Loose two-season bamboo rooting agent as well as preparation method and application thereof

Abstract

The invention discloses a loose two-season bamboo rooting agent as well as a preparation method and application thereof. The rooting agent is prepared from the following raw materials with final concentration: 200 to 400mg / L of GGR6, 200 to 400mg / L of potassium nitrate and 200 to 400mg / L of boric acid. The rooting agent for the loose two-season bamboos can effectively promote the rooting rate of the loose two-season bamboos, and the rooting rate reaches 80%.

Description

Technical Field

[0001] The invention belongs to the technical field of rooting agents, and in particular relates to a scattered-type bi-seasonal bamboo rooting agent and a preparation method and application thereof. Background Art

[0002] The rhizome systems of different bamboo species vary greatly and can be divided into three main categories: sympodial type, in which the buds on the base of the culm directly sprout into bamboo shoots, and the above-ground culms densely form clumps, which are generally called clumping bamboos; monopodial type, in which the buds on the base of the culm first form nodes, on which there are buds and roots that can continuously extend underground, and some of the buds on the nodes directly become bamboo shoots, while others form new bamboo whips. Therefore, the distances between the above-ground bamboo culms are relatively far apart, and this is generally called scattered bamboo; polypodial type: with the characteristics of sympodial and monopodial rhizomes, it is also generally called mixed bamboo.

[0003] Generally speaking, bamboo can produce shoots all year round as long as the conditions are right, but there is usually only one peak period for producing shoots a year, while scattered biseasonal bamboo has two peak periods for producing shoots a year.

[0004] At present, scattered bi-seasonal bamboos are mostly propagated by dividing the stumps and burying the rhizomes. Burying the rhizomes and dividing the stumps is time-consuming and labor-intensive, and causes great damage to the mother bamboo. Burying the stalks avoids damage to the underground stems and is convenient and quick, but the rooting rate is low. Therefore, how to reduce the damage to the mother bamboo and increase the rooting rate has become a problem that needs to be solved urgently in the field of scattered bi-seasonal bamboo breeding. Summary of the invention

[0005] The invention aims to provide a scattered two-season bamboo rooting agent and a preparation method and application thereof. The rooting agent can reduce the damage of buried-stem propagation to mother bamboo and improve the rooting rate.

[0006] In a first aspect, the present invention provides a scattered two-season bamboo rooting agent, which comprises the following raw materials with final concentrations: GGR6 is 200-400 mg / L, potassium nitrate is 200-400 mg / L, and boric acid is 200-400 mg / L.

[0007] Furthermore, ABT1 is included, and the final concentration of ABT1 is 200-400 mg / L.

[0008] Preferably, the rooting agent is composed of the following raw materials with final concentrations: 200 mg / L ABT1, 200 mg / L GGR6, 200 mg / L potassium nitrate, and 200 mg / L boric acid.

[0009] In a second aspect, the present invention provides the use of the rooting agent described in the first aspect in improving the rooting rate of buried stalks of scattered two-season bamboo.

[0010] Furthermore, the method for using the scattered two-season bamboo rooting agent comprises the following steps:

[0011] ①Cut the selected mother bamboo, cut off 3-5 nodes, keep 2-3 branches on the main branches, and cut off the rest;

[0012] ② Dig trenches with a spacing of 25cm-35cm and a width of 10cm-15cm on the prepared seedbed, or dig a 1m-1.2m seedbed;

[0013] ③ Soak the bamboo nodes prepared in step ① in the prepared rooting agent, then lay the nodes flat in the trench dug in step ②, with the branches and buds on the nodes facing both sides, and cover with 5cm-8cm of soil.

[0014] Furthermore, the mother bamboo is a 1-2 year old, robust, disease-free, and insect-free scattered two-season bamboo. The 1-2 year old bamboo has high nutrient and water content and can provide sufficient nutrients for the germination of new plants. Retaining 2-3 nodes can not only provide a large number of new plants, but also ensure the supply of nutrients.

[0015] Furthermore, the prepared bamboo nodes are soaked in the prepared rooting agent for 10-30 minutes, preferably 10 minutes.

[0016] Furthermore, after covering the soil, water it every 3-5 days depending on weather conditions to keep the soil moist.

