Oral composition containing regenerative medical material and preparation method and application thereof

Abstract

The invention belongs to the field of oral care, and particularly relates to an oral composition containing regenerative medical materials as well as a preparation method and application of the oral composition. The oral composition comprises a regenerative medical material and alcohol which are ultrasonically mixed, and does not comprise other antibacterial components; wherein the regenerative medical material is prepared from the following components in parts by mass: 10 to 25 parts of P2O5, 20 to 60 parts of SiO2 and 15 to 45 parts of CaO, and the regenerative medical material is a porous material. The oral composition is used as a main active ingredient or a unique active ingredient and is applied to an oral care product, and the oral care product has at least one of the following effects of freshening breath, inhibiting dental plaque, reducing gingivitis, reducing mouth-derived halitosis and reducing non-mouth-derived halitosis.

Description

Technical Field

[0001] The present invention belongs to the field of oral care, and in particular relates to an oral composition containing a regenerative medicine material, and a preparation method and application thereof. Background Art

[0002] Currently, commonly used oral care products include toothpaste and mouthwash. Their basic functions are to clean tooth crowns, protect gums, and reduce oral bacteria concentration to prevent oral diseases. With improved living standards and a growing awareness of health, most toothpastes on the market offer breath freshening properties. However, when gum problems arise, such as redness, swelling, bleeding, and receding gums, toothpaste can only be used to remove plaque attached to the gums through clinical scraping, which has little effect. Furthermore, bad breath and oral ulcers are often associated with bacterial growth in the mouth, and food residue can exacerbate this growth. Office workers often fail to brush their teeth promptly after lunch, which can exacerbate the proliferation of oral bacteria.

[0003] Bad breath is a serious social problem, affecting up to one-third of the population. Bad breath not only causes personal discomfort but also social embarrassment for the sufferer. The gases that cause bad breath mainly include volatile sulfur compounds (VSCs), such as H2S, CH3SH, CH3SCH3, etc. Bad breath can be divided into oral and non-oral sources. Oral sources are mainly caused by volatile sulfides produced by the decomposition of proteins and glycoproteins by oral microbiota, and also include periodontal disease, acute necrotizing ulcerative gingivitis, oral ulcers, etc. Non-oral sources are generally common in gastrointestinal factors, such as gastroesophageal reflux, Helicobacter pylori, duodenal foreign body infection, etc., which produce sulfides and cause bad breath.

[0004] Therefore, it is of great significance to provide an oral care product that can reduce oral and non-oral halitosis. Summary of the Invention

[0005] The main purpose of the present invention is to provide an oral composition containing regenerative medicine materials, and its preparation method and application. The composition has relatively simple ingredients and is a composition containing regenerative medicine materials. It can freshen breath, inhibit dental plaque, reduce gingivitis, reduce oral and non-oral halitosis, and at least solve any one of the problems of halitosis, dental plaque, gingivitis, etc.

[0006] In order to achieve the above-mentioned purpose, the technical solutions adopted are as follows:

[0007] In a first aspect, an oral composition containing a regenerative medicine material, the oral composition comprising an ultrasonically mixed regenerative medicine material and alcohol, and excluding other antibacterial components;

[0008] The regenerative medicine material comprises the following components by mass: 10-25 parts of P2O5, 20-60 parts of SiO2, and 15-45 parts of CaO, and the regenerative medicine material is a porous material.

[0009] Further, the oral composition is composed of ultrasonically mixed regenerative medicine material and alcohol;

[0010] And / or, the pore volume of the regenerative medicine material is 0.1-0.5 cc / g.

[0011] Furthermore, the alcohol includes one or more of glycerol, propylene glycol, ethanol, ethylene glycol, hexylene glycol, inositol, n-butanol, ethanol and methanol.

[0012] Furthermore, the mass ratio of the regenerative medicine material to the alcohol is 1:1-10.

[0013] Furthermore, the mass ratio of the regenerative medicine material to the alcohol is 1:1-5.

[0014] Furthermore, the ultrasonic mixing has an ultrasonic frequency of 10 to 50 kHz.

[0015] Furthermore, the ultrasonic mixing time is 2 to 20 minutes.

[0016] In a second aspect, a method for preparing an oral composition as described in any one of the first aspects is provided, wherein the regenerative medicine material is first dissolved in alcohol, and then ultrasonically treated to obtain the oral composition after uniform dispersion.

