PhiF* expression cassette, expression system and application of phiF* expression cassette in xanthomonas campestris

By constructing the phiF* expression frame and expression system, the lack of expression elements in the yeast cyperus phytonus sausage was solved, efficient induction of expression and regulation was achieved, and the basis for genetically engineered strains was provided.

CN120464660APending Publication Date: 2025-08-12ORDOS ZHONGXUAN BIOCHEM
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Patent Information

Application Number
CN202510509563.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-04-22
Publication Date
2025-08-12

AI Technical Summary

Technical Problem

The lack of expression elements suitable for oxalmonas phytonutrinosa in the prior art, resulting in the inducible expression system being unable to be effectively applied to the strain.

Method used

The phiF* expression frame and expression system were constructed, including the repressor gene phiFR*, the constitutive promoter Pj23119 and the RBS sequence. The expression of the target gene was induced in the wild plasmid pBBRMCS-2 vector using the commercial plasmid pBBRMCS-2 vector.

Benefits of technology

It has achieved efficient induction of expression in wild rapeseed Xanthomonas, and the induction magnification ratio has been increased to 4.75 times, the expression intensity has been improved, the leakage is low, and the regulation is rigorous, providing the basis for the construction of genetically engineered strains.

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Abstract

The invention relates to the technical field of gene engineering, in particular to a phiF * expression cassette, an expression system and application of the phiF * expression cassette in xanthomonas campestris. The phiF * expression cassette is composed of a constitutive promoter Pj23119, an RBS sequence and a repressor protein gene phiFR *; the nucleotide sequence and the amino acid sequence of the repressor protein gene phiFR * are respectively shown as SEQ ID NO. 1 and SEQ ID NO. 2. According to the invention, a phiF * frame and a phiF * expression system which can be used in xanthomonas campestris are constructed, and a certain basis is provided for construction of xanthomonas campestris genetic engineering strains.
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