Construction method of gouty arthritis animal model

Abstract

The invention discloses a construction method for a gouty arthritis animal model. The construction method comprises the following steps of (1) preparation of urate crystal suspension; (2) establishment of a hyperuricemia basic animal model; and (3) establishment of the gouty arthritis animal model. In the construction method, by selecting an SPF chick as an experimental animal, a hyperuricemia model is established by feeding the SPF chick with high-protein and high-calcium feed, the urate crystal suspension is injected into a knuckle articular cavity of a hyperuricemia model chick, a microenvironment in vivo of a patient with gouty arthritis is simultated to the maximum, and the gouty arthritis animal model which more accords with a human microenvironment in vivo is established.

Description

technical field

[0001] The invention relates to the field of medical animal model research, in particular to a method for constructing a gouty arthritis animal model. Background technique

[0002] Gouty arthritis is an inflammatory response disease caused by the deposition of monosodium urate crystals (MUC) in the internal and external tissues of the joints due to the disorder of purine metabolism. The main clinical manifestations are recurrent joint redness, swelling, heat, pain and dysfunction, which may cause joint deformity in severe cases. The pathogenesis is that the uric acid concentration in the joint is supersaturated to form crystals, and the shed crystals trigger the body's innate immune response, causing the activation of mast cells and monocyte-macrophage systems, and secrete interleukins through a series of complex intracellular cascade reactions. necrotic factor, triggering acute inflammatory response. Acute attacks are self-limiting, but they are prone to r...

Examples

Embodiment 1

[0018] Embodiment 1 constructs gouty arthritis animal model according to the following method

[0019] 1. Preparation of urate crystal suspension:

[0020] Step 1: Put the gout stones obtained from the hands and feet of gout patients during the operation to dry in an incubator, then put the white crystals into a clean beaker, put in an appropriate amount of distilled water, and stir for a few minutes with a glass rod until it is basically completely Dissolve, filter out the sediment and impurities with a filter paper on the funnel pad, and then put the solution in an incubator to dry to obtain urate crystals. After the urate crystals are ground and pulverized, they are filtered through a metal mesh sieve with a pore size of 250um to prepare purified urate crystals for future use.

[0021] Step 2: Put the above-mentioned purified urate crystals in a high-temperature sterilization box at 180°C for 3 hours, then wash them with ethanol with a volume fraction of 95%, centrifuge th...

Embodiment 2

[0026] Embodiment 2 Gouty arthritis animal model evaluation

[0027] 1. Experimental grouping

[0028] 120 healthy 30-day-old male SPF chickens, weighing (500±50) g, were randomly divided into 3 groups, blank control group (group A), model group 1 (group B), model group 2 (group C), each group 40 only.

[0029] 2. Modeling method

[0030] Group A: fed with common feed, and injected 0.5ml of distilled water into the left knee joint cavity after 2 weeks.

[0031] Group B: fed with special feed, and injected 0.5ml of distilled water into the left knee joint cavity after 2 weeks.

[0032] Group C: fed with special feed, 0.5ml of urate crystal suspension with a mass concentration of 100mg / ml was injected into the left knee joint cavity after 2 weeks.

[0033] After all the injections were completed, the knee joints were flexed, extended, and rotated for 10 minutes. Continue to feed each group for 1 week.

[0034] Special feed refers to high-protein and high-calcium feed, and ...

technical field

[0001] The invention relates to the field of medical animal model research, in particular to a method for constructing a gouty arthritis animal model. Background technique

[0002] Gouty arthritis is an inflammatory response disease caused by the deposition of monosodium urate crystals (MUC) in the internal and external tissues of the joints due to the disorder of purine metabolism. The main clinical manifestations are recurrent joint redness, swelling, heat, pain and dysfunction, which may cause joint deformity in severe cases. The pathogenesis is that the uric acid concentration in the joint is supersaturated to form crystals, and the shed crystals trigger the body's innate immune response, causing the activation of mast cells and monocyte-macrophage systems, and secrete interleukins through a series of complex intracellular cascade reactions. necrotic factor, triggering acute inflammatory response. Acute attacks are self-limiting, but they are prone to r...

Examples

Embodiment 1

[0018] Embodiment 1 constructs gouty arthritis animal model according to the following method

[0019] 1. Preparation of urate crystal suspension:

[0020] Step 1: Put the gout stones obtained from the hands and feet of gout patients during the operation to dry in an incubator, then put the white crystals into a clean beaker, put in an appropriate amount of distilled water, and stir for a few minutes with a glass rod until it is basically completely Dissolve, filter out the sediment and impurities with a filter paper on the funnel pad, and then put the solution in an incubator to dry to obtain urate crystals. After the urate crystals are ground and pulverized, they are filtered through a metal mesh sieve with a pore size of 250um to prepare purified urate crystals for future use.

[0021] Step 2: Put the above-mentioned purified urate crystals in a high-temperature sterilization box at 180°C for 3 hours, then wash them with ethanol with a volume fraction of 95%, centrifuge th...

Embodiment 2

[0026] Embodiment 2 Gouty arthritis animal model evaluation

[0027] 1. Experimental grouping

[0028] 120 healthy 30-day-old male SPF chickens, weighing (500±50) g, were randomly divided into 3 groups, blank control group (group A), model group 1 (group B), model group 2 (group C), each group 40 only.

[0029] 2. Modeling method

[0030] Group A: fed with common feed, and injected 0.5ml of distilled water into the left knee joint cavity after 2 weeks.

[0031] Group B: fed with special feed, and injected 0.5ml of distilled water into the left knee joint cavity after 2 weeks.

[0032] Group C: fed with special feed, 0.5ml of urate crystal suspension with a mass concentration of 100mg / ml was injected into the left knee joint cavity after 2 weeks.

[0033] After all the injections were completed, the knee joints were flexed, extended, and rotated for 10 minutes. Continue to feed each group for 1 week.

[0034] Special feed refers to high-protein and high-calcium feed, and ...

Images