Application of traditional Chinese medicine composition in preparation of medicine for preventing and treating virus infectious diarrhea
Through the preparation of traditional Chinese medicine compositions, the problem that existing drugs have no significant effect on viral diarrhea treatment has been solved. The use of traditional Chinese medicine compositions such as Magnolia officinalis significantly improves symptoms such as abdominal pain, diarrhea, vomiting, and has a high degree of infectious diarrhea for rotavirus. Anti-infection effect promotes weight gain, reduces fecal index, and improves treatment effect.
Patent Information
- Application Number
- CN202410548365.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2023-06-14
- Filing Date
- 2024-05-06
- Publication Date
- 2024-12-17
AI Technical Summary
The existing therapeutic drugs have no significant effect on viral diarrhea, especially the incidence of viral gastroenteritis has increased year by year, and it is difficult for existing drugs to effectively prevent and treat them.
Using traditional Chinese medicine compositions, including Magnolia officinalis, roasted betel nuts, simmered berry fruits, ephedra, bitter almonds, ginger, patchouli, perilla, atractylodes, poria cocos, atractylodes, gypsum, scorched hawthorn, scorched lithogonum, scorched malt, The combination of Chinese medicinal materials such as Long, Xu Changqing and Pinezi is prepared into drugs through reflux extraction, filtration, concentration, centrifugation and drying, which is used to treat and prevent viral infectious diarrhea.
It significantly improves symptoms such as abdominal pain, diarrhea, and vomiting. It has anti-dirty diarrhea effect in rotavirus-infected lactation mice, promotes weight gain, reduces the fecal index of lactation mice, reduces the degree of diarrhea, and improves the treatment effect.
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Abstract
Description
[0001] This application claims the priority of the Chinese patent application filed with the China Patent Office on June 14, 2023, with application number 202310705589.4 and invention name "Application of Chinese herbal medicine composition in the preparation of drugs for preventing and treating viral infectious diarrhea", the entire contents of which are incorporated by reference into this application. Technical Field
[0002] The present invention relates to the technical field of traditional Chinese medicines, and in particular to application of a traditional Chinese medicine composition in preparing medicines for preventing and treating viral infectious diarrhea. Background Art
[0003] Viral diarrhea, also known as viral gastroenteritis, is an acute gastrointestinal infectious disease caused by intestinal viral infection. The clinical characteristics are acute onset, nausea, vomiting, abdominal pain, diarrhea (watery stool or loose stool), and may also have fever and general discomfort. In recent years, the incidence of viral diarrhea has increased year by year, especially in the autumn and winter seasons, when acute gastroenteritis caused by viral infection is much higher than acute gastroenteritis caused by bacteria. It can occur in all age groups, and is clinically accompanied by poisoning symptoms such as fever, nausea, and anorexia. There are many viruses that can cause this disease, the most common of which are rotavirus, norovirus, and enteric adenovirus. In addition, other viruses that can cause viral diarrhea include astrovirus, coxsackievirus, and coronavirus.
[0004] The existing therapeutic drugs are not effective. Therefore, providing a related product developed based on Chinese herbal medicine has important practical significance in the prevention and treatment of animal diarrhea. Summary of the invention
[0005] In view of this, the present invention provides the use of a Chinese medicine composition in the preparation of a drug for preventing and treating viral infectious diarrhea. The present invention has found through experiments that the Chinese medicine composition of the present invention has a certain therapeutic effect for treating viral diarrhea, significantly improves symptoms such as abdominal pain, diarrhea, vomiting, and has an anti-rotavirus-infected suckling mouse diarrhea effect, can improve the signs of infected suckling mice, promote weight gain, reduce the fecal index of suckling mice, and reduce the degree of diarrhea. The Chinese medicine composition can significantly improve the therapeutic effect, and the relief rate of abdominal pain and diarrhea symptoms of patients is also significantly improved, which has clinical promotion value.
