Anti-alopecia and antipruritic composition as well as preparation method and application thereof

Abstract

The invention discloses an anti-alopecia and antipruritic composition as well as a preparation method and application thereof, and belongs to the technical field of daily chemicals. The tea saponin and the Australian tea tree extract are compounded, so that the cleaning, antibacterial and itching relieving effects of the composition can be synergistically improved; the allantoin, the orange peel essential oil and the glacial acetic acid are compounded, so that hair can be softer, brighter and easier to comb; at least one of amino acid, polypeptide, polypeptide polyethylene glycol amino and glutelin is introduced into the composition, nutrition can be provided for skin, hair loss can be prevented, and hair growth can be promoted.

Description

Technical Field

[0001] The invention relates to the technical field of daily chemical products, and in particular to an anti-hair loss and antipruritic composition and a preparation method and application thereof. Background Art

[0002] At present, most shampoo products use chemical synthetic ingredients, such as synthetic surfactants, detergents, softeners, etc. Long-term use of shampoo products made of chemical synthetic ingredients may cause a decrease in human hair volume. Therefore, some people have begun to try to return to shampoo products made from natural plant ingredients (such as tea bran, saponins, plant ash, etc.). However, the anti-hair loss effect of such shampoo products is not obvious, the effect is slow, and it is difficult to realize productization and industrialization.

[0003] Therefore, it is very necessary to develop a composition that can prevent hair loss and relieve itching and has a quick effect. Summary of the invention

[0004] The purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide a composition that can prevent hair loss and relieve itching and has a quick effect.

[0005] To achieve the above object, the technical solution adopted by the present invention is:

[0006] In a first aspect, the present invention provides an anti-hair loss and antipruritic composition, comprising the following components in weight percentage: 0.05-0.5% tea saponin, 1-10% Australian tea tree extract, 0.1-0.4% allantoin, 0.1-1% orange peel essential oil, 0.1-1% glacial acetic acid, 0.01-0.5% additives, and the remainder water.

[0007] In the present invention, tea saponin generally has anti-inflammatory, antibacterial and skin cleansing effects. It is a natural surfactant that is widely present in many plants, especially tea trees. Tea saponin can remove grease and dirt on the skin surface, and can also relieve skin inflammation. It has a certain alleviating effect on mild skin redness and inflammatory reactions, has an inhibitory effect on many bacteria, and can be used to clean wounds to reduce the risk of wound infection.

[0008] The Australian tea tree extract is extracted from the leaves and young branches of Melaleuca alternifolia, a plant endemic to New South Wales, Australia, and is obtained by low-pressure distillation. This extract contains extracts from tea trees, water-soluble components, and juice in the plant body, and has strong antibacterial, antiviral, and antifungal capabilities; the antibacterial effect of the Australian tea tree extract is mainly reflected in the following aspects: (1) Antibacterial effect: The tea tree extract in the tea tree extract is a natural antibacterial agent that can inhibit the growth of various bacteria and fungi and is often used to treat skin infections and acne. (2) Anti-inflammatory effect: The tea tree extract can reduce discomfort reactions such as skin redness, itching, and pain, help relieve skin inflammation, and treat frostbite, pollen allergies, and other problems; (3) Cleansing effect: The Australian tea tree extract has a cleansing effect and can help improve skin problems and maintain the health of the skin. In general, the Australian tea tree extract, due to its natural ingredients and multiple functions, performs well in antibacterial, anti-inflammatory, and cleaning, and is suitable for skin care and household cleaning.

[0009] The allantoin has the effects of moisturizing, nourishing and softening the skin. Allantoin may be able to penetrate into the hair and repair the damaged parts, thus making the hair softer and smoother.

[0010] The inventors have found through research that the combination of tea saponin and Australian tea tree extract can synergistically enhance the cleaning, antibacterial and antipruritic effects of the composition; the combination of allantoin, orange peel essential oil and glacial acetic acid can make hair smoother, shinier and easier to comb.

