A method for preventing petal reversion in a double tulip

Abstract

The present application relates to the field of agricultural cultivation technology, and particularly relates to a method for preventing the petal reversion of double peony, comprising the following steps: soil disinfection, low-temperature germination, bulb planting, temperature regulation and light management after planting, and water and fertilizer management for regulating the growth conditions of double peony petals. The present application significantly reduces the occurrence of petal reversion of double peony by low-temperature germination, bulb planting, temperature regulation and light management after planting, guarantees the high double peony characteristics of double peony, improves the commodity traits and ornamental quality of double peony, avoids the generation of defective flowers, and improves the ornamental value and commercial value.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of agricultural cultivation technology, in particular to a method for preventing petal reversion of double peony lily. BACKGROUND

[0002] Double peony lily is a kind of lily formed by high petalization of stamens and pistils, which is similar to lotus and has peculiar flower type, no pollen contamination, and is a new favorite of lily varieties, which is loved by many consumers and has high commercial value. However, there are differences between double peony lily and conventional single peony lily in cultivation and management technology. In production, due to improper environmental control, petal reversion often occurs, that is, incomplete petalization of stamens or pistils, appearance of pistil reoccurrence and stigma deformity, or appearance of stamen reoccurrence and incomplete development, and attachment of abnormal anthers on petal edges, which seriously affects the commodity characteristics and ornamental quality of double peony lily and significantly reduces its economic value, becoming a defective flower.

[0003] Therefore, how to prevent petal reversion of double peony lily is a problem to be solved in the cultivation and management of double peony lily. SUMMARY

[0004] In order to reduce the occurrence of petal reversion of double peony lily, the present application provides a method for preventing petal reversion of double peony lily. By controlling the temperature and light conditions of the whole growth and development period of double peony lily cultivation, and assisting with corresponding water and fertilizer, soil disinfection and comprehensive cultivation management method, the occurrence of petal reversion of double peony lily is significantly reduced, and the characteristics of high petalization of double peony lily are ensured.

[0005] To achieve the above purpose, the technical scheme adopted by the present application is as follows:

[0006] The present application provides a method for preventing petal reversion of double peony lily, comprising the following steps:

[0007] Soil disinfection: the fermented cow and sheep manure is applied at 500kg / hm 2 -700kg / hm 2 Fertilization, as base fertilizer, is fully mixed with soil, and high-temperature smothering method is used for soil disinfection.

[0008] Low-temperature germination: the seed ball is placed in the substrate and low-temperature germination is carried out at 8℃-10℃, and when the bud length is 5cm-6cm, the seed ball is planted in the disinfected soil.

[0009] The substrate is a mixture of grass charcoal and perlite in a volume ratio of 1-3:1.

[0010] Environmental temperature regulation: the environmental temperature is set to 12℃-16℃ during seed ball planting to seedling emergence, the environmental temperature is set to 18℃-22℃ during seedling emergence to bud appearance, and the environmental temperature is set to 23℃-28℃ during color penetration period.

[0011] Light management: the light intensity is set to 0Lx-37000Lx in the early stage of planting; the light intensity is set to 33000Lx-42000Lx in the large seedling stage; the light intensity is set to 48000Lx-57000Lx in the stage from budding to bud coloration.

[0012] Water and fertilizer management: water is poured thoroughly when planting; compound nitrogen, phosphorus and potassium fertilizer is applied during the growth period; humic acid fertilizer and seaweed fertilizer are applied after one month of planting; chelated iron fertilizer is applied one and a half months after germination; dihydrogen potassium phosphate aqueous solution is applied when budding; chelated calcium fertilizer is sprayed on leaves when the winter temperature is low.

[0013] Netting: the net is worn on the coloration bud.

[0014] The present application clearly defines the whole growth and development period of double-flowered lily cultivation, including the precise temperature and light conditions in different stages of seed ball germination, early stage of planting, budding stage and coloration stage, and the corresponding water and fertilizer, soil disinfection comprehensive cultivation management method, which significantly reduces the occurrence of double-flowered lily petal reversion phenomenon and guarantees the high double-flowered characteristics of double-flowered lily.

[0015] Further, the soil disinfection of the shed is covered with plastic film, and after high-temperature disinfection for 7-10 days on sunny days, the plastic film is opened for ventilation for 4-6 days before use.