[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:
[0007] The present invention provides the use of a traditional Chinese medicine composition in preparing a medicine for treating and / or preventing viral infectious diarrhea;
[0008] The Chinese medicine composition comprises: 1-100 parts of Magnolia officinalis, 1-100 parts of burnt Areca catechu, 1-100 parts of stewed Tsaoko fruit, 1-100 parts of Ephedra, 1-100 parts of Bitter Apricot, 1-100 parts of Notopterygium wilfordii, 1-100 parts of Ginger, 1-100 parts of Patchouli, 1-100 parts of Cyperus rotundus, 1-100 parts of Atractylodes macrocephala, 1-160 parts of Poria, 1-120 parts of Atractylodes macrocephala, 1-100 parts of Gypsum, 1-100 parts of burnt Crataegus pinnatifida, 1-100 parts of burnt Liu Shenqu, 1-100 parts of burnt malt, 1-100 parts of Pheretima, 1-100 parts of Cynanchum cyrtonema, 1-100 parts of Cynomorium wilfordii and / or 1-100 parts of Lepidium chinense.
[0009] In some specific embodiments of the present invention, the treatment or prevention of viral infectious diarrhea includes any of the following:
[0010] (I) Improve abdominal pain; and / or
[0011] (II), improve diarrhea; and / or
[0012] (III) Improve vomiting; and / or
[0013] (IV) Improve animal physical signs.
[0014] In some specific embodiments of the present invention, the improvement of animal physical signs includes any of the following:
[0015] (I) Improve body mass; and / or
[0016] (II) Reduce fecal index.
[0017] In some embodiments of the present invention, improving body mass includes promoting weight gain.
[0018] In some embodiments of the invention, the virus comprises a rotavirus.
[0019] In some specific embodiments of the present invention, the traditional Chinese medicine composition includes: 10-80 parts of Magnolia officinalis, 10-80 parts of charred Areca nut, 10-80 parts of stewed grass fruit, 10-60 parts of Ephedra, 10-60 parts of bitter almond, 10-80 parts of Notopterygium, 10-60 parts of ginger, 10-80 parts of Patchouli, 10-60 parts of Cyperus rotundus, 10-80 parts of Atractylodes, 10-160 parts of Poria, 10-120 parts of Atractylodes, 10-80 parts of Gypsum, 10-50 parts of charred Crataegus pinnatifida, 10-80 parts of charred Liu Shenqu, 10-60 parts of charred malt, 10-80 parts of Pheretima, 10-80 parts of Cynomorium wilfordii, 10-60 parts of Cyperus rotundus and / or 10-80 parts of Lemongrass.
[0020] In some specific embodiments of the present invention, the Chinese medicine composition comprises: 30-50 parts of Magnolia officinalis, 20-30 parts of charred Areca nut, 30-50 parts of stewed grass fruit, 20-30 parts of Ephedra, 20-30 parts of Bitter Apricot, 30-50 parts of Notopterygium, 30-50 parts of Ginger, 30-50 parts of Patchouli, 20-30 parts of Perilla frutescens, 30-50 parts of Atractylodes, 120-150 parts of Poria, 80-100 parts of Atractylodes macrocephala, 30-50 parts of Gypsum, 20-30 parts of charred Crataegus pinnatifida, 30-50 parts of charred Liu Shenqu, 20-30 parts of charred malt, 30-50 parts of Pheretima, 30-50 parts of Cynanchum cyrtonema, 20-30 parts of Cynomorium wilfordii, and 30-50 parts of Lepidium chinense;
[0021] Preferentially, one or a combination of two or more of 50 parts of Magnolia officinalis, 30 parts of scorched Areca nut, 30 parts of stewed Tsaoko fruit, 20 parts of Ephedra, 30 parts of Bitter Apricot, 50 parts of Notopterygium wilfordii, 50 parts of Ginger, 50 parts of Patchouli, 30 parts of Perilla frutescens, 50 parts of Atractylodes macrocephala, 150 parts of Poria, 100 parts of Atractylodes macrocephala, 50 parts of Gypsum, 30 parts of scorched Crataegus pinnatifida, 30 parts of scorched Liu Shenqu, 30 parts of scorched malt, 50 parts of Pheretima, 50 parts of Cynanchum chinense, 30 parts of Cynomorium mongolicum and / or 50 parts of Semen Lepidium.
