Traditional Chinese medicine composition for resisting bacteria and exudation of acute wound surface, medicinal preparation and preparation method

Abstract

The invention provides an antibacterial and anti-exudation traditional Chinese medicine composition for acute wounds, a pharmaceutical preparation and a preparation method, and relates to the technical field of traditional Chinese medicine, and the antibacterial and anti-exudation traditional Chinese medicine composition is prepared by dissolving radix scutellariae, cortex phellodendri, catechu and borneol in specific parts by weight in ethanol. The traditional Chinese medicine compound disclosed by the invention is a nationwide first batch of nationwide old traditional Chinese medicine showshoe empirical prescription, is clinically applied to more than 60%, is supported by a firm traditional Chinese medicine classical theory, is reasonable in component proportion, excellent in medicine compatibility, low in cost and easy in quality control, and is proved by animal and clinical experiments to be definite in curative effect, free of toxic and side effects, safe to take and suitable for clinical application. The traditional Chinese medicine composition can be applied to preparation of antibacterial and anti-exudation traditional Chinese medicine compositions for acute wounds.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicines, and in particular to a traditional Chinese medicine composition and a medicine preparation and a preparation method for antibacterial and anti-exudative treatment of acute wounds. Background Art

[0002] After burns and scalds and other traumas, due to the damage of the body's skin and other barriers, the capillary permeability increases, which will lead to acute wounds dominated by exudation. This provides a favorable environment for the growth of pathogenic microorganisms, and excessive pro-inflammatory stimulation will induce an "inflammatory factor storm" to further damage endothelial cells. The larger the area of ​​the trauma and the deeper the degree, the more damaged the skin's protective barrier is, and the more serious the pathogen infection is. The more serious consequence is that excessive exudation will dissolve the burns and scalds, and the dissolution of the burns means the destruction of the body's double barrier, resulting in the exudation of the injured and the aggravation of toxins reabsorbed into the blood, which is extremely harmful to the body and often causes the death of the injured. Therefore, it is particularly necessary to find antibacterial and anti-exudation Chinese medicines that can be used for acute wounds such as burns and scalds. Chinese medicine compositions have certain advantages in antibacterial and anti-exudation of acute wounds.

[0003] Traditional Chinese medicine believes that burns and scalds are caused by the invasion of fire poison. After the injury, the skin and fur are gone, the skin and defense are not solid, the meridians are burned, the body fluids are exuded, the qi is lost with the body fluids, the qi is deficient, the blood is stagnant, the body is deficient and the evil is strong, and finally the symptoms of fainting and reversal are caused. This is what modern medicine calls the destruction of the skin barrier after severe burns, which causes blood circulation disorders in the peripheral circulation, abnormal tissue metabolism, increased microvascular permeability, and functional disorders of the neurohumoral and cardiovascular systems. The damaged wound surface loses the protection of the skin, and the exuded tissue fluid provides a breeding ground for pathogen infection. As the tissue fluid gradually absorbs toxins back into the blood, the probability and severity of infection are increased. In the later stage, as the disease progresses, the body's transition from hyperimmunity to immunosuppression leads to uncontrolled systemic infection, which is an important reason for wound infection to cause multiple organ failure. Therefore, early intervention of exudation and infection in patients with acute wounds is the key to improving the efficacy and prognosis, and it is also an excellent entry point for traditional Chinese medicine intervention, which has important research value.

[0004] In view of this, the present invention is proposed. Summary of the invention

[0005] One of the purposes of the present invention is to provide a Chinese medicine composition for antibacterial and anti-exudation of acute wounds, so as to at least solve one of the technical problems existing in the prior art.

[0006] A second object of the present invention is to provide a pharmaceutical preparation.

[0007] The third object of the present invention is to provide a method for preparing the above-mentioned pharmaceutical preparation.

[0008] In order to achieve the above-mentioned purpose of the present invention, the following technical solutions are particularly adopted: The invention provides a traditional Chinese medicine composition for antibacterial and anti-exudation of acute wounds, comprising: 200-400 parts of scutellaria baicalensis, 200-400 parts of phellodendron chinense, 200-400 parts of catechu, and 100-200 parts of borneol, which are dissolved in 80% ethanol and quantitatively made into 1000 ml.