[0016] Further, the seed ball circumference is 14-16 cm.

[0017] Further, the substrate for low-temperature germination is a mixture of grass charcoal and perlite in a volume ratio of 2-3:1.

[0018] Further, the light management time is 8-12 hours.

[0019] Further, the soil temperature is guaranteed to be 20-22 DEG C during light management.

[0020] Further, the light management in spring or autumn is: the light intensity of the cultivation environment is set to 13000Lx-17000Lx in the early stage of planting; the light intensity is set to 33000Lx-37000Lx in the large seedling stage; the light intensity is set to 48000Lx-52000Lx in the stage from budding to bud coloration.

[0021] Further, the light management in summer is: the greenhouse can use shading net to shade in the early stage of planting, and all shading is used to reduce the environmental temperature; the light intensity of the cultivation environment is set to 0Lx-100Lx in the early stage of planting; the light intensity is set to 33000Lx-37000Lx in the large seedling stage; the light intensity is set to 48000Lx-52000Lx in the stage from budding to bud coloration.

[0022] Further, the light management in winter is: the light intensity of the cultivation environment in the initial planting stage is set to 33000Lx-37000Lx; the light intensity in the large seedling stage is set to 38000Lx-42000Lx, and the light intensity in the present bud stage to the bud coloration stage is set to 53000Lx-57000Lx.

[0023] Further, the application amount of the nitrogen-phosphorus-potassium compound fertilizer is 20kg / hm 2 -30kg / hm 2 , the application amount of the humic acid fertilizer is 9.0kg / hm 2 -10.0kg / hm 2 , the application amount of the seaweed fertilizer is 9.0kg / hm 2 -10.0kg / hm 2 , the application amount of the chelated iron fertilizer is 25g / hm 2 -35g / hm 2 , the application amount of the potassium dihydrogen phosphate is 115g / hm 2 -125g / hm 2 , the application amount of the chelated calcium fertilizer is 25g / hm 2 -35g / hm 2 .

[0024] The initial planting stage in the application is from planting the seed ball in the disinfected soil, and is usually 7 days-14 days; the seedling stage is 15 days-35 days after planting the seed ball; the large seedling stage is 35 days-45 days after planting the seed ball; the present bud stage is 45 days-60 days after planting the seed ball; and the coloration stage is 60 days-80 days after planting the seed ball.

[0025] Compared with the prior art, the application has the beneficial effects that:

[0026] The application defines the whole growth and development period of the double-flowered lily cultivation, including the accurate temperature and light conditions in different stages of the seed ball germination period, the initial planting stage, the present bud stage and the coloration stage, and simultaneously assists the corresponding water and fertilizer, soil disinfection comprehensive cultivation management method, significantly reduces the occurrence of the double-flowered lily petal reversion phenomenon, guarantees the high double-flowered characteristics of the double-flowered lily, improves the commodity traits and ornamental quality of the double-flowered lily, avoids the generation of defective flowers, significantly improves the ornamental value and commercial value, provides technical support for the high-quality and efficient cultivation of the new and excellent lily variety and the sustainable development of the lily industry, and has a wide application prospect. BRIEF DESCRIPTION OF DRAWINGS

[0027] In order to make the technical solutions in the embodiments of the present application or the prior art clearer, the accompanying drawings needed in the embodiments or the prior art description will be briefly introduced. Obviously, the accompanying drawings in the following description only show some embodiments of the present application, and other drawings can be obtained by those of ordinary skill in the art without any creative effort based on these drawings.

[0028] Figure 1 Fig. 1 is a schematic diagram of petal reversion, pistil and stamen reversion of a double-flowered lily; Fig. 1A shows that the pistil is incompletely petalized, the pistil is reappeared and the stigma is deformed; Fig. 1B shows that the stamen is incompletely petalized, the anther is reappeared and deformed; Fig. 1C shows the deformed anther attached to the edge of the petal; and Fig. 1D shows the deformed stigma of the pistil and the deformed anther.

[0029] Figure 2 Fig. 2 is a schematic diagram of a large number of pistil and stamen reversion in a cultivated population; Fig. 2A shows a large number of pistils and stamens without petalization; and Fig. 2B shows a comparison between a flower with petal reversion and a completely petalized flower. DETAILED DESCRIPTION

[0030] The specific embodiments of the present application will be described in detail below, but it should be understood that the protection scope of the present application is not limited by the specific embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without any creative effort belong to the protection scope of the present application. The experimental methods described in the embodiments of the present application are all conventional methods, and the materials and reagents used in the following embodiments can be obtained from commercial channels unless otherwise specified.

