Preparation and application method of traditional Chinese medicine compound preparation for preventing and controlling areca yellowing disease in green way

Abstract

The application discloses a preparation and application method of a traditional Chinese medicine compound preparation for preventing and controlling areca yellowing disease, relates to the field of green prevention and control technology of tropical economic crop diseases in modern agriculture and biotechnology, and realizes chemical pesticide residue-free and green and safe prevention and control by using traditional Chinese medicine to replace chemical pesticides. Meanwhile, the traditional Chinese medicine compound preparation can inhibit pathogenic phytoplasma / virus, control medium insects, repair root systems and soil microecology, solve the areca yellowing disease problem for a long time, improve the immunity of areca, promote the yellowing areca to return to green, prolong the effective period, and reduce the recurrence rate.

Description

Technical Field

[0001] This invention relates to the field of green control technology for tropical economic crop diseases in modern agriculture and biotechnology, specifically a method for preparing and applying a traditional Chinese medicine compound preparation for green control of areca nut yellowing disease. Background Technology

[0002] Areca nut is the top of the four major southern Chinese medicinal herbs. In the past decade, areca nut yellowing disease has been spreading and worsening in Hainan, the main producing area, directly affecting areca nut yield and quality, and further impacting the economic benefits of areca nut growers. Areca nut yellowing disease is caused by a complex infection of phytoplasma and virus, including *Areca Palm Yellow Leaf Phytoplasma* (AYLP) and *Areca Palm Velarivirus 1* (APV1), coupled with the transmission of the virus by insects such as the black spiny whitefly, the bifurcate whitefly, and the long-tailed mealybug, the transmission of phytoplasma by leafhoppers, soil microecological imbalance, and root system decline, resulting in a devastating disease that has caused large-scale yield reductions and tree death in areca nut producing areas of Hainan and Yunnan.

[0003] The mainstream control methods in the current technology are: chemical pesticide control: frequent application of fungicides, insecticides, and copper preparations easily leads to resistance, pesticide residues, soil compaction, and damage to natural enemies and micro-ecology, making it difficult to eradicate and not in line with the requirements of green planting; physical / agricultural measures: removing diseased plants, cleaning the garden, and adjusting water and fertilizer, which are slow to take effect, have a short control window, and have poor effects on restoring greening of already diseased plants; biological agents: single bacterial agents and immune inducers have insufficient stability and short duration of action, and have limited effect on controlling yellowing disease caused by multiple infections.

[0004] However, the aforementioned technologies have the following drawbacks: Chemical control: excessive chemical pesticide residues, increased pesticide resistance in pests and diseases, and damage to the ecological environment, failing to meet organic / green areca nut production standards; Single biological control: weak targeting and poor synergistic effects, making it difficult to simultaneously achieve sterilization and toxicity suppression, control of vector pests, strengthening of root systems, improvement of immunity, and soil repair and improvement; Lack of a mature traditional Chinese medicine compound system: lacking targeted compounding of medicinal and edible herbs, and integrated application technology that combines control and nutritional repair. Therefore, this invention provides a method for preparing and applying a traditional Chinese medicine compound preparation for the green control of areca nut yellowing disease. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides a method for the preparation and application of a traditional Chinese medicine compound preparation for the green prevention and control of areca nut yellowing disease, thereby solving the aforementioned problems.

[0006] To achieve the above objectives, the present invention is implemented through the following technical solution: a method for preparing a traditional Chinese medicine compound preparation for green control of areca nut yellowing disease, comprising: antibacterial and antiviral components, insect repellent components, root-promoting and immune-inducing components, and adjuvants;

[0007] The antibacterial and antiviral components include the following raw materials in parts: 10-15 parts Coptis chinensis, 8-12 parts Phellodendron chinense, 8-12 parts Isatis indigotica, and 6-10 parts Lonicera japonica.

[0008] The insect repellent component comprises the following ingredients in parts: 10-15 parts Sophora flavescens, 6-10 parts Neem, 6-9 parts Rhizoma Ctenoptera, and 5-12 parts Artemisia argyi.

[0009] The root-promoting immune-inducing component comprises the following raw materials in parts: 8-12 parts of Astragalus membranaceus, 5-8 parts of Glycyrrhiza uralensis, and 3-6 parts of humic acid;

[0010] The additives include the following raw materials in parts: 1-3 parts of saponin penetrant and 1-2 parts of polysaccharide stabilizer.