[0009] Furthermore, based on 1000 parts by weight, the following ingredients are included: 150-550 parts of Scutellaria baicalensis, 150-550 parts of Phellodendron amurense, 150-550 parts of Catechu, and 100-250 parts of Borneol, which are dissolved in 75%-80% ethanol and quantitatively adjusted to 1000 ml.

[0010] Furthermore, based on 1000 parts by weight, the composition includes: 300-500 parts of Scutellaria baicalensis, 300-500 parts of Phellodendron chinense, 300-500 parts of Catechu, and 100-300 parts of Borneol, which are dissolved in 75%-80% ethanol and quantitatively adjusted to 1000 ml.

[0011] The present invention also provides the use of the above-mentioned traditional Chinese medicine composition in the preparation of a pharmaceutical preparation for antibacterial and anti-exudation of acute wounds.

[0012] The present invention also provides a pharmaceutical preparation, which comprises an active ingredient and a pharmaceutically acceptable excipient; The active ingredients include the above-mentioned traditional Chinese medicine composition.

[0013] Furthermore, the dosage form of the pharmaceutical preparation includes tincture or spray.

[0014] The present invention also provides a method for preparing the above-mentioned pharmaceutical preparation, comprising processing the components of scutellaria baicalensis, phellodendron amurense and catechu into coarse powder, soaking them with 250 ml of 80% ethanol in a 100,000-class clean area environment, mixing them evenly, sealing them, and leaving them for 4 hours, and then loading them into a percolator with a filter material on the bottom, and soaking them in ethanol for 48-72 hours, and preparing them according to the percolation method (percolation speed is 4 ml / min), collecting 900 ml of the percolation liquid, adding borneol to dissolve it, adding ethanol to make it a full volume of 1000 ml, and sterilizing them with circulating steam at 100°C for 30 minutes to obtain the product.

[0015] Furthermore, it can also be made by the traditional soaking method, but it takes more than seven days, or it can be heated and refluxed in a distillation kettle, which only takes about 4 hours. After the extraction is completed, filter and wash the filter residue with 95% ethanol or aqueous ethanol to obtain.

[0016] Furthermore, the drug preparation is a spray. If 95% ethanol is used as a solvent, the obtained filtrate can be directly used in clinical practice. However, due to the high concentration of ethanol, it is highly irritating to the damaged wound surface and the patient has a severe pain. Therefore, distilled water should be added to the wound fluid to control the ethanol concentration to about 40-60%. The resulting drug solution can be directly filled into a spray bottle to obtain a spray.

[0017] Compared with the prior art, the present invention has the following beneficial effects: (1) The pharmaceutical composition of the present invention has solid theoretical support from classical Chinese medicine, conforms to the principle of monarch, minister, and adjuvant components of traditional Chinese medicine, forms a complete system of principles, methods, prescriptions, and medicines for acute wound exudation, and fills the gap in the theory of external treatment of acute wounds with traditional Chinese medicine at home and abroad; (2) Clinical application: This theory guides the formation of the drug combination of the present invention, which has been used in clinical treatment for more than 60 years, has a safe and reliable efficacy, no toxic side effects, and is safe to take. It can be used in the preparation of a Chinese medicine composition for antibacterial and anti-exudative use on acute wounds; (3) The drug of the present invention has excellent compatibility, reasonable ratio, low cost, and can reduce the economic and mental burden on patients, thus having a good prospect for promotion and application. DETAILED DESCRIPTION

[0018] Unless otherwise defined herein, scientific and technical terms used in conjunction with the present invention shall have the meanings commonly understood by those of ordinary skill in the art. The meaning and scope of the terms should be clear, however, in the case of any potential ambiguity, the definitions provided herein take precedence over any dictionary or external definitions. In this application, unless otherwise stated, the use of "or" means "and / or". In addition, the use of the term "including" and other forms is non-limiting.

[0019] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] According to one aspect of the present invention, there is provided a Chinese medicine composition for antibacterial and anti-exudative treatment of acute wounds, which comprises, per 1000 parts by weight: 200-400 parts of Scutellaria baicalensis, 200-400 parts of Phellodendron chinense, 200-400 parts of Catechu, and 100-200 parts of Borneol, which are dissolved in 80% ethanol and quantified to 1000 ml.

