Construction method and application of embryonic development retardation animal model

By constructing an embryonic developmental delay model using fetal bovine serum or FABP4 expression inhibitors and applying DIA proteomics technology, the problems of model instability and analytical difficulties in existing technologies have been solved, achieving model stability and comprehensive analysis of molecular mechanisms, and providing an effective tool for research and drug screening.

CN122038282APending Publication Date: 2026-05-15URUMQI MATERNAL & CHILD HEALTH HOSPITAL
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Patent Information

Application Number
CN202610287208.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-10
Publication Date
2026-05-15

AI Technical Summary

Technical Problem

Existing technologies make it difficult to stably construct models of embryonic developmental delay, and traditional proteomics methods cannot fully elucidate its molecular mechanisms, leading to research difficulties.

Method used

An animal model of embryonic developmental delay was constructed using fetal bovine serum or fatty acid-binding protein 4 expression inhibitors. Combined with data-independent acquisition (DIA) proteomics technology, low-abundance proteins were accurately quantified, and changes in protein expression profiles were comprehensively analyzed.

Benefits of technology

A stable and reliable model of embryonic developmental delay was constructed, with a clear phenotype. This model can comprehensively analyze the multidimensional regulatory mechanisms of embryonic developmental delay, providing an ideal experimental model for research and can be used to screen candidate drugs for the prevention or treatment of embryonic developmental delay.

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Abstract

The invention discloses a construction method and application of an animal model with delayed embryonic development, and relates to the technical field of animal models.The construction method of the animal model with delayed embryonic development comprises the following steps that S1, an early embryo is obtained; s2, performing contact culture on the early embryo and a development retardation inducer; s3, culturing to obtain a slowly developed embryo; the proteomics analysis method based on non-data-dependent acquisition is used for analyzing an animal model with slow embryonic development. The method comprises the following steps: providing an embryo sample; obtaining a protein expression profile by adopting a non-data-dependent acquisition proteomics method; according to a protein expression profile, identifying differential expression proteins related to the embryonic development retardation; the animal model with the delayed embryonic development is constructed, and an experimental model is provided for researching a molecular mechanism of the delayed embryonic development; a non-data-dependent collection proteomics analysis method is applied to the animal model, and compared with a traditional data-dependent collection method, the method has higher stability.
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