Application of Lactobacillus plantarum LPJZ-658 in improving growth performance, meat quality and disease resistance of broiler chickens

Abstract

The application of Lactobacillus plantarum LPJZ‑658 in improving the growth performance, meat quality and disease resistance of broilers relates to the field of microbial agents. The Lactobacillus plantarum LPJZ‑658 was deposited in the General Microbiology Center of the China Microbiological Culture Collection Administration on July 16, 2021, with a deposit number of CGMCC No. 22908. The present invention found that Lactobacillus plantarum LPJZ‑658 can significantly increase the average daily feed intake and average daily weight gain of broilers, significantly reduce the feed-to-weight ratio, significantly reduce the liver / body weight ratio and intermuscular fat width, significantly increase the pH value and meat color of the breast muscle, significantly reduce the cooking loss of the breast muscle, significantly improve the color of the leg muscle, significantly reduce the malondialdehyde content in the immune hind leg muscle, significantly increase the level of superoxide dismutase in the immune hind leg muscle, significantly increase the height of the duodenal and ileal villi and the ratio of the villus crypts, and significantly increase the level of IgG antibodies in the serum.

Description

Technical Field

[0001] The invention relates to the technical field of microbial agents, and in particular to application of Lactobacillus plantarum LPJZ-658 in improving the growth performance, meat quality and disease resistance of broiler chickens. Background Art

[0002] Broilers are the largest source of chicken food for people's daily needs. Chicken can provide various essential amino acids, fatty acids, vitamins and other nutrients for human growth and development, and is one of the important sources of protein and phospholipids in the Chinese diet. In my country, chicken is the second most consumed meat after pork, and the white-feathered broiler industry is the most scaled-up industry in my country's livestock and poultry breeding industry. However, large-scale breeding shortens the feeding cycle of broilers, and the quality of chicken will also be affected to a certain extent.

[0003] In order to ensure the normal growth of broilers and reduce the occurrence of diseases, antibiotic additives have been added during the feeding process to promote growth, improve feed conversion rate and improve growth performance. However, excessive use of antibiotics may lead to bacterial resistance to antibiotics and antibiotic residues in animal foods. In recent years, the problem of antibiotic residues in livestock and poultry products has also attracted widespread attention. The nutritional quality of chicken can be improved by controlling the feeding cycle, but large-scale farming and market demand have shortened the time for broilers to be marketed. At the same time, intensive farming and "antibiotic ban" and "antibiotic restriction" have affected the disease resistance and growth performance of poultry. The quality of broilers can be improved through variety selection and environmental control, but it is often difficult to popularize due to breeding costs and conditions. In response to the above series of problems, in the context of antibiotic restriction and prohibition, exploring an economical and efficient ecological breeding method for broilers is the primary task currently faced.

[0004] In recent years, countries around the world have realized the harm of antibiotic abuse, and antibiotic-free breeding has become the development direction of green and healthy breeding in the broiler industry. How to improve the growth performance, meat quality and disease resistance of broilers through green, healthy and pollution-free antibiotic substitutes, thereby reducing the losses caused by "antibiotic ban", is the focus of my country's broiler industry and the industry. Summary of the invention

[0005] In order to improve the growth performance, meat quality and disease resistance of broilers, the present invention provides an application of Lactobacillus plantarum LPJZ-658 in improving the growth performance, meat quality and disease resistance of broilers.

[0006] The technical solution adopted by the present invention to solve the technical problem is as follows:

[0007] The invention discloses an application of Lactobacillus plantarum LPJZ-658 in improving the growth performance, meat quality and disease resistance of broiler chickens.

[0008] As a preferred embodiment, the Lactobacillus plantarum LPJZ-658 was deposited in the General Microbiology Center of China National Microbiological Culture Collection Administration on July 16, 2021, with the deposit number CGMCC No. 22908.

[0009] As a preferred embodiment, the Lactobacillus plantarum LPJZ-658 is a freeze-dried powder.

[0010] As a preferred embodiment, the addition amount of the Lactobacillus plantarum LPJZ-658 freeze-dried powder is 2.6×10 9 cfu / g.