[0017] The advantages and beneficial effects of the present invention are:

[0018] The invention provides a rooting agent for scattered two-season bamboo. The rooting agent is based on the growth characteristics of scattered two-season bamboo. ABT1, GGR6, potassium nitrate and boric acid are used as raw materials. Potassium nitrate, boric acid, ABT1 and GGR6 cooperate together to play a synergistic effect, which can improve the rooting rate, increase the root surface area, root length and the number of roots, improve the activity of root enzymes, enhance the metabolic capacity, induce root germination, promote the absorption of nutrients and protein synthesis, and enhance the activity of intracellular enzymes. The generation and transport of organic acids can be regulated, the differentiation and growth of root cells can be promoted, and the cell sugar metabolism can be promoted. The rooting agent of the invention can effectively promote the rooting rate and root length of scattered two-season bamboo, so that the rooting rate reaches 80% and the root length reaches 11.47cm. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is the appearance of the root system of some scattered two-season bamboos after the rooting agent described in Example 5 was used to bury the stalks for 90 days;

[0020] Figure 2 This is a detailed diagram of the root length of the scattered two-season bamboo with the shortest root length 90 days after being buried in the stalk with the rooting agent described in Example 5. DETAILED DESCRIPTION

[0021] The technical solutions in the embodiments of the present application are clearly described below. Obviously, the described embodiments are part of the embodiments of the present application, rather than all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments in the present application belong to the scope of protection of the present application.

[0022] The experimental methods in the following examples, unless otherwise specified, are all conventional methods, and are performed according to the techniques or conditions described in the literature in the field or according to the product instructions. The materials, reagents, etc. used in the following examples, unless otherwise specified, can all be obtained from commercial channels.

[0023] ABT1 was purchased from Beijing Abidi Biotechnology Co., Ltd.;

[0024] GGR6 was purchased from Beijing Abidi Biotechnology Co., Ltd.;

[0025] Potassium nitrate and boric acid were from Aladdin and were analytically pure.

[0026] Experimental Example 1:

[0027] 1. Experimental materials:

[0028] The experimental object is the scattered two-season bamboo. This scattered two-season bamboo is a new variety of bamboo plant that grows in Daguan County, Yunnan Province, and is obtained through artificial selection. Its shoots are tender and fleshy, and there are two peak periods of shoot growth in a year. It is a new variety of scattered bamboo with high-quality shoots.

[0029] This variety is made public through reference (Jia Chengxinzhuo, Long Yuanli, Sun Maosheng. High-quality bamboo shoots 'Spring and Autumn No. 1' [J]. Acta Horticulturae Sinica, 2023, 50(S2): 99-100. DOI: 10.16420 / j.issn.0513-353x.2023-0619.).

[0030] 2. Experimental methods:

[0031] Experimental reagents:

[0032] ABT1 rooting powder (three concentrations): Dissolve ABT1 in water to prepare solutions with concentrations of 100 mg / L, 200 mg / L, and 400 mg / L respectively;

[0033] GGR6 rooting powder (three concentrations): Dissolve GGR6 in water to prepare solutions with concentrations of 100 mg / L, 200 mg / L, and 400 mg / L respectively;

[0034] Potassium nitrate (three concentrations): Dissolve potassium nitrate in water to prepare solutions with concentrations of 100 mg / L, 200 mg / L, and 400 mg / L respectively;

[0035] Boric acid (three concentrations): Dissolve boric acid in water and prepare solutions with concentrations of 100 mg / L, 200 mg / L, and 400 mg / L respectively.

[0036] This experiment investigates the effects of four rooting agents (ABT1 rooting powder (three concentrations), GGR6 rooting powder (three concentrations), potassium nitrate (three concentrations), and boric acid (three concentrations) on the rooting of scattered two-season bamboo. Each concentration corresponds to 20 bamboos. The stalks were buried according to the method described in Example 1, and the root system indicators of scattered two-season bamboo were measured after 90 days, including rooting rate and root length. The measurement results (average values) are shown in Table 1.

[0037] Table 1

[0038]

[0039]

[0040] It can be seen from Table 1 that when ABT1, GGR6, potassium nitrate and boric acid are used alone as rooting agents, although the longest root length can reach 6.93, the rooting rate can only reach 20%. Although the highest rooting rate can reach 80%, the root length can only reach 2.84.

[0041] Example 1

[0042] This embodiment provides a scattered two-season bamboo rooting agent, which is composed of the following raw materials with final concentrations: GGR6 is 400 mg / L, potassium nitrate is 400 mg / L, and boric acid is 400 mg / L.

[0043] A preparation method of a scattered two-season bamboo rooting agent:

[0044] Weigh 2000 mg of GGR6, potassium nitrate and boric acid respectively, dissolve them in 5 L of water and mix well.

