeration, enabling effective Koji mold penetration and improved flavor.

Segmenting the Rfp1 locus removes linkage drag that reduces yield, enabling high-performance P-CMS restoration without agronomic penalties.

Targeted residue substitution increases xylanase catalytic efficiency, reducing industrial production costs.

Segmenting metabolic functions across two non-GMO yeast strains merges biotransformation steps, eliminating intermediate isolation to boost yield.

Suppressing ku70 and ku80 genes in Aspergillus sojae and oryzae overcomes low recombination rates, enabling efficient gene disruption.

Deleting the tubulin gene in Trichoderma strains overcomes glucose inhibition to boost cellulase and xylanase yields during biomass saccharification.

A Penicillium-derived polyol oxidase catalyzes specific oxidation reactions to produce rare sugars with high yield.