[0017] In a third aspect, a use of an oral composition as described in any one of the first aspects is provided, wherein the oral composition is used as the main active ingredient or the only active ingredient in an oral care product, wherein the oral care product has at least one of the following effects: freshening breath, inhibiting dental plaque, reducing gingivitis, reducing oral halitosis, and reducing non-oral halitosis.

[0018] Furthermore, the oral care product may be in the form of a cream, a solution, a paste, an ointment, a gel or a lotion;

[0019] And / or, the ingredients of the oral care product include a matrix, excipients, carriers, and additives according to the needs of toothpaste, mouthwash or spray.

[0020] Beneficial effects of the present invention:

[0021] 1. The oral composition provided by the present invention is a solid-liquid composition containing a regenerative medicine material, which can freshen breath, inhibit dental plaque, reduce gingivitis, and reduce oral and non-oral halitosis. By compounding the regenerative medicine material with alcohol, some of the alcohol enters the pores of the regenerative medicine material and can be gradually released, avoiding the situation where all the alcohol is exposed to the outside and a large amount is applied at one time, thereby greatly reducing the side effects of large amounts of alcohol on the oral mucosa, such as pain, burning, and irritation. In addition, by ultrasonically mixing the regenerative medicine material and the alcohol, the alcohol more fully enters the porous structure of the regenerative medicine material, and the two are in full contact, showing a synergistic effect of the regenerative medicine material and the alcohol, and the efficacy of reducing oral and non-oral halitosis is significantly enhanced. Simple physical mixing cannot allow the alcohol to fully enter the porous structure of the material.

[0022] In addition, the oral composition provided by the present invention has a simple formula and does not contain additional bactericidal ingredients. Through the synergistic effect of regenerative medicine materials and alcohol, the damage of bactericidal ingredients to oral flora is reduced, thereby promoting oral health.

[0023] 2. The preparation method of the oral composition provided by the present invention is simple and easy. Ultrasound can enable alcohol to more fully penetrate the porous structure of the regenerative medicine material, so that the alcohol and the regenerative medicine material are in full contact and exert their efficacy. The composition obtained by this preparation method shows a synergistic effect between the regenerative medicine material and the alcohol, and its efficacy in reducing oral and non-oral halitosis is significantly enhanced. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical solutions and advantages of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Those skilled in the art should understand that the embodiments are merely to help understand the present invention and should not be regarded as specific limitations of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative work are within the scope of protection of the present invention. If specific techniques or conditions are not specified in the embodiments, they shall be carried out according to the techniques or conditions described in the literature in this area, or according to the product instructions. If the manufacturers of the reagents or instruments used are not specified, they shall be conventional products that can be purchased through regular channels.

[0025] The endpoints of the ranges and any values ​​disclosed herein are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoints of each range, the endpoints of each range and individual point values, and the individual point values ​​can be combined with each other to form one or more new numerical ranges, and these numerical ranges should be considered to be specifically disclosed in the present invention.

[0026] According to a first aspect of the present invention, there is provided an oral composition containing a regenerative medicine material, comprising an ultrasonically mixed regenerative medicine material and alcohol, and excluding other antibacterial components;

[0027] Wherein, the regenerative medicine material comprises the following components in parts by mass: 10-25 parts of P2O5 (such as 11 parts, 13 parts, 15 parts, 17 parts, 19 parts, 21 parts, 23 parts, etc.), 20-60 parts of SiO2 (such as 21 parts, 23 parts, 25 parts, 27 parts, 29 parts, 31 parts, 33 parts, 35 parts, 37 parts, 39 parts, 41 parts, 43 parts, 45 parts, 47 parts, 49 parts, 51 parts, 53 parts, 55 parts, 57 parts, 59 parts, etc.), and 15-45 parts of CaO (such as 16 parts, 18 parts, 20 parts, 22 parts, 24 parts, 26 parts, 28 parts, 30 parts, 32 parts, 34 parts, 36 parts, 38 parts, 40 parts, 42 parts, 44 parts, etc.), and the regenerative medicine material is a porous material.