[0022] In some specific embodiments of the present invention, the Chinese medicine composition includes but is not limited to raw materials, powders, slices or other forms made from raw materials, and extracts, fermentation broths or other forms with the same or similar biological activities.
[0023] In some specific embodiments of the present invention, the preparation method of the traditional Chinese medicine composition comprises: taking a formula amount of one or a combination of two or more of Magnolia officinalis, charred Areca nut, stewed grass fruit, ephedra, bitter almond, Notopterygium wilfordii, ginger, patchouli, Perilla frutescens, Atractylodes lancea, Poria cocos, Atractylodes macrocephala, gypsum, charred hawthorn, charred Liushenqu, charred malt, earthworm, Cynanchum chinense, Mianma Guanzhong and / or Lemongrass seed, reflux extraction, filtering, concentrating, centrifuging, drying, and crushing to obtain an intermediate composition;
[0024] Taking the intermediate composition and mixing it with pharmaceutically acceptable excipients to prepare the Chinese medicine composition;
[0025] The reflux extraction includes: adding 6 times of water for the first time and extracting for 1.5 hours, and adding 4 times of water for the second time and extracting for 1.0 hour.
[0026] In some specific embodiments of the present invention, the acceptable excipients include one or more of a filler, a disintegrant, a lubricant, a suspending agent, a binder, a sweetener, a flavoring agent, a preservative or a matrix.
[0027] In some specific embodiments of the present invention, the preparation method of the traditional Chinese medicine composition comprises: taking 50 parts of Magnolia officinalis, 30 parts of burnt betel nut, 30 parts of stewed tsaoko, 20 parts of ephedra, 30 parts of bitter almond, 50 parts of notopterygium, 50 parts of ginger, 50 parts of patchouli, 30 parts of perilla, 50 parts of atractylodes, 150 parts of tuckahoe, 100 parts of atractylodes, 50 parts of gypsum, 30 parts of burnt hawthorn, 30 parts of burnt six Shenqu, 30 parts of burnt malt, 50 parts of earthworm, 50 parts of Cynanchum chinense, 30 parts of Cynomorium mongolicum, and 50 parts of Lemongrass, adding water for reflux extraction twice, combining the extracts, filtering, concentrating the filtrate to a relative density of 1.10-1.15, centrifuging, vacuum drying the filtrate, spray drying and pulverizing to obtain an intermediate composition, adding sucralose and dextrin, mixing, and obtaining the traditional Chinese medicine composition;
[0028] The reflux extraction includes: adding 6 times of water for the first time and extracting for 1.5 hours, and adding 4 times of water for the second time and extracting for 1.0 hour.
[0029] In some specific embodiments of the present invention, the dosage form of the drug includes: one or more of decoction, granules, capsules, tablets, oral liquid, pills, tincture, syrup, gel, spray or injection.
[0030] In some specific embodiments of the present invention, the effective dose of the drug includes 9.36-37.44 g crude drug / kg mouse body weight.
[0031] In some specific embodiments of the present invention, the administration of the drug includes one or more of oral administration, injection or external application.
[0032] The present invention provides the following beneficial effects including but not limited to:
[0033] The traditional Chinese medicine composition proposed by the present invention has a certain therapeutic effect on treating viral diarrhea, significantly improves symptoms such as abdominal pain, diarrhea, vomiting, etc., has an anti-rotavirus-infected suckling mouse diarrhea effect, can improve the physical signs of infected suckling mice, promote weight gain, reduce the fecal index of suckling mice, and alleviate the degree of diarrhea. DETAILED DESCRIPTION
[0034] The present invention discloses the use of a Chinese medicine composition in the preparation of a drug for preventing and treating viral infectious diarrhea. Those skilled in the art can refer to the content of this article and appropriately improve the process parameters to achieve it. It should be particularly noted that all similar substitutions and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the methods and applications described herein without departing from the content, spirit and scope of the present invention to implement and apply the technology of the present invention.
