Application of HearNPV-miR-hr5b in prevention and treatment of cotton bollworm

By using HearNPV-miR-hr5b microRNA and its agomir to prepare microcapsule or nanoparticle formulations, the problem of cotton bollworm resistance to Bt cotton was solved, the insecticidal speed and efficiency of cotton bollworm nucleopolyhedrovirus were improved, and the control effect on resistant cotton bollworm was enhanced.

CN122128306APending Publication Date: 2026-06-02CHINA AGRI UNIV
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Patent Information

Application Number
CN202610325962.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-17
Publication Date
2026-06-02

AI Technical Summary

Technical Problem

After long-term use, cotton bollworms have developed resistance to Cry1Ac toxin in existing Bt cotton, resulting in a decline in control efficacy. There is a need to develop adjuvants that can improve the insecticidal speed and efficiency of HearNPV to enhance the control effect against resistant cotton bollworms.

Method used

Using HearNPV-miR-hr5b microRNA and its agomir, and through the modified nucleotide sequence CGGGCAAATTTCAAATAAA, microcapsules or nanoparticle formulations were prepared and mixed with bollworm nucleopolyhedrovirus for application, which increased the replication accumulation and infection rate of the virus in bollworm.

Benefits of technology

It significantly accelerates the mortality rate of cotton bollworms, improves the insecticidal effect on wild and resistant cotton bollworms, enhances the virus's ability to spread, and improves control efficiency.

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Abstract

The invention belongs to the field of biological pesticides, and provides application of HearNPV-miR-hr5b in prevention and treatment of cotton bollworms, and the nucleotide sequence of the HearNPV-miR-hr5b is CGGGCAAAUUUCAAAUAAA. The HearNPV-miR-hr5b provided by the invention can improve the replication and accumulation of HearNPV in host cotton bollworms, accelerate the death rate of larvae and improve the rate of resisting the resistant cotton bollworms, can be used for developing a HearNPV synergist, and has a good application prospect in the aspect of biological prevention and control of the cotton bollworms.
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