[0028] The oral composition provided by the present invention is a solid-liquid composition containing a regenerative medicine material. The composition can freshen breath, inhibit dental plaque, reduce gingivitis, and reduce oral and non-oral halitosis. By compounding the regenerative medicine material with alcohol, part of the alcohol enters the pores of the regenerative medicine material and can be gradually released, avoiding complete exposure of the alcohol and a large amount of alcohol applied at one time, thereby greatly reducing side effects such as pain, burning, and irritation on the oral mucosa caused by a large amount of alcohol. Furthermore, by ultrasonically mixing the regenerative medicine material and the alcohol, the alcohol more fully enters the porous structure of the regenerative medicine material, and the two are in full contact, demonstrating a synergistic effect of the regenerative medicine material and the alcohol, and significantly enhancing the efficacy of reducing oral and non-oral halitosis. Simple physical mixing cannot fully allow the alcohol to enter the porous structure of the material.

[0029] In addition, the composition provided by the present invention has a simple formula and does not contain additional bactericidal ingredients. Through the synergistic effect of regenerative medicine materials and alcohol, the damage of bactericidal ingredients to oral flora is reduced, thereby promoting oral health.

[0030] As an optional embodiment of the oral composition of the present invention, the pore volume of the regenerative medicine material is 0.1-0.5cc / g (such as 0.15cc / g, 0.2cc / g, 0.25cc / g, 0.3cc / g, 0.35cc / g, 0.4cc / g, 0.45cc / g, etc.).

[0031] As an optional embodiment of the oral composition of the present invention, the oral composition consists of ultrasonically mixed regenerative medicine material and alcohol.

[0032] As an optional embodiment of the oral composition of the present invention, the regenerative medicine material includes the following components in parts by mass: 10-25 parts of P2O5 (such as 11 parts, 13 parts, 15 parts, 17 parts, 19 parts, 21 parts, 23 parts, etc.), 20-60 parts of SiO2 (such as 21 parts, 23 parts, 25 parts, 27 parts, 29 parts, 31 parts, 33 parts, 35 parts, 37 parts, 39 parts, 41 parts, 43 parts, 45 parts, 47 parts, 49 parts, 51 parts, 53 parts, 55 parts, 57 parts, 59 parts, etc.), and 15-45 parts of CaO (such as 16 parts, 18 parts, 20 parts, 22 parts, 24 parts, 26 parts, 28 parts, 30 parts, 32 parts, 34 parts, 36 parts, 38 parts, 40 parts, 42 parts, 44 parts, etc.), totaling 100 parts.

[0033] As an optional embodiment of the oral composition of the present invention, the regenerative medicine material is Regesi regenerative silicon.

[0034] As an optional embodiment of the oral composition of the present invention, the alcohol includes one or more of glycerol, propylene glycol, ethanol, ethylene glycol, hexylene glycol, inositol, n-butanol, ethanol, methanol, etc.

[0035] As an optional embodiment of the oral composition of the present invention, the mass ratio of the regenerative medicine material to the alcohol is 1:1-10 (e.g., 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, etc.), preferably 1:1-5 (e.g., 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, etc.). This ensures that sufficient alcohol is present within the pores of the regenerative medicine material while preventing irritation and other side effects caused by excessive alcohol content. A low alcohol content, particularly an uneven or insufficient distribution of alcohol content within the pores, can result in slow or insufficient alcohol release, affecting the synergistic effect of the regenerative medicine material and the alcohol.

[0036] According to the second aspect of the present invention, a method for preparing the oral composition is further provided, wherein the regenerative medicine material is first dissolved in alcohol, and then ultrasonically treated to uniformly disperse the material to obtain the oral composition.

[0037] In the above-mentioned oral composition or preparation method thereof, as a preferred embodiment, the ultrasound frequency is 10 to 50 kHz (e.g., 15 kHz, 20 kHz, 25 kHz, 30 kHz, 35 kHz, 40 kHz, 45 kHz, etc.), more preferably 20 kHz. Ultrasonic frequencies below 10 kHz are not conducive to uniform dispersion of the regenerative medicine material in the alcohol base. Ultrasonic frequencies above 50 kHz destroy the porous structure of the regenerative medicine material, resulting in fewer pores.

[0038] In the above oral composition or its preparation method, as a preferred embodiment, the ultrasonic time is 2 to 20 minutes (for example: 3 minutes, 5 minutes, 7 minutes, 10 minutes, 12 minutes, 15 minutes, 16 minutes, 18 minutes, 19 minutes, etc.), and more preferably 10 minutes.