[0011] Preferably, the anti-hair loss and antipruritic composition comprises the following components in parts by weight: 0.1-0.3% tea saponin, 5-8% Australian tea tree extract, 0.2-0.3% allantoin, 0.4-0.6% orange peel essential oil, 0.4-0.6% glacial acetic acid, 0.01-0.5% additives, and the balance water.

[0012] Preferably, the additives include tea flavonoids and tea seed oil; in the anti-hair loss and antipruritic composition, the weight percentage of the tea flavonoids is 0.05-0.1%, and the weight percentage of the tea seed oil is 0.01-0.03%.

[0013] The inventors have found through research that the introduction of tea flavonoids and tea seed oil into the composition can improve the antioxidant effect and enhance the anti-hair loss effect.

[0014] Preferably, the additive further comprises at least one of amino acids, polypeptides, polypeptide polyethylene glycol amino groups and gluten.

[0015] The inventors have found through research that introducing at least one of amino acids, polypeptides, polypeptide polyethylene glycol amino groups and gluten into the composition can not only provide nutrition for the skin, but also prevent hair loss and promote hair growth.

[0016] Further preferably, the additive comprises amino acids and gluten; in the anti-hair loss and antipruritic composition, the mass percentage of the amino acids is 0.1-0.3%, and the mass percentage of the gluten is 0.05-0.1%.

[0017] More preferably, the amino acids include the following components in parts by weight: 5 to 15 parts of L-proline, 3 to 8 parts of L-serine, 5 to 15 parts of L-alanine, 3 to 8 parts of L-arginine, 1 to 5 parts of L-histidine, 0.1 to 1 part of L-tryptophan, 5 to 15 parts of L-valine, 3 to 8 parts of L-threonine, 6 to 20 parts of L-leucine, 0.5 to 2 parts of L-methionine, 5 to 15 parts of L-isoleucine, 0.5 to 2 parts of L-phenylalanine, 5 to 15 parts of L-lysine acetate, 0.1 to 1 part of L-cysteine ​​hydrochloride, 5 to 15 parts of glycine, and 0.1 to 1 part of sodium bisulfite.

[0018] Preferably, the additive comprises polypeptide polyethylene glycol amino, and the weight percentage of the polypeptide polyethylene glycol amino in the anti-hair loss and antipruritic composition is 0.01 to 0.1%.

[0019] In a second aspect, the present invention provides a method for preparing the anti-hair loss and antipruritic composition as described in the first aspect, comprising the following steps:

[0020] The components of the anti-hair loss and anti-itching composition are mixed and then sterilized and filtered to obtain the anti-hair loss and anti-itching composition.

[0021] Preferably, the preparation method comprises the following steps:

[0022] S1. Take some water, add tea flavonoids, glutenin, tea saponin and allantoin to the water in sequence, stir at a speed of 30-60 r / min until fully dissolved, and filter to obtain solution A;

[0023] S2, adding Australian tea tree extract, orange peel essential oil, and tea seed oil to glacial acetic acid in sequence, stirring at a speed of 30 to 60 r / min until fully dissolved, to obtain solution B;

[0024] S3, adding solution B to solution A, stirring at a speed of 30-60 r / min until fully dissolved, to obtain solution C;

[0025] S4. Add the amino acid to solution C, stir at a speed of 30 to 60 r / min until fully dissolved, add the remaining water, mix, sterilize, and filter to obtain the anti-hair loss and antipruritic composition.

[0026] Preferably, the preparation method comprises the following steps:

[0027] S1. Take some water, add tea flavonoids, tea saponin and allantoin to the water in sequence, stir at a speed of 30-60 r / min until fully dissolved, and filter to obtain solution A;

[0028] S2, adding Australian tea tree extract, orange peel essential oil, and tea seed oil to glacial acetic acid in sequence, stirring at a speed of 30 to 60 r / min until fully dissolved, to obtain solution B;

[0029] S3, adding solution B to solution A, stirring at a speed of 30-60 r / min until fully dissolved, to obtain solution C;

[0030] S4. Add the polypeptide polyethylene glycol amino group to solution C, stir at a speed of 30 to 60 r / min until fully dissolved, add the remaining water, mix, sterilize, and filter to obtain the anti-hair loss and antipruritic composition.

