Liquid hybridization probe pool, kit, and combined detection method for multiple pathogens and drug resistance genes

By designing unequal-length probe pools and a DNA/RNA co-construction library strategy using liquid-phase hybridization capture technology, the problems of low detection efficiency of respiratory pathogens and monitoring of viral mutations were solved, enabling efficient joint detection and tracing of multiple pathogens and drug-resistant genes.

CN120843744BActive Publication Date: 2026-01-30BEIJING YOUAN HOSPITAL CAPITAL MEDICAL UNIV
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Patent Information

Application Number
CN202511351212.9
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-09-22
Publication Date
2026-01-30
Estimated Expiration
2045-09-22

AI Technical Summary

Technical Problem

Existing technologies are inefficient and have limited coverage in detecting respiratory pathogens. They cannot monitor viral mutations in real time, and drug resistance gene detection is time-consuming, making it difficult to meet the rapid diagnostic needs of acute and severe infections.

Method used

A probe pool of unequal lengths was designed using liquid-phase hybridization capture technology. The probes contain target-specific sequences and conjugated sequences. Through complementary pairing of the 5' and 3' end conjugated sequences, combined with a DNA/RNA co-construction library strategy, efficient joint detection of multiple pathogens and drug resistance genes can be achieved.

Benefits of technology

It enables efficient detection of multiple subtypes of pathogens, tracks pathogen variation patterns, shortens detection time, and improves detection sensitivity and specificity. It is applicable to various clinical sample types and supports pathogen tracing and drug resistance monitoring.

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Abstract

This invention discloses a liquid-phase hybridization probe pool for the joint detection of multiple pathogens and drug resistance genes, designed according to the target regions of the target pathogens and drug resistance genes. The probes are of varying lengths from 30 to 100 nt, including target-specific sequences complementary to the target region and conjugate sequences complementary to adjacent probes. The 5' and 3' ends of the probes are modified with conjugate sequences A and B, respectively, enabling specific binding between adjacent probes through end-pairing. Conjugate sequence A is shown in SEQ ID No. 601, and conjugate sequence B is shown in SEQ ID No. 602. This invention utilizes library construction and specific probe pool hybridization to shorten the capture time to 30 min, significantly outperforming traditional methods. This optimization not only reduces operation time and complexity and minimizes human error but also maintains high sensitivity and specificity, enabling efficient capture of target nucleic acids in complex samples, providing high-quality templates for subsequent sequencing, and supporting the joint detection of multiple respiratory pathogens and drug resistance genes.
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