Preparation method of bionic temperature-sensitive hydrogel loaded with adipose-derived stem cell exosome

By constructing microencapsulated exosomes combined with thermosensitive hydrogels, the problems of rapid clearance and controlled release of exosomes in vivo were solved, achieving long-term sustained release of exosomes and a suitable microenvironment to promote tissue repair.

CN122163531APending Publication Date: 2026-06-09JIAXING UNIV
1 Cites 0 Cited by

Patent Information

Application Number
CN202610326290.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-17
Publication Date
2026-06-09

AI Technical Summary

Technical Problem

In existing technologies, exosomes are easily cleared by the immune system when applied in vivo, have a short half-life, lack effective controlled-release carriers, and hydrogels are difficult to simulate the natural extracellular matrix, resulting in low local retention rate and loss of activity, making it difficult to match the growth rate of new tissue.

Method used

A layer-by-layer self-assembly technique is used to encapsulate adipose-derived stem cell exosomes to form microencapsulated exosomes, which are then combined with amino-modified temperature-responsive polymers and oxidatively modified natural polysaccharides. Through temperature-induced physical cross-linking and dynamic covalent bonds, a biomimetic temperature-sensitive hydrogel is formed, achieving long-term sustained release of exosomes and a suitable microenvironment.

Benefits of technology

The hydrogel solidifies rapidly at body temperature, providing a suitable microenvironment, prolonging the retention time of exosomes, improving treatment efficacy, and promoting wound healing and angiogenesis.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention relates to the technical field of biomedical materials and regenerative medicine, in particular to a preparation method of adipose-derived stem cell exosome-loaded bionic temperature-sensitive hydrogel, which comprises the following steps: preparing an aminated temperature-sensitive polymer; preparing oxidation modified natural polysaccharide; wrapping the adipose-derived stem cell exosome in a polyelectrolyte multilayer film by adopting a layer-by-layer self-assembly technology to form a microencapsulated exosome; the aminated temperature-sensitive polymer, the oxidized modified natural polysaccharide and the microencapsulated exosome are mixed at low temperature, and the temperature-sensitive hydrogel with a bionic structure is cured through temperature-induced physical crosslinking and the synergistic effect of dynamic Schiff base bonds formed by amino and aldehyde groups. The hydrogel prepared by the invention has good temperature-sensitive injectability, mechanical property and self-healing ability, the bionic multilayer structure of the hydrogel effectively simulates a natural extracellular matrix microenvironment, and long-acting slow release and activity protection of the exosome are realized through a'gel-microcapsule 'multistage barrier system.
Need to check novelty before this filing date? Find Prior Art