[0035] The present invention provides an application of a Chinese medicine composition in preparing a drug for treating or preventing viral infectious diarrhea, the Chinese medicine composition comprising: 1-100 parts of Magnolia officinalis, 1-100 parts of charred Areca nut, 1-100 parts of simmered Tsaoko fruit, 1-100 parts of Ephedra, 1-100 parts of Bitter Apricot, 1-100 parts of Notopterygium wilfordii, 1-100 parts of Ginger, 1-100 parts of Patchouli, 1-100 parts of Perilla frutescens, 1-100 parts of Atractylodes macrocephala, 1-160 parts of Poria cocos, 1-120 parts of Atractylodes macrocephala, 1-100 parts of Gypsum, 1-100 parts of charred Crataegus pinnatifida, 1-100 parts of charred Liu Shenqu, 1-100 parts of charred Malt, 1-100 parts of Pheretima, 1-100 parts of Cynanchum cyrtonema, 1-100 parts of Cynanchum cyrtonema, and 1-100 parts of Lepidium chinense. The application may be any method that is beneficial to improving the corresponding symptoms of the patient, including treatment or prevention.
[0036] Furthermore, the Chinese medicine composition includes: 10-80 parts of Magnolia officinalis, 10-80 parts of scorched Areca catechu, 10-80 parts of stewed Tsaoko fruit, 10-60 parts of Ephedra sinica, 10-60 parts of Bitter Apricot, 10-80 parts of Notopterygium wilfordii, 10-60 parts of Ginger, 10-80 parts of Patchouli, 10-60 parts of Cyperus rotundus, 10-80 parts of Atractylodes macrocephala, 10-160 parts of Poria cocos, 10-120 parts of Atractylodes macrocephala, 10-80 parts of Gypsum, 10-50 parts of scorched Crataegus pinnatifida, 10-80 parts of scorched Liu Shenqu, 10-60 parts of scorched malt, 10-80 parts of Pheretima, 10-80 parts of Cynanchum cyrtonema, 10-60 parts of Lemongrass seeds.
[0037] Preferably, the Chinese medicine composition comprises: 30-50 parts of Magnolia officinalis, 20-30 parts of charred Areca nut, 30-50 parts of stewed Tsaoko fruit, 20-30 parts of Ephedra, 20-30 parts of Bitter Apricot, 30-50 parts of Notopterygium wilfordii, 30-50 parts of Ginger, 30-50 parts of Patchouli, 20-30 parts of Perilla frutescens, 30-50 parts of Atractylodes lancea, 120-150 parts of Poria, 80-100 parts of Atractylodes macrocephala, 30-50 parts of Gypsum, 20-30 parts of charred Crataegus pinnatifida, 30-50 parts of charred Liu Shenqu, 20-30 parts of charred Malt, 30-50 parts of Pheretima, 30-50 parts of Cynanchum cyrtonema, 20-30 parts of Cynomorium wilfordii, and 30-50 parts of Lepidium chinense;
[0038] Preferentially, 50 parts of Magnolia officinalis, 30 parts of scorched areca nut, 30 parts of stewed tsaoko fruit, 20 parts of ephedra, 30 parts of bitter almond, 50 parts of notopterygium, 50 parts of ginger, 50 parts of patchouli, 30 parts of eupatorium, 50 parts of atractylodes, 150 parts of Poria, 100 parts of Atractylodes, 50 parts of gypsum, 30 parts of scorched hawthorn, 30 parts of scorched six Shenqu, 30 parts of scorched malt, 50 parts of earthworms, 50 parts of Cynanchum chinense, 30 parts of Cynomorium tuberosum, and 50 parts of Lemongrass seeds.
[0039] The above-mentioned Chinese medicine composition can be directly ground into powder by raw materials, or can be an extract or other forms prepared by conventional means.
[0040] Specifically, the dosage forms of the aforementioned viral infectious diarrhea drugs include oral dosage forms, injection dosage forms or external dosage forms.
[0041] The drug of the present invention is prepared by weighing the raw material drug in proportion, adding pharmaceutically acceptable excipients such as fillers, disintegrants, lubricants, suspending agents, adhesives, sweeteners, flavoring agents, preservatives, bases, etc., and preparing according to conventional production methods in the pharmaceutical field to prepare conventional dosage forms acceptable to pharmacy, including but not limited to decoctions, granules, capsules, tablets, oral liquids, pills, tinctures, syrups, suppositories, gels, sprays, and injections.
