Primers, kit and method for identifying pelteobagrus fulvidraco, pelteobagrus vachelli, leiocassis longirostris and hybrids thereof

By designing specific primers and kits, combined with PCR amplification and gel electrophoresis, the identification problems of yellow catfish, Varnaria and long-squid and their hybrids were solved, and efficient and accurate species identification was achieved, supporting aquatic breeding management.

CN120485385AActive Publication Date: 2025-08-15HUAZHONG AGRI UNIV
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Patent Information

Application Number
CN202510636205.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-05-16
Publication Date
2025-08-15
Estimated Expiration
2045-05-16

AI Technical Summary

Technical Problem

The prior art is difficult to quickly and accurately identify yellow catfish, Varnaria and squid and their hybrids, resulting in confusion in breeding management and mixed germplasm resources.

Method used

Design specific primers and kits, combine with agarose gel electrophoresis technology through PCR amplification, use fin strip microscopes to perform DNA identification, and judge species category based on the electrophoretic band position.

Benefits of technology

It has achieved high-precision, low-cost and simple operation species identification, and is suitable for aquatic breeding management during the seedling and breeding stages.

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Abstract

The invention relates to the technical field of biology, in particular to primers, a kit and a method for identifying pelteobagrus fulvidraco, pelteobagrus vachelli, leiocassis longirostris and hybrids of the pelteobagrus fulvidraco, the pelteobagrus vachelli, the leiocassis longirostris and the hybrids of the pelteobagrus fulvidraco and the leiocassis longirostris. According to the method, after DNA extraction is carried out on a fin ray trace sample, species identification can be completed through combination of PCR amplification and an agarose gel electrophoresis technology, the accuracy of a detection result is high, the genetic marker specificity is high, the method is not limited by the growth condition of fish bodies, sampling identification can be carried out in all breeding stages, and an important technical support is provided for aquatic product breeding management.
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