Composition for supporting nutrition of middle-aged and elderly pets and preparation method of composition

Abstract

The invention discloses a composition for supporting nutrition of middle-aged and elderly pets. The composition comprises the following components in percentage by mass: 8-16% of haematococcus pluvialis powder; 0.5-3% of lactoferrin; 8-16% of a ginkgo leaf extract; 8 to 16% of L-carnitine; 12 to 20% of beta-yeast glucan; 3-9% of tea polyphenol; 3-9% of nannochloropsis oculata oil; 12 to 20% of schizochytrium limacinum powder; 3-9% of taurine; 0.02 to 0.1 percent of vitamin D3; 0.5-2% of vitamin E; and 3-9% of vitamin C phosphate. Therefore, the disease resistance of the middle-aged and old pets is improved, the cognitive decline condition of the middle-aged and old pets is improved, and according to the molecular characteristics of brain structures, namely rich expression of lactoferrin receptors on brain microvascular endothelial cells and blood brain barriers, lactoferrin is additionally added to serve as a ligand substance to modify substances with brain functions, so that the brain function of the middle-aged and old pets is improved, and the cognitive decline condition of the middle-aged and old pets is improved. Therefore, the lactoferrin is utilized by brain cells through brain microvascular endothelial cells and lactoferrin receptors on a blood brain barrier.

Description

Technical Field

[0001] The invention belongs to the technical field of pet breeding, and in particular relates to a composition for nutritional support of middle-aged and elderly pets and a preparation method thereof. Background Art

[0002] The passing of the peak of pet keeping has brought about the outbreak of pet aging. The most prominent problem of pet aging is low immunity leading to an increase in the incidence of pet diseases, and the deterioration of cognitive ability leading to abnormal pet behavior and cognition, thereby weakening the companionship attributes of pets. These aging problems will hinder pet owners throughout their lives, because people who keep pets now are more willing to regard pets as family members, rather than just pets. However, the life of a pet is very short. When a pet gradually ages, the internal body of the pet has changed and is facing aging. As the pet ages, various body functions decline rapidly, and disease threats ensue. Patients are problems and challenges that pet owners need to face.

[0003] At present, when pets have aging problems, pet owners often feel helpless because they are unable to consciously supplement the nutrients needed for their pets from the time they enter middle-aged and elderly. This dilemma arises because there is currently no product in the pet market that is specifically designed for nutritional support for middle-aged and elderly pets.

[0004] Currently, there are no products on the market that can improve cognitive impairment in aging pets, and there are no elderly products that target the brain. Summary of the invention

[0005] In order to solve one or more of the above problems, the present invention provides a composition for nutritional support of middle-aged and elderly pets and a preparation method thereof.

[0006] According to one aspect of the present application, a composition for nutritional support of middle-aged and elderly pets is provided, wherein the components thereof include, by mass percentage: 8-16% of Haematococcus pluvialis powder; 0.5-3% of lactoferrin; 8-16% of ginkgo leaf extract; 8-16% of L-carnitine; 12-20% of β-yeast glucan; 3-9% of tea polyphenols; 3-9% of Nannochloropsis oil; 12-20% of Schizochytrium powder; 3-9% of taurine; 0.02-0.1% of vitamin D3; 0.5-2% of vitamin E; and 3-9% of vitamin C phosphate.

[0007] Its beneficial effects are:

[0008] The nutritional needs and safety of each component in the above-mentioned nutritional support composition for middle-aged and elderly pets are important factors in determining their dosage. For example, although L-carnitine can enhance neuronal metabolic capacity, protect nerves, resist oxidation and promote synaptic neurotransmission, it also has a slight digestive tract side effect - diarrhea. However, the applicant improves intestinal function and regulates intestinal flora by combining L-carnitine with β-yeast glucan, thereby synergistically solving the side effect of mild diarrhea caused by L-carnitine. The dosage needs to be sufficient to exert its synergistic effect, but not excessive to avoid causing side effects. Secondly, Omega 3 exists in many raw materials. It is known in the industry that Omega 3 can improve the individual's brain cognitive function. However, the applicant learned through studying the molecular characteristics of brain structure that pure Omega 3, such as fish oil and linseed oil, cannot be absorbed and utilized by brain cells. The applicant solves the problem that single-source omega 3 cannot be absorbed and utilized by brain cells by compounding Schizochytrium powder containing DHA and Nannochloropsis oil containing EPA, and solves the problem that DHA cannot pass through the blood-brain barrier by using Nannochloropsis oil containing polar lipids (which can be transported through the blood-brain barrier), and the amount of addition is added according to the demand for omega 3 by middle-aged and elderly dogs and cats. Finally, the applicant found that the selection of Haematococcus pluvialis powder, ginkgo leaf extract, L-carnitine, tea polyphenols, omega3, taurine, and lactoferrin in the composition can significantly improve the cognitive function of middle-aged and elderly pets. This may be due to the molecular characteristics of the brain structure - there are abundant expressions of lactoferrin receptors on brain microvascular endothelial cells and blood-brain barrier. The additional addition of lactoferrin as a ligand substance (Haematococcus pluvialis powder, ginkgo leaf extract, L-carnitine, tea polyphenols, omega3, taurine, etc.) to modify substances with brain functionality, the reason why lactoferrin can achieve the effect of brain targeted medication is that lactoferrin has emulsifying properties, which can emulsify and tightly connect the effective substances, so that they can be used by brain cells through lactoferrin receptors on brain microvascular endothelial cells and blood-brain barrier.

