A tea moth pheromone attractant and its application

Abstract

The invention discloses a tea moth pheromone attractant and its application, belonging to the technical field of pest control. The tea moth pheromone attractant comprises components of: 2E,13Z-18:OAc with CAS number 86252-74-6 and 2E,13Z-18:OH with CAS number 123551-47-3. The invention obtains an attractant with good attracting effect on tea moth by compounding 2E,13Z-18:OAc with 2E,13Z-18:OH, and the attractant also has a certain attracting effect on other lepidopteran insects.

Description

Technical Field

[0001] The invention belongs to the technical field of pest control, and particularly relates to a tea moth pheromone attractant and application thereof. Background Art

[0002] Pest attractants are an important technology for the development of green agriculture, and play an important role in the green prevention and control of pests. They are a technology with development prospects. At present, no corresponding pheromone attractants have been developed for the tea moth, a tea tree pest. Summary of the invention

[0003] In view of the above technical problems, the present invention provides a pheromone attractant for tea moth and its application. The present invention obtains an attractant with good attracting effect on tea moth by compounding 2E,13Z-18:OAc with 2E,13Z-18:OH, and the attractant also has a certain attracting effect on other lepidopteran insects.

[0004] To achieve the above object, the present invention provides the following technical solutions:

[0005] One of the technical solutions of the present invention is to provide a tea moth pheromone attractant, the components of which include: 2E, 13Z-18:OAc with a CAS number of 86252-74-6 and 2E, 13Z-18:OH with a CAS number of 123551-47-3.

[0006] Preferably, the volume ratio of the 2E,13Z-18:OAc to the 2E,13Z-18:OH is 1:9 to 9:1.

[0007] More preferably, the volume ratio of the 2E,13Z-18:OAc to the 2E,13Z-18:OH is 1:1.

[0008] The second technical solution of the present invention is to provide an attractant core, wherein the attractant used is the above-mentioned tea moth pheromone attractant

[0009] The third technical solution of the present invention is to provide a method for preparing the above-mentioned lure core, the steps comprising:

[0010] Heat carrageenan and water to melt and then cool to 50-55°C. Then add 2E,13Z-18:OAc and 2E,13Z-18:OH and stir evenly to obtain the bait core.

[0011] Preferably, the usage ratio of the carrageenan, water, 2E,13Z-18:OAc and 2E,13Z-18:OH is carrageenan:water:(total amount of 2E,13Z-18:OAc and 2E,13Z-18:OH)=0.3g:10mL:100μL.

[0012] The fourth technical solution of the present invention is to provide a use of the above-mentioned tea moth pheromone attractant or the above-mentioned attractant core in attracting the tea moth.

[0013] The beneficial technical effects of the present invention are as follows:

[0014] The invention obtains an attractant with good attracting effect on tea moth by compounding 2E,13Z-18:OAc and 2E,13Z-18:OH, and the attractant also has a certain attracting effect on other lepidopteran insects. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is one of the sticky boards of the trap with the bait prepared in Example 1 installed 10 days later.

[0016] Figure 2 One of the sticky boards of the trap equipped with the bait prepared in Example 2 10 days later.

[0017] Figure 3 One of the sticky boards of the trap with the bait prepared in Example 3 installed 10 days later.

[0018] Figure 4 One of the sticky boards of the trap with the bait prepared in Example 3 installed 10 days later.

[0019] Figure 5 This is one of the sticky boards of the trap equipped with the bait prepared in Comparative Example 1 10 days later.

[0020] Figure 6 This is one of the sticky boards of the trap equipped with the bait prepared in Comparative Example 2 10 days later.

[0021] Figure 7 This is one of the sticky boards of the trap equipped with the bait prepared in Comparative Example 3 10 days later. DETAILED DESCRIPTION

[0022] Now, various exemplary embodiments of the present invention are described in detail, and this detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, characteristics and embodiments of the present invention. It should be understood that the terms described in the present invention are only for describing specific embodiments and are not used to limit the present invention.

[0023] In addition, for the numerical range in the present invention, it is understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0024] Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which the invention pertains. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein may also be used in the practice or testing of the present invention.

[0025] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0026] The 2E,13Z-18:OAc and 2E,13Z-18:OH used in the examples and comparative examples of the present invention were purchased from BRUKER.

[0027] Example 1

[0028] Preparation of bait core:

[0029] Add 0.3 g of carrageenan to 10 mL of water, heat to melt, then cool to 50-55 °C, add 90 μL of 2E,13Z-18:OAc and 10 μL of 2E,13Z-18:OH, stir evenly, and fill into a rubber stopper after solidification to prepare a bait core for field trapping experiments.

