Cod collagen peptide eye cream and preparation method thereof

Abstract

The invention relates to the technical field of cosmetics, and particularly discloses cod collagen peptide eye cream and a preparation method thereof. According to the eye cream, cod collagen peptide with small molecular weight is used as a core active component, and a stable O / W type emulsification system is formed through a single factor and orthogonal experiment optimization formula. The eye cream has obvious effects in the aspects of moisture retention, wrinkle resistance, skin brightening and the like. The product is suitable for improving the problems of dry, loose and wrinkled skin around eyes, and the safety of the product meets the standard requirements of cosmetics.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics and preparation methods thereof, and in particular to a cod collagen peptide eye cream and a preparation method thereof. Background Art

[0002] As the skin ages, the skin around the eyes, with its thin stratum corneum and fewer sebaceous glands, is more susceptible to fine lines, sagging, and dryness. Collagen, the skin's primary structural protein, is a key factor in skin aging. Traditional animal-derived collagen, due to its large molecular weight, has difficulty penetrating the skin barrier, resulting in an absorption rate of less than 10%, and carries the risk of pathogen contamination.

[0003] Cod collagen peptides are made from deep-sea cod, using enzymatic hydrolysis to break down the peptides into bioactive small peptides with a molecular weight below 1000 Daltons. They are composed of repeating amino acid fragments (GXY)n. G stands for glycine (Gly), which accounts for approximately 30% of the amino acid content of fish collagen; while X and Y are typically proline (Pro) and hydroxyproline (Hyp), which together account for approximately 25% of the amino acid content. Oral consumption of cod collagen peptides has been shown to enhance beauty, boost immunity, promote fat metabolism, protect the liver, and promote wound healing. Cod collagen peptides also show good transdermal absorption effect (Chai HJ, Li JH, Huang HN, et al. Effects of Sizes and Conformations of Fish-Scale Collagen Peptides on Facial Skin Qualities and Transdermal Penetration Efficiency [J]. Journal of Biomedicine and Biotechnology, 2010, 2010: 1-9.), however, there have been no reports on their use in topical eye creams.

[0004] The present invention develops an eye cream preparation with cod collagen peptide as the core, which has both high stability and efficacy, and provides a new eye cream product option for the market. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to address the deficiencies of the existing technology and provide a cod collagen peptide eye cream and a preparation method thereof.

[0006] In order to achieve the purpose of the present invention, the following technical means are specifically adopted:

[0007] A cod collagen peptide eye cream, characterized by comprising the following components by mass percentage:

[0008] Core active ingredient: cod collagen peptide 2% to 10%;

[0009] Emulsification stabilization system: sodium lauroyl glutamate 1%-5%, Tween 80 2%-4%, carbomer 940 0.5%-1.5%;

[0010] Moisturizing matrix components: glycerin 3% to 5%, synthetic squalane 1% to 5%, jojoba oil 1% to 5%;

[0011] Functional auxiliary ingredients: sodium alginate 0.2% to 0.5%, propylene glycol 1% to 3%, disodium EDTA 0.2% to 0.5%, vitamin C 0.2% to 0.5%, rose essence 0.5% to 1.5%;

[0012] pH regulator: triethanolamine 0.3% to 0.7%;

[0013] Solvent: Deionized water was made up to 100%.

[0014] The cod collagen peptide eye cream is characterized in that the protein content of the cod collagen peptide is ≥90% (dry basis), and the hydroxyproline content is ≥8% (dry basis); and the pH value of the eye cream is 5.5-6.5.

[0015] The preparation method of the cod collagen peptide eye cream is characterized by comprising the following steps: (a) mixing synthetic squalane, sodium lauroyl glutamate, Tween 80, and jojoba oil, and heating and stirring at 75-80° C. until uniform; (b) swelling carbomer 940 in water, adding glycerol, sodium alginate, triethanolamine, propylene glycol, and part of water, and heating and stirring at 75-80° C. until uniform; (c) slowly adding the phase obtained in step (b) to the phase obtained in step (a), stirring and mixing at 75-80° C., and cooling to 35-45° C.; and (d) adding cod collagen peptide, disodium EDTA, vitamin C, rose essence, and remaining water, and homogenizing and emulsifying.

