Medicago sativa MsKTI3 gene and application of medicago sativa MsKTI3 gene in improvement of plant salt tolerance

By screening and cloning the salt stress response gene MsKTI3 of alfalfa and overexpressing it, the problem of growth restriction of alfalfa under salt stress was solved, significantly improving the salt tolerance and antioxidant ability of plants, and providing key resources for its salt tolerance molecular improvement.

CN119842737AActive Publication Date: 2025-04-18INSTITUTE OF ANIMAL SCIENCES OF CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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Patent Information

Application Number
CN202510131999.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-02-06
Publication Date
2025-04-18
Estimated Expiration
2045-02-06

AI Technical Summary

Technical Problem

Alfalfa is limited in growth under salt stress, resulting in reduced yield and impaired ecosystem stability. The existing technology has not yet effectively explored the function of its salt-tolerant gene.

Method used

The response candidate gene MsKTI3 was screened and cloned by alfalfa salt stress, and the recombinant vector was constructed for overexpression, thereby improving the salt tolerance of the plant.

Benefits of technology

Successfully overexpressing the MsKTI3 gene in Arabidopsis and alfalfa significantly improved the germination rate, growth performance and antioxidant ability of plants under high salt conditions, and provided candidate gene resources for the improvement of salt-tolerant molecules of alfalfa.

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Abstract

The invention discloses a medicago sativa MsKTI3 gene and application of the medicago sativa MsKTI3 gene in improving the salt tolerance of plants, a candidate gene MsKTI3 related to the salt tolerance of medicago sativa is screened out through transcriptome sequencing analysis, the MsKTI3 is cloned, the medicago sativa and arabidopsis thaliana are over-expressed, and the functions of the gene are identified. According to the invention, a foundation is laid for deeply researching functions and a salt-tolerant regulation mechanism of MsKTI3, and a candidate gene resource is also provided for improving salt-tolerant molecules of alfalfa.
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