[0009] In some embodiments, the Haematococcus pluvialis powder is 13%, ginkgo leaf extract is 13%, L-carnitine is 13%, taurine is 6.5%, β-yeast glucan is 19%, lactoferrin is 1.5%, tea polyphenols is 6.5%, Nannochloropsis oil is 4.5%, Schizochytrium powder is 17.5%, vitamin D3 is 0.1%, vitamin E is 1.5%, and vitamin C phosphate is 3.5%.

[0010] According to another aspect of the present application, a method for preparing the aforementioned composition for nutritional support of middle-aged and elderly pets is provided, characterized in that it comprises the following steps:

[0011] S1. Weigh the raw materials by weight;

[0012] S2. Preparation of oil phase materials: Mix Haematococcus pluvialis powder, Schizochytrium powder, vitamin D3, Nannochloropsis oil, and vitamin E in a vacuum emulsifier and pour out the transfer barrel for later use;

[0013] S3. Preparation of aqueous phase materials:

[0014] S3.1 Place lactoferrin and purified water into a vacuum emulsifier, and continue stirring to ensure that lactoferrin is completely dissolved in the purified water, and heat to 60-85°C, and heat at 60-85°C for 15-30 minutes;

[0015] S3.2 Add β-yeast glucan and continue stirring for 0.5-1 hour. At this time, the lactoferrin-polysaccharide emulsification system has been formed.

[0016] S3.3 Add ginkgo leaf extract, L-carnitine, taurine, tea polyphenols, and vitamin C phosphate and continue stirring;

[0017] S4. Slowly adding the oil phase material to the aqueous phase material, and continuously stirring to fully mix the aqueous phase and the oil phase to obtain a mixture;

[0018] S5. The mixture is subjected to high-speed shearing and then high-pressure homogenization to obtain a lactoferrin emulsion loaded with the nutritional support composition for the middle-aged and elderly.

[0019] The beneficial effects are as follows: the oil-soluble material is first dissolved in the oil, so that the material is completely dissolved, which is more conducive to absorption by the body; lactoferrin is soluble in water, and when heated to 75°C, the emulsification performance is the best; β-yeast glucan is an effective ingredient in the nutritional support composition for the middle-aged and elderly, and is also a polysaccharide. When added to the lactoferrin solution, it can improve the emulsification performance of the lactoferrin solution and form a stable lactoferrin-polysaccharide double-layer emulsion. Other water-soluble materials are added later, so that the water-soluble materials can be dissolved in water, and the effective ingredients can be more easily absorbed by the body. The oil phase and the water phase (containing the lactoferrin-polysaccharide double-layer emulsion) can form an emulsified mixture with lactoferrin as the carrier through high-speed shearing and high-pressure homogenization.

[0020] In some embodiments, in the step S2, the stirring time is 0.5-1 hour, and the stirring rate is 1000-1800 r / min; and / or in the step S3.1, the stirring time is 1-2 hours, and the stirring rate is 1000-1800 r / min. and / or in the step S3.2, the stirring time is 0.5-1 hour, and the stirring rate is 1000-1800 r / min. and / or in the step S3.3, the stirring time is 0.5-1 hour, and the stirring rate is 1000-1800 r / min.

[0021] Its beneficial effect is: using the principle of like dissolves like, the water-soluble and oil-soluble materials are first dissolved, mixed, sheared, etc. respectively, to ensure that materials of the same nature are evenly mixed and the uniformity of the effective ingredients is guaranteed.

[0022] In some embodiments, in the step S4, the stirring time is 1 hour and the stirring rate is 1500 / min.

[0023] The beneficial effect is that the oil phase is slowly dripped into the water phase and continuously stirred to ensure that the oil phase material and the water phase material are fully and evenly mixed together, thereby ensuring the uniformity of the efficacy of the effective substance.

[0024] In some embodiments, in the step S5, the shear rate is 5000 r / min; the shear time is 5 minutes, the homogenization pressure is 0.4 MPa, and the homogenization treatment is 0.5 hours.