[0031] In a third aspect, the present invention provides a use of the anti-hair loss and antipruritic composition as described in the first aspect in the preparation of daily chemical products.

[0032] Preferably, the daily chemicals include any one of shampoo, shower gel, hand soap, makeup remover, and facial cleanser.

[0033] Compared with the prior art, the present invention has the following beneficial effects:

[0034] (1) The present invention compounds tea saponin and Australian tea tree extract to synergistically enhance the cleaning, antibacterial and antipruritic effects of the composition;

[0035] (2) The present invention compounds allantoin, orange peel essential oil and glacial acetic acid to make hair smoother, glossier and easier to comb;

[0036] (3) The present invention introduces at least one of amino acids, polypeptides, polypeptide polyethylene glycol amino groups and gluten into the composition, which can not only provide nutrition for the skin but also prevent hair loss and promote hair growth. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 It is a schematic diagram of cell proliferation rate. DETAILED DESCRIPTION

[0038] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0039] The Australian tea tree extract in the examples and comparative examples is commercially available and is a distillation product of leaves and young branches of the Australian tea tree.

[0040] The amino acids in the examples and comparative examples include the following components in parts by weight: 8.0 parts of L-proline (C5H9NO2), 5.0 parts of L-serine (C3H7NO3), 7.7 parts of L-alanine (C3H7NO2), and 1.3 parts of L-arginine (C6H 14 N4O2) 6.0 parts, L-histidine (C6H9N3O2) 2.4 parts, L-tryptophan (C 11 H 12 N2O2) 0.66 parts, L-valine (C5H 11 NO2) 8.4 parts, L-threonine (C4H9NO3) 4.5 parts, L-leucine (C6H 13 NO2) 11.0 parts, L-methionine (C 15 H 11 NO2S) 1.0 part, L-isoleucine (C6H 13 NO2) 9.0 parts, L-phenylalanine (C9H 11 NO2) 1.0 part, L-lysine acetate (C6H 14 N2O2·C2H4O2) 8.6 parts, L-cysteine ​​hydrochloride (C3H7NO2S·HCl) <0.2 parts, glycine (C2H5NO2) 9.0 parts, sodium bisulfite (NaHSO3) 0.5 parts.

[0041] Unless otherwise specified, the materials used in the embodiments and comparative examples of the present invention are all commercially available raw materials, and the raw materials used in each parallel experiment are of the same kind.

[0042] Embodiments 1 to 4

[0043] The embodiment of the anti-hair loss and anti-itching composition of the present invention, the formula of the anti-hair loss and anti-itching composition is shown in Table 1, and the preparation method of the anti-hair loss and anti-itching composition is as follows:

[0044] S1. Take 60% of the formula amount of deionized water, add tea flavonoids, glutenin, tea saponin and allantoin to the water in sequence, stir at a speed of 30-60 r / min until fully dissolved, and filter through a 0.45 μm fine filter membrane to obtain solution A;

[0045] S2, adding Australian tea tree extract, orange peel essential oil, and tea seed oil to glacial acetic acid in sequence, stirring at a speed of 30 to 60 r / min until fully dissolved, to obtain solution B;

[0046] S3, adding solution B to solution A, stirring at a speed of 30-60 r / min until fully dissolved, to obtain solution C;

[0047] S4. Add the amino acid to solution C, stir at a speed of 30 to 60 r / min until fully dissolved, add the remaining deionized water, sterilize after mixing, and filter through a 0.22 μm filter membrane to obtain the anti-hair loss and antipruritic composition.

