Method for biosynthesizing ginsenoside by using tobacco BY-2 cells as chassis

By reconstructing the ginsenoside synthesis pathway in tobacco BY-2 cells, constructing recombinant expression vectors, and screening positive cell lines, the problems of low synthesis efficiency and poor stability of rare ginsenosides were solved, achieving efficient and stable heterologous synthesis of rare ginsenosides, which is suitable for industrial production.

CN120924600AActive Publication Date: 2025-11-11ZHEJIANG FINDYOU BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202511454424.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-10-13
Publication Date
2025-11-11
Estimated Expiration
2045-10-13

AI Technical Summary

Technical Problem

Rare ginsenosides are present in extremely low amounts in natural ginseng plants. Existing synthesis methods are inefficient and unstable, making it difficult to meet the needs of industrial production.

Method used

The ginsenoside synthesis pathway was reconstructed in tobacco BY-2 cells through genetic engineering. The recombinant expression vector pYLTAC380GW-tHMGR-SE-SS-PgPPDs-PgDDS-Pn1-31-Pn3-31-FPS-IDI was constructed and transformed into tobacco BY-2 cells using Agrobacterium-mediated transformation. Positive cell lines were then screened for ginsenoside synthesis.

Benefits of technology

This study achieved efficient and stable heterologous synthesis of rare ginsenosides Rh2 and Rg3, freeing us from dependence on natural plants and providing a reproducible technical solution for industrial production.

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Abstract

The invention discloses a method for biosynthesizing ginsenoside by taking tobacco BY-2 cells as a chassis, and belongs to the technical field of bioengineering. The method comprises the following steps that firstly, a recombinant expression vector containing a ginsenoside synthesis pathway key gene set is constructed, and the gene set comprises genes such as tHMGR, SE and SS from ginseng and Pn1-31 and Pn3-31 genes from panax notoginseng; secondly, introducing the recombinant vector into tobacco BY-2 cells, and screening through a solid culture medium to obtain a pure positive cell line; and finally, culturing the positive cell line in a liquid or solid culture medium to obtain ginsenoside Rh2 and / or Rg3. By utilizing the advantages of fast growth and stable culture of the tobacco BY-2 cells, a rare ginsenoside synthesis pathway is successfully reconstructed, efficient heterologous synthesis of the rare ginsenoside is realized, and a repeatable technical scheme is provided for industrial production of the rare ginsenoside.
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