[0042] The present invention also provides a method for preparing any of the aforementioned Chinese medicine compositions, the method comprising:
[0043] Weigh Magnolia officinalis, scorched areca nut, stewed tsaoko, ephedra, bitter almond, notopterygium wilfordii, ginger, patchouli, perilla, atractylodes, Poria, Atractylodes macrocephala, gypsum, scorched hawthorn, scorched Liushenqu, scorched malt, earthworm, Cynanchum chinense, Cynomorium morganianum, and Semen tiliaceum, extract twice with water, add 6 times the amount of water for the first time and extract for 1.5 h, add 4 times the amount of water for the second time and extract for 1.0 h, combine the extracts, filter, concentrate the filtrate to a relative density of 1.10-1.15, centrifuge and filter, and dry the filtrate.
[0044] Specifically, the above preparation method has the following steps:
[0045] Take 50 parts of Magnolia officinalis, 30 parts of charred Areca nut, 30 parts of stewed Tsaoko fruit, 20 parts of Ephedra, 30 parts of Bitter Apricot, 50 parts of Notopterygium, 50 parts of Ginger, 50 parts of Patchouli, 30 parts of Perilla frutescens, 50 parts of Atractylodes, 150 parts of Poria, 100 parts of Atractylodes, 50 parts of Gypsum, 30 parts of charred Crataegus, 30 parts of charred Liu Shenqu, 30 parts of charred malt, 50 parts of Pheretima, 50 parts of Cynanchum indica, 30 parts of Cynomorium mongolicum, and 50 parts of Semen Lepidii, add water and reflux and extract twice, add 6 times of water for the first time, extract for 1.5h, add 4 times of water for the second time, extract for 1.0h, combine the extracts, filter, concentrate the filtrate to a relative density of 1.10-1.15, centrifuge and filter, vacuum dry the filtrate, spray dry and crush to obtain an intermediate composition, add sucralose and dextrin, mix well, and obtain granules.
[0046] The traditional Chinese medicine composition proposed by the present invention has a certain therapeutic effect on treating viral diarrhea, significantly improves symptoms such as abdominal pain, diarrhea, vomiting, etc., has an anti-rotavirus-infected suckling mouse diarrhea effect, improves the physical signs of infected suckling mice, promotes weight gain, reduces the fecal index of suckling mice, and alleviates the degree of diarrhea.
[0047] The following experiments, if no specific conditions are specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The APIs or excipients, reagents or instruments used, if the manufacturer is not specified, are all conventional products that can be purchased commercially. Unless otherwise specified, all percentages, ratios, proportions or parts are by weight.
[0048] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein can be applied to the present invention.
[0049] Unless otherwise specified, the raw materials and reagents used in the application of the Chinese medicine composition provided by the present invention in the preparation of drugs for preventing and treating viral infectious diarrhea can all be purchased from the market.
[0050] The present invention will be further described below in conjunction with embodiments:
[0051] Preparation of Chinese medicine composition granules
[0052] The Chinese medicine composition in this preparation example is made of the following raw materials: 50 grams of Magnolia officinalis, 30 grams of scorched betel nut, 30 grams of stewed grass fruit, 20 grams of ephedra, 30 grams of bitter almond, 50 grams of Notopterygium wilfordii, 50 grams of ginger, 50 grams of patchouli, 30 grams of Perilla frutescens, 50 grams of Atractylodes lancea, 150 grams of Poria, 100 grams of Atractylodes macrocephala, 50 grams of gypsum, 30 grams of scorched hawthorn, 30 grams of scorched Liu Shenqu, 30 grams of scorched malt, 50 grams of earthworm, 50 grams of Cynanchum chinense, 30 grams of Cynomorium fortunei, and 50 grams of Lemongrass.
[0053] The Chinese medicine composition is prepared according to the following method:
[0054] Take the above medicinal materials, add water and reflux extract twice, add 6 times water for the first time, extract for 1.5 hours, add 4 times water for the second time, extract for 1.0 hours, combine the extracts, filter, concentrate the filtrate to a relative density of 1.10-1.15, centrifuge and filter, vacuum dry the filtrate, spray dry and crush to obtain an intermediate composition, add sucralose and dextrin, mix well, and obtain the Chinese medicine composition granules.
