Cold extraction method of chlorogenic acid in honeysuckle raw material

Abstract

The invention is applicable to the technical field of chlorogenic acid purification, and provides a method for cold extraction of chlorogenic acid in a honeysuckle raw material, which comprises the following steps: putting the honeysuckle raw material into an extraction tank, compacting the top, injecting a prepared sodium hydroxide solution into the extraction tank to completely immerse the material, and extracting for 2-4 hours at the top, after soaking for 24-48 hours, percolating according to the flow rate of 0.2-1L / min, and controlling the concentration of the sodium hydroxide solution to be 0.02%-0.15%; then the extraction tank is controlled to discharge liquid medicine into the storage tank at the same flow speed, the liquid medicine in the storage tank is pumped into the concentrator to be concentrated until the required density is 1.02-1.10, a concentrated solution is obtained, the concentrated solution is dried to obtain powder, the extraction temperature is low, components are not damaged, the extraction efficiency is high, the extraction cost is low, and the safety is good.

Description

Technical Field

[0001] The invention belongs to the technical field of chlorogenic acid generation and purification, and in particular relates to a cold extraction method of chlorogenic acid in a honeysuckle raw material. Background Art

[0002] Honeysuckle (also known as honeysuckle) is a perennial semi-evergreen shrub of the genus Honeysuckle in the family Caprifoliaceae. It is distributed in most parts of China and is also produced in Japan and Korea.

[0003] Honeysuckle has been famous for its medicinal value since ancient times. Its main effect is to clear away heat and detoxify, and it is mainly used to treat fever caused by febrile diseases, dysentery caused by heat toxins, carbuncle, furuncle, etc. Modern research has shown that honeysuckle contains pharmacologically active ingredients such as chlorogenic acid and luteolin glycosides, which have strong inhibitory effects on various pathogenic bacteria such as hemolytic streptococci and Staphylococcus aureus, as well as pathogenic viruses of upper respiratory tract infections.

[0004] A Chinese patent discloses a "process for extracting high-content chlorogenic acid by macroporous resin adsorption". Using honeysuckle as the raw material, after extraction and concentration, ethanol is added for alcohol precipitation, filtration, and the filtrate is concentrated. After one macroporous resin adsorption separation and elution, spray drying is performed to obtain a chlorogenic acid product with a chlorogenic acid purity of 40%; A Chinese patent discloses a "method for extracting and purifying chlorogenic acid from honeysuckle". Using honeysuckle as the raw material, after extraction and concentration, a flocculant is added for flocculation and precipitation, filtration, the filtrate is adsorbed and separated by a macroporous resin, and vacuum drying is performed to obtain a chlorogenic acid product with a yield of 22% and a purity of 48%.

[0005] There are some defects in the extraction of chlorogenic acid in the prior art, including high extraction temperature, easy destruction of effective ingredients, and complex process. Summary of the invention

[0006] The purpose of the embodiments of the present invention is to provide a method for cold extraction of chlorogenic acid from a raw material of honeysuckle, aiming to solve the problems existing in the prior art identified in the background technology.

[0007] The embodiment of the present invention is achieved by providing a method for cold extraction of chlorogenic acid from a raw material of honeysuckle, the method comprising the following steps:

[0008] Step S01: putting the honeysuckle raw material into an extraction tank and compacting the top, wherein the honeysuckle raw material includes honeysuckle;

[0009] Step S02: inject the prepared sodium hydroxide solution into the extraction tank to completely immerse the material, soak for 24-48 hours, and then percolate at a flow rate of 0.2-1 L / min. The concentration of the sodium hydroxide solution is 0.02%-0.15%;

[0010] Step S03: Control the extraction tank to release the liquid medicine into the storage tank at the same flow rate;

[0011] Step S04: the liquid medicine in the storage tank is pumped into a concentrator for concentration to a required density of 1.02-1.10 to obtain a concentrated liquid;

[0012] Step S05: drying the concentrated liquid to obtain powder.

[0013] Preferably, the extraction tank comprises a percolation tank.

[0014] Preferably, before putting the honeysuckle into the extraction tank, the method further comprises: laying a 3-4 cm layer of prismatic clean slag at the bottom of the extraction tank.

[0015] Preferably, after the honeysuckle is put into the extraction tank, its top is compacted with a porous stainless steel plate.

[0016] Preferably, the concentrate and powder can be used to prepare oral liquid and solid dosage forms, respectively.

[0017] Preferably, the honeysuckle raw material includes one or more of honeysuckle, honeysuckle leaves and honeysuckle vine.

[0018] Preferably, the concentration of the sodium hydroxide solution is 0.05%-0.08%.

[0019] Preferably, the flow rate of diafiltration is 0.5L-0.8L / min.

[0020] Preferably, when the concentration of sodium hydroxide solution is 0.05%-0.08%, the flow rate of diafiltration is 0.5L-0.8L / min.