[0016] The cod collagen peptide eye cream is characterized in that it has the following effects: (a) increasing the moisture content of the skin around the eyes; (b) improving skin elasticity and firmness; and (c) brightening the skin tone around the eyes.

[0017] The cod collagen peptide eye cream is characterized in that it is suitable for: (a) rough and tight skin around the eyes caused by dryness; (b) fine lines and sagging caused by aging or photoaging; (c) dull skin around the eyes caused by staying up late, fatigue, etc.

[0018] The cod collagen peptide described in the present invention is powdery, free of lumps, white to light yellow, free of external visible impurities, with a protein content (on a dry basis) of ≥90%, a hydroxyproline content (on a dry basis) of ≥8%, a proportion of protein hydrolysates with a peptide relative molecular mass of less than 500 of ≥95%, a moisture content of ≤7%, and an ash content of ≤2%.

[0019] Beneficial effects

[0020] Beneficial effects of the present invention:

[0021] The cod collagen peptide eye cream developed by the present invention has good stability, pleasant fragrance, and excellent moisturizing performance. It can improve the moisture, elasticity and whiteness of the eye skin of the user and reduce wrinkles. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 For cod collagen peptide eye cream clearance rate.

[0023] Figure 2 is the clearance rate of blank matrix eye cream. DETAILED DESCRIPTION

[0024] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. The following description of at least one exemplary embodiment is actually only illustrative and is in no way intended to limit the present invention and its application or use. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0025] Description of the cod collagen peptide used in the present invention: The manufacturer is Jiaxing Newdikang Biotechnology Co., Ltd., the product batch number is HS2410308, the protein content (on a dry basis) is 99.3%, the hydroxyproline content (on a dry basis) is 11.7%, the moisture content is 6.7%, the ash content is 0.7%, and the microbial limit is qualified.

[0026] Example 1 Screening of cod collagen peptide eye cream composition

[0027] 1. Preliminary formulation of prescription

[0028] Based on the general composition of O / W eye cream and the physical and chemical properties of cod collagen peptide, the preliminary formula is shown in Table 1.

[0029] Table 1 Preliminary prescription

[0030]

[0031] 2. Proposed preparation method

[0032] (1) Weigh all the raw materials in phase A separately, place them in a clean beaker, place them in a magnetic stirrer, heat them in a water bath at 75-80°C, and stir them magnetically for 15 minutes until they become transparent and uniform. Keep warm to prepare component A.

[0033] (2) Weigh Carbomer 940 from Phase B, dissolve it completely in water beforehand, then add the other materials from Phase B, heat in a water bath at 75-80°C, and stir magnetically for 15 minutes until the mixture becomes transparent and uniform. Keep warm to prepare this component B.

[0034] (3) Slowly pour phase B into phase A, place in a magnetic stirrer in a 75-80°C water bath and stir for 15 minutes, then cool to room temperature;

[0035] (4) After the mixture of phases A and B is cooled to 35-45°C, all the materials in phase C are added and homogenized at 3000 rpm for 1-2 minutes until completely dispersed.

[0036] 3. The calculation and measurement methods of some evaluation indicators are as follows

[0037] 3.1 Sensory evaluation and stability determination

[0038] Ten volunteers aged 18 to 60 with healthy skin were randomly selected to undergo a sensory evaluation of the eye cream under the same conditions. The specific evaluation criteria are shown in Table 2 below.

[0039] Table 2 Eye cream sensory index scoring standard

[0040] Serial number Evaluation indicators Feature Description Scoring Criteria 1 Appearance status The paste should be fine and uniform 0-9 2 viscosity The stickiness of the product after it is completely absorbed into the skin 0-9 3 Ductility How easy is the product to spread during application and how easy is it to push on the skin? 0-9 4 Lubricity Feel how easily your fingers glide across your skin after the product is fully absorbed 0-9

[0041] 4. Single factor screening of prescription composition

[0042] 4.1 Investigation of the dosage of synthetic squalane

[0043] The formulations were tested using 1%, 3%, and 5% synthetic squalane, respectively. The amount of water in Phase C was adjusted to maintain a 100% formulation. Samples were prepared using the proposed method, and the amount of synthetic squalane was screened using sensory evaluation. The experimental results are shown in Table 3 below.

