Simulation apparatus and method for the effect of salinity stress during freeze-thaw cycles on rhizome germination of emergent plants

By designing a salinity stress simulation device during freeze-thaw cycles, non-destructive monitoring and high-frequency dynamic analysis of emergent plant rhizomes were achieved. This solved the problem of disconnect between the simulation of freeze-thaw cycles, salinity, and nutrients in existing technologies, and provided accurate scientific evidence for the ecological restoration of saline-alkali wetlands.

CN121856492BActive Publication Date: 2026-05-26INNER MONGOLIA AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202610316929.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2026-03-16
Publication Date
2026-05-26
Estimated Expiration
2046-03-16

AI Technical Summary

Technical Problem

Existing technologies cannot achieve precise gradient control and orthogonal combination simulation of the three factors of freeze-thaw, salinity, and nutrients under controllable conditions, resulting in insufficient research on the compound stress tolerance mechanism of emergent plants. Furthermore, traditional experimental methods are difficult to achieve high-frequency, non-destructive monitoring, affecting the accuracy and reliability of experimental data.

Method used

A device was designed to simulate the effect of salinity stress during freeze-thaw cycles on the rhizome germination of emergent plants. Through the structure of a main pool, a closed channel, and a secondary pool, combined with a sliding track and an electromagnet-driven transfer system, the non-destructive transfer and monitoring of plants can be achieved. Combined with an L18 (3²×6¹) orthogonal experimental design, the effects of each factor can be accurately analyzed.

Benefits of technology

It enables non-destructive, in-situ monitoring of emergent plant rhizomes during freeze-thaw cycles, ensuring the stability of the experimental environment, improving the accuracy and reliability of experimental data, supporting high-frequency dynamic monitoring, and providing targeted guidance for the ecological restoration of saline-alkali wetlands.

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Abstract

This invention discloses a device and method for simulating the effects of salinity stress during freeze-thaw cycles on the rhizome germination of emergent plants, belonging to the field of emergent plant germination experiments in cold and arid regions. The device includes an outdoor main pool and a laboratory auxiliary pool, connected by a closed channel with double gates. The bottom of the main and auxiliary pools is equipped with sliding tracks and transfer channels. Within the transfer channels, a translation screw, in conjunction with an electromagnet, drives the base frame to move without breaking ice. The main pool is equipped with freeze-thaw, salinity, and nutrient regulation modules. The method uses an L18 (3²×6¹) orthogonal array to design experimental combinations of freeze-thaw patterns, salinity, and nutrients. The base frame is transferred to the auxiliary pool without breaking ice, and stratified morphological and physiological indicators are measured simultaneously. Data are harvested at the end of the experiment, and variance analysis is performed. This invention achieves undisturbed monitoring of the experimental environment, accurately recreates the complex stress environment, quantifies the effects of the three factors, provides a scientific basis for the ecological restoration of saline-alkali wetlands, and is convenient to operate and highly repeatable.
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