[0011] As a preferred embodiment, the preparation method of the Lactobacillus plantarum LPJZ-658 freeze-dried powder is:

[0012] Lactobacillus plantarum LPJZ-658 was inoculated into MRS liquid medium at a 1% inoculation rate, cultured at 37°C for 16h-24h, activated and cultured for three generations in the same manner, centrifuged, and the bacterial sludge was collected; a freeze-drying protective agent was added at a weight ratio of 1:6, mixed, and placed in a refrigerator for precooling, and then placed in a vacuum freeze dryer for freeze drying to obtain a viable bacterial count of not less than 10 11 cfu / g of Lactobacillus plantarum LPJZ-658 freeze-dried powder.

[0013] As a preferred embodiment, the lyoprotectant comprises: 25% wt skim milk, 8% wt lactose and 1% wt glycerol.

[0014] As a preferred embodiment, the functions of Lactobacillus plantarum LPJZ-658 include:

[0015] (1) Increase the average daily feed intake and average daily weight gain of broiler chickens;

[0016] (2) Reduce the feed-to-weight ratio of broilers;

[0017] (3) Reduce the liver / body weight ratio and intermuscular fat width of broiler chickens;

[0018] (4) Improve the pH value and meat color of broiler breast muscle after slaughter, reduce the cooking loss of broiler breast muscle after slaughter, and improve the muscle color of broiler leg after slaughter;

[0019] (5) reduce the malondialdehyde content in the leg muscles of broiler chickens after immunization and increase the level of superoxide dismutase in the leg muscles of broiler chickens after immunization;

[0020] (6) Improve the height of duodenum villus, ileum villus, and villus height / crypt depth ratio of broiler chickens;

[0021] (7) Increase the level of IgG antibodies in broiler serum.

[0022] The beneficial effects of the present invention are:

[0023] The present invention verifies through experiments that Lactobacillus plantarum LPJZ-658 can significantly increase the average daily feed intake and average daily weight gain of broilers, significantly reduce the feed-to-weight ratio of broilers, significantly reduce the liver / body weight ratio and intermuscular fat width of broilers, significantly increase the pH value and meat color of breast muscle 24 hours after slaughter, significantly reduce the cooking loss of breast muscle, significantly improve the color of leg muscle, significantly reduce the malondialdehyde content in the leg muscle of broilers after immunization, significantly increase the level of superoxide dismutase in the leg muscle of broilers after immunization, significantly increase the height of the villi of the duodenum and ileum of broilers and the ratio of the villi height to the depth of the crypt, and significantly increase the level of IgG antibodies in the serum of broilers. Therefore, Lactobacillus plantarum LPJZ-658 improves the growth performance, meat quality and disease resistance of broilers by regulating the average daily feed intake, average daily weight gain, feed-to-weight ratio, broiler liver / body weight ratio, intermuscular fat width, breast muscle pH and meat color 24 hours after slaughter, breast muscle cooking loss, leg muscle color, malondialdehyde content in immune hind leg muscle, superoxide dismutase level in immune hind leg muscle, broiler duodenum and ileum villus height and villus height / crypt depth ratio, and serum antibody level, providing new ideas for the healthy broiler breeding industry and having broad application prospects and promotion value. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 To investigate the effect of Lactobacillus plantarum LPJZ-658 (lyophilized powder) treatment on the small intestine morphology of broiler chickens. Figure 1 In the figure, A shows the microvilli morphology of duodenum of broiler chickens in different treatment groups; B shows the microvilli morphology of jejunum of broiler chickens in different treatment groups; C shows the microvilli morphology of ileum of broiler chickens in different treatment groups. DETAILED DESCRIPTION

[0025] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0026] 1 Materials

[0027] 1.1 The preparation method of Lactobacillus plantarum LPJZ-658 freeze-dried powder is as follows:

[0028] Lactobacillus plantarum LPJZ-658 was inoculated into MRS liquid medium at a 1% inoculation rate, cultured at 37°C for 16h-24h, activated and cultured for three generations in the same manner, centrifuged, and the bacterial sludge was collected; a freeze-drying protective agent (25%wt skim milk, 8%wt lactose and 1%wt glycerol, the remainder being water) was added according to a 1:6 (weight ratio) and mixed, and then placed in a refrigerator for precooling, and then placed in a vacuum freeze dryer for freeze drying to obtain a viable cell count of not less than 10 11 cfu / g of Lactobacillus plantarum LPJZ-658 freeze-dried powder, and accurately counted using MRS agar medium.