[0025] Its beneficial effect is that the water phase and oil phase materials flow at high speed through a cavity with a special internal structure (high-pressure homogenization cavity) under a certain pressure, causing a series of changes in the physical, chemical, and structural properties of the materials, ultimately achieving a homogenization effect.

[0026] The efficacy achieved by the technical solution of the present application is to improve the disease resistance of middle-aged and elderly pets and improve the cognitive decline of middle-aged and elderly pets. The present application improves the disease resistance of middle-aged and elderly pets, improves the cognitive decline of middle-aged and elderly pets, and according to the molecular characteristics of brain structure-there are abundant expressions of lactoferrin receptors on brain microvascular endothelial cells and blood-brain barrier, lactoferrin is additionally added as a ligand substance (Haematococcus pluvialis powder, ginkgo leaf extract, L-carnitine, tea polyphenols, omega3, taurine, etc.) to modify substances with brain functionality. The effect of targeted medication of the brain by lactoferrin is also attributed to the emulsification property of lactoferrin, which can emulsify and tightly connect the functional substances, so that they can be used by brain cells through the lactoferrin receptors on brain microvascular endothelial cells and blood-brain barrier.

[0027] Haematococcus pluvialis powder: contains natural astaxanthin. ① Astaxanthin is fat-soluble, it is easier to pass through the blood-brain barrier, which is more conducive to the treatment of central nervous system diseases, and can better protect nerves and improve cognitive function. ② Astaxanthin's antioxidant effect is one of the strongest natural products found so far. Astaxanthin also has a regulatory effect on the body's immunity, can increase serum antibody levels, and can increase the expression level of immune-related genes.

[0028] Lactoferrin: Lactoferrin receptors are abundantly expressed on brain microvascular endothelial cells and the blood-brain barrier. Lactoferrin is used as a ligand to modify substances with brain functions, and brain targeting is achieved by identifying the molecular characteristics on the blood-brain barrier. Lactoferrin has emulsifying properties, which can effectively promote the formation of a water-oil mixed system in the emulsion, tightly connect the water-oil interface, and act as an emulsifier. Lactoferrin can also improve the body's immunity and disease resistance.

[0029] Ginkgo leaf extract: contains proanthocyanidins, flavonoid glycosides, terpene lactones (ginkgolides + bilobalide) and a large amount of organic acids. It has the dual antioxidant effects of inducing the expression of antioxidant enzymes and directly scavenging free radicals. It can inhibit cerebral thrombosis, reduce oxygen free radicals, improve local blood circulation in the brain, reduce brain cell damage, protect the contralateral substantia nigra neurons and mitochondria, and increase local dopamine concentrations.

[0030] L-Carnitine: The root cause of cognitive problems in elderly dogs and cats is the abnormal expression of synapse-related proteins (astrocytes present in the brain). In addition to its ability to enhance neuronal metabolism in mitochondria, L-Carnitine also plays an important role in brain bioenergetics and neuroprotection through multiple mechanisms, including their antioxidant properties, and regulation and promotion of synaptic neurotransmission.

[0031] β-yeast glucan: Intestinal flora imbalance can cause metabolic dysfunction, leading to the accumulation of phenylalanine and isoleucine in peripheral blood. These metabolites stimulate the differentiation and proliferation of Th1 cells and infiltrate the brain, while activating microglia in the brain, leading to neuroinflammation and ultimately cognitive dysfunction. β-yeast glucan can reduce neuroinflammation in the brain by reshaping the disordered intestinal flora, regulating amino acid metabolism, and reducing the accumulation of phenylalanine and isoleucine, thereby improving cognitive dysfunction.

[0032] Tea polyphenols: Catechins, the main component of tea polyphenols, can alleviate the aging of nerve cells and delay the decline of nerve function, including the decline of cognitive function. Tea polyphenols also have antioxidant and free radical resistance effects, which can protect immune cells from damage by free radicals and enhance immunity.

[0033] Nannochloropsis oil, Schizochytrium powder: Nannochloropsis oil contains EPA, and Schizochytrium powder contains DHA; EPA and DHA are omega-3 fatty acids, which are important membrane components. They improve and prevent neurodegenerative diseases by increasing membrane fluidity, reducing oxidative stress, and inhibiting neuroinflammatory responses and apoptosis pathways. Nannochloropsis oil is rich in polar lipids that can be transported across the blood-brain barrier, and can be used as a carrier for fatty acid-based functional substances to cross the blood-brain barrier.