[0021] The present invention is a complete system of "Chinese medicine for treating acute wounds such as burns and scalds" summarized by famous old Chinese medicine practitioners across the country based on clinical experience. The prescription principle is to be compatible according to the principle of monarch, minister, assistant and envoy, and the formula of the composition of the present invention is optimized and determined, so that the drugs can achieve the purpose of rigorous compatibility, excellent prescription, and treating both the symptoms and the root cause. The details are as follows: In the prescription, Scutellaria baicalensis is good at treating upper-burner diseases and symptoms, and it mainly enters the lung meridian. The lung governs the skin and fur, so Scutellaria baicalensis is the first choice for skin and fur lesions. It is good at purging excess fire, removing damp heat, stopping bleeding, removing carbuncle and furuncle, and it is the main ingredient for the wound surface dampness and toxins. Phellodendron chinense and Scutellaria baicalensis are used together, and the functions of clearing heat and drying dampness, purging fire and detoxifying are auxiliary to the main medicine and serve as the minister. Borneol can open all orifices, disperse stagnant fire, open the orifices and wake up the brain, clear heat and stop itching, reduce swelling, promote tissue regeneration and relieve pain. Catechu can promote blood circulation and relieve pain, stop bleeding and promote tissue regeneration, and absorb moisture and heal sores. The two together play the role of absorbing moisture and heal sores, reducing swelling, promoting tissue regeneration and relieving pain. The drug mechanism of the components in the formula of the present invention is as follows: Scutellaria baicalensis: purging fire and detoxifying, removing dampness and stopping bleeding. Modern pharmacological research shows that Scutellaria baicalensis is rich in flavonoids, polysaccharides, volatile oils and trace elements, etc., and has good hemostatic, antibacterial, anti-inflammatory, antioxidant, analgesic and antipyretic effects.

[0022] Phellodendron chinense: clears away heat and dampness, purges fire and detoxifies. Modern pharmacological studies have shown that the alkaloids in Phellodendron chinense can fight against bacteria, fungi, viruses and other pathogenic microorganisms; inhibit cellular immune responses, and have anti-ulcer effects.

[0023] Borneol: Clears away heat and relieves itching, reduces swelling, promotes tissue regeneration and relieves pain. Borneol can be absorbed through the eyes, skin, oral mucosa, nasal mucosa, rectum and other parts of the body. It is one of the important medicines in traditional Chinese medicine surgery. It has good antibacterial, antifungal, antiviral, anti-inflammatory and analgesic effects.

[0024] Catechu: promotes blood circulation and relieves pain, stops bleeding and promotes tissue regeneration, and absorbs moisture and heals sores. Modern pharmacological studies have shown that tannin, the active ingredient in catechu, can be astringent, reduce swelling, and relieve pain; at the same time, the quercetin contained in catechu has the effect of inhibiting pathogenic microorganisms, and has a good inhibitory effect on Gram-positive cocci, Gram-negative bacilli, and fungi, can reduce secretions, keep the wound surface dry, and has no obvious pain stimulation to the wound surface. It is convenient and inexpensive, making it the best choice for treating acute burn wounds.

[0025] It should be noted that the weight of the above components is calculated based on crude drugs. Each portion can be 1g, or kilograms or tons. If grams are used as units, the formula composition can be made into 1000 doses of pharmaceutical preparations. The 1000 doses refer to the finished pharmaceutical preparations, such as 1000g of tinctures, etc., which can also be made into large packages as tinctures, such as 100-500 bags, specifically 100 bags, 125 bags, 200 bags, 250 bags, 500 bags, etc., each bag can be used as a single dose.

[0026] The above composition can be made into a preparation with a dosage of 50-1000 times. For example, as a tincture, 125 bags can be made, 1-2 bags can be taken each time, and a total of 62.5-125 times can be taken.

[0027] The above composition is based on weight ratio, which can be increased or decreased according to the corresponding ratio during production. For example, large-scale production can be based on kilograms or tons, and small-scale production can also be based on milligrams. The weight can be increased or decreased, but the weight ratio of the raw medicinal materials in the composition of the medicine remains unchanged.