[0055] Effect of Chinese medicine composition on diarrhea model of suckling mice infected with rotavirus
[0056] Experimental Materials
[0057] Animals: ICR mice, SPF grade, 40 males and females, purchased from the Comparative Medicine Center of Yangzhou University. In the experiment, male and female mice were mated together, and 7-day-old ICR suckling mice were used for the experiment.
[0058] Drug: Ribavirin tablets, Jiangxi Huiren Pharmaceutical Co., Ltd., batch number: 1312030; specification: 100 mg / box.
[0059] Test drug: the Chinese medicine composition granules prepared in the preparation example, provided by Jiangsu Kangyuan Pharmaceutical Co., Ltd. Drug dosage: The daily dosage of crude drugs for humans is 288 g, and the clinical dosage for adults (calculated as 70 kg) is 4.1 g crude drugs / kg. The equivalent dosage for mice is calculated to be 37.44 g crude drugs / kg. This dosage is set as the high dosage, and the low, medium, and high dosage groups are set according to the dosage ratio of 0.25:0.5:1, with dosages of 9.36, 18.72, and 37.44 g crude drugs / kg, respectively, which are equivalent to 2.3, 4.6, and 9.1 times the clinical dosage, respectively.
[0060] Reagents: Fetal bovine serum, FBS, GIBCO, specification: 500 mL / bottle, batch number: 10099-141; high-glucose DMEM culture medium, GIBCO, specification: 500 mL / bottle, batch number: 11995-065; trypsin cell digestion solution, Sangon Biotechnology, 100 mL / bottle, batch number: E607002-0100; PBS tablets, Amresco, USA, specification: 200 Tablets / bottle, batch number: E404-200TABS; Group A rotavirus detection kit, Wantai Bio-Pharmaceutical Co., Ltd., specification: 50 T / box, batch number: 20181209E.
[0061] Instruments: Thermo Scientific BB15 CO2 cell culture incubator, Thermo Electron Corporation, USA; TS100 inverted microscope, Nikon, Japan; A10051560 clean bench, AIRTECH; Varioskan Flash full-wavelength multifunctional microplate reader, Thermo Electron Corporation, USA; JA503 analytical electronic balance, Changzhou Lucky Electronic Equipment Co., Ltd.
[0062] Experimental methods
[0063] The 7-day-old suckling mice were divided into normal group, model group, different dose groups of the Chinese medicine composition granules of the present invention (9.36g crude drug / kg, 18.72g crude drug / kg, 37.44g crude drug / kg) and ribavirin positive drug group (0.1g / kg) according to the litter, with 10 mice in each group. The normal group was gavaged with 100 μL / mouse of DMEM culture medium; the model group and the drug group were gavaged with SA-11 strain RV fluid (100 TCID50, 100 times the half tissue culture infection dose of the virus), 100 μL / mouse, and the positive result of the group A rotavirus detection kit indicated that the model was successfully established. After 24 hours, the ribavirin group and the different dose groups of the Chinese medicine composition granules of the present invention were gavaged with 100 μL, and the drug concentration was calculated according to the weight of the suckling mice before each gavage; the normal group and the model group were gavaged with double distilled water once a day for 5 consecutive days.
[0064] Detection indicators: ① Record the weight of each suckling mouse every day for 5 consecutive days. ② Press the abdomen of each suckling mouse lightly to observe the feces it excretes, score the degree of diarrhea based on the color and shape of the feces, and record the diarrhea score, which is divided into 0 to 4 points. 0 point: no feces; 1 point: normal feces; 2 points: light yellow soft feces; 3 points: light yellow loose feces; 4 points: watery feces. The judgment of the diarrhea score is completed by the same person.