[0034] Taurine: Taurine is abundant in the brain and plays an important role in the central nervous system. It can act as a neurotransmitter and participate in the physiological activities of cells; it can act as a neurotrophic factor and participate in the development of the central nervous system; it can also act as an osmotic regulator and regulate calcium transport; at the same time, taurine is also a neuroprotectant, maintaining the integrity of the cell membrane structure, and has a protective effect on the neurotoxic effects of glutamate, endoplasmic reticulum stress, and mitochondrial disorders. Taurine affects the cognitive function of dementia through mechanisms such as anti-oxidation and neurotransmitter imbalance.

[0035] Vitamin D3: It can improve cognitive impairment in the aging body by regulating the amyloid protein production pathway, increasing synaptic plasticity, and reducing neuroinflammation and apoptosis.

[0036] Vitamin C phosphate and vitamin E: Natural antioxidants that help reduce free radical damage and resist tissue oxidative stress. Vitamin phosphate is more stable and will not degrade due to processing.

[0037] The applicant chose the above materials mainly because the root causes of cognitive problems in elderly pets are: ① abnormal expression of synapse (astrocytes present in the brain) related proteins; ② neuritis leading to neuronal death and brain cell apoptosis; ③ excessive free radicals in brain tissue, leading to nerve cell aging.

[0038] To solve the above problems, improvements can be made in the following aspects (corresponding to the above ①②③): ① Enhance synaptic plasticity and maintain mitochondrial function; ② Anti-inflammatory and reduce the production of inflammatory factors; ③ Remove free radicals and anti-oxidation.

[0039] Based on the above reasons, the applicant selects the following corresponding materials for proportioning (one-to-one correspondence with the above ①②③);

[0040] ① Ginkgo leaf extract: reduces oxygen free radicals, improves local blood circulation in the brain, reduces brain cell damage, protects contralateral substantia nigra neurons and mitochondria, and reduces the expression of inflammatory factors in nerve cells

[0041] L-Carnitine: In addition to their ability to enhance neuronal metabolism in mitochondria, they also play important roles in brain bioenergetics and neuroprotection through multiple mechanisms, including their antioxidant properties and regulation and promotion of synaptic neurotransmission.

[0042] Taurine: It is also a neuroprotective agent, maintaining the integrity of the cell membrane structure, and has a protective effect on the neurotoxic effects of glutamate, endoplasmic reticulum stress, and mitochondrial disorders.

[0043] DHA: can restore autophagy damage caused by oxidative stress, promote protective autophagy, and alleviate mitochondrial dysfunction.

[0044] ②Astaxanthin: Improves cognitive impairment mainly by reducing the release of inflammatory factors and anti-oxidation

[0045] β-yeast glucan: Studies have found that intestinal flora imbalance in cognitive impairment mouse models can cause metabolic dysfunction, leading to the accumulation of phenylalanine and isoleucine in the peripheral blood. These metabolites stimulate the differentiation and proliferation of Th1 cells and infiltrate the brain, while activating microglia in the brain, leading to the occurrence of neuroinflammation and ultimately cognitive dysfunction.

[0046] ③Tea polyphenols: can significantly increase the content and activity of SOD in serum and brain, thereby achieving anti-aging and improving cognitive effects.

[0047] In addition, the use of Haematococcus pluvialis powder, ginkgo leaf extract, L-carnitine, tea polyphenols, omega3, and taurine, supplemented with lactoferrin in the composition, can significantly improve the cognitive function of elderly pets. This is due to the molecular characteristics of the brain structure - there is abundant expression of lactoferrin receptors on the brain microvascular endothelial cells and the blood-brain barrier. The additional addition of lactoferrin as a ligand substance (Haematococcus pluvialis powder, ginkgo leaf extract, L-carnitine, tea polyphenols, omega3, taurine, etc.) modifies substances with brain functionality. The reason why lactoferrin can achieve the effect of targeted brain medication is that lactoferrin has emulsifying properties, which can emulsify and tightly connect the effective substances, so that they can be used by brain cells through the lactoferrin receptors on the brain microvascular endothelial cells and the blood-brain barrier.

[0048] The applicant found that although L-carnitine can enhance the metabolic capacity of neurons, protect nerves, resist oxidation and promote synaptic neurotransmission, it also has mild gastrointestinal side effects - diarrhea. However, the applicant has improved intestinal function and regulated intestinal flora by combining L-carnitine with β-yeast glucan, thereby synergistically solving the side effect of mild diarrhea caused by L-carnitine. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] Figure 1 A schematic diagram of a maze. DETAILED DESCRIPTION

[0050] 1. Implementation Examples

[0051] Example 1

[0052] The raw materials were weighed separately. The total amount of powdered raw materials was 100 kg, including 12 kg of Haematococcus pluvialis powder, 16 kg of ginkgo leaf extract, 16 kg of L-carnitine, 5 kg of taurine, 20 kg of β-yeast glucan, 1.48 kg of lactoferrin, 3 kg of tea polyphenols, 3 kg of Nannochloropsis oil, 20 kg of Schizochytrium powder, 0.02 kg of vitamin D3, 0.5 kg of vitamin E, 3 kg of vitamin C phosphate, and 20 kg of water.