[0044] Table 3 Single factor investigation results of synthetic squalane dosage

[0045] Addition amount (%) Appearance Rating Viscosity score Scalability score Lubricity score Total score 1 7 6 6 6 25 3 8 7 8 8 32 5 8 5 9 7 29

[0046] The results in the table above show that the overall scores for synthetic squalane formulations at 1%, 3%, and 5% were all good (≥24 points), indicating that synthetic squalane formulations at 1-5% were all qualified. It was also noted that the 3% synthetic squalane formulation had the highest score, so this formulation was temporarily selected for subsequent screening studies.

[0047] 4.2 Investigation of glycerol dosage

[0048] A 3% synthetic squalane dosage was selected for the formulation, and glycerin dosages of 3%, 4%, and 5% were investigated. The water content in Phase C was adjusted to maintain a 100% glycerin dosage. Samples were prepared using the proposed method, and the glycerin dosage was screened using sensory evaluation. The experimental results are shown in Table 4 below.

[0049] Table 4 Results of investigation on glycerol dosage

[0050] Addition amount (%) Appearance Rating Viscosity score Scalability score Lubricity score Total score 3 7 6 6 6 25 4 8 7 8 8 31 5 7 9 7 7 30

[0051] The results in the table above show that the overall scores for 3%, 4%, and 5% glycerol prescriptions were all good (≥24 points), indicating that 3% to 5% glycerol prescriptions were all acceptable. It was also noted that the 4% glycerol prescription had the highest score, so this was temporarily selected for subsequent screening studies.

[0052] 4.3 Study on the dosage of sodium lauroyl glutamate

[0053] The formulation used synthetic squalane at 3% and glycerin at 4%. Sodium lauroyl glutamate was tested at 1%, 3%, and 5% concentrations. The water content in Phase C was adjusted to maintain a 100% concentration. Samples were prepared using the proposed method, and the sodium lauroyl glutamate concentration was screened using sensory evaluation. The results are shown in Table 5 below.

[0054] Table 5 Results of the investigation on the dosage of sodium lauroyl glutamate

[0055] Addition amount (%) Appearance Rating Viscosity score Scalability score Lubricity score Total score 1 6 6 6 6 24 3 8 7 8 8 31 5 7 5 9 6 27

[0056] The results in the table above show that the overall scores for sodium lauroyl glutamate at 1%, 3%, and 5% were all good (≥24 points), indicating that sodium lauroyl glutamate dosages of 1-5% were all acceptable. It was also noted that the highest score was achieved at a 3% sodium lauroyl glutamate dosage, so a 3% sodium lauroyl glutamate dosage was temporarily selected for subsequent screening studies.

[0057] 4.4 Study on the dosage of Tween 80

[0058] The formulation used synthetic squalane at 3%, glycerin at 4%, and sodium lauroyl glutamate at 3%. Tween 80 was tested at 2%, 3%, and 4% levels, respectively. The amount of water in Phase C was adjusted to maintain a 100% Tween 80 level. Samples were prepared using the proposed method, and the Tween 80 level was screened using sensory evaluation. The results are shown in Table 6 below.

[0059] Table 6 Results of the investigation on the dosage of Tween 80

[0060] Addition amount (%) Appearance Rating Viscosity score Scalability score Lubricity score Total score 2 6 6 7 7 26 3 8 7 8 7 30 4 8 5 8 7 28

[0061] The results in the table above show that the overall scores for Tween 80 at 2%, 3%, and 4% dosages were all good (≥24 points), indicating that Tween 80 dosages of 2-4% are all acceptable. It was also noted that the 3% Tween 80 dosage achieved the highest score, so this dosage was temporarily selected for subsequent screening studies.

