Method for high-throughput preparation of heart organoid

By using growth factor-free culture medium and gradient addition of small molecule compounds during the preparation of cardiac organoids, the problems of high cost and cell loss have been solved, enabling low-cost, high-throughput preparation of cardiac organoids containing multiple cell types, which is suitable for drug toxicity testing.

CN120843409APending Publication Date: 2025-10-28INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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Patent Information

Application Number
CN202510973280.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-07-15
Publication Date
2025-10-28

AI Technical Summary

Technical Problem

Existing technologies are costly and carry the risk of cell loss during the preparation of heart organoids, making it difficult to achieve high-throughput preparation.

Method used

Using a culture method that does not require the addition of additional growth factors, iPSCs were induced to form cardiac organoids by gradient addition of B27 and specific small molecule compounds CHIR99021 and IWR1 in RPMI 1640 basal medium, which reduced the complexity of operation and the risk of cell loss.

Benefits of technology

This technology enables low-cost, high-throughput preparation of cardiac organoids. The resulting organs contain multiple cell types, can respond to drug-induced changes in contraction frequency, and are suitable for drug toxicity testing.

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Abstract

The invention discloses a method for high-throughput preparation of heart organoid. Relates to the technical field of biology. Comprising the following steps: culturing iPSC; performing iPSC digestion, inoculation and culture before induction; performing induction; and continuously culturing. The method can be used for simply preparing a large number of heart organs. The preparation method is simple and convenient, has low requirements on materials and equipment, and needs few reagents. And a large number of organoid can be obtained in the same batch. The organoid is composed of various cell types such as myocardial cells, fibroblasts, vascular endothelial cells and the like. The contraction of the cardiac organoids is induced in response to a drug.
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