[0053] Example 2

[0054] The raw materials were weighed separately. The total amount of powdered raw materials was 100 kg, including 13 kg of Haematococcus pluvialis powder, 13 kg of ginkgo leaf extract, 13.4 kg of L-carnitine, 6.5 kg of taurine, 19 kg of β-yeast glucan, 1.5 kg of lactoferrin, 6.5 kg of tea polyphenols, 4.5 kg of Nannochloropsis oil, 17.5 kg of Schizochytrium powder, 0.1 kg of vitamin D3, 1.5 kg of vitamin E, 3.5 kg of vitamin C phosphate, and 20 kg of water.

[0055] Example 3 - Lactoferrin-free

[0056] The raw materials were weighed separately. The total amount of powdered raw materials was 100 kg, including 13 kg of Haematococcus pluvialis powder, 13 kg of ginkgo leaf extract, 13.4 kg of L-carnitine, 6.5 kg of taurine, 19 kg of β-yeast glucan, 6.5 kg of tea polyphenols, 4.5 kg of Nannochloropsis oil, 17.5 kg of Schizochytrium powder, 0.1 kg of vitamin D3, 1.5 kg of vitamin E, 3.5 kg of vitamin C phosphate, and 20 kg of water.

[0057] Example 4

[0058] The raw materials were weighed separately. The total amount of powdered raw materials was 100 kg, including 16 kg of Haematococcus pluvialis powder, 8 kg of ginkgo leaf extract, 8 kg of L-carnitine, 9 kg of taurine, 12 kg of β-yeast glucan, 3 kg of lactoferrin, 9 kg of tea polyphenols, 9 kg of Nannochloropsis oil, 14.9 kg of Schizochytrium powder, 0.1 kg of vitamin D3, 2 kg of vitamin E, 9 kg of vitamin C phosphate, and 20 kg of water.

[0059] 2. Experimental part

[0060] (I) Dog and cat feeding experiment - test to improve immunity and disease resistance

[0061] Elderly dogs and cats between 9 and 10 years old were selected and fed adaptively, and then randomly divided into 4 groups according to body weight, with 10 animals in each group (half male and half female). There was a control group A and 4 compositions for nutritional support of middle-aged and elderly pets (hereinafter referred to as compositions) B, C, D, and E groups. The mixture was directly orally fed to the BE group, with a daily dosage of 0.5g / 5kg body weight.

[0062] A: Feed a fixed amount of food

[0063] B: Feeding a fixed amount of diet + composition - Example 2

[0064] C: Feeding a fixed amount of diet + composition - Example 1

[0065] D: Feeding a fixed amount of diet + composition - Example 3 without lactoferrin

[0066] E: Feeding a fixed amount of diet + composition - Example 4

[0067] The diets fed were all produced by the same manufacturer and with the same batch number; each group of dogs and cats were fed separately and had free access to drinking water.

[0068] 1. Experimental results:

[0069] 1.1 General observations: Overall, the behavioral activities, mental state, coat color, food and water intake of dogs and cats in the BE combination group were better than those in the control group during the four-week period, and B>C≈D≈E.

[0070] Table 1 - Comparison of canine lymphocyte immunophenotypes (n=10, )

[0071]

[0072]

[0073] Table 2 - Comparison of five immune indicators of cats (n = 10, )

[0074]

[0075] As shown in Table 3 and Table 4, compared with the control group, the total amount of T+B+NK-T lymphocytes, B lymphocytes and natural killer cells, Tlymph H / S (CD3, CD4 / CD3, CD Ratio)-the ratio of lymphocyte subsets H and S, and Total NK (CD16+ / CD56+)-the total amount of natural killer cells in Groups B, C and D were significantly different (with equal variance, P<0.05), and the overall trend of the data of these three indicators was higher in Group B than in other groups. This indicates that the composition has the effect of improving the immune level of dogs and cats.

[0076] Experimental conclusion: From the above, it can be seen that the combination can enhance immunity and improve disease resistance.

[0077] (II) Mouse model cognitive experiment

[0078] 50 C57 mice, male, with a body weight of 18-22g, free access to water and diet. The animals were randomly divided into 5 groups, 10 in each group, namely control group, model group, positive drug group, Example 2 group and Example 3 group; the animals in the drug group and model group were subcutaneously injected with D-galactose (150mg / kg) every day, and the control group was replaced with normal saline. At the same time, Example 2 group was gavaged with Example 2 every day, with a dose of 0.62g / kg; Example 3 group was gavaged with Example 3 every day, with a dose of 0.62g / kg, the positive drug group was gavaged with VC (100mg / kg), and the control group and the model group were gavaged with equal volumes of normal saline, and the administration was continued for 8 weeks.