[0045] The method for using the scattered two-season bamboo rooting agent of this embodiment comprises the following steps:

[0046] 1. During March to June or September to October every year, select the bamboo stalks of the two-year-old scattered bamboo of two-season bamboo that is healthy and strong, free of pests and diseases, and after the bamboo shoots are sent out as mother bamboos, cut and select the mother bamboo, intercept 3-5 sections, and keep 2-3 branches on the main branches of the sections, and the rest are cut off. In the present embodiment, select the mother bamboo of the two-season bamboo that is one year old, healthy and strong, free of pests and diseases. Cut and select the mother bamboo, intercept 4 sections, and keep 3 branches on the main branches of the sections, and the rest are cut off.

[0047] ② On the arranged seedling bed, dig a ditch with a spacing of 25cm-35cm and a width of 10cm-15cm according to the diameter of the bamboo stalks, or dig a 1m-1.2m seedling bed. This embodiment adopts a ditch with a spacing of 30cm and a width of 12cm on the arranged seedling bed according to the diameter of the bamboo stalks.

[0048] ③ Soak the bamboo nodes prepared in step ① in the prepared rooting agent for 10 minutes, then lay the nodes flat in the trench dug in step ②, with the buds on the nodes facing both sides, and cover with 5cm-8cm of soil. Keep the soil moist after covering, and water it every 3-5 days depending on the weather conditions.

[0049] Example 2

[0050] This embodiment provides a scattered two-season bamboo rooting agent, which is composed of the following raw materials with final concentrations: GGR6 is 300 mg / L, potassium nitrate is 300 mg / L, and boric acid is 300 mg / L. The rest is the same as in Example 1.

[0051] Example 3

[0052] This embodiment provides a scattered two-season bamboo rooting agent, which is composed of the following raw materials with final concentrations: GGR6 is 350 mg / L, potassium nitrate is 350 mg / L, and boric acid is 350 mg / L. The rest is the same as in Example 1.

[0053] Example 4

[0054] This embodiment provides a scattered two-season bamboo rooting agent, which is composed of the following raw materials with final concentrations: ABT1 is 400 mg / L, GGR6 is 400 mg / L, potassium nitrate is 400 mg / L, and boric acid is 400 mg / L. The rest is the same as in Example 1.

[0055] Example 5

[0056] This embodiment provides a scattered two-season bamboo rooting agent, which is composed of the following raw materials with final concentrations: ABT1 is 200 mg / L, GGR6 is 200 mg / L, potassium nitrate is 200 mg / L, and boric acid is 200 mg / L. The rest is the same as in Example 1.

[0057] like Figure 1 As shown, Figure 1 The ruler used in the experiment is 20 cm long. After 90 days of burying the stalks with the rooting agent described in this embodiment, the longest root length of the scattered two-season bamboo has exceeded 20 cm. Figure 2 As shown, after the rooting agent described in this embodiment is used to bury the bamboo stems for 90 days, the shortest root length of the scattered two-season bamboo is measured, and its root length can also reach 9 cm.

[0058] Comparative Example 1

[0059] This comparative example provides a scattered two-season bamboo rooting agent, which is composed of the following raw materials with final concentrations: ABT1 is 100 mg / L, GGR6 is 100 mg / L, potassium nitrate is 100 mg / L, and boric acid is 100 mg / L. The rest is the same as Example 5.

[0060] Comparative Example 2

[0061] This comparative example provides a scattered two-season bamboo rooting agent, which is composed of the following raw materials with final concentrations: GGR6 is 200 mg / L, and boric acid is 200 mg / L. The rest is the same as Example 5.

[0062] Comparative Example 3

[0063] This comparative example provides a scattered two-season bamboo rooting agent, which is composed of the following raw materials with final concentrations: GGR6 is 200 mg / L, potassium nitrate is 200 mg / L. The rest is the same as Example 5.

[0064] Comparative Example 4

[0065] This comparative example provides a scattered two-season bamboo rooting agent, which is composed of the following raw materials with final concentrations: ABT1 is 600 mg / L, GGR6 is 600 mg / L, potassium nitrate is 600 mg / L, and boric acid is 600 mg / L, and the rest is the same as Example 5.

[0066] Experimental Example 2:

[0067] 1. Experimental materials:

[0068] The scattered biseasonal bamboo was used as the experimental object.

[0069] 2. Experimental methods:

[0070] Experimental reagents:

[0071] The rooting agent prepared in Example 1-5;

[0072] Rooting agents prepared in Comparative Examples 1-4.

[0073] This experiment set up 9 groups to measure the effect of the rooting agent prepared in Examples 1-5 and Comparative Examples 1-4 on the rooting of scattered two-season bamboo buried stems, and each group corresponded to 20 bamboos. The stems were buried according to the method described in Example 1, and the root system indicators of scattered two-season bamboo were measured after 90 days, including rooting rate and root length. The measurement results (average values) are shown in Table 2.

