A method for constructing oligosporic arthrospores that spontaneously produce predatory organs

By constructing a spontaneously producing predatory organs, the problem of low response efficiency in the prior art is solved, and the spontaneously producing predatory organs is achieved, which improves the efficiency and sensitivity of nematode biological control.

CN116179374BActive Publication Date: 2025-09-02YUNNAN UNIV
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Patent Information

Application Number
CN202211282932.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-10-19
Publication Date
2025-09-02
Estimated Expiration
2042-10-19

AI Technical Summary

Technical Problem

The prior art is difficult to construct supraspores that spontaneously produce predatory organs, resulting in low response efficiency for nematode biological control and inability to prevent nematode infection in advance.

Method used

By integrating promoter, gene of interest, GFP sequence, hygromycin screening marker and terminator into pCE-Zero vector, the fluorescent transformation fragment was transformed into at least the protoplasts of the pyramidal protoplasts by using a PEG/CaCl2-mediated method, positive transformants were screened out to obtain pyramidal sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic sporadic spor

Benefits of technology

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Abstract

The present invention provides a method for constructing a oligospora spore that spontaneously produces a predatory organ, belonging to the field of molecular biology technology. The method comprises: sequentially connecting a promoter, a target gene, a GFP sequence, a hygromycin selection marker, and a terminator, and constructing the entire construct in a pCE-Zero vector; then using primer 1 for PCR amplification to obtain a fluorescent conversion fragment; preparing oligospora spore protoplasts, and transforming the fluorescent conversion fragment into the oligospora spore protoplasts; and screening for positive transformants. The oligospora spores obtained by this method can spontaneously produce a predatory organ, greatly advance the response to nematodes, and have a better control effect on nematodes.
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