[0079] Animals in water maze experiments have a tendency to explore and change. When leaving an arm, they always run to a new arm that they have not run to for a long time. Mice do not want to be in the water, so they look for stairs to climb out of the water. Mice can remember and find the route of the stairs after training. Training began the day after the last medication, and medication continued during the training. The water depth of the maze was 9 cm, the water temperature was 20°C, the training time was 120 s, and mice that did not reach the end within 120 s were counted as 120 s. They were blocked at the fork in the road 2 and trained twice, with an interval of 24 hours. The time required for the mice to reach the end and the number of errors made were recorded respectively (entering any blind end was counted as 1 error), see Figure 1 Schematic diagram of the middle maze.

[0080] (III) Biochemical index detection:

[0081] After blood collection, animals were killed by cervical dislocation, brain tissue and other organs were separated, washed with saline, dried with filter paper and weighed. Brain tissue was placed in 9 times its volume of saline, homogenized in an ice water bath using a glass homogenizer, and the homogenate was centrifuged at 4°C and 3 000r / min for 10min. The supernatant was taken to detect superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) in serum and brain, and lipofuscin content in brain tissue.

[0082] Statistical methods: SPSS19.0 statistical software was used for analysis, and the data were expressed as x±s. When the data met the normal distribution and the variance was equal, one-way analysis of variance was used, and P<0.05 indicated that the difference was statistically significant.

[0083] Mouse maze test: The first test was conducted immediately after training, and the arrival time and number of errors of the mice represented the learning ability of the mice. The second test was conducted one day after training, and the arrival time and number of errors represented the memory ability of the mice. The results are shown in Table 3.

[0084] Table 3 - Reaching time and number of errors represent the memory ability of mice

[0085]

[0086] Note: a means P < 0.01 (vs normal control group), b means P < 0.01 (vs model group), C means P < 0.01 (vs positive control group)

[0087] From Table 3, we can see that the arrival time of the mice in the model group was longer than that of the control group, the number of errors in the model group was more than that of the control group, and the arrival rate in the model group was less than that of the control group. The above three indicators were significantly different, proving that the memory and learning abilities of the D-galactose aging model mice were severely impaired and seriously decreased compared with normal mice, indicating that the experimental modeling was successful.

[0088] In the first test on the learning ability of mice, the arrival time data showed that the arrival time of the positive control group and Example 2 was significantly shorter than that of the model group, the arrival time of Example 2 was significantly shorter than that of the positive control group, and there was no significant difference between Example 3 (without lactoferrin) and the model group; the error number data showed that the number of errors in the positive control group and Example 2 was significantly less than that of the model group, the number of errors in Example 2 was significantly less than that of the positive control group, and there was no significant difference between Example 3 (without lactoferrin) and the model group; the achievement rate data showed that the achievement rates of the positive control group and Example 2 were significantly higher than those of the model group, the achievement rate of Example 2 was significantly higher than that of the positive control group, and there was no significant difference between Example 3 (without lactoferrin) and the model group.

[0089] In the second test on the memory ability of mice, the arrival time data showed that the arrival time of the positive control group and Example 2 was significantly shorter than that of the model group, the arrival time of Example 2 was significantly shorter than that of the positive control group, and there was no significant difference between Example 3 (without lactoferrin) and the model group; the number of errors data showed that the number of errors in the positive control group and Example 2 was significantly less than that of the model group, the number of errors in Example 2 was significantly less than that of the positive control group, and there was no significant difference between Example 3 (without lactoferrin) and the model group; the achievement rate data showed that the achievement rate of the positive control group and Example 2 was significantly higher than that of the model group, and there was no significant difference between Example 3 (without lactoferrin) and the model group.

[0090] The above data show that the composition of nutritional support for middle-aged and elderly pets is helpful for the learning ability and memory ability of aging mice, and has a certain degree of improvement on their cognitive function. The key to achieving this effect is to select Haematococcus pluvialis powder, ginkgo leaf extract, L-carnitine, tea polyphenols, omega3, and taurine, and to use lactoferrin in the composition at the same time, which can significantly improve the cognitive function of elderly pets. This may be due to the molecular characteristics of the brain structure - there are abundant expressions of lactoferrin receptors on brain microvascular endothelial cells and blood-brain barrier. The additional addition of lactoferrin as a ligand substance (Haematococcus pluvialis powder, ginkgo leaf extract, L-carnitine, tea polyphenols, omega3, taurine, etc.) modifies substances with brain functionality. The fact that lactoferrin can achieve the effect of targeted brain medication is also due to the fact that lactoferrin has emulsifying properties, which can emulsify and tightly connect the effective substances, so that they can be used by brain cells through lactoferrin receptors on brain microvascular endothelial cells and blood-brain barrier.