[0039] According to a third aspect of the present invention, there is also provided an application of the oral composition, including application of the composition in oral care articles / products having any of the functions of freshening breath, inhibiting dental plaque, reducing gingivitis, and reducing oral and non-oral halitosis.

[0040] The present invention also provides an oral care material / product, which contains the oral composition as a main active ingredient or the only active ingredient.

[0041] For the composition and preparation of the above-mentioned oral care products, reference may be made to the prior art.

[0042] The oral care product can be in the form of a cream, lotion, paste, ointment, gel, or lotion, etc. The base, excipients, carriers, additives, etc. known in the art can be mixed according to conventional methods and prepared into toothpaste, mouthwash, spray, etc.

[0043] The Regesi recycled silicon used in the following examples and comparative examples is a commercially available regenerative medicine material product produced by Beijing Xingfu Yisheng High-tech Co., Ltd. The material is Regesi recycled silicon composed of SiO2, CaO, and P2O5, and is in the form of solid powder with a large specific surface area (360m 2 / g or more), and has a stable pH after contact with body fluids.

[0044] In the following examples and comparative examples, the mass parts are based on the same standard. For example, the mass ratio of Regesi regenerated silicon to glycerol in Example 1 is 1:5, and the mass ratio of Regesi regenerated silicon to glycerol in Example 2 is 1:1. 1 mass part of Regesi regenerated silicon in Example 1 is the same as 1 mass part of Regesi regenerated silicon in Comparative Examples 1 and 2. Other similarities will not be repeated here.

[0045] Example 1

[0046] This embodiment provides an oral composition and a preparation method thereof, and the specific steps are as follows:

[0047] 1 part by mass of Regesi regenerated silicon (pore volume of 0.3 cc / g) was added to 5 parts by mass of glycerol (propylene glycol), and ultrasonically treated (20 kHz, 10 min) to obtain a regenerative medicine material composition.

[0048] Example 2

[0049] This embodiment provides an oral composition and a preparation method thereof, and the specific steps are as follows:

[0050] 1 part by mass of Regesi regenerated silicon (pore volume of 0.3 cc / g) was added to 1 part by mass of glycerol and ultrasonically treated (20 kHz, 10 min) to obtain a regenerative medicine material composition.

[0051] Example 3

[0052] This embodiment provides an oral composition and a preparation method thereof, and the specific steps are as follows:

[0053] 1 part by mass of Regesi regenerated silicon (pore volume of 0.3 cc / g) was added to 10 parts by mass of glycerol and ultrasonically treated (20 kHz, 10 min) to obtain a regenerative medicine material composition.

[0054] Example 4

[0055] This embodiment provides an oral composition and a preparation method thereof, and the specific steps are as follows:

[0056] 1 part by mass of Regesi regenerated silicon (pore volume of 0.3 cc / g) was added to 5 parts by mass of glycerol and ultrasonically treated (10 kHz, 10 min) to obtain a regenerative medicine material composition.

[0057] Example 5

[0058] This embodiment provides an oral composition and a preparation method thereof, and the specific steps are as follows:

[0059] 1 part by mass of Regesi regenerated silicon (pore volume of 0.3 cc / g) was added to 5 parts by mass of glycerol and ultrasonically treated (50 kHz, 10 min) to obtain a regenerative medicine material composition.

[0060] Example 6

[0061] This embodiment provides an oral composition and a preparation method thereof, and the specific steps are as follows:

[0062] 1 part by mass of Regesi regenerated silicon (pore volume of 0.1 cc / g) was added to 5 parts by mass of glycerol (propylene glycol), and ultrasonically treated (20 kHz, 10 min) to obtain a regenerative medicine material composition.

[0063] Example 7

[0064] This embodiment provides an oral composition and a preparation method thereof, and the specific steps are as follows:

[0065] 1 part by mass of Regesi regenerated silicon (pore volume of 0.5 cc / g) was added to 5 parts by mass of glycerol (propylene glycol), and ultrasonically treated (20 kHz, 10 min) to obtain a regenerative medicine material composition.

[0066] Example 8

[0067] This embodiment provides an oral composition and a preparation method thereof, and the specific steps are as follows:

[0068] 1 part by mass of Regesi regenerated silicon (pore volume of 0.3 cc / g) was added to 5 parts by mass of propylene glycol, and ultrasonic treatment was performed (20 kHz, 10 min) to obtain a regenerative medicine material composition.