[0029] The Lactobacillus plantarum LPJZ-658 used in the present invention was deposited in the General Microbiology Center of the China Culture Collection Administration on July 16, 2021, referred to as CGMCC, with the address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing (Institute of Microbiology, Chinese Academy of Sciences), and the deposit number is: CGMCC No. 22908.

[0030] 1.2 Experimental animals

[0031] A total of 1200 healthy mixed-sex white-feathered broiler chickens of 1 day old and similar initial body weight were included.

[0032] 1.3 Commonly used instruments, reagents, and test kits

[0033] Vernier caliper, water bath, pH meter (pH-star, Matthaus, Germany), colorimeter CR-410 (photo-star, Matthaus, Germany), electronic balance, digital meat tenderizer (C-LM3B, TENOVO, Shandong, China), Scientz-48l homogenizer (Ningbo Scientz Biotechnology, Zhejiang, China), low-temperature refrigerated centrifuge, MDA and SOD detection kits (Nanjing Jiancheng Bioengineering Institute, Jiangsu, China), phosphate buffered saline (PBS), 4% paraformaldehyde fixative, HE staining reagent, chicken serum antibody ELISA detection kit (Nanjing Jiancheng Bioengineering Institute, Jiangsu, China).

[0034] 2 Methods and Results

[0035] One-day-old healthy mixed-sex broiler chickens (white-feathered broiler chickens, n = 1200) with similar initial body weight were randomly divided into 4 groups: basic diet group (CON group), basic diet + vaccine injection group (VA group), basic diet + 2.6×10 9 cfu / g Lactobacillus plantarum LPJZ-658 (lyophilized powder) group (LP group), basic diet + 2.6×10 9 cfu / g Lactobacillus plantarum LPJZ-658 (lyophilized powder) + vaccine injection group (VALP group). Each group has 30 replicates, and each replicate has 10 chickens.

[0036] On the 7th day of the experiment, the broilers in the VA and VALP groups were inoculated with a combined inactivated vaccine of Newcastle disease and avian influenza (H9 subtype) (Newcastle virus strain LaSota and avian influenza virus strain HN106) produced by China Huapai Biotechnology Group Co., Ltd. As a control, the broilers in the CON and LP groups were inoculated with an equal amount of normal saline.

[0037] Feeding and management: The feeding environment should be properly ventilated and routinely disinfected, and free access to water and food should be provided during feeding. The basic feed is formulated in accordance with the nutritional requirements of the "Nutritional Requirements of Chickens" recommended by NRC (1994), the "Chicken Feeding Standards" (NY / T33-2004) and the "China Feed Composition and Nutritional Value Table (2005)".

[0038] 2.1 Growth performance

[0039] Average daily feed intake and average daily weight gain were recorded. One broiler from each replicate was weighed on day 1 and day 42 of the experiment. Feed consumption in each replicate was monitored weekly. The experiment lasted for 42 days, and average daily feed intake, average daily weight gain, and feed-to-weight ratio (feed consumption / weight gain) were determined.

[0040] The growth performance data of 42-day-old broilers are shown in Table 1. The growth performance test and results show that adding 2.6×10 9 cfu / g Lactobacillus plantarum LPJZ-658 (freeze-dried powder) can significantly increase the average daily feed intake and average daily weight gain of broilers, and can significantly reduce the feed-to-weight ratio. This proves that Lactobacillus plantarum LPJZ-658 can significantly improve the growth performance of broilers.

[0041] Table 1

[0042] project CON Group VA group LP Group VALP group SEM P-value ADG(g) <![CDATA[56.81 b ]]> <![CDATA[59.91 ab ]]> <![CDATA[65.02 a ]]> <![CDATA[64.67 a ]]> 1.583 0.002 ADFI(g) <![CDATA[99.98 b ]]> <![CDATA[104.24 ab ]]> <![CDATA[109.23 a ]]> <![CDATA[106.71 a ]]> 2.832 0.017 FCR <![CDATA[1.76 a ]]> <![CDATA[1.74 a ]]> <![CDATA[1.68 b ]]> <![CDATA[1.65 b ]]> 0.037 0.005

[0043] In the table, ADG: average daily gain; ADFI: average daily feed intake; FCR: feed-to-weight ratio; SEM: standard error of the sample mean; shoulder marks a, b in the same column indicate significant differences (P<0.05); P-value: significant value.