[0021] The embodiment of the present invention provides a method for cold extraction of chlorogenic acid in a honeysuckle raw material. The method comprises: putting the honeysuckle raw material into an extraction tank and compacting the top. The honeysuckle raw material comprises honeysuckle, and injecting a prepared sodium hydroxide solution into the extraction tank to completely immerse the material. After soaking for 24-48 hours, percolating at a flow rate of 0.2-1L / min, and the concentration of the sodium hydroxide solution is 0.02%-0.15%; then controlling the extraction tank to release the drug solution into the storage tank at the same flow rate, and the drug solution in the storage tank is pumped into a concentrator to be concentrated to the required density of 1.02-1.10 to obtain a concentrated solution, and drying the concentrated solution to obtain a powder. The extraction temperature is low, the components are not destroyed, the extraction efficiency is high, the extraction cost is low, and the safety is good. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 The present invention provides a flowchart of a method for cold extraction of chlorogenic acid from a raw material of honeysuckle. DETAILED DESCRIPTION

[0023] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0024] The specific implementation of the present invention is described in detail below in conjunction with specific embodiments.

[0025] like Figure 1 As shown, it is a structural diagram of a method for cold extraction of chlorogenic acid from a honeysuckle raw material provided by one embodiment of the present invention.

[0026] Example 1

[0027] Ingredients: Fresh honeysuckle, fresh honeysuckle leaves, fresh honeysuckle vines

[0028] Cold extraction (percolation): Key parameters:

[0029] Sodium hydroxide concentration: 0.02%-0.15%, total material-liquid ratio: 1:20

[0030] Flow rate: 0.2-1L / min;

[0031] 1.1 Before feeding, lay a layer of 3-4 cm prismatic clean slag at the bottom of the percolation tank (or take other measures to prevent clogging of the filter screen), put the honeysuckle into the tank, and compact it with a porous stainless steel plate (to prevent the honeysuckle from floating);

[0032] 1.2 Inject the prepared sodium hydroxide solution (0.02%-0.15%) into the material tank to completely immerse the material. After soaking for 24-48 hours, percolate at a flow rate of 0.2-1L / min.

[0033] 1.3 The percolation tank discharges the liquid medicine into the storage tank at the same flow rate.

[0034] 1.4 The liquid in the storage tank is pumped into the concentrator to be concentrated to the required density (1.02-1.10). The chlorogenic acid content is 15-50 mg / ml.

[0035] 1.5 The concentrated liquid is dried to obtain powder.

[0036] Example 2

[0037] Ingredients: dried honeysuckle, dried honeysuckle leaves, dried honeysuckle vines

[0038] Cold extraction (percolation): Key parameters:

[0039] Sodium hydroxide concentration: 0.02%-0.15%, total material-liquid ratio: 1:20

[0040] Flow rate: 0.2-1L / min

[0041] 1.1 Before feeding, lay a layer of 3-4 cm prismatic clean slag at the bottom of the percolation tank (or take other measures to prevent clogging of the filter screen), put the honeysuckle into the tank, and compact it with a porous stainless steel plate (to prevent the honeysuckle from floating);

[0042] 1.2 Inject the prepared sodium hydroxide solution (0.02%-0.15%) into the material tank to completely immerse the material. After soaking for 24-48 hours, percolate at a flow rate of 0.2-1L / min.

[0043] 1.3 The percolation tank discharges the liquid medicine into the storage tank at the same flow rate.

[0044] 1.4 The liquid in the storage tank is pumped into the concentrator to be concentrated to the required density (1.02-1.10). The chlorogenic acid content is 15-48 mg / ml.

[0045] 1.5 The concentrated liquid is dried to obtain powder.

[0046] The concentrated liquid and powder can be used to prepare oral liquid and solid dosage form.

[0047] Example 3

[0048] Ingredients: Fresh honeysuckle, fresh honeysuckle leaves, fresh honeysuckle vines

[0049] Cold extraction (percolation): Key parameters:

[0050] Sodium hydroxide concentration: 0.02%-0.15%, total material-liquid ratio: 1:20;

[0051] Flow rate: 0.2-1L / min;

[0052] 1.1 Before feeding, lay a layer of 3-4 cm prismatic clean slag at the bottom of the percolation tank (or take other measures to prevent clogging of the filter screen), put the honeysuckle into the tank, and compact it with a porous stainless steel plate (to prevent the honeysuckle from floating);

[0053] 1.2 Inject the prepared sodium hydroxide solution (0.05%-0.08%) into the material tank to completely immerse the material. After soaking for 24-48 hours, percolate at a flow rate of 0.2-1L / min.

[0054] 1.3 The percolation tank discharges the liquid medicine into the storage tank at the same flow rate.

[0055] 1.4 The liquid in the storage tank is pumped into the concentrator to be concentrated to the required density (1.02-1.10). The chlorogenic acid content is 16-53 mg / ml.

[0056] 1.5 The concentrated liquid is dried to obtain powder;

[0057] The concentrated liquid and powder can be used to prepare oral liquid and solid dosage form.