[0011] Preferably, the antibacterial and antiviral components include the following raw materials in parts: 12 parts Coptis chinensis, 10 parts Phellodendron chinense, 10 parts Isatis indigotica, and 6 parts Lonicera japonica.

[0012] The insect repellent component comprises the following ingredients in parts: 12 parts Sophora flavescens, 6 parts Neem, 6 parts Rhizoma Ctenoptera, and 9 parts Artemisia argyi.

[0013] The root-promoting immune-inducing component comprises the following raw materials in parts: 8 parts Astragalus membranaceus, 5 parts Glycyrrhiza uralensis, and 5 parts Humic acid;

[0014] The additives include the following raw materials in parts: 2 parts saponin penetrant and 2 parts polysaccharide stabilizer.

[0015] Preferably, the antibacterial and antiviral components include the following raw materials in parts: 15 parts Coptis chinensis, 10 parts Phellodendron chinense, 8 parts Isatis indigotica, and 8 parts Lonicera japonica.

[0016] The insect repellent component comprises the following ingredients in parts: 15 parts Sophora flavescens, 10 parts Neem, 8 parts Rhizoma Ctenoptera, and 5 parts Artemisia argyi.

[0017] The root-promoting immune-inducing component comprises the following ingredients in parts: 10 parts Astragalus membranaceus, 5 parts Glycyrrhiza uralensis, and 4 parts Humic acid;

[0018] The additives include the following raw materials in parts: 2 parts saponin penetrant and 2 parts polysaccharide stabilizer.

[0019] Preferably, the procedure includes the following steps:

[0020] Step 1: Preparation of raw Chinese medicinal materials and processed Chinese medicinal slices: Prepare raw Chinese medicinal materials and processed Chinese medicinal slices according to their components;

[0021] Step 2, Mixing: Place the prepared raw Chinese medicine and processed medicinal materials into a mixer for mixing;

[0022] Step 3, Grinding: Place the mixed raw Chinese medicine and processed Chinese medicine into a grinder and grind them separately, then sieve them through a 60-80 mesh screen;

[0023] Step 4: Boil with water: Place the crushed and sieved raw materials into a boiling device, add 8 to 12 times the amount of water, and boil.

[0024] Step 5: Extraction of traditional Chinese medicine compound preparations: Place the raw materials after decoction into the extraction equipment and extract 3 to 5 times at a temperature of 90 to 100℃, each time for 1.5 to 2 hours;

[0025] Step 6, Cooling: Transfer the extracted liquid to a stainless steel cooling tank and allow it to cool naturally or with circulating water to room temperature. Stir every 15 minutes during the cooling process. After cooling, perform coarse filtration through a 300-mesh filter cloth to remove the residue and insoluble impurities, and obtain the preliminary filtrate.

[0026] Step 7, Negative pressure filtration: Transfer the preliminary filtrate to a centrifuge to remove fine suspended particles and obtain a clear filtrate;

[0027] Step 8: Concentrate the original Chinese medicine liquid: Place the obtained clear filtrate in a vacuum concentrator and concentrate it under reduced pressure until the solid content is 5% to 8%;

[0028] Step 9: Compound preparation of traditional Chinese medicine: Add adjuvants, stir to dissolve, homogenize, sterilize, and fill.

[0029] Preferably, the mixer is a double-helix conical mixer with a mixing speed of 20-30 r / min and a mixing time of 15-25 minutes.

[0030] Preferably, the crusher is a hammer mill, and the feeding speed is controlled at 100-150 kg / h during crushing. After crushing, the material passes through a 70-mesh sieve.

[0031] Preferably, the cooking equipment is a stainless steel jacketed kettle, which is kept at a gentle boil during the cooking process and stirred once every 30 minutes.

[0032] Preferably, the extraction equipment is a multi-functional extraction tank, and the pressure inside the tank is maintained at 0.05-0.1 MPa during the extraction process, with the extraction liquid circulating at a flow rate of 2-3 m / s. 3 / h.

[0033] Preferably, the centrifuge is used to centrifuge at a speed of 3000 r / min for 15 minutes.