[0030] Example 2

[0031] Preparation of bait core:

[0032] Add 0.3 g of carrageenan to 10 mL of water, heat to melt, then cool to 50-55 °C, add 10 μL of 2E,13Z-18:OAc and 90 μL of 2E,13Z-18:OH, stir evenly, and fill into a rubber stopper after solidification to prepare an attractant for field trapping experiments.

[0033] Example 3

[0034] Preparation of bait core:

[0035] Add 0.3 g of carrageenan to 10 mL of water, heat to melt, then cool to 50-55 °C, add 50 μL of 2E,13Z-18:OAc and 50 μL of 2E,13Z-18:OH, stir evenly, and fill into a rubber stopper after solidification to prepare an attractant for field trapping experiments.

[0036] Comparative Example 1

[0037] Preparation of bait core:

[0038] Add 0.3 g of carrageenan to 10 mL of water, heat to melt, then cool to 50-55 °C, add 100 μL of 2E,13Z-18:OAc, stir evenly, and fill into a rubber stopper after solidification to prepare a bait core for field trapping experiments.

[0039] Comparative Example 2

[0040] Preparation of bait core:

[0041] Add 0.3 g of carrageenan to 10 mL of water, heat to melt, then cool to 50-55 °C, add 100 μL of 2E,13Z-18:OH, stir evenly, and fill into a rubber stopper after solidification to prepare a bait core for field trapping experiments.

[0042] Comparative Example 3 (Blank Group)

[0043] Preparation of bait core:

[0044] Add 0.3 g of carrageenan to 10 mL of water, heat to melt, then cool to solidify, and fill it into a rubber stopper after solidification to prepare a bait core for field trapping experiments.

[0045] The bait cores prepared in Examples 1 to 3 and Comparative Examples 1 to 3 were used in field trapping experiments.

[0046] Each treatment was equipped with 20 bait cores, which were installed on boat-type traps. One bait core was installed on each trap. The traps were placed in the tea garden with each trap spaced 10 meters apart. After 10 days, the number of tea moths on the sticky plates (25×35 cm) of the traps was counted. The statistical results of tea moths are shown in Table 1.

[0047] Table 1 The attractant effect of each bait on the tea moth

[0048] Average number of insects caught by each sticky board (heads) Example 1 3.5 Example 2 3.8 Example 3 7.8 Comparative Example 1 0 Comparative Example 2 0 Comparative Example 3 (Blank) 0

[0049] Figure 1 This is one of the sticky boards of the trap with the bait prepared in Example 1 installed 10 days later.

[0050] Figure 2 One of the sticky boards of the trap equipped with the bait prepared in Example 2 10 days later.

[0051] Figure 3 One of the sticky boards of the trap with the bait prepared in Example 3 installed 10 days later.

[0052] Figure 4 One of the sticky boards of the trap with the bait prepared in Example 3 installed 10 days later.

[0053] Figure 5 This is one of the sticky boards of the trap equipped with the bait prepared in Comparative Example 1 10 days later.

[0054] Figure 6 This is one of the sticky boards of the trap equipped with the bait prepared in Comparative Example 2 10 days later.

[0055] Figure 7 This is one of the sticky boards of the trap equipped with the bait prepared in Comparative Example 3 10 days later.

[0056] Figure 1~Figure 4 In the middle, the circled insect is the tea moth; Figure 5~Figure 7 It shows that no tea moth was trapped.

[0057] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A pheromone attractant for tea moth, characterized in that: The components include: 2E,13Z-18:OAc with CAS number 86252-74-6 and 2E,13Z-18:OH with CAS number 123551-47-3; The volume ratio of the 2E,13Z-18:OAc to the 2E,13Z-18:OH is 1:9~9:

1.

2. The tea moth pheromone attractant according to claim 1, characterized in that The volume ratio of the 2E,13Z-18:OAc to the 2E,13Z-18:OH is 1:

1.

3. A lure, characterized in that: The attractant used is the tea moth pheromone attractant described in any one of claims 1 to 2.

4. A method for preparing the lure according to claim 3, characterized in that the steps include: Heat carrageenan and water to melt and then cool to 50-55°C. Then add 2E,13Z-18:OAc and 2E,13Z-18:OH and stir evenly to obtain the bait core.

5. The method for preparing the lure according to claim 4, characterized in that: The usage ratio of the carrageenan, water, 2E,13Z-18:OAc and 2E,13Z-18:OH is carrageenan:water:(total amount of 2E,13Z-18:OAc and 2E,13Z-18:OH)=0.3g:10mL:100μL.

6. Use of the pheromone attractant of any one of claims 1 to 2 or the attractant core of claim 3 in attracting the tea moth.

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