[0074] Table 2

[0075] Group Root length (cm) Rooting rate (%) Example 1 8.65 60 Example 2 8.08 60 Example 3 8.12 60 Example 4 8.74 60 Example 5 11.47 80 Comparative Example 1 5.36 60 Comparative Example 2 4.44 40 Comparative Example 3 4.64 40 Comparative Example 4 3.28 30

[0076] As shown in Table 2, compared with the comparative examples 1-4, the rooting agents prepared in Examples 1-5 can significantly increase the root length of the two-season bamboo rooting, especially the rooting agent in Example 5 has the best rooting effect, with a rooting rate of 80% and a root length of 11.47 cm. As shown in Table 1, when ABT1 with a concentration of 400 mg / L is used alone as a rooting agent, its rooting effect is 0, but from the comparison between Example 4 and Example 1, it can be seen that when ABT1 with a concentration of 400 mg / L is added to the rooting agent prepared in Example 1, the root length increases from 8.65 to 8.74, which may be due to the synergistic effect between ABT1 with a concentration of 400 mg / L, GGR6 with a concentration of 400 mg / L, potassium nitrate with a concentration of 400 mg / L and boric acid with a concentration of 400 mg / L, thereby increasing the root length.

[0077] By comparing Examples 1-3, it can be seen that when the rooting agent consists only of GGR6, potassium nitrate and boric acid, the rooting effect increases with the increase in the concentrations of the three components, and the root length increases from 8.08 to 8.65.

[0078] By comparing Examples 4-5 and Comparative Example 1, it can be seen that when the ABT1 component is added to the rooting agent, the rooting effect is improved as the concentrations of the four components decrease. When the concentrations of ABT1, GGR6, potassium nitrate, and boric acid are all 200 mg / L, the rooting effect is the best, the root length can reach 11.47, and the rooting rate reaches 80%. When the concentrations of ABT1, GGR6, potassium nitrate, and boric acid are further reduced to 100 mg / L, the rooting effect is greatly reduced, the rooting rate is only 60%, and the root length can only reach 5.36.

[0079] The rooting rate and root length of Comparative Example 2 were significantly lower than those of Example 5 due to the lack of potassium nitrate and ABT1 components, and Comparative Example 3 was significantly lower than those of Example 5 due to the lack of boric acid and ABT1 components. The rooting effect of Comparative Example 4 was poor due to the excessively high concentrations of ABT1, GGR6, potassium nitrate and boric acid.

[0080] The embodiments of the present application are described above, but the present application is not limited to the above-mentioned specific implementation modes. The above-mentioned specific implementation modes are merely illustrative and not restrictive. Under the guidance of the present application, ordinary technicians in this field can also make many forms without departing from the purpose of the present application and the scope of protection of the claims, all of which are within the protection of the present application.

Claims

1. A scattered-type two-season bamboo rooting agent, characterized in that: The rooting agent includes the following raw materials with final concentrations: GGR6 is 200-400 mg / L, potassium nitrate is 200-400 mg / L, and boric acid is 200-400 mg / L.

2. A scattered-type two-season bamboo rooting agent according to claim 1, characterized in that: ABT1 is also included, and the final concentration of ABT1 is 200-400 mg / L.

3. A scattered-type two-season bamboo rooting agent according to claim 2, characterized in that: The rooting agent is composed of the following raw materials with final concentrations: 200 mg / L of ABT1, 200 mg / L of GGR6, 200 mg / L of potassium nitrate, and 200 mg / L of boric acid.

4. Use of the rooting agent according to any one of claims 1 to 3 in improving the rooting rate of buried stalks of scattered two-season bamboo.

5. The use according to claim 4, characterized in that The method for using the scattered two-season bamboo rooting agent comprises the following steps: ①Cut the selected mother bamboo, cut off 3-5 nodes, keep 2-3 branches on the main branches, and cut off the rest; ② Dig trenches with a spacing of 25cm-35cm and a width of 10cm-15cm on the prepared seedbed, or dig a 1m-1.2m seedbed; ③ Soak the bamboo nodes prepared in step ① in the prepared rooting agent, then lay the nodes flat in the trench dug in step ②, with the branches and buds on the nodes facing both sides, and cover with 5cm-8cm of soil.

6. The use according to claim 5, characterized in that: The mother bamboo is a 1-2 year old, robust, disease- and insect-free, scattered two-season mother bamboo.

7. The use according to claim 5, characterized in that: The prepared bamboo nodes are placed in the prepared rooting agent and soaked for 10-30 minutes, preferably 10 minutes.

8. The use according to claim 5, characterized in that: After covering the soil, water it every 3-5 days to keep the soil moist.

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