[0069] Example 9

[0070] This embodiment provides an oral composition and a preparation method thereof, and the specific steps are as follows:

[0071] 1 part by mass of Regesi regenerated silicon (pore volume of 0.3 cc / g) was added to 5 parts by mass of butanediol, and ultrasonic treatment (20 kHz, 10 min) was performed to obtain a regenerative medicine material composition.

[0072] Example 10

[0073] This embodiment provides a toothpaste having the following composition (by weight): 30% of the oral composition of Example 1 and 70% of the toothpaste base.

[0074] The composition of the toothpaste matrix is ​​as follows (mass percentage):

[0075] Polyvinylpyrrolidone 0.2%, sodium lauroyl sarcosinate 2%, hydrated silica 15%, flavor 0.2%, trichlorogalactose 2%; polyethylene glycol-88 0.6%.

[0076] Example 11

[0077] This embodiment provides a toothpaste having the following composition (by weight): 10% of the oral composition of Example 2 and 90% of a toothpaste base.

[0078] The toothpaste base formula is the same as that in Example 10.

[0079] Example 12

[0080] This embodiment provides a toothpaste having the following composition (by weight): 55% of the oral composition of Example 3 and 45% of a toothpaste base.

[0081] The toothpaste base formula is the same as that in Example 10.

[0082] Example 13

[0083] This embodiment provides a toothpaste having the following composition (by weight): 30% of the oral composition of Example 4 and 70% of the toothpaste base.

[0084] The toothpaste base formula is the same as that in Example 10.

[0085] Example 14

[0086] This embodiment provides a toothpaste having the following composition (by weight): 30% of the oral composition of Example 5 and 70% of the toothpaste base.

[0087] The toothpaste base formula is the same as that in Example 10.

[0088] Example 15

[0089] This embodiment provides a toothpaste having the following composition (by weight): 30% of the oral composition of Example 6 and 70% of the toothpaste base.

[0090] The toothpaste base formula is the same as that in Example 10.

[0091] Example 16

[0092] This embodiment provides a toothpaste having the following composition (by weight): 30% of the oral composition of Example 7 and 70% of the toothpaste base.

[0093] The toothpaste base formula is the same as that in Example 10.

[0094] Example 17

[0095] This embodiment provides a toothpaste having the following composition (by weight): 30% of the oral composition of Example 8 and 70% of the toothpaste base.

[0096] The toothpaste base formula is the same as that in Example 10.

[0097] Example 18

[0098] This embodiment provides a toothpaste having the following composition (by weight): 30% of the oral composition of Example 9 and 70% of the toothpaste base.

[0099] The toothpaste base formula is the same as that in Example 8.

[0100] Comparative Example 1

[0101] 1 part by mass of Regesi regenerated silicon (pore volume 0.3 cc / g) was added to 5 parts by mass of glycerol and stirred until uniformly dispersed to obtain a regenerative medicine material composition.

[0102] Comparative Example 2

[0103] Take 1 part by mass of Regesi regenerated silicon (pore volume 0.3 cc / g), 20 kHz ultrasonic treatment for 10 min.

[0104] Comparative Example 3

[0105] Take 1 part by mass of glycerol, 20 kHz ultrasonic treatment for 10 min.

[0106] Comparative Example 4

[0107] This comparative example provides a toothpaste, the composition of which is as follows (mass percentage): the product of Comparative Example 1 30%, toothpaste base 70%.

[0108] The toothpaste base formula is the same as Example 10.

[0109] Comparative Example 5

[0110] This comparative example provides a toothpaste, the composition of which is as follows (mass percentage): the product of Comparative Example 2 5%, toothpaste base 95%.

[0111] The toothpaste base formula is the same as Example 10.

[0112] Comparative Example 6

[0113] This comparative example provides a toothpaste, the composition of which is as follows (mass percentage): the product of Comparative Example 3 25%, toothpaste base 75%.

[0114] The toothpaste base formula is the same as Example 10.

[0115] Comparative Example 7

[0116] This comparative example provides a toothpaste base.

[0117] The toothpaste base formula is the same as Example 10.

[0118] The pore volume of Regesi used in the above example and comparative example compositions and the mass percentage of the toothpaste components formed are as follows:

[0119]

[0120]

[0121] Experimental Example 1 Toothpaste Inhibition Zone Test on P. g

[0122] Experimental principle: according to the 2002 version of the Ministry of Health "Disinfection Technical Specification" 2.1.8.2 inhibition zone test, the negative control product is the base toothpaste in Comparative Example 7.