[0048] Embodiment 5-6

[0049] The embodiment of the anti-hair loss and anti-itching composition of the present invention, the formula of the anti-hair loss and anti-itching composition is shown in Table 1, and the preparation method of the anti-hair loss and anti-itching composition is as follows:

[0050] S1. Take 60% of the formula amount of deionized water, add tea flavonoids, tea saponin and allantoin to the water in sequence, stir at a speed of 30-60 r / min until fully dissolved, and filter through a 0.45 μm fine filter membrane to obtain solution A;

[0051] S2, adding Australian tea tree extract, orange peel essential oil, and tea seed oil to glacial acetic acid in sequence, stirring at a speed of 30 to 60 r / min until fully dissolved, to obtain solution B;

[0052] S3, adding solution B to solution A, stirring at a speed of 30-60 r / min until fully dissolved, to obtain solution C;

[0053] S4. Add the polypeptide polyethylene glycol amino group to solution C, stir at a speed of 30 to 60 r / min until fully dissolved, add the remaining deionized water, sterilize after mixing, and filter through a 0.22 μm filter membrane to obtain the anti-hair loss and antipruritic composition.

[0054] Comparative Examples 1 to 6

[0055] The difference between the comparative examples of the anti-hair loss and anti-itching compositions of the present invention and Example 1 is that the formulas of the anti-hair loss and anti-itching compositions in Comparative Examples 1 to 6 are shown in Table 2.

[0056] Table 1 (Unit: weight percentage, %)

[0057]

[0058]

[0059] Table 2 (Unit: weight percentage, %)

[0060]

[0061] Effect Example 1

[0062] Dermal papilla mesenchymal cells, also called DP cells (DPCs), are located at the base of hair follicles. Dermal papilla mesenchymal cells mediate signal interactions between the hair follicle dermis and epidermis through autocrine or paracrine cytokines, and play a leading role in regulating hair growth.

[0063] The MTT method was used to detect the effects of the compositions obtained in the above examples and comparative examples on the proliferation of HDPCs cells. The detection method is as follows:

[0064] The human DPCs stored in liquid nitrogen were taken out, melted in a 37°C water bath, and then transferred to a T-25 cell culture flask and cultured in an incubator at 37°C and 5% CO2 for 12 hours. After 4 hours of adhesion, the cells were replaced with fresh complete culture medium. The cell adhesion and density were observed using a biological microscope. The cells were adhered but the confluence was less than 80%, and there were no other abnormalities. Aspirate the original culture medium in the culture flask until 5-10 mL remains. When the confluence exceeds 80%, subculture can be performed; during subculture, all the liquid in the bottle needs to be aspirated, 3-5 mL of PBS solution needs to be added, and the cell growth surface needs to be carefully rinsed and discarded; add 0.5-1 mL of trypsin containing 0.25% EDTA, digest the cells at 37°C for 2 minutes, shake the culture flask slowly to make the cells fall off from the bottom of the flask, and quickly add 3-5 mL of DMEM complete culture medium to terminate the digestion; use a sterile pipette to carefully blow the cells on the wall of the flask until they are all dispersed in the liquid, transfer them to a sterile centrifuge tube, centrifuge at 1000 rpm for 5 minutes, carefully remove the bubbles and liquid, add fresh complete culture medium to redisperse the cells, and after subculture, place the culture flask in a 37°C, 5% CO2 and saturated humidity incubator for culture.

[0065] MTT method to measure cell proliferation: digest logarithmic growth phase cells and count with a hemocytometer. Inoculate 1×104 cells per well in a 96-well cell culture plate, culture in an incubator at 37°C, 5% CO2 and saturated humidity for 24 hours, discard the supernatant, and rinse once with PBS solution; use a DMEM solution containing 10 μg / mL minoxidil as a positive control, and add a DMEM solution containing the composition to the sample well by a two-fold dilution method (the mass percentage of the composition in the DMEM solution containing the composition is 0.1%), with 3 parallels in each group, and the source of the composition in each group is as follows Figure 1 As shown; after culturing for 24 hours, the liquid in the wells was aspirated, and each well was washed twice with 3-5 mL PBS solution, and 100 μL of MTT culture solution with a concentration of 0.5 mg / mL was added to each well, and the cells were cultured in an incubator for 4 hours; the culture solution in the wells was carefully aspirated, and 100 mL of dimethyl sulfoxide was added to each well, and the cells were placed on a shaker and shaken for 5 minutes to fully dissolve the crystals. The optical density of each well was measured at OD490 nm by an enzyme-linked immunosorbent assay, and the DPCs cell proliferation rate was calculated according to the following formula:

[0066] DPCs cell proliferation rate = (sample group OD - reagent group OD) / (blank control group OD - reagent group OD) × 100%.

[0067] Graphpad prism statistical software was used for statistics, and P < 0.05 was considered statistically significant.

[0068] The results are as follows Figure 1 shown.

[0069] from Figure 1 It can be seen that the composition provided by the present invention can effectively promote the proliferation of DPCs cells.

[0070] Effect Example 2

[0071] 130 subjects were recruited and screened, half male and half female, male aged 26 to 51 years old, female aged 20 to 56 years old. They were randomly divided into 13 groups, 10 people in each group, and the compositions obtained in the examples and comparative examples were used respectively, and the use method was as follows:

[0072] (1) Soaking: Take 1 part of the anti-hair loss and anti-itching plant shampoo, dilute it with 4 to 8 parts of warm water and put it in a basin, wet the hair and scalp, and repeatedly pour and scrub for 5 to 10 minutes;

[0073] (2) Rinse: Take 1 part of this anti-hair loss and anti-itching botanical shampoo, dilute it with 8-15 parts of warm water and rinse thoroughly;

[0074] Men should wash their hair every 3 days, and women should wash their hair every 5 days.

[0075] The screening method for subjects is as follows:

[0076] The inclusion criteria for the subjects were to be tested in accordance with the specific requirements of the cosmetics anti-hair loss efficacy test in the "Technical Specifications for Safety of Cosmetics" (2015 edition). The subjects were screened and the number of hair loss counted by the 60-time combing method was greater than 10 as qualified subjects; qualified subjects underwent a 2-week washout period; after the washout period, the 60-time combing method was performed again, and only those with hair loss counts greater than 10 could enter the formal test. The subjects selected for the formal test were divided into the test product group and the control product group according to the stratified random method.

[0077] Hair loss count: Before use, 4 weeks, 8 weeks and 12 weeks of use, trained staff combed the subjects' hair 60 times using the combing method, counted the lost hair, and recorded the hair loss counts of each group of subjects. The hair loss count difference was calculated according to the following formula: Hair loss count difference = hair loss count at a certain point after use - hair loss count before use.

[0078] Local hair density: A haircut area of ​​at least 1.5 cm × 1.5 cm (temporal side of the top of the head) was fixed on the subject's head and positioned properly. During each visit, the haircut area was kept consistent and the hair was cut to a residual length of no more than 1 mm. During the image acquisition process, the operator needed to put the subject in a comfortable position and place the dermatoscope in the center of the haircut area to capture local hair images. During the shooting, the dermatoscope lens was completely in contact with the scalp and kept vertical, and the clarity of the captured image was checked. Image analysis software or manual counting methods were used to count the number and density of local hair, and the number and density of local hair of each group of subjects were recorded. The difference in local hair density was calculated according to the following formula: local hair density difference = local hair density value at a certain point after use - local hair density value before use.

[0079] The results are shown in Table 3.

[0080] Table 3

[0081]

[0082]

[0083] As can be seen from Table 4, after using the anti-hair loss and antipruritic composition provided by the present invention for 8 weeks, the difference in hair loss counts was significantly reduced, and the difference in local hair density was significantly increased, which shows that the composition provided by the present invention can have excellent anti-hair loss effect.