[0044] 2.2 Sample collection and carcass quality testing

[0045] Fast for 12 hours before slaughter, and select one chicken (42 days old) from each replicate for weighing. After blood sampling from the heart, the broilers were euthanized, and different parts of the carcass were separated using a scalpel as samples for inspection. The width of intermuscular fat and the thickness of subcutaneous fat were measured with a vernier caliper. After removing feathers and blood, the carcass weight was measured. All organs except the heart, liver, stomach, kidney, and lung were removed, and the liver weight and half-eviscerated weight were measured. Then, all the internal organs were removed and weighed to measure the full-eviscerated weight. The slaughter rate [(carcass weight / pre-slaughter weight) × 100%], half-eviscerated rate [(half-eviscerated weight / pre-slaughter weight) × 100%], full-eviscerated rate [(full-eviscerated weight / pre-slaughter weight) × 100%] and liver-body ratio [(liver weight / pre-slaughter weight) × 100%] were calculated. The abdominal fat, left breast muscle and thigh muscle of the broiler were separated and weighed. The left breast and leg muscles were taken and stored at 4°C. The pH value, drip loss rate, meat color, cooking loss and shear force were measured. Some of the right breast and leg muscles were immediately frozen and stored at -20°C for subsequent analysis. The abdominal fat rate [(abdominal fat weight / total eviscerated weight) × 100%], breast muscle rate [(left breast muscle weight × 2 / total eviscerated weight) × 100%] and leg muscle rate [(left leg muscle weight × 2 / total eviscerated weight) × 100%] were calculated.

[0046] The carcass quality data of 42-day-old broilers are shown in Table 2. The sample collection and carcass quality test results show that adding 2.6×10 9cfu / g Lactobacillus plantarum LPJZ-658 (freeze-dried powder) can significantly reduce the liver-to-body ratio and intermuscular fat width of broiler chickens, and can significantly increase the leg muscle rate. The results showed that Lactobacillus plantarum LPJZ-658 can significantly improve carcass quality, significantly increase leg muscle rate and reduce intermuscular fat width.

[0047] Table 2

[0048] project CON Group VA group LP Group VALP group SEM P-value Carcass rate (%) 94.18 93.3 93.28 93.25 0.355 0.233 Half-eviscerated rate (%) 84.01 83.36 83.02 83.48 0.374 0.363 Eviscerated rate (%) 76.14 75.88 75.39 76.1 0.424 0.614 Abdominal fat percentage (%) 1.128 1.18 1.18 1.13 0.092 0.957 Liver / body weight ratio (%) 2.11a 1.99ab 1.813b 1.93ab 0.055 0.006 Chest muscle rate (%) 28.5 28.99 28.89 27.95 0.791 0.794 Leg muscle rate (%) 19.15b 20.39ab 20.88a 21.42a 0.41 0.003 Subcutaneous fat thickness (cm) 1.99 1.5 1.67 1.52 0.153 0.125 Intermuscular fat width (mm) 4.03a 3.41ab 3.59ab 3.17b 0.208 0.041

[0049] In the table, SEM: standard error of the sample mean; shoulders a, b in the same column indicate significant differences (P<0.05); P-value: significant value.

[0050] 2.3 Meat quality evaluation

[0051] The left breast and thigh muscles were analyzed for meat quality (e.g. pH, meat color, drip loss, cooking loss and shear force).

[0052] pH value: The pH value of muscle samples was measured using a pH meter (pH-star, Matthaus, Germany) 45 minutes and 24 hours after broiler slaughter. The calibrated probe was inserted into the meat sample at a depth of approximately 1 cm. The muscle samples were measured three times and the average value was taken as the pH value.

[0053] Meat color: Meat color was measured three times at three different locations of the muscle samples using a calibrated colorimeter (photo-star, Matthaus, Germany) at 45 minutes and 24 hours after slaughter, and the average value was recorded.

[0054] Drip loss rate: The drip loss rate of the muscle samples 24 hours and 48 hours after slaughter was measured by the hanging method. First, the moisture on the surface of the muscle samples was absorbed, and then the muscle samples were weighed. The muscle samples were placed in a sealed bag and hung with a hook and a line. The samples were then placed in a 4°C refrigerator for 24 hours and 48 hours. After being taken out, the moisture on the surface of the meat samples was absorbed with filter paper and then weighed. The drip loss rate was calculated using the following formula: drip loss rate (%) = [(initial weight - final weight) / initial weight] × 100%.