[0062] 4.5 Study on the dosage of jojoba oil prescription

[0063] The dosage of synthetic squalane in the formulation was selected to be 3%, the dosage of glycerin was selected to be 4%, the dosage of sodium lauroyl glutamate was selected to be 3%, and the dosage of Tween 80 was selected to be 3%. The dosage of jojoba oil was examined at 1%, 3%, and 5%, respectively. The dosage of water in phase C was adjusted to maintain 100% of the formulation. Samples were prepared using the proposed preparation method, and the dosage of jojoba oil was screened by sensory scoring. The experimental results are shown in Table 7 below.

[0064] Table 7 Results of the investigation on the dosage of jojoba oil prescription

[0065] Addition amount (%) Appearance Rating Viscosity score Scalability score Lubricity score Total score 1 7 6 6 7 26 3 8 7 7 8 30 5 8 9 8 7 30

[0066] The results in the table above show that the overall scores for jojoba oil prescriptions at 1%, 3%, and 5% were all good (≥24 points), indicating that jojoba oil prescriptions at dosages of 1% to 5% were all qualified. It was also noted that the highest scores were achieved at 3% and 5% jojoba oil prescriptions, and the 3% jojoba oil prescription was temporarily selected for subsequent screening studies.

[0067] 4.6 Carbomer 940 Prescription Dosage Study

[0068] The formulation used synthetic squalane at 3%, glycerin at 4%, sodium lauroyl glutamate at 3%, Tween 80 at 3%, and jojoba oil at 3%. Carbomer 940 was tested at 0.5%, 1%, and 1.5% concentrations, respectively. The amount of water in Phase C was adjusted to maintain a 100% concentration. Samples were prepared using the proposed method, and the Carbomer 940 concentration was screened using sensory evaluation. The results are shown in Table 8 below.

[0069] Table 8 Carbomer 940 dosage results

[0070] Addition amount (%) Appearance Rating Viscosity score Scalability score Lubricity score Total score 0.5 6 5 6 7 24 1 7 7 7 7 28 1.5 8 9 7 6 30

[0071] As shown in the table above, the overall scores of the prescriptions were good (≥24 points) when the Carbomer 940 dosage was 0.5%, 1%, and 1.5%, indicating that the Carbomer 940 dosages of 0.5-1.5% were all qualified. It was also noted that the Carbomer 940 dosage of 1.5% had the highest score, and the Carbomer 940 dosage of 1.5% was temporarily selected for subsequent screening studies.

[0072] 4.7 Investigation of Propylene Glycol Dosage

[0073] The formulation used synthetic squalane at 3%, glycerin at 4%, sodium lauroyl glutamate at 3%, Tween 80 at 3%, jojoba oil at 3%, and carbomer 940 at 1.5%. Propylene glycol concentrations of 1%, 2%, and 3% were investigated. The amount of water in Phase C was adjusted to maintain a 100% concentration. Samples were prepared using the proposed method, and the propylene glycol concentration was screened using sensory evaluation. The results are shown in Table 9 below.

[0074] Table 9 Results of investigation on the dosage of propylene glycol prescription

[0075] Addition amount (%) Appearance Rating Viscosity score Scalability score Lubricity score Total score 1 7 7 7 6 27 2 8 7 8 7 30 3 7 9 7 6 29

[0076] The results in the table above show that the overall scores for propylene glycol prescriptions at 1%, 2%, and 3% were all good (≥24 points), indicating that prescriptions with propylene glycol dosages of 1-3% were all qualified. It was also noted that the highest score was achieved at a 2% propylene glycol dosage, and this dosage has been temporarily selected for subsequent screening studies.

[0077] 4.8 Investigation of the dosage of cod collagen peptide

[0078] The formulation contained 3% synthetic squalane, 4% glycerin, 3% sodium lauroyl glutamate, 3% Tween 80, 3% jojoba oil, 1.5% carbomer 940, and 2% propylene glycol. Codfish collagen peptide dosages of 0%, 2%, 4%, 6%, 8%, and 10% were investigated, respectively. The amount of water in phase C was adjusted to maintain a 100% formulation. Samples were prepared using the proposed preparation method, and the codfish collagen peptide dosage was screened by sensory scoring. The experimental results are shown in Table 10 below.