[0084] It can be seen from Example 6 and Comparative Examples 1 to 3 that the combination of allantoin, orange peel essential oil and glacial acetic acid in the composition of the present invention can not only effectively improve the softness of hair, but also reduce the amount of hair loss in the initial use (4 weeks), regulate the scalp environment, promote hair growth, and increase local hair density.

[0085] It can be seen from Example 6 and Comparative Examples 4 to 5 that the introduction of tea flavonoids and Australian tea tree extract can not only reduce hair loss, but also effectively promote hair growth and increase local hair density.

[0086] It can be seen from Example 2, Example 6 and Comparative Example 6 that the present invention introduces at least one of amino acids, polypeptides, polypeptide polyethylene glycol amino groups and gluten into the composition. Long-term use of the composition of the present invention can effectively improve the anti-hair loss effect, effectively promote hair growth and increase hair density.

[0087] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. An anti-hair loss and antipruritic composition, characterized in that: The invention comprises the following components in weight percentage: 0.05-0.5% of tea saponin, 1-10% of Australian tea tree extract, 0.1-0.4% of allantoin, 0.1-1% of orange peel essential oil, 0.1-1% of glacial acetic acid, 0.01-0.5% of additives and the balance of water.

2. The anti-hair loss and anti-itching composition according to claim 1, characterized in that: The invention comprises the following components in parts by weight: 0.1-0.3% of tea saponin, 5-8% of Australian tea tree extract, 0.2-0.3% of allantoin, 0.4-0.6% of orange peel essential oil, 0.4-0.6% of glacial acetic acid, 0.01-0.5% of additives and the balance of water.

3. The anti-hair loss and anti-itching composition according to claim 1, characterized in that: The additives include tea flavonoids and tea seed oil. In the anti-hair loss and antipruritic composition, the weight percentage of the tea flavonoids is 0.05-0.1%, and the weight percentage of the tea seed oil is 0.01-0.03%.

4. The anti-hair loss and anti-itching composition according to claim 1, characterized in that: The additive further comprises at least one of amino acids, polypeptides, polypeptide polyethylene glycol amino groups and gluten.

5. The anti-hair loss and anti-itching composition according to claim 4, characterized in that: The additives include amino acids and gluten; in the anti-hair loss and anti-itching composition, the mass percentage of the amino acids is 0.1-0.3%, and the mass percentage of the gluten is 0.05-0.1%.

6. The anti-hair loss and anti-itching composition according to claim 5, characterized in that: The amino acids include the following components in parts by weight: 5 to 15 parts of L-proline, 3 to 8 parts of L-serine, 5 to 15 parts of L-alanine, 3 to 8 parts of L-arginine, 1 to 5 parts of L-histidine, 0.1 to 1 part of L-tryptophan, 5 to 15 parts of L-valine, 3 to 8 parts of L-threonine, 6 to 20 parts of L-leucine, 0.5 to 2 parts of L-methionine, 5 to 15 parts of L-isoleucine, 0.5 to 2 parts of L-phenylalanine, 5 to 15 parts of L-lysine acetate, 0.1 to 1 part of L-cysteine ​​hydrochloride, 5 to 15 parts of glycine, and 0.1 to 1 part of sodium bisulfite.

7. The anti-hair loss and anti-itching composition according to claim 1, characterized in that: The additive comprises polypeptide polyethylene glycol amino, and the weight percentage of the polypeptide polyethylene glycol amino in the anti-loss and antipruritic composition is 0.01-0.1%.

8. A method for preparing the anti-hair loss and anti-itching composition according to any one of claims 1 to 7, characterized in that: The steps include: The components of the anti-hair loss and anti-itching composition are mixed and then sterilized and filtered to obtain the anti-hair loss and anti-itching composition.

9. Use of the anti-hair loss and anti-itching composition according to any one of claims 1 to 7 in the preparation of daily chemicals.

10. The use according to claim 9, characterized in that The daily chemicals include any one of shampoo, shower gel, hand soap, makeup remover, and facial cleanser.

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