[0123] Prepare the bacterial suspension: Prepare three turbidity vials and add 4ml, 8ml, and 10ml of phosphate buffered saline (PBS) (0.03 mol / L, pH 7.2), respectively. Scrape half of the P. gingivalis culture from the plate into a 4ml PBS turbidity tube. Shake well, then add liquid paraffin and seal. This solution serves as the stock solution. Use a pipette to transfer 100µl of the stock solution to 8ml of PBS and shake well. Adjust the concentration of the bacterial suspension on the turbidity meter. First, calibrate the turbidity vial with 10ml of PBS, then measure the turbidity until the turbidity reaches 0.5 MCF (1.5 × 108 cfu / ml).

[0124] Bacterial liquid coating: mark the blood agar plate, use a pipette to draw 100ul of 0.5MCF bacterial suspension onto the blood agar plate, spread it evenly with a sterile cotton swab, and dry it at room temperature for 5 minutes before use.

[0125] Preparation of test toothpaste plates: Triangular holes approximately 6 mm in diameter were cut out on blood agar plates. The toothpastes from Examples 10-18 and Comparative Examples 4-7 were uniformly squeezed into sterile syringes. 50 mg of toothpaste was injected into the holes through a needle. Three plates were plated for each sample. The plates were placed in anaerobic culture bags, containing a gas production bag and an oxygen indicator. The plates were then placed in a 37°C incubator. After 48 hours, the diameter of the inhibition ring was measured with a vernier caliper. The average value of the three plates was taken. A larger inhibition ring diameter indicated a stronger antibacterial effect. Ring diameters greater than 7 mm were considered to have an antibacterial effect; those less than or equal to 7 mm were considered to have no antibacterial effect. The antibacterial effect against Porphyromonas gingivalis is shown in Table 1.

[0126] Table 1 Antibacterial effect of toothpaste on Porphyromonas gingivalis Pg

[0127] Group Inhibition ring (mm) Example 10 20.6 Example 11 16.2 Example 12 15.7 Example 13 10.2 Example 14 10.3 Example 15 18.6 Example 16 17.9 Example 17 18.2 Example 18 18.7 Comparative Example 4 9.2 Comparative Example 5 8.5 Comparative Example 6 3.6 Comparative Example 7 2.7

[0128] As shown in Table 1, Examples 10-18 all have inhibitory effects on Porphyromonas gingivalis, among which Example 10 has the best effect. At the same time, the inhibition rates of Examples 10-18 are better than those of Comparative Examples 4-7, indicating that the oral composition prepared by the present invention has excellent inhibitory effects on Porphyromonas gingivalis.

[0129] Experimental Example 2 Clinical Trial on the Efficacy of Toothpaste in Alleviating Bad Breath

[0130] Experimental Method: 130 male subjects with oral halitosis, frequent smoking, and alcohol consumption were randomly divided into 13 groups, each with 10 subjects. The subjects were tested using toothpastes from Examples 10-18 and Comparative Examples 4-7. 2g of each toothpaste was squeezed onto a standard toothbrush 2cm apart and brushed for 3 minutes. The subjects' oral VSCs concentrations were measured before rinsing and brushing; 5 minutes after brushing; 60 minutes after brushing; and 120 minutes after brushing.

[0131] The test instrument used was a Halimeter breath meter. It uses electrochemical principles to measure the concentration of volatile sulfur compounds (VSCs) in oral air as an indicator of the patient's breath quality. Before use, the meter was zeroed with ambient air. During measurement, the subject breathed through their nose with their mouth slightly open. The mouth of the gas collection tube was placed 0.5 cm above the junction of the middle and posterior thirds of the tongue dorsum. The value on the display was read. Each test was repeated three times, and the results were averaged. The results of the toothpaste's effectiveness in reducing bad breath are shown in Table 2.

[0132] Table 2 Test results of toothpaste's effect in reducing bad breath

[0133]

[0134] As shown in Table 2, Examples 10-18 can all reduce the concentration of VSCs in the oral cavity, among which Example 6 has the best effect. At the same time, the concentration of VSCs in Examples 10-18 is lower than that in Comparative Examples 4-7, indicating that the composition prepared by the present invention has an excellent effect of reducing bad breath.