[0079] Table 10 Results of the investigation on the dosage of cod collagen peptide prescription

[0080]

[0081]

[0082] The results in the table above show that when the cod collagen peptide dosage is 2%, 4%, 6%, 8% and 10%, the overall score of the prescription is good (≥24 points), indicating that the cod collagen peptide dosage of 2-10% is qualified. It is also noted that the highest score is when the cod collagen peptide dosage is 6%.

[0083] 5. Orthogonal experimental screening of prescription composition

[0084] Based on the results of the single-factor experiment, we selected four factors with the most significant impact on the overall sensory score: synthetic squalane, sodium lauroyl glutamate, carbomer 940, and jojoba oil. We designed a four-factor, three-level orthogonal experiment to determine the optimal eye cream formula. The specific orthogonal experiment factor levels are shown in Table 11.

[0085] Table 11 Orthogonal test factor levels

[0086]

[0087] Eye cream was prepared and evaluated according to the above design table. The specific results are shown in Table 12.

[0088] Table 12 Orthogonal investigation results

[0089]

[0090] The above findings indicate that the factors influencing the sensory evaluation of eye creams are ranked as follows: sodium lauroyl glutamate > synthetic squalane = jojoba oil > carbomer 940; the optimal preparation process is 4% synthetic squalane, 5% sodium lauroyl glutamate, 1.5% carbomer 940, and 4% jojoba oil. Furthermore, the data also shows that samples prepared with all of the above combinations received good overall scores (≥24 points), indicating that products prepared within these ranges meet acceptable standards.

[0091] In summary, the prescriptions screened out for cod collagen peptide eye cream are shown in Table 13.

[0092] Table 13 Cod collagen peptide eye cream prescription

[0093]

[0094]

[0095] Note: The qualified range for prescription dosage of synthetic squalane is 1-5%; the qualified range for prescription dosage of glycerin is 3-5%; the qualified range for prescription dosage of sodium lauroyl glutamate is 1-5%; the qualified range for prescription dosage of Tween 80 is 2-4%; the qualified range for prescription dosage of jojoba oil is 1-5%; the qualified range for prescription dosage of carbomer 940 is 0.5-1.5%; the qualified range for prescription dosage of propylene glycol is 1-3%; the qualified range for prescription dosage of cod collagen peptide is 2-10%.

[0096] Example 2 Performance Evaluation of Cod Collagen Peptide Eye Cream

[0097] In each investigation under this embodiment, the cod collagen peptide eye cream samples were prepared using the prescription composition in Table 13.

[0098] 1.Physical and chemical properties

[0099] 1.1 Appearance and texture

[0100] White emulsion paste, fine and uniform texture, no granularity, light rose scent.

[0101] 1.2 pH value

[0102] Weigh 2g of eye cream (accurate to 0.1g) and add nine parts of boiled and cooled test water. Heat to 40°C, stirring continuously, and cool to room temperature before use. Before measurement, calibrate with two standard buffers. After calibration, rinse the electrode with water, then blot dry with filter paper. Carefully insert the electrode into the sample, immersing it. Wait for the pH meter reading to stabilize and record the reading.

[0103] Measurement results: 5.82, 5.76, 5.91, 5.86, 5.79, 5.80.

[0104] 1.3 Stability Test

[0105] (1) Centrifugation test: 1.5 g of sample was transferred into a 2 mL EP tube and centrifuged at 3000 rpm for 30 min. The eye cream was observed for stratification.

[0106] Result: No stratification or oil-water separation.

[0107] (2) Cold resistance test:

[0108] Set the refrigerator temperature to -8°C, transfer 1.5 g of the sample into a 2 mL EP tube, and place it in the refrigerator. Take it out after 24 hours and observe whether the eye cream has stratification.