[0091] (IV) Effects of different groups on SOD, GSH-Px activity and MDA content in mouse serum and brain

[0092] Table 4 Effect of D-galactose on the SOD content in serum and brain of aging mice (x±s, n=10)

[0093]

[0094]

[0095] Note: a means P < 0.01 (vs normal control group), b means P < 0.01 (vs model group)

[0096] As shown in Table 4, the serum and brain SOD activities of the model group mice were significantly lower than those of the control group (P≤0.01), the serum SOD activities of the positive control group, Example 2 and Example 3 lactoferrin-free groups were significantly higher than those of the control group (P≤0.01), and the brain SOD activities were significantly higher in the positive control group and Example 2 groups (P≤0.01), and the brain SOD activity results were consistent with the behavioral experiments. In summary, the composition for nutritional support of middle-aged and elderly pets can significantly increase the SOD activities in serum and brain, and has obvious anti-aging effects;

[0097] Table 5 - Effects of D-galactose on GSH-Px levels in serum and brain of aging mice (x±s, n=10)

[0098] Group <![CDATA[Serum GSH-Px / U.mL -1 > <![CDATA[Brain GSH-Px / U.mL -1 > Control group 287.36±12.45 88.41±3.77 Model Group <![CDATA[258.13±13.31 a ]]> <![CDATA[77.62±4.05 a ]]> Positive control group <![CDATA[300.85±3.41 b ]]> <![CDATA[111.50±6.68 b ]]> Example 2 <![CDATA[373.70±3.11 b ]]> <![CDATA[171.25±5.03 b ]]> Example 3 - Lactoferrin-free <![CDATA[366.02±31.82 b ]]> <![CDATA[90.88±2.88 b ]]>

[0099] Note: a means P<0.01 (vs normal control group), b means P<0.01 (vs model group),

[0100] Glutathione peroxidase (GSH-Px) is an important free radical scavenging enzyme that can specifically catalyze the reduction reaction of reduced glutathione (GSH) to hydrogen peroxide, inhibit the formation of hydroxyl radicals by nicotinamide adenine dinucleotide, protect lipids from peroxidation by oxygen free radicals, and have the function of protecting the integrity of cell membrane structure and function. GSH-Px activity can reflect the aging of mice. As shown in Table 5, the GSH-Px activity in the serum and brain of the model group mice was significantly lower than that in the control group (P < 0.01), indicating that the D-galactose-induced aging mouse model was successful. The serum GSH-Px activity of the positive control group, Example 2 and Example 3 lactoferrin-free groups was significantly higher than that of the control group (P ≤ 0.01), and the brain GSH-Px activity was significantly higher in the positive control group and Example 2 groups (P ≤ 0.01), and the brain GSH-Px activity results were consistent with the behavioral experiment.

[0101] In summary, the composition for nutritional support of middle-aged and elderly pets can significantly increase the activity of GSH-Px in serum and brain, and has a significant anti-aging effect.

[0102] Table 6 - Effects of D-galactose on MDA content in serum and brain of aging mice (x±s, n=10)

[0103]

[0104]

[0105] Note: a means P < 0.01 (vs normal control group), b means P < 0.01 (vs model group)

[0106] Malondialdehyde (MDA), as a lipid oxidation product, can cause cross-linking polymerization of macromolecules such as proteins and nucleic acids, and has cytotoxicity. As shown in Table 6, the MDA content in the serum and brain of the model group mice was significantly higher than that in the control group (P < 0.01), indicating that the D-galactose-induced aging mouse model was successful. The serum MDA activity of the positive control group, Example 2 and Example 3 lactoferrin-free groups was significantly lower than that of the control group (P ≤ 0.01), and the brain GSH-Px activity was significantly increased in the Example 2 group (P ≤ 0.01), and the brain GSH-Px activity results were consistent with the behavioral experiments. In summary, the composition of nutritional support for middle-aged and elderly pets can significantly reduce the MDA content in serum and brain, and can reduce the degree of oxidative damage in the body.

[0107] Table 7 - Effects of D-galactose on lipofuscin content in the brain of aging mice (x±s, n=10)

[0108] Group <![CDATA[Lipofuscin / nmol.mL -1 > Control group <![CDATA[204.05±2.84 a ]]> Model Group <![CDATA[214.54±2.38 b ]]> Positive control group <![CDATA[164.69±3.12 b ]]> Example 2 <![CDATA[157.63±3.58 b ]]> Example 3 - Lactoferrin-free <![CDATA[190.65±1.73 b ]]>

[0109] Note: a means P < 0.01 (vs normal control group), b means P < 0.01 (vs model group)

[0110] The deposition of lipofuscin is a sign of aging. Changes in lipofuscin in the brain can effectively reflect the aging of the body. As shown in Table 7, the lipofuscin level in the brain of the model group mice was significantly higher than that of the control group (P < 0.01). The lipofuscin level in the brain of the positive control group and Example 2 was significantly lower than that of the model group, and the lactoferrin-free group of Example 3 was lower than that of the model group, but the reduction level was not as high as that of Example 2. In summary: The composition for nutritional support of middle-aged and elderly pets can reduce the lipofuscin content in the tissues of aging mice, thereby delaying the decline of brain cognitive function.