[0028] In order to better improve the antibacterial and anti-exudation effects of the Chinese medicine combination provided by the present invention, the dosage of each component of the formula is further adjusted and optimized. Preferably, based on 1000 parts by weight, it includes: 100-300 parts of Scutellaria baicalensis, 100-300 parts of Phellodendron chinense, 100-300 parts of Catechu, and 50-150 parts of Borneol, which are dissolved in 80% ethanol and quantified to 1000 ml. More preferably, based on 1000 parts by weight, it includes: 200-400 parts of Scutellaria baicalensis, 200-400 parts of Phellodendron chinense, 200-400 parts of Catechu, and 100-300 parts of Borneol, which are dissolved in 75%-80% ethanol and quantified to 1000 ml.

[0029] Based on the beneficial effects of the Chinese medicine composition provided by the present invention, the second aspect of the present invention further provides the use of the above-mentioned Chinese medicine composition in the preparation of a pharmaceutical preparation for antibacterial and anti-exudation of acute wounds.

[0030] According to a third aspect of the present invention, there is provided a pharmaceutical preparation comprising an active ingredient and a pharmaceutically acceptable excipient; The active ingredient includes the above-mentioned Chinese medicine composition; The pharmaceutically acceptable excipients include: one or more of solvents, propellants, solubilizers, cosolvents, emulsifiers, colorants, adhesives, disintegrants, fillers, lubricants, wetting agents, osmotic pressure regulators, stabilizers, glidants, flavoring agents, preservatives, suspending agents, coating materials, fragrances, anti-adhesives, antioxidants, chelating agents, penetration enhancers, pH regulators, buffers, plasticizers, surfactants, foaming agents, defoamers, thickeners, inclusion agents, humectants, absorbents, diluents, flocculants and deflocculating agents, filter aids or release retardants, and can be selected according to actual needs.

[0031] The above-mentioned Chinese medicine composition can be prepared into any acceptable preparation form, for example, it can be, but not limited to, tincture or spray.

[0032] The present invention is further described below by way of examples. Unless otherwise specified, the materials in the examples are prepared according to existing methods or directly purchased from the market.

[0033] Example 1 This embodiment provides a Chinese medicine composition for antibacterial and anti-exudation of acute wounds, which comprises, based on 1000 parts by weight: 300 grams of Scutellaria baicalensis, 300 grams of Phellodendron amurense, 300 grams of Catechu, and 100 grams of Borneol, add alcohol to make up to 1000ml.

[0034] Example 2 This embodiment provides a Chinese medicine composition for antibacterial and anti-exudative treatment of acute wounds, which comprises, in 1000 parts by weight, 400 parts of Scutellaria baicalensis, 200 parts of Phellodendron amurense, 200 parts of Catechu, and 200 parts of Borneol, and alcohol is added to make the volume to 1000 ml.

[0035] Example 3 This embodiment provides a Chinese medicine composition for antibacterial and anti-exudative treatment of acute wounds, which comprises, in 1000 parts by weight, 300 parts of Scutellaria baicalensis, 200 parts of Phellodendron amurense, 300 parts of Catechu, and 200 parts of Borneol, and alcohol is added to make the volume to 1000 ml.

[0036] Example 4 This embodiment provides a Chinese medicine composition for antibacterial and anti-exudative treatment of acute wounds, which comprises, in 1000 parts by weight, 300 parts of Scutellaria baicalensis, 300 parts of Phellodendron amurense, 200 parts of Catechu, and 200 parts of Borneol, and alcohol is added to make the volume to 1000 ml.

[0037] Example 5 This embodiment provides a Chinese medicine composition for antibacterial and anti-exudation of acute wounds, which comprises, in 1000 parts by weight: 300 parts of Scutellaria baicalensis, 300 parts of Phellodendron amurense, 100 parts of Catechu, and 300 parts of Borneol, and alcohol is added to make the volume to 1000 ml.

[0038] Comparative Example 1 This comparative example provides a Chinese medicine composition for antibacterial and anti-exudation of acute wounds, which comprises, based on 1000 parts by weight: 300 parts of Scutellaria baicalensis, 300 parts of Phellodendron amurense, 300 parts of Catechu, and 100 parts of Borneol, and alcohol is added to make the volume to 1000 ml.

[0039] Comparative Example 2 This comparative example provides a Chinese medicine composition for antibacterial and anti-exudative treatment of acute wounds, which is different from Example 1 in that it does not contain Scutellaria baicalensis.