[0065] The results of weight changes in suckling mice showed that on the second day of rotavirus infection, that is, the first day of administration, the model group lost weight compared with the normal control group (P < 0.01); on the 3rd to 6th day of modeling, that is, the 2nd to 5th day of administration, the weight gain was slower than that of the normal group (P < 0.001). Compared with the model group, on the second day of administration, the ribavirin group (P < 0.001) and the high-dose group of the Chinese medicine composition of the present invention (P < 0.01) were significantly different, and there was no significant difference between the medium and low-dose groups of the Chinese medicine composition of the present invention. Compared with the model group, on the third day of administration, there were significant differences in the ribavirin group (P < 0.001), the high-dose group (P < 0.001), and the medium-dose group (P < 0.01), and there was no significant difference in the low-dose group. Compared with the model group, after 4 days of administration, there were significant differences in the ribavirin group (P < 0.001), high-dose group (P < 0.001), medium-dose group (P < 0.001) and low-dose group (P < 0.05). Compared with the model group, after 5 days of administration, there were significant differences in the ribavirin group (P < 0.001), high-dose group (P < 0.001), medium-dose group (P < 0.001) and low-dose group (P < 0.01). The results are shown in Table 1.
[0066] The results of diarrhea scores of each group of suckling mice showed that compared with the normal group, the fecal index of the model group was significantly greater than that of the normal group (P < 0.001); compared with the model group, on the second day of administration, the fecal index of suckling mice in the ribavirin group (P < 0.001), high-dose group (P < 0.001), and medium-dose group (P < 0.01) decreased significantly. On the third day of administration, the fecal index of suckling mice in the ribavirin group (P < 0.001), high-dose group (P < 0.001), medium-dose group (P < 0.01), and low-dose group (P < 0.05) decreased significantly. On the fourth day of administration, the fecal index of suckling mice in the ribavirin group (P < 0.001), high-dose group (P < 0.001), medium-dose group (P < 0.01), and low-dose group (P < 0.01) decreased significantly. On the 5th day of administration, the fecal indexes of suckling mice in the ribavirin group (P < 0.001), high-dose group (P < 0.001), medium-dose group (P < 0.01), and low-dose group (P < 0.01) were significantly decreased. The results are shown in Table 2.
[0067] Table 1 Comparison of body weight of suckling mice at different time points after administration ( ±s, n=10)
[0068]
[0069] Note: Compared with the normal group, ***P<0.001; compared with the model group, #P<0.05, ##P<0.01, ###P<0.001.
[0070] Table 2 Diarrhea scores of suckling mice at different time points after administration ( ±s, n=10)
[0071]
[0072] Note: Compared with the normal group, *** P<0.001; compared with the model group, # P<0.05, ## P<0.01, ### P<0.001.
[0073] The traditional Chinese medicine composition provided by the invention has a certain effect of resisting diarrhea of suckling mice caused by rotavirus infection, improving the physical signs of infected suckling mice, promoting weight gain, reducing the feces index of suckling mice, and alleviating the degree of diarrhea.
[0074] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.
Claims
1. Use of a Chinese medicine composition in the preparation of a drug for treating and / or preventing viral infectious diarrhea; The Chinese medicine composition comprises: The invention discloses one or a combination of two or more of 1-100 parts of Magnolia officinalis bark, 1-100 parts of burnt Areca catechu, 1-100 parts of stewed Tsaoko fruit, 1-100 parts of Ephedra sinica, 1-100 parts of Bitter Apricot, 1-100 parts of Notopterygium wilfordii, 1-100 parts of Ginger, 1-100 parts of Patchouli, 1-100 parts of Perilla frutescens, 1-100 parts of Atractylodes macrocephala, 1-160 parts of Poria cocos, 1-120 parts of Atractylodes macrocephala, 1-100 parts of Gypsum, 1-100 parts of burnt Crataegus pinnatifida, 1-100 parts of burnt Liu Shenqu, 1-100 parts of burnt malt, 1-100 parts of Pheretima, 1-100 parts of Cynanchum cyrtonema, 1-100 parts of Cynomorium wilfordii and / or 1-100 parts of Semen Lepidium.
2. The use according to claim 1, characterized in that The treatment or prevention of viral infectious diarrhea includes any of the following: (I) Improve abdominal pain; and / or (II), improve diarrhea; and / or (III) Improve vomiting; and / or (IV) Improve animal physical signs.