[0055] Cooking loss rate: Calculate the muscle cooking loss rate 24 hours after slaughter. Specifically, take about 100g of muscle sample, dry the surface moisture and weigh it, put the muscle sample into a glass beaker, heat it in a water bath until the core temperature of the muscle reaches 75°C and keep it for 20 minutes; after cooling to room temperature, dry the surface moisture of the muscle sample and weigh it, and calculate the cooking loss rate. The calculation formula is: Cooking loss rate = [(initial weight - final weight) / initial weight] × 100%.

[0056] Shear force: After measuring the cooking loss rate, three muscle samples of 0.25 cm (thickness) × 1 cm (width) × 2.5 cm (length) were taken and sheared perpendicular to the fiber using a digital meat tenderizer (C-LM3B, TENOVO, Shandong, China) with a crosshead speed of 5 mm / s. Each muscle sample was measured five times, and the average value was taken as the shear force.

[0057] Table 3

[0058]

[0059]

[0060] In the table, SEM: standard error of the sample mean; shoulders a, b in the same column indicate significant differences (P<0.05); P-value: significant value.

[0061] 2.4 Evaluation of antioxidant capacity in muscle

[0062] Using a Scientz-48l homogenizer (Ningbo Scientz Biotechnology, Zhejiang, China), 0.1 g of muscle tissue was used to prepare tissue homogenate (1:9, w / v) with 0.9% sodium chloride buffer and centrifuged at 4000 g for 15 min at 4°C. The malondialdehyde (MDA) content and superoxide dismutase (SOD) activity in the supernatant were determined, respectively.

[0063] The antioxidant capacity data of 42-day-old broiler muscle are shown in Table 4. 9 cfu / g Lactobacillus plantarum LPJZ-658 (lyophilized powder) can significantly reduce the content of malondialdehyde in the leg muscle of broiler chickens after immunization, and significantly increase the activity of superoxide dismutase.

[0064] Table 4

[0065]

[0066] In the table, MDA: malondialdehyde; SOD: superoxide dismutase; SEM: standard error of the mean; shoulders a, b in the same column indicate significant differences (P<0.05); P-value: significant value.

[0067] 2.5 Intestinal morphological analysis

[0068] Intestinal segments of ileum, jejunum, and duodenum were rinsed with cold phosphate-buffered saline (PBS) and fixed with paraformaldehyde (4%) for paraffin embedding. Next, the sections were stained with H&E. Five crypts and microvilli were randomly selected from each segment for the determination of villus height and crypt depth.

[0069] The intestinal morphology data of 42-day-old broiler chickens are shown in Table 5 and Figure 1 As shown in the intestinal morphology analysis test and results, it can be seen that adding 2.6×10 9 cfu / g Lactobacillus plantarum LPJZ-658 (lyophilized powder) can significantly increase the villus height of the duodenum and ileum of broiler chickens and the ratio of villus height to crypt depth.

[0070] Table 5

[0071]

[0072] In the table, VH: villus height; CD: crypt depth; SEM: standard error of the sample mean; shoulders a, b in the same column indicate significant differences (P<0.05); P-value: significant value.

[0073] 2.6 Serum antibody index

[0074] Blood was collected from the heart of broiler chickens, and serum samples were centrifuged and stored at -70°C. Chicken ELISA kits (Nanjing Jiancheng Bioengineering Institute) were used to detect serum IgM, IgG, and IgA antibody levels.

[0075] The data of serum antibody levels of 42-day-old broilers are shown in Table 5. Through the serum antibody index measurement test and results, it can be seen that adding Lactobacillus plantarum LPJZ-658 (lyophilized powder) to the basic feed can significantly increase the IgG antibody level in broiler serum.

[0076] Table 6

[0077] project CON Group VA group LP Group VALP group SEM P-value IgM (mg / ml) 31.02 39.46 32.54 38 3.216 0.229 IgG (mg / ml) <![CDATA[18.63 b ]]> <![CDATA[26.05 ab ]]> <![CDATA[27.21 a ]]> <![CDATA[29.45 a ]]> 1.832 0.0048 IgA (mg / ml) 1.28 1.3 1.3 1.53 0.098 0.262

[0078] In the table, VH: villus height; CD: crypt depth; SEM: standard error of the sample mean; shoulders a, b in the same column indicate significant differences (P<0.05); P-value: significant value.

