Multiple PCR-capillary electrophoresis detection system for identifying 13 species and special primer combination thereof
By designing specific primer combinations and capillary electrophoresis technology, the problems of low resolution and insufficient sensitivity of multiplex PCR technology in species identification were solved, achieving high-sensitivity identification of 13 species, which is suitable for forensic evidence and food safety monitoring.
Patent Information
- Application Number
- CN202511409088.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-29
- Publication Date
- 2025-12-05
AI Technical Summary
Existing multiplex PCR technology suffers from low resolution and insufficient sensitivity in species identification, making it difficult to meet the regulatory and case-handling needs of public security organs. Furthermore, existing methods cannot detect multiple species simultaneously, failing to meet practical application requirements.
A primer combination consisting of 13 pairs of specific primers was designed to achieve efficient identification of 13 species, including chicken, duck, sheep, pig, cattle, mouse, rat, cat, dog, donkey, horse, human and Chinese hamster, through multiplex PCR combined with capillary electrophoresis. Fluorescent labeling was used to improve detection accuracy.
It achieves highly sensitive identification of 13 species, can detect with a template amount of 0.05 ng, and has a higher sensitivity than traditional agarose gel electrophoresis. It can accurately distinguish multiple DNA fragments of similar length, and is suitable for forensic evidence analysis and food safety monitoring, with good application value.