[0135] Experimental Example 3 Anti-inflammatory Test

[0136] Anti-inflammatory tests were performed on the toothpastes of Examples 10-18 and Comparative Examples 4-7.

[0137] Test reagents and methods

[0138] Reagent: Inflammatory agent, i.e. 100% xylene.

[0139] Test method: The toothpastes of Examples 10-18 and Comparative Examples 4-7 were mixed with an inflammatory agent (100% xylene) at a volume ratio of 1:1 as the inflammatory agent containing the sample, and compared with the pure inflammatory agent for a comparative test. 140 mice were selected and randomly divided into 14 groups, 10 mice in each group, and divided into a blank control group and a sample group. The inflammatory agent (blank control group, 0.02 mL / mouse) or the inflammatory agent containing the sample (test group, 0.04 mL / mouse) was applied to the front and back surfaces of the right ear of the test mice. The left ear was not treated in any way. After 1 hour, the animals were anesthetized and killed, both ears were cut off, and round ear pieces were punched out from the same part with a 9 mm diameter punch and weighed. The degree of swelling was calculated by subtracting the weight of the left ear piece from the weight of the right ear piece of each mouse. The swelling degrees of the sample group and the blank control group were statistically processed to calculate the swelling inhibition rate (%), where swelling inhibition rate (%) = (weight of the inflamed ear - weight of the non-inflamed ear) / weight of the non-inflamed ear * 100%.

[0140] The experimental results are shown in Table 3.

[0141] Table 3 Effects of samples on ear swelling in mice

[0142]

[0143] As shown in Table 3, Examples 10-18 can effectively reduce the swelling of the mouse ears and have anti-inflammatory effects, among which Example 10 has the best effect. At the same time, the swelling inhibition rates of Examples 10-18 are better than those of Comparative Examples 4-7, indicating that the composition prepared by the present invention has excellent anti-inflammatory effects.

[0144] Although the present invention has been described in detail above using general descriptions and specific embodiments, it will be apparent to those skilled in the art that modifications and improvements may be made based on the present invention. Therefore, such modifications and improvements, which do not depart from the spirit of the present invention, are intended to be within the scope of protection claimed herein.

Claims

1. An oral composition containing a regenerative medicine material, characterized in that: The oral composition includes an ultrasonically mixed regenerative medicine material and alcohol, and does not include other antimicrobial ingredients; The regenerative medicine material comprises the following components by mass: 10-25 parts of P2O5, 20-60 parts of SiO2, and 15-45 parts of CaO, and the regenerative medicine material is a porous material.

2. The oral composition according to claim 1, wherein The oral composition is composed of an ultrasonically mixed regenerative medicine material and alcohol; And / or, the pore volume of the regenerative medicine material is 0.1-0.5 cc / g.

3. The oral composition according to claim 1, wherein The alcohol includes one or more of glycerol, propylene glycol, ethanol, ethylene glycol, hexylene glycol, inositol, n-butanol, ethanol and methanol.

4. The oral composition according to claim 1, wherein The mass ratio of the regenerative medicine material to the alcohol is 1:1-10.

5. The oral composition according to claim 4, wherein The mass ratio of the regenerative medicine material to the alcohol is 1:1-5.

6. The oral composition according to claim 1, wherein The ultrasonic mixing has an ultrasonic frequency of 10 to 50 kHz.

7. The oral composition according to claim 1, wherein The ultrasonic mixing time is 2 to 20 minutes.

8. A method for preparing the oral composition according to any one of claims 1 to 7, characterized in that: The regenerative medicine material is firstly dissolved in alcohol, and then ultrasonically treated to obtain an oral composition after being evenly dispersed.

9. A use of the oral composition according to any one of claims 1 to 7, characterized in that: The oral composition is used as the main active ingredient or the only active ingredient in an oral care product, and the oral care product has at least one of the following functions: freshening breath, inhibiting dental plaque, reducing gingivitis, reducing oral halitosis, and reducing non-oral halitosis.

10. The use according to claim 9, characterized in that The dosage form of the oral care product includes any one of a cream, a liquid, a paste, an ointment, a gel or a lotion; And / or, the ingredients of the oral care product include a matrix, excipients, carriers, and additives according to the needs of toothpaste, mouthwash or spray.

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