Alpha-canthine specific nucleic acid aptamer and test paper detection method thereof

By using SELEX technology to screen CHA-specific nucleic acid aptamers and gold-labeled probes, and combining them with lateral flow chromatography test strips, the problems of long testing time and high cost of existing CHA tests have been solved, enabling rapid, accurate, and low-cost CHA testing under non-laboratory conditions.

CN121160701APending Publication Date: 2025-12-19OIL CROPS RES INST CHINESE ACAD OF AGRI SCI
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Patent Information

Application Number
CN202511327741.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-09-17
Publication Date
2025-12-19

AI Technical Summary

Technical Problem

Existing CHA detection technologies are time-consuming, costly, cumbersome to operate, and difficult to achieve real-time detection. They are also susceptible to environmental influences and cannot quickly and accurately detect the content of α-carboxine in potatoes in non-laboratory settings.

Method used

High-affinity CHA-specific nucleic acid aptamers were obtained by screening using SELEX technology. Combined with gold-labeled probes and lateral flow chromatography test strips, rapid and visual detection was achieved through the competitive binding principle.

Benefits of technology

It enables rapid and accurate detection of CHA in potatoes under non-laboratory conditions, with improved sensitivity, suitable for field applications, and can complete the detection within 15 minutes. The results are highly consistent with LC-MS/MS, with strong specificity, adaptability to complex matrices, high stability, and low cost.

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Abstract

The invention provides a specific nucleic acid aptamer of alpha-camarine and a test paper detection method of the specific nucleic acid aptamer. The nucleic acid aptamer has a nucleic acid sequence as shown in SEQ ID NO. 1 or SEQ ID NO. 2. The invention also provides a lateral flow chromatography detection test strip for alpha-calasodine. The lateral flow chromatography detection test strip comprises a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, the conjugate pad is sprayed with a gold-labeled probe containing the aptamer and gold nanoparticles; the nitrocellulose membrane is provided with a detection line and a quality control line, the detection line is fixedly provided with a first capture molecule, the first capture molecule is streptavidin, the quality control line is fixedly provided with a second capture molecule, and the second capture molecule is a conjugate of a streptavidin-biotinylated aptamer. According to the nucleic acid aptamer, the gold-labeled probe and the test strip disclosed by the invention, the detection efficiency of the alpha-camarin detection method is greatly improved, and the on-site instant demand is met.
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