[0034] A method for green control of areca nut yellowing disease using a traditional Chinese medicine compound preparation, comprising any one of the above-mentioned traditional Chinese medicine compound preparations, characterized in that it includes:

[0035] Disease prevention period (new shoots / flowering period): Dilute the Chinese herbal compound preparation 300-500 times and spray on the leaves, once every 10-15 days, for 2-3 consecutive times;

[0036] In the early stage of the disease: dilute 200-300 times for spraying and 500-800 times for root irrigation, 5-10 kg per plant per irrigation, once every 10 days, for 2-3 consecutive applications;

[0037] Rejuvenation and restoration period: Combine with drip irrigation / fertigation of organic fertilizer to improve the root zone and enhance tree vigor.

[0038] Beneficial effects

[0039] Compared with the prior art, the present invention has the following advantages:

[0040] 1. The innovative combination of traditional Chinese medicine compound formulations for the first time combines antibacterial, antiviral, vector-repelling, and immune-boosting herbs to synergistically control areca nut yellowing disease caused by multiple infections; reflecting the principle of syndrome differentiation and treatment.

[0041] 2. Traditional Chinese medicine replaces chemical pesticides, is green, safe and effective, contains all biological components of traditional Chinese medicine, has no chemical pesticide residues, and meets the requirements for green food / organic areca nut production;

[0042] 3. Innovative application method: integrating spraying and root irrigation, it provides green control of leaf diseases and vector insects on areca trees, and repairs the root system and soil underground, thus strengthening the foundation and treating both the symptoms and the root cause.

[0043] 4. Stable effect: Long-lasting effect, high regreening rate of areca nut diseased plants, reduced recurrence rate, and improved yield and quality. Attached Figure Description

[0044] Figure 1 This is a flowchart of the preparation process of the present invention. Detailed Implementation

[0045] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0046] A method for preparing a traditional Chinese medicine compound for green control of areca nut yellowing disease, comprising: antibacterial and antiviral components, insect repellent components, root-promoting and immune-inducing components, and adjuvants;

[0047] The antibacterial and antiviral components include the following raw materials in parts: 10-15 parts Coptis chinensis, 8-12 parts Phellodendron chinense, 8-12 parts Isatis indigotica, and 6-10 parts Lonicera japonica.

[0048] Coptis chinensis possesses broad-spectrum antibacterial and antiviral activity. Its main active ingredient, berberine, can specifically inhibit the growth and reproduction of pathogenic fungi related to areca nut yellowing disease (such as Fusarium oxysporum and anthracnose), destroy the integrity of the pathogen's cell membrane, and interfere with its nucleic acid and protein synthesis and metabolism. At the same time, it also has a significant inhibitory effect on viral pathogens that spread yellowing disease, and can effectively block the replication and spread of viruses in the plant, providing an early disease defense barrier for areca nut trees.

[0049] Phellodendron bark contains abundant alkaloid active ingredients (such as berberine, berberine, and palmatine), which can not only synergize with Coptis chinensis to enhance the broad-spectrum antibacterial effect, but also effectively inhibit hyphal elongation and spore germination, and disrupt the cellular structural stability of pathogenic fungi such as Phytophthora and Pythium associated with areca nut yellowing disease. At the same time, its active ingredients can interfere with the energy metabolism and enzymatic reactions of pathogens, reducing their infectivity. In addition, Phellodendron bark can also induce a significant increase in the activity of defense enzymes such as peroxidase and phenylalanine ammonia-lyase in areca nut trees, activate the plant's own immune response mechanism, and synergistically construct a multi-layered disease defense network with other antibacterial and antiviral components, laying a good foundation for the subsequent rejuvenation and revegetation stage.

[0050] Isatis root is rich in indole alkaloids (such as indigo and indirubin) and polysaccharide active ingredients. Its alkaloid components can specifically inhibit the proliferation of viruses related to areca nut yellowing disease (such as areca nut necrosis ringspot virus), blocking systemic transmission of the virus within the areca nut tree by interfering with viral nucleic acid replication and protein coat assembly. Simultaneously, its polysaccharide components can significantly activate the non-specific immune response of the areca nut tree, promoting the synthesis and activity of disease-resistant enzymes such as chitinase and β-1,3-glucanase, thus strengthening the plant's resistance to pathogens. Furthermore, the synergistic effect of Isatis root with the active ingredients of Coptis chinensis and Phellodendron amurense can further broaden the antibacterial and antiviral spectrum, showing a certain inhibitory effect on pathogenic fungi such as Fusarium oxysporum and Anthracnose. Together with other antibacterial and antiviral components