[0109] Results: The paste remained homogeneous after returning to room temperature.

[0110] (3) Heat resistance test:

[0111] Set the constant temperature incubator to 40°C, transfer 1.5 g of the sample into a 2 mL EP tube, and place it in the constant temperature incubator. Take it out after 24 hours and observe whether the eye cream has stratification.

[0112] Results: The paste remained homogeneous after returning to room temperature.

[0113] 1.4 Light stability test

[0114] Weigh hand cream samples of the same mass and place them in groups of 3 under natural light and dark conditions for 1, 2, 3, 4, and 5 hours respectively. Observe whether the eye cream changes color, odor, delamination, or other abnormal phenomena.

[0115] Result: No discoloration, odor change, delamination or other abnormal phenomena were observed.

[0116] 1.5 Moisturizing performance test

[0117] Moisturizing effect is one of the evaluation indicators of skin care products. We accurately weighed 5g of each eye cream sample and placed it in a weighing bottle. Each group of three samples was placed in an incubator at a relative humidity of 50% and a temperature of 25°C for 12, 24, 36, and 48 hours. The water loss rate of the eye cream was calculated according to formula (1). The lower the water loss rate, the better the moisturizing effect.

[0118] In addition, according to the prescription composition in Table 13, a blank matrix eye cream sample without cod collagen peptide (ie, without adding cod collagen peptide) was prepared for a comparative study on moisturizing performance.

[0119] Water loss rate = (M0-Mn) / M0×100% (1)

[0120] Where M0 is the original mass of the sample, g; Mn is the mass of the sample after being placed for n hours, g.

[0121] Table 14 Eye cream moisturizing performance test results

[0122] sample 12h 24h 36h 48h Cod collagen peptide eye cream sample 1 2.38％ 3.12％ 5.35％ 6.42％ Cod collagen peptide eye cream sample 2 2.51％ 3.67％ 5.81％ 7.15％ Cod collagen peptide eye cream sample 3 2.69％ 3.80％ 5.99％ 7.23％ Blank matrix eye cream sample 1 3.89％ 5.33％ 7.15％ 9.17％ Blank matrix eye cream sample 2 3.76％ 5.69％ 6.90％ 9.39％ Blank matrix eye cream sample 3 3.95％ 5.55％ 7.22％ 9.03％

[0123] From the results, we can see that the 48h moisturizing rate of cod collagen peptide eye cream is significantly better than that of blank matrix eye cream.

[0124] 2. Security Testing

[0125] Thirty volunteers aged 18 to 60 with healthy skin were randomly selected to trial the eye cream. Safety testing was conducted in accordance with the human safety trial test specified in the 2022 edition of the "Technical Specifications for Safety of Cosmetics." The trial period was four weeks, and safety assessments were made according to Table 15. At the end of the trial, all participants had a skin reaction grade of 0, meaning "no reaction."

[0126] Table 15 Skin reaction grading standards for human trial tests

[0127] Skin reactions Grading No response 0 Faint erythema 1 Erythema, infiltration, papules 2 Erythema, edema, papules, blisters 3 Erythema, edema, bullae 4

[0128] 3. Antioxidant capacity determination (hydroxyl radical scavenging ability)

[0129] Prepare cod collagen peptide eye cream and blank matrix eye cream samples according to Table 13 for antioxidant capacity determination. Cod collagen peptide eye cream was prepared into sample solutions of 30, 50, 70, 90, and 120 mg / mL, and blank matrix eye cream was also prepared into sample solutions of 30, 50, 70, 90, and 120 mg / mL. The reagents were added according to the combination in Table 16. Each sample solution was reacted in a 37°C water bath for 30 minutes. The absorbance was measured at 510 nm using an enzyme marker and recorded as A1 (sample group), A2 (control group), and A3 (blank group). Calculate according to the following formula: Clearance rate (%) = (1-(A1-A3) / A2) × 100%

[0130] Table 16 Reagent addition

[0131]

[0132] See below for specific measurement results Figure 1 and Figure 2 .

