Accurate evaluation method for tomato rootstock resistance to elephantopus scaber root-knot nematode

By combining growth indicators, disease resistance indicators, and soil microbial diversity indices, the ability of tomato rootstocks to resist weevils and root-knot nematodes was accurately evaluated, solving the problem of inaccurate evaluation in existing technologies and realizing the precise screening and comprehensive evaluation of resistant rootstocks.

CN121667008APending Publication Date: 2026-03-17TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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Patent Information

Application Number
CN202511766439.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-11-27
Publication Date
2026-03-17

AI Technical Summary

Technical Problem

Existing methods for evaluating the resistance of tomato rootstocks to weevils and root-knot nematodes are not precise enough, leading to biases in the screening of resistant rootstocks and making it impossible to objectively evaluate their resistance levels.

Method used

By combining growth indicators, disease resistance indicators, reproduction coefficients, and soil microbial diversity indices (such as the Simpson index) with membership functions, and using high-throughput sequencing to determine the 16S rRNA gene sequences of soil microorganisms, membership functions and soil microbial diversity indices were calculated to accurately evaluate the rootstock's resistance to *Eriocheir sinensis* root-knot nematode.

Benefits of technology

This study enabled precise evaluation of the resistance of tomato rootstocks to weevils and root-knot nematodes, allowing for better screening of rootstock varieties with strong resistance and good growth, and improving the objectivity and accuracy of resistance evaluation.

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Abstract

The invention belongs to the field of tomato seedling cultivation, and particularly relates to a method for accurately evaluating the level of tomato rootstock resistance to elephantopus scaber root-knot nematode. On the basis of testing indexes such as a disease index, a root knot index, an egg index, a propagation coefficient and a soil microorganism species number of tomato rootstocks, a rootstock growth index is combined, an index (Simpson index) algorithm of species diversity in a soil microorganism community is creatively utilized, evaluation parameters are optimized, and the evaluation accuracy is improved. The measured indexes are adopted to calculate membership functions and soil microbial diversity indexes to evaluate the resistance of the tomato rootstock to be detected to the root-knot nematode, so that the rootstock variety for resisting the tomato rootstock to the root-knot nematode can be better and comprehensively clustered and analyzed, and the resistance of the tomato rootstock to the tomato rootstock to the root-knot nematode can be accurately evaluated; and a novel method is provided for screening the tomato rootstock for resisting the rhus chinensis root-knot nematode.
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