[0058] Example 3

[0059] Ingredients: Fresh honeysuckle, fresh honeysuckle leaves, fresh honeysuckle vines

[0060] Cold extraction (percolation): Key parameters:

[0061] Sodium hydroxide concentration: 0.02%-0.15%, total material-liquid ratio: 1:20;

[0062] Flow rate: 0.2-1L / min;

[0063] 1.1 Before feeding, lay a layer of 3-4 cm prismatic clean slag at the bottom of the percolation tank (or take other measures to prevent clogging of the filter screen), put the honeysuckle into the tank, and compact it with a porous stainless steel plate (to prevent the honeysuckle from floating);

[0064] 1.2 Inject the prepared sodium hydroxide solution (0.05%-0.08%) into the material tank to completely immerse the material. After soaking for 24-48 hours, percolate at a flow rate of 0.2-1L / min.

[0065] 1.3 The percolation tank discharges the liquid medicine into the storage tank at the same flow rate.

[0066] 1.4 The liquid in the storage tank is pumped into the concentrator to be concentrated to the required density (1.02-1.10). The chlorogenic acid content is 15-52 mg / ml.

[0067] 1.5 The concentrated liquid is dried to obtain powder;

[0068] The concentrated liquid and powder can be used to prepare oral liquid and solid dosage form.

[0069] Example 4

[0070] Ingredients: Fresh honeysuckle, fresh honeysuckle leaves, fresh honeysuckle vines

[0071] Cold extraction (percolation): Key parameters:

[0072] Sodium hydroxide concentration: 0.02%-0.15%, total material-liquid ratio: 1:20;

[0073] Flow rate: 0.2-1L / min;

[0074] 1.1 Before feeding, lay a layer of 3-4 cm prismatic clean slag at the bottom of the percolation tank (or take other measures to prevent clogging of the filter screen), put the honeysuckle into the tank, and compact it with a porous stainless steel plate (to prevent the honeysuckle from floating);

[0075] 1.2 Inject the prepared sodium hydroxide solution (0.05%-0.08%) into the material tank to completely immerse the material. After soaking for 24-48 hours, percolate at a flow rate of 0.5L-0.8L / min.

[0076] 1.3 The percolation tank discharges the liquid medicine into the storage tank at the same flow rate.

[0077] 1.4 The liquid in the storage tank is pumped into the concentrator to be concentrated to the required density (1.02-1.10). The chlorogenic acid content is 15-60 mg / ml.

[0078] 1.5 The concentrated liquid is dried to obtain powder;

[0079] The concentrated liquid and powder can be used to prepare oral liquid and solid dosage form.

[0080] The solvent in the above embodiments is food grade sodium hydroxide.

[0081] The above embodiments of the present invention provide a method for cold extraction of chlorogenic acid from a honeysuckle raw material, which has low extraction temperature, does not destroy the components, has high extraction efficiency, low extraction cost and good safety.

[0082] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for cold extraction of chlorogenic acid from honeysuckle raw materials, It is characterized in that The method comprises: Step S01: putting the honeysuckle raw material into an extraction tank and compacting the top, wherein the honeysuckle raw material includes honeysuckle; Step S02: inject the prepared sodium hydroxide solution into the extraction tank to completely immerse the material, soak for 24-48 hours, and then percolate at a flow rate of 0.2-1 L / min. The concentration of the sodium hydroxide solution is 0.02%-0.15%; Step S03: Control the extraction tank to release the liquid medicine into the storage tank at the same flow rate; Step S04: the liquid medicine in the storage tank is pumped into a concentrator for concentration to a required density of 1.02-1.10 to obtain a concentrated liquid; Step S05: drying the concentrated liquid to obtain powder.

2. The cold extraction method of chlorogenic acid in the raw material of honeysuckle according to claim 1, It is characterized in that The extraction tank comprises a percolation tank.

3. The cold extraction method of chlorogenic acid in the raw material of honeysuckle according to claim 1, It is characterized in that Before putting the honeysuckle into the extraction tank, the method further comprises: laying a layer of 3-4 cm prismatic clean slag stones on the bottom of the extraction tank.

4. The cold extraction method of chlorogenic acid in the raw material of honeysuckle according to claim 1, It is characterized in that After the honeysuckle is put into the extraction tank, its top is compacted with a porous stainless steel plate.

5. The cold extraction method of chlorogenic acid in the raw material of honeysuckle according to claim 2, It is characterized in that The concentrate and powder can be used to prepare oral liquid and solid dosage forms respectively.

6. The cold extraction method of chlorogenic acid in the raw material of honeysuckle according to claim 1, It is characterized in that The honeysuckle raw material includes one or more of honeysuckle, honeysuckle leaves and honeysuckle vines.

7. The cold extraction method of chlorogenic acid in the raw material of honeysuckle according to claim 1, It is characterized in that The concentration of the sodium hydroxide solution is 0.05%-0.08%.

8. The cold extraction method of chlorogenic acid in the raw material of honeysuckle according to claim 1, It is characterized in that The flow rate of percolation is 0.5L-0.8L / min.

9. The cold extraction method of chlorogenic acid from the raw material of honeysuckle according to any one of claims 1 to 8, It is characterized in that When the concentration of sodium hydroxide solution is 0.05%-0.08%, the flow rate of diafiltration is 0.5L-0.8L / min.

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