[0133] The above results show that cod collagen peptide eye cream has the ability to scavenge hydroxyl free radicals and exhibits antioxidant effects.

[0134] 4. Efficacy evaluation

[0135] The efficacy of cod collagen peptide eye cream and a blank matrix eye cream was compared. Twenty healthy volunteers aged 18 to 60 were randomly divided into two groups of 10. Each group received a four-week trial of either the cod collagen peptide eye cream or the blank matrix eye cream. A smart skin tester was used to measure the moisture, oil content, elasticity, and brightness of the eye area before and after use. The results are shown in Table 17.

[0136] Table 17 Efficacy evaluation results

[0137]

[0138] Note: Compared with the pre-trial group of the same sample, *P<0.05, **P<0.01, ***P<0.001

[0139] From the results in the above table, it can be seen that cod collagen peptide eye cream can significantly improve the moisture value, elasticity value and whiteness of the test subjects' eye skin; while the blank matrix eye cream can only significantly improve the moisture value of the test subjects' eye skin, and the increase is weaker than that of cod collagen peptide eye cream.

[0140] 5. Trial effect evaluation

[0141] Twenty volunteers aged 18 to 60 with healthy skin were randomly divided into two groups of 10. One group received a cod collagen peptide eye cream, while the other received a blank matrix eye cream. Subjects were unaware of their grouping and believed they were receiving the cod collagen peptide eye cream. A questionnaire survey was conducted four weeks after the trial to assess the improvement in skin tone. Ratings were based on a scale of 1 (dissatisfied), 2 (somewhat dissatisfied), 3 (fair), 4 (satisfied), and 5 (very satisfied).

[0142] Table 18 Evaluation results

[0143]

[0144]

[0145] Note: When comparing the same evaluation index between blank matrix eye cream and cod collagen peptide eye cream, *P<0.05, **P<0.01, ***P<0.001

[0146] The results in the table above show that participants were generally satisfied with the five evaluation indicators for the cod collagen peptide eye cream, with scores ranging from 3.8 to 4.4. However, overall satisfaction with the blank matrix eye cream was lower, with scores ranging from 2.6 to 3.1. Furthermore, the blank matrix eye cream group had significantly lower scores on all indicators than the cod collagen peptide eye cream group.

[0147] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A cod collagen peptide eye cream, characterized in that: The following components are included by mass percentage: Core active ingredient: cod collagen peptide 2% to 10%; Emulsification stabilization system: sodium lauroyl glutamate 1%~5%, Tween 80 2%~4%, carbomer 940 0.5%~1.5%; Moisturizing matrix components: glycerin 3% to 5%, synthetic squalane 1% to 5%, jojoba oil 1% to 5%; Functional auxiliary ingredients: sodium alginate 0.2% to 0.5%, propylene glycol 1% to 3%, disodium EDTA 0.2% to 0.5%, vitamin C 0.2% to 0.5%, rose essence 0.5% to 1.5%; pH regulator: triethanolamine 0.3% to 0.7%; Solvent: Deionized water was made up to 100%.

2. The cod collagen peptide eye cream according to claim 1, characterized in that: The protein content of the cod collagen peptide is ≥90% (dry basis), and the hydroxyproline content is ≥8% (dry basis); the pH value of the eye cream is 5.5-6.

5.

3. A method for preparing the cod collagen peptide eye cream according to any one of claims 1 to 2, characterized in that: The following steps are involved: (a) Synthetic squalane, sodium lauroyl glutamate, Tween 80, and jojoba oil are mixed, and heated at 75-80° C. with stirring until uniform; (b) Carbomer 940 is swelled in water, and glycerin, sodium alginate, triethanolamine, propylene glycol, and a portion of water are added, and heated at 75-80° C. with stirring until uniform; (c) The phase obtained in step (b) is slowly added to the phase obtained in step (a), and the mixture is stirred at 75-80° C., and then cooled to 35-45° C.; (d) Cod collagen peptide, disodium EDTA, vitamin C, rose essence, and the remaining water are added, and the mixture is homogenized and emulsified.

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