Insect-resistant and herbicide-resistant transgenic maize event JZL22k-1 and detection method thereof

By introducing the cry2Ab, cry1Fa, and cp4epsps genes into the maize genome, the transgenic maize event JZL22k-1 was constructed, and a specific detection method was designed to solve the problems of limited insect resistance spectrum and weed resistance, achieving efficient weed control and pest management, and ensuring the accuracy and stability of detection.

CN121780604APending Publication Date: 2026-04-03ZHEJIANG UNIV
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Patent Information

Application Number
CN202511943634.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-22
Publication Date
2026-04-03

AI Technical Summary

Technical Problem

Existing insect-resistant transgenic maize varieties have limited insect resistance to pests such as fall armyworm and beet armyworm, and long-term use of a single insecticidal protein gene can easily lead to increased pest resistance. Traditional herbicides also lead to increased herbicide resistance in weeds, and existing detection methods cannot accurately distinguish between different transformation events.

Method used

By simultaneously introducing the cry2Ab and cry1Fa genes into the maize genome and combining them with the cp4epsps gene, the transgenic maize event JZL22k-1 was constructed. Specific nucleic acid sequences and primer pairs were designed to achieve accurate detection and identification of JZL22k-1.

Benefits of technology

It improved maize's tolerance to glyphosate herbicide and resistance to major lepidopteran pests, reduced the risk of pest and weed resistance, improved breeding and weed control efficiency, and enabled accurate detection and traceability of JZL22k-1.

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Abstract

The invention discloses an insect-resistant and herbicide-resistant transgenic maize event JZL22k-1 and a detection method of the insect-resistant and herbicide-resistant transgenic maize event JZL22k-1. The transgenic maize event JZL22k-1 nucleic acid sequence comprises SEQ ID NO: 1 or a complementary sequence thereof, and / or SEQ ID NO: 2 or a complementary sequence thereof; the transgenic maize plant JZL22k-1 has good resistance to lepidoptera insects and good tolerance to glyphosate herbicides and has no influence on the yield, and the detection method can accurately and stably identify the existence of a JZL22k-1 transgenic event by applying a nucleic acid amplification method. The method is of great significance in implementation of research of JZL22k-1, traceability of production processing and application and whole-process supervision.
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