RPA-CRISPR / Cas12a-based sequence combination for rapidly detecting Philospora maydis and application of RPA-CRISPR / Cas12a-based sequence combination
Through RPA-CRISPR/Cas12a technology, specific primers and crRNA sequences are designed and combined with signal reporter molecules, high sensitivity and high specificity rapid detection of corn spot bacteria is achieved, solving the detection problems in the existing technology, and is suitable for real-time diagnosis in the field.
CN120272641APending Publication Date: 2025-07-08HENAN INST OF SCI & TECH
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Patent Information
- Application Number
- CN202510703782.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-29
- Publication Date
- 2025-07-08
AI Technical Summary
Technical Problem
The prior art has not yet provided a high sensitivity and high specificity method to quickly detect corn spot bacteria, and it is difficult to achieve early warning and precise prevention and control.
Method used
RPA-CRISPR/Cas12a technology is used to design specific primers and crRNA sequences, combine signal reporter molecules, and quickly detect vermisporidium umbilical cords by fluorescence quantitative PCR instrument or lateral flow chromatography test strips.
Benefits of technology
It realizes simple and fast detection of corn spot bacteria under constant temperature conditions, avoids aerosol pollution, improves the specificity and sensitivity of the detection, and is suitable for real-time diagnosis in the field.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention belongs to the technical field of biological detection, and particularly relates to a sequence combination for rapidly detecting Paphanerospora maydis based on RPA-CRISPR / Cas12a and application of the sequence combination. The invention relates to a sequence combination for rapidly detecting Philospora maydis based on RPA-CRISPR / Cas12a, which is characterized in that the sequence combination comprises RPA (recombinase polymerase amplification) primers Bm-F3 / Bm-R3 and Bm-crRNA (complementary ribosomal ribonucleic acid) sequences. The specific RPA primer is designed for the bipolaris maydis which is high in separation frequency, wide in distribution range and serious in harm, and field visual rapid detection of the bipolaris maydis can be achieved by combining recombinase polymerase amplification with a CRISPR / Cas12a detection technology. The technology is easy and convenient to operate, reaction can be carried out under the constant-temperature condition, expensive instruments are not needed, and detection time is short. Aerosol pollution caused by secondary cover opening can also be avoided by using a one-tube method for detection, meanwhile, the specificity and sensitivity of RPA detection can be improved by using a CRISPR / Cas12a system, and an efficient and reliable detection scheme can be provided for field monitoring of the Philospora maydis.
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