[0079] 3 Conclusion

[0080] The average daily feed intake and average daily weight gain were recorded during the experimental period. On the first and 42nd days of the experiment, one broiler was selected from each replicate and weighed separately. The feed consumed by each pen was monitored weekly. The experiment lasted for 42 days, and the average daily feed intake, average daily weight gain and feed-to-weight ratio (feed consumption / weight gain) were measured. At the end of the experiment, ① the carcass rate, half-eviscerated rate, full-eviscerated rate, abdominal fat rate, liver / body weight ratio, breast muscle rate, leg muscle rate, subcutaneous fat thickness and intermuscular fat width were measured to evaluate the carcass quality; ② the muscle pH, meat color, drip loss, cooking loss and muscle shear force were measured to evaluate the meat quality; ③ the muscle malondialdehyde content and superoxide dismutase activity were measured to evaluate the antioxidant status of the muscle; ④ the intestinal villus height, crypt depth and villus height / crypt depth ratio were measured to evaluate the intestinal morphology; ⑤ the antibody (IgG, IgM, IgA) content in the serum was measured to evaluate the immune level.

[0081] The experimental results show that Lactobacillus plantarum LPJZ-658 significantly increased the average daily feed intake and average daily weight gain of broilers, significantly reduced the feed-to-weight ratio of broilers, significantly reduced the liver / body weight ratio and intermuscular fat width of broilers, significantly increased the pH value and meat color of breast muscle 24 hours after slaughter, significantly reduced the cooking loss of breast muscle, significantly improved the color of leg muscle, significantly reduced the malondialdehyde content in the leg muscle of broilers after immunization, significantly increased the level of superoxide dismutase in the leg muscle of broilers after immunization, significantly increased the villus height of duodenum and ileum of broilers and the ratio of villus height / crypt depth, and significantly increased the IgG antibody level in broiler serum.

[0082] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. Application of Lactobacillus plantarum LPJZ-658 in preparing a feed for improving the growth performance, meat quality and disease resistance of broilers, characterized in that: The Lactobacillus plantarum LPJZ-658 was deposited in the General Microbiology Center of the China Microorganism Culture Collection Administration on July 16, 2021, with the deposit number CGMCC No.22908.

2. The use according to claim 1, characterized in that: The Lactobacillus plantarum LPJZ-658 is freeze-dried powder.

3. The use according to claim 2, characterized in that: The amount of the Lactobacillus plantarum LPJZ-658 freeze-dried powder added is 2.6×10 9 cfu / g.

4. The use according to claim 2, characterized in that: The preparation method of the Lactobacillus plantarum LPJZ-658 freeze-dried powder is: Lactobacillus plantarum LPJZ-658 was inoculated into MRS liquid medium at a 1% inoculum amount, cultured at 37°C for 16h-24h, activated and cultured for three generations in the same manner, centrifuged, and the bacterial sludge was collected; a freeze-drying protective agent was added at a weight ratio of 1:6, mixed, and placed in a refrigerator for precooling, and then placed in a vacuum freeze dryer for freeze drying to obtain a viable bacterial count of not less than 10 11 cfu / g of Lactobacillus plantarum LPJZ-658 freeze-dried powder.

5. The use according to claim 4, characterized in that: The freeze-drying protective agent comprises: 25%wt skim milk, 8%wt lactose and 1%wt glycerol.

6. The use according to claim 1, characterized in that: The functions of Lactobacillus plantarum LPJZ-658 include: (1) Increase the average daily feed intake and average daily weight gain of broiler chickens; (2) Reduce the feed-to-weight ratio of broilers; (3) Reduce the liver / body weight ratio and intermuscular fat width of broiler chickens; (4) Improve the pH value and meat color of broiler breast muscle after slaughter, reduce the cooking loss of broiler breast muscle after slaughter, and improve the muscle color of broiler leg after slaughter; (5) reduce the malondialdehyde content in the leg muscles of broiler chickens after immunization and increase the level of superoxide dismutase in the leg muscles of broiler chickens after immunization; (6) Improve the height of duodenum villus, ileum villus, and villus height / crypt depth ratio of broiler chickens; (7) Increase the level of IgG antibodies in broiler serum.

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