[0040] Comparative Example 3 This comparative example provides a Chinese medicine composition for antibacterial and anti-exudation of acute wounds, which is different from Example 1 in that it does not contain Phellodendron amurense.

[0041] Comparative Example 4 This comparative example provides a Chinese medicine composition for antibacterial and anti-exudation of acute wounds, which is different from Example 1 in that it does not contain catechu.

[0042] Comparative Example 5 This comparative example provides a Chinese medicine composition for antibacterial and anti-exudative use on acute wounds, which is different from Example 1 in that it does not contain borneol.

[0043] Experimental example Animal model preparation and treatment Twenty-four healthy SD rats, either sex, weighing 210-250 g, were purchased from the Experimental Animal Center of Anhui Medical University, with the certificate number SCXK (Wan) 2011-002. All rats were divided into three groups according to the random number table method: scald group, scald + 75% alcohol group, scald + Chinese medicine group, with 8 rats in each group (n=8).

[0044] The rats were deprived of water and food for 12 h. After shaving the back hair, they were depilated with 10% sodium sulfide solution. The rats were anesthetized by intraperitoneal injection of 10% chloral hydrate (300 mg / kg), the back hair was shaved, and the tail vein was catheterized. The back of the rats was immersed in 98℃ water for 12 s, causing a deep second-degree burn of 30% of the total body surface area (TBSA); the back of the rats in the sham injury group was shaved and immersed in 37℃ water for 12 s. Pathology confirmed deep second-degree burns: the local epidermis disappeared at the burn site, some cells in the dermis were necrotic, the blood vessels of the subcutaneous tissue were dilated and edematous, and acute and chronic inflammatory cell infiltration was seen, which was a deep second-degree burn.

[0045] 30 minutes after the rats were scalded, the positive control group was given 75% alcohol, and the Chinese medicine group was given a Chinese medicine composition on the wound surface. The dressing was changed twice a day, and sterile saline was used to wash before each dressing change. To prevent the rats from licking the wound, a hard shell collar was put on their head and neck. The rats were kept in a single cage and had free access to food and water.

[0046] Detection indicators Wound healing rate Five rats were selected from each group, and the wound healing rates at 3 days, 7 days, and 14 days were calculated. The wound healing rate was calculated by the marking and weighing method: 2 days after modeling, the burn area was taken as the burn area, and the rat wound was covered with a grid transparent paper, and the edge was drawn as a mark. The grid paper was cut and weighed to replace the wound area. Wound healing rate / % = (burn area-wound area) / burn area×100%.

[0047] The moisture content of wound tissue was measured and calculated at 3, 7, and 14 days. Rats were anesthetized and killed by cervical dislocation. About 200 mg of intact skin tissue from the wound was cut off, cleaned and weighed (W1), and oven-dried (80°C, 24 h) and weighed (W2). The moisture content of the tissue was calculated by the method of the decrease in moisture before and after tissue drying: Wound moisture content / % = (W1-W2) / W1×100%.

[0048] Statistical methods All data were analyzed using SPSS 19.0 software. The Kolmogorov-Smirnov method was used to test the normality of the measurement data, and the data were expressed as mean ± standard deviation (x ± s). One-way analysis of variance was used to compare the data between groups, and the LSD test was used to compare the data between two groups; P < 0.05 was considered statistically significant.

[0049] result 1 Effect of Chinese medicine group on wound healing rate of rats with deep second-degree burns There was no difference in wound healing rate among rats in each group on d 3 after burn; on d 7, the wound healing rate increased significantly, and compared with the model group, the positive control group, high-dose and low-dose scab-forming tincture groups were significantly different (P < 0.05); on d 14, there were statistically significant differences between each group and the model group (P < 0.05), and there were statistically significant differences between the Chinese medicine group and the positive medicine group (P < 0.05). This shows that the Chinese medicine composition can promote wound healing, and the healing effect is related to time. The wound healing effect shows an upward trend with the extension of medication time. See Table 1 for details. Table 1. Effect of Chinese herbal medicine combination on wound healing rate in rats. (x±s) Group Wound healing rate 3 days Wound healing rate 7 days Wound healing rate 14 days Burn group 11.46±0.48 32.81±3.29 61.37±3.11 Alcohol group 9.95±0.96 51.09±1.9 83.16±1.22 Chinese Medicine Group 12.05±1.16 52.09±6.41 90.11±3.21 2 Effect of Chinese herbal medicine combination on the moisture content of deep second-degree burn wounds in rats The wound water content of rats with deep second-degree burns in each group was significantly higher than that in the normal group (P < 0.05). In the alcohol group and the Chinese medicine group, the wound water content decreased on day 1. As the medication continued, the water content gradually decreased at each time point of the burn, but it was still higher than that in the normal group. The wound water content decreased significantly on days 7 and 14, and the difference was significant compared with the model group (P < 0.05), as shown in Table 2.