3. The use according to claim 1 or 2, characterized in that: The improvement of animal signs also includes any of the following: (I) Improve body mass; and / or (II) Reduce fecal index.
4. The use according to claim 1 or 3, characterized in that: The improving body mass includes promoting weight gain.
5. The use according to claims 1 to 4, characterized in that The viruses include rotavirus.
6. The use according to any one of claims 1 to 5, characterized in that The Chinese medicine composition comprises: 10-80 parts of Magnolia officinalis, 10-80 parts of charred Areca catechu, 10-80 parts of stewed Tsaoko fruit, 10-60 parts of Ephedra sinica, 10-60 parts of Apricot kernel, 10-80 parts of Notopterygium wilfordii, 10-60 parts of ginger, 10-80 parts of Patchouli, 10-60 parts of Cyperus rotundus, 10-80 parts of Atractylodes macrocephala, 10-160 parts of Poria cocos, 10-120 parts of Atractylodes macrocephala, 10-80 parts of Gypsum, 10-50 parts of charred Crataegus pinnatifida, 10-80 parts of charred Liu Shenqu, 10-60 parts of charred malt, 10-80 parts of Pheretima, 10-80 parts of Cynomorium wilfordii, 10-60 parts of Cynomorium fortunei and / or 10-80 parts of Lepidium chinense.
7. The use according to any one of claims 1 to 6, characterized in that The Chinese medicine composition comprises: 30-50 parts of Magnolia officinalis, 20-30 parts of burnt betel nut, 30-50 parts of stewed tsaoko, 20-30 parts of ephedra, 20-30 parts of bitter almond, 30-50 parts of notopterygium, 30-50 parts of ginger, 30-50 parts of patchouli, 20-30 parts of perilla, 30-50 parts of atractylodes, 120-150 parts of tuckahoe, 80-100 parts of atractylodes, 30-50 parts of gypsum, 20-30 parts of burnt hawthorn, 30-50 parts of burnt six-spirit song, 20-30 parts of burnt malt, 30-50 parts of earthworm, 30-50 parts of cynanchum chinense, 20-30 parts of mianma cyperus, and 30-50 parts of scutellaria baicalensis. Preferentially, one or a combination of two or more of 50 parts of Magnolia officinalis, 30 parts of scorched Areca nut, 30 parts of stewed Tsaoko fruit, 20 parts of Ephedra, 30 parts of Bitter Apricot, 50 parts of Notopterygium wilfordii, 50 parts of Ginger, 50 parts of Patchouli, 30 parts of Perilla frutescens, 50 parts of Atractylodes macrocephala, 150 parts of Poria, 100 parts of Atractylodes macrocephala, 50 parts of Gypsum, 30 parts of scorched Crataegus pinnatifida, 30 parts of scorched Liu Shenqu, 30 parts of scorched malt, 50 parts of Pheretima, 50 parts of Cynanchum chinense, 30 parts of Cynomorium mongolicum and / or 50 parts of Semen Lepidium.
8. The use according to any one of claims 1 to 7, characterized in that The preparation method of the traditional Chinese medicine composition comprises: taking one or a combination of two or more of magnolia bark, burnt betel nut, stewed tsaoko, ephedra, bitter almond, notopterygium wilfordii, ginger, patchouli, perilla, atractylodes, tuckahoe, atractylodes macrocephala, gypsum, burnt hawthorn, burnt six-shenqu, burnt malt, earthworm, cynanchum chinense, mianma guanzhong and / or scutellaria baicalensis seed in a prescribed amount, refluxing extraction, filtering, concentrating, centrifuging, drying and pulverizing to obtain an intermediate composition; Taking the intermediate composition and mixing it with pharmaceutically acceptable excipients to prepare the Chinese medicine composition; The reflux extraction includes: adding 6 times of water for the first time and extracting for 1.5 hours, and adding 4 times of water for the second time and extracting for 1.0 hour.
9. The use according to any one of claims 1 to 8, characterized in that The effective dose of the drug includes 9.36-37.44 g crude drug / kg mouse body weight.
10. The use according to any one of claims 1 to 9, characterized in that The administration method of the drug includes: one or more of oral administration, injection or external application.