A seed treatment agent based on 2,6-pyridinedicarboxylic acid or bacillus subtilis high-dpa and its application

By using a seed treatment agent containing high-yield 2,6-pyridinedicarboxylic acid and high-yield DPA Bacillus subtilis MT strain, the problems of insufficient colonization capacity and unstable promotion effect in the existing technology have been solved, achieving significant promotion of seed germination and seedling growth, and possessing the advantages of being environmentally friendly and easy to operate.

CN122207702APending Publication Date: 2026-06-16NANTONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANTONG UNIV
Filing Date
2026-03-30
Publication Date
2026-06-16

AI Technical Summary

Technical Problem

Existing Bacillus subtilis seed treatment agents have limited colonization ability on seed surfaces and mainly work by inhibiting pathogens. They lack active ingredients that directly promote seed germination and seedling growth, resulting in unstable application effects.

Method used

High-yielding 2,6-pyridinedicarboxylic acid (DPA) and high-DPA-yielding Bacillus subtilis MT strains were used as seed treatment agents to promote seed germination and seedling growth by regulating the balance of endogenous hormones in seeds, and to enhance the colonization ability of the strains on the seed surface by expressing the spoVF operon.

Benefits of technology

It significantly promotes seed germination and seedling growth, significantly improving germination rate and growth indicators. It is also environmentally friendly, with no risk of chemical pesticide residues, and is easy to operate and promote.

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Abstract

The application discloses a seed treatment agent based on 2,6-pyridinedicarboxylic acid or a high-DPA bacillus subtilis and application thereof, and is used for promoting seed germination and seedling growth, wherein DPA can directly promote seed germination and seedling growth as a signal molecule, and the MT strain with high DPA production has excellent colonization capacity on the seed surface and can continuously secrete DPA in situ; after the seed treatment agent is used, the germination rate of barley seeds is increased from 92% to 98%, the radicle length is increased from 1.0 cm to 4.1 cm, and the plumule length is increased from 2.1 cm to 7.2 cm; the germination rate of soybean seeds is increased from 86% to 96%, the taproot length is increased by 37%, and the fresh weight of seedlings is increased by 45%, and the application is simple in operation, safe and environment-friendly, and provides a new safe, efficient and environment-friendly strategy for seed treatment.
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