[0050] Table 2. Effect of Chinese herbal medicine combination on the water content of deep second-degree burn wounds in rats. (x±s) Group Wound healing rate 1 day Wound healing rate 3 days Wound healing rate 7 days Wound healing rate 14 days Burn group 84.15±1.27 81.24±1.08 78.37±1.41 70.34±2.16 Alcohol group 81.78±0.41 80.52±1.04 76.10±1.02 64.15±0.98 Chinese Medicine Group 82.77±1.09 82.02±0.67 74.29±1.26 60.25±0.75 in conclusion Administration of the Chinese medicine composition to the wounds of rats with deep second-degree scald can significantly reduce wound exudation, effectively alleviate tissue damage, and accelerate wound healing.

[0051] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A Chinese medicine composition for antibacterial and anti-exudative treatment of acute wounds, characterized in that: The invention comprises, based on 1000 parts by weight, scutellaria baicalensis, phellodendron amurense, catechu, borneol and ethanol.

2. The Chinese medicine composition according to claim 1, characterized in that The invention comprises 200-400 parts of scutellaria baicalensis, 200-400 parts of phellodendron amurense, 200-400 parts of catechu and 100-200 parts of borneol, which are dissolved in 80% ethanol and quantitatively made into 1000 ml.

3. The Chinese medicine composition according to claim 2, characterized in that: The invention comprises, based on 1000 parts by weight, 150-550 parts of scutellaria baicalensis, 150-550 parts of phellodendron amurense, 150-550 parts of catechu, and 100-250 parts of borneol, which are dissolved in 75%-80% ethanol and quantitatively adjusted to 1000 ml.

4. Use of the Chinese medicine composition according to any one of claims 1 to 3 in the preparation of a pharmaceutical preparation for antibacterial and anti-exudative treatment of acute wounds.

5. A pharmaceutical preparation, characterized in that The pharmaceutical preparation comprises an active ingredient and pharmaceutically acceptable excipients; The active ingredient comprises the Chinese medicine composition according to any one of claims 1 to 3.

6. The pharmaceutical preparation according to claim 5, characterized in that The dosage form of the pharmaceutical preparation includes tincture or spray.

7. The method for preparing the pharmaceutical preparation according to claim 5 or 6, characterized in that: The method comprises the steps of processing the components of scutellaria baicalensis, phellodendron amurense and catechu into coarse powder, soaking them with 250 ml of 80% ethanol in a 100,000-class clean area, mixing them evenly, sealing them and leaving them for 4 hours, placing them into a percolator with filter material on the bottom, soaking them with ethanol for 48-72 hours, preparing them according to the percolation method (percolation speed is 4 ml / min), collecting 900 ml of the percolation liquid, adding borneol to dissolve it, adding ethanol to make it into a full volume of 1000 ml, and sterilizing them with circulating steam at 100°C for 30 minutes.

8. The preparation method according to claim 7, characterized in that: You can also use the traditional soaking method, but it takes more than seven days. You can also heat and reflux in a distillation kettle, which only takes about 4 hours. After the extraction is completed, filter and wash the filter residue with 95% ethanol or aqueous ethanol to obtain it.

9. The preparation method according to claim 7, characterized in that: If 95% ethanol is used as the solvent, the filtrate obtained can be used directly in clinical practice. However, due to the high concentration of ethanol, it is very irritating to the damaged wound surface and the patient will feel severe pain. Therefore, distilled water should be added to the wound fluid to control the ethanol concentration at around 40-60%. The resulting liquid medicine can then be directly filled into a spray bottle to obtain a spray.