[0031] Embodiment 1: A method for preventing petal reversion of a double-flowered lily.

[0032] The present application takes the lily ‘Ice Beauty’ (Lilium candidum. Snow Beauty) as an example for experiment, including the following steps:

[0033] The lily ‘Ice Beauty’ bulb in this embodiment is purchased from Haining Huahai Gardening Co., Ltd., which is a double-flowered lily bulb that has been cold-treated and can normally bloom.

[0034] Preparation of materials: Select double-flowered lily bulbs that have been cold-treated and have no pests and diseases, and the lily bulb has a circumference of 15 cm. After summer high-temperature mulching for 55 days, the cow and sheep manure is fully decomposed.

[0035] Soil disinfection: The decomposed cow and sheep manure is mixed with the soil at a ratio of 600 kg / hm 2 Fertilization, as base fertilizer; deep ploughing of the soil to 30 cm, mixing the base fertilizer with the soil, and after land preparation, pouring the soil and covering it with plastic film. After continuous sunny day high-temperature mulching for 8 days for disinfection, opening the plastic film for ventilation for 5 days, and then using, the disinfected soil is obtained.

[0036] Low temperature germination: the heavy double tulip bulbs are transplanted in the bulb box with the mixture of grass carbon and perlite as the substrate, the volume ratio of which is 2:1, low temperature germination is carried out at 9℃, the water content of the substrate is ensured to be 60%, after 2 weeks, when the buds grow to 5cm, they can be planted in the sterilized soil.

[0037] Environmental temperature regulation: the environmental temperature is set to 15℃ from the bulb planting to the seedling period; the environmental temperature is set to 21℃ from the seedling period to the budding period; the environmental temperature is set to 25℃ during the coloration period.

[0038] Light management (spring and autumn seasons): the light intensity of the cultivation environment is set to 15000Lx during the initial planting period; the light intensity is set to 35000Lx when the plants grow to 8cm during the large seedling period; the light intensity is set to 50000Lx when the plants bud, and the light duration is ensured to be 10h, and the soil temperature is 22℃.

[0039] Water and fertilizer management: water and fertilizer integration is implemented, the bulbs are watered 3 times during planting; nitrogen, phosphorus and potassium compound fertilizer is applied every 12 days during the growth period, 25kg per mu is applied with water. After one month of planting, humic acid fertilizer and seaweed fertilizer are applied three times, the first time is 7.3kg per mu, the second and third times are 11.3kg per mu respectively; 30g per mu of chelated iron fertilizer (diluted 1500 times) is applied every 10 days for 2 times when the seedlings grow for 1.5 months; 1.5% of monobasic potassium phosphate aqueous solution is sprayed on the leaves 3 times when the plants bud, every 5 days, 120g per mu, diluted 300 times; from November to the following March, during the low temperature season, chelated calcium is sprayed on the leaves 3 times after the plants bud, every 7 days, 30g per mu, diluted 1000 times.

[0040] The nitrogen, phosphorus and potassium compound fertilizer is commercially available, the composition is N:P2O5:K2O=14:16:15, and it is purchased from Xinyangfeng Agricultural Science and Technology Co., Ltd.

[0041] The humic acid fertilizer is commercially available, the composition is humic acid ≥55%, potassium oxide ≥12%, and it is purchased from Hebei Nongxun Technology Co., Ltd.

[0042] The seaweed fertilizer is commercially available, the main raw material is deep sea horsetail, the effective components are seaweed acid ≥20%, organic matter ≥50%, N+P2O5+K2O ≥20%, and it is purchased from Weifang Wohaihong Biological Technology Co., Ltd.

[0043] The chelated iron fertilizer is commercially available, the main raw material is EDTA chelated iron, Fe ≥6%, and it is purchased from Jinzhou Tuotian Agricultural Co., Ltd.

[0044] The potassium dihydrogen phosphate is commercially available, and is purchased from Hubei Potash Chemical Co., Ltd.

[0045] The chelated calcium is commercially available, and the main raw material is EDTA chelated calcium, Ca≥9.6%. It is purchased from Zhengzhou Zixi Chemical Co., Ltd.