[0111] Combining the data in Tables 4, 5, 6 and 7, it is known that the lactoferrin-free group in Example 3 can significantly increase the levels of SOD and GSH-Px in the serum of aging mice, and can significantly reduce the level of MDA. However, the effect of lactoferrin-free group in Example 3 on SOD, GSH-Px, MDA and lipofuscin in the brain is not as good as that in Example 2. Therefore, Haematococcus pluvialis powder, ginkgo leaf extract, L-carnitine, tea polyphenols, omega3, and taurine can be selected and applied to the composition with the aid of lactoferrin to achieve brain targeted medication, which may be Due to the molecular characteristics of the brain structure - there is abundant expression of lactoferrin receptors on brain microvascular endothelial cells and the blood-brain barrier, lactoferrin is additionally added as a ligand substance (Haematococcus pluvialis powder, ginkgo leaf extract, L-carnitine, tea polyphenols, omega3, taurine, etc.) to modify substances with brain functionality. The reason why lactoferrin can achieve the effect of targeted brain medication is that lactoferrin has emulsifying properties, which can emulsify and tightly connect the effective substances, so that they can be used by brain cells through the lactoferrin receptors on the brain microvascular endothelial cells and the blood-brain barrier.

[0112] The above are only some embodiments of the present invention. It should be pointed out that for ordinary technicians in this field, other modifications and improvements can be made without departing from the creative concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. A composition for nutritional support of middle-aged and elderly pets, characterized in that: Its components by mass percentage include:

2. A composition for nutritional support of middle-aged and elderly pets according to claim 1, characterized in that: The composition comprises 13% of Haematococcus pluvialis powder, 13% of ginkgo leaf extract, 13.4% of L-carnitine, 6.5% of taurine, 19% of beta-yeast glucan, 1.5% of lactoferrin, 6.5% of tea polyphenols, 4.5% of Nannochloropsis oil, 17.5% of Schizochytrium powder, 0.1% of vitamin D3, 1.5% of vitamin E and 3.5% of vitamin C phosphate.

3. A method for preparing the composition for nutritional support of middle-aged and elderly pets according to any one of claims 1 to 2, characterized in that: The following steps are involved: S1. Weigh the raw materials by weight; S2. Preparation of oil phase materials: Mix Haematococcus pluvialis powder, Schizochytrium powder, vitamin D3, Nannochloropsis oil, and vitamin E in a vacuum emulsifier and pour out the transfer barrel for later use; S3. Preparation of aqueous phase materials: S3.1 Place lactoferrin and purified water into a vacuum emulsifier, and continue stirring to ensure that lactoferrin is completely dissolved in the purified water, and heat to 60-85°C, and heat at 60-85°C for 15-30 minutes; S3.2 Add β-yeast glucan and continue stirring for 0.5-1 hour. At this time, the lactoferrin-polysaccharide emulsification system has been formed. S3.3 Add ginkgo leaf extract, L-carnitine, taurine, tea polyphenols, and vitamin C phosphate, and continue stirring for 0.5-1h; S4. The oil phase material is slowly added to the water phase material, and stirring is continued to fully mix the water phase and the oil phase to obtain a mixture; S5. The mixture is subjected to high-speed shearing and then high-pressure homogenization to obtain a lactoferrin emulsion loaded with the nutritional support composition for the middle-aged and elderly.

4. The method according to claim 3, characterized in that In the step S2, the stirring time is 0.5-1 hour, and the stirring rate is: 1000-1800r / min; And / or in the step S3.1, the stirring time is 1-2 hours; the stirring rate is 1000-1800 r / min. And / or in the step S3.2, the stirring time is 0.5-1 hour; the stirring rate is 1000-1800 r / min. And / or in the step S3.3, the stirring time is 0.5-1 hour; the stirring rate is 1000-1800 r / min.

5. The method according to claim 3, characterized in that: In the step S4, the stirring time is 1 hour and the stirring rate is 1500 / min.

6. The method according to claim 3, characterized in that In the step S5, the shearing speed is 5000 r / min and the shearing time is 5 minutes.

7. The method according to claim 3, characterized in that In the step S5, the homogenization pressure is 0.4 MPa, and the homogenization process is performed for 0.5 hours.

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