Recombinant engineering bacterium for producing lumbrukinase and application thereof
By expressing lumonkinase F238 in recombinant engineered bacteria and using CRISPR-Cas9 gene editing technology to transform the strain, the problems of low yield and insufficient activity of lumonkinase in the prior art were solved, and efficient expression and high activity of lumonkinase production were achieved, laying the foundation for its application in clinical treatment.
CN119685180APending Publication Date: 2025-03-25济宁华能制药厂有限公司
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Patent Information
- Application Number
- CN202411502910.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-25
- Publication Date
- 2025-03-25
AI Technical Summary
Technical Problem
The prior art is difficult to effectively produce high-purity lumonkinase, which leads to limited application in clinical treatment and is difficult to achieve industrial production in low yields.
Method used
Lumbinase F238 is expressed in recombinant engineered bacteria and targeted knockout of TDA3 and KRE9 genes, and/or overexpressing the SGA1 gene, to improve the expression and activity of lumbinase.
Benefits of technology
It significantly improves the expression and activity of lumonkinase, which can achieve up to 235% of human lumonkinase and up to 699.6U/mL, laying the foundation for the industrial application of lumonkinase.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention provides a recombinant engineering bacterium for producing lumbrukinase and application of the recombinant engineering bacterium, and relates to the technical field of genetic engineering. According to the invention, when the lumbrukinase F238 is expressed, one or more genes in TDA3 and KRE9 genes are knocked out in a targeted manner by utilizing a CRISPR-Cas9 gene editing technology, and / or an SGA1 gene is overexpressed and integrated into a strain, so that the expression quantity of the lumbrukinase F238 is increased. According to the recombinant engineering bacterium, the yield of the lumbrukinase can be obviously increased and can reach 235% at most, meanwhile, the activity of the lumbrukinase is obviously enhanced and can reach 699.6 U / mL at most, and the recombinant engineering bacterium has a wide application prospect.
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