[0046] Netting: After the flower buds are colored, the colored flower buds are successively netted.

[0047] The seed ball initial stage in the embodiment starts from planting the seed ball in the disinfected soil, and is usually 7-14 days; the seedling stage is 15-35 days after planting the seed ball; the large seedling stage is 35-45 days after planting the seed ball; the budding stage is 45-60 days after planting the seed ball; and the coloring stage is 60-80 days after planting the seed ball.

[0048] Example 2: A method for preventing the petalization of heavy double lily petals.

[0049] In the embodiment, the raw material sources are the same as those in Example 1, and the experimental steps are basically the same, and the difference lies in that:

[0050] Soil disinfection: the fermented cow and sheep manure is 500 kg / hm 2 Fertilization; deep ploughing the soil to 30 cm, fully mixing with the soil, covering the plastic film on the top, and after the continuous sunny day disinfection for 7 days at high temperature, ventilating for 5 days after opening the plastic film, and then using, the disinfected soil is obtained.

[0051] Low-temperature germination: the seed ball is transplanted in the seed ball box with grass carbon and perlite as the substrate, and low-temperature germination is carried out at 10℃, the substrate moisture content is ensured to be 60%, and when the bud length is 5 cm, it can be planted in the disinfected soil. The substrate is a mixture of grass carbon and perlite in a volume ratio of 2:1.

[0052] Environmental temperature regulation: from planting the seed ball in the disinfected soil to the environmental temperature setting at 12℃ during the seedling period; the environmental temperature is increased to 20℃ from the seedling stage to the budding stage; and the environmental temperature is controlled at 26℃ after the flower bud is colored.

[0053] Illumination management (high temperature season in summer): shading net can be used for shading in the early stage of planting, and full shading is used to reduce the environmental temperature. The illumination intensity of the cultivation environment in the early stage of planting is 50Lx; the illumination intensity is set to 35000Lx when the plant grows to 8 cm in the large seedling stage; the illumination intensity is set to 50000Lx when the plant buds; and the illumination time is ensured to be 10h, and the soil temperature is 22℃.

[0054] Example 3: A method for preventing the petalization of heavy double lily petals.

[0055] The raw material sources in this embodiment are the same as those in Example 1, and the experimental steps are basically the same, the difference is that:

[0056] Soil disinfection: the fermented cow and sheep manure is 700 kg / hm 2 Fertilization; deep ploughing 30 cm, fully mixed with soil, covered with plastic film, after 9 days of high temperature in continuous sunny days, open the plastic film for ventilation for 5 days, and then use.

[0057] Low temperature germination: the seed ball is planted in a seed ball box with grass carbon and perlite as the substrate, low temperature germination is carried out at 8℃, the substrate moisture content is ensured to be 60%, and when the bud length is 5cm, it can be planted in the disinfected soil. The substrate is a mixture of grass carbon and perlite in a volume ratio of 2:1.

[0058] Environmental temperature regulation: from the beginning of planting the seed ball in the disinfected soil to the seedling period, the environmental temperature is set to 16℃; after germination, the environmental temperature is increased to 22℃; after the flower bud is colored, the environmental temperature is controlled at 25℃.

[0059] Light management (winter): the light intensity of the cultivation environment is set to 35000Lx in the early stage of planting; the light intensity is set to 40000Lx when the plant grows to 8cm in size; the light intensity is set to 55000Lx when the plant blooms; the light duration is ensured to be 10h, and the soil temperature is 20℃.

[0060] Example 1-3 precisely controls the whole growth and development period of double-flowered lily 'Ice Beauty', including the temperature conditions and light conditions in different stages of seed ball germination, early planting, budding and coloring, assisted by corresponding water and fertilizer, soil disinfection regulation, without the phenomenon of double-flowered lily petal reversion, avoiding the generation of defective flowers, significantly improving the ornamental value and commercial value. If 'Ice Beauty' is managed according to the conventional management of single-flowered lily, the defective flowers can reach 15%-30%. Therefore, the precise and effective environmental regulation of temperature and light is the key to prevent double-flowered lily petal reversion.

[0061] Although the preferred embodiments of the present application have been described, those skilled in the art can make further changes and modifications to these embodiments once they know the basic creative concept.

[0062] Obviously, those skilled in the art can make various modifications and changes to the present application without departing from the spirit and scope of the present application. Thus, if these modifications and changes of the present application belong to the scope of the claims of the present application and their equivalent technologies, the present application also intends to include these modifications and changes.

Claims

1. A method of preventing petal reversion in a double tulip, characterized in that, It comprises the following steps: Soil disinfection: fermented cattle and sheep manure is applied at 500 kg / hm 2 - 700 kg / hm 2 Fertilization, as base fertilizer, is mixed with soil and high-temperature smothering is used for soil disinfection; Low-temperature germination: placing the seeds in a substrate and carrying out low-temperature germination at 8-10°C, and when the sprouts grow to 5-6 cm, planting them in the sterilized soil; The substrate is a mixture of grass charcoal and perlite at a volume ratio of 1-3:1; Environmental temperature regulation: setting the environmental temperature at 12-16°C during the period from planting the seeds to germination, 18-22°C during the period from germination to budding, and 23-28°C during the coloration period of the flower buds; Illumination management: setting the illumination intensity at 0-37000Lx during the initial planting period, 33000-42000Lx during the large seedling period, and 48000-57000Lx during the period from budding to the coloration of the flower buds; Water and fertilizer management: watering thoroughly when planting the seeds, applying nitrogen, phosphorus and potassium compound fertilizer during the growth period, applying humic acid fertilizer and seaweed fertilizer one month after planting, applying chelated iron fertilizer one and a half months after germination, applying potassium dihydrogen phosphate solution when budding, and spraying chelated calcium fertilizer on the leaves when the air temperature is low in winter; Netting: wearing a net on the coloration flower buds.

2. The method for preventing petal reversion of a Crinum asiaticum according to claim 1, wherein The soil sterilization is carried out by covering a plastic film, sterilizing in a high-temperature greenhouse for 7-10 days, opening the plastic film for ventilation for 4-6 days, and then using the soil.

3. The method for preventing petal reversion in a Crinum hybrid as claimed in claim 1, wherein, The seeds have a circumference of 14-16 cm.

4. The method for preventing petal reversion in a Crinum hybrid as claimed in claim 1, wherein, The low-temperature germination substrate is a mixture of grass charcoal and perlite at a volume ratio of 2-3:

1.

5. The method for preventing petal reversion in a Crinum hybrid as claimed in claim 1, wherein, The illumination management is carried out for 8-12 hours.

6. The method for preventing petal reversion in a Crinum hybrid as claimed in claim 1, wherein, The soil temperature is maintained at 20-22°C during the illumination management.

7. The method for preventing petal reversion in a Crinum hybrid as claimed in claim 1, wherein, The illumination management in spring or autumn is carried out as follows: setting the illumination intensity at 13000-17000Lx during the initial planting period, 33000-37000Lx during the large seedling period, and 48000-52000Lx during the period from budding to the coloration of the flower buds.

8. The method for preventing petal reversion of a Crinum asiaticum according to claim 1, wherein The illumination management in summer is carried out as follows: shading the greenhouse with a shading net during the initial planting period to reduce the environmental temperature, setting the illumination intensity at 0-100Lx during the initial planting period, 33000-37000Lx during the large seedling period, and 48000-52000Lx during the period from budding to the coloration of the flower buds.

9. The method for preventing petal reversion of a Crinum asiaticum according to claim 1, wherein The illumination management in winter is carried out as follows: setting the illumination intensity at 33000-37000Lx during the initial planting period, 38000-42000Lx during the large seedling period, and 53000-57000Lx during the period from budding to the coloration of the flower buds.

10. The method for preventing petal reversion of a Crinum asiaticum according to claim 1, wherein The application amount of the nitrogen, phosphorus and potassium compound fertilizer is 20 kg / hm 2 -30 kg / hm 2 The application amount of the humic acid fertilizer is 9.0 kg / hm 2 -10.0 kg / hm 2 The application amount of the seaweed fertilizer is 9.0 kg / hm 2 -10.0 kg / hm 2 The application amount of the chelated iron fertilizer is 25 g / hm 2 -35 g / hm 2 The application amount of the potassium dihydrogen phosphate is 115 g / hm 2 -125 g / hm 2 The application amount of the chelated calcium fertilizer is 25 g / hm 2 -35 g / hm 2 .

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