Production technology flow of largemouth bass with delayed gonad development

By silencing key genes related to the development of male and female gonads in largemouth bass using antisense RNA technology, gonadal development is delayed, solving the problems of slow growth rate and poor meat quality in largemouth bass, improving growth rate and meat flavor, and promoting the sustainable development of the industry.

CN121065268AActive Publication Date: 2025-12-05FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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Patent Information

Application Number
CN202511029782.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-07-25
Publication Date
2025-12-05
Estimated Expiration
2045-07-25

AI Technical Summary

Technical Problem

Current technology lacks methods to construct largemouth bass with delayed gonadal development, fast growth rate, and delicious meat. As a result, female largemouth bass grow slower, have poorer body shape, are more prone to roe loss, and have a high mortality rate, which affects the development of the industry.

Method used

By silencing key genes dmrt1, Tekt1, btg4, and hsd17b12a for gonad development in largemouth bass using antisense RNA technology, their expression levels were reduced, resulting in a new germplasm with delayed gonad development.

Benefits of technology

It significantly improves growth rate, reduces gonad index, and enhances meat flavor, solving the problems of slow growth rate and poor meat quality in female largemouth bass, and enhancing the sustainable development capacity of the industry.

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Abstract

The invention discloses a production technology flow of largemouth bass with delayed gonad development, and belongs to the technical field of aquatic animal biological breeding. The method comprises the following steps: breeding parents, designing an antisense RNA sequence, amplifying, preparing a transfection reagent, carrying out artificial insemination on the micropterus salmoides, and introducing the antisense RNA sequence of a sex development key gene, thereby creating a new germplasm of the micropterus salmoides with remarkably improved growth speed, remarkably reduced gonad index and better muscle quality and flavor; the technical support is provided for promoting the high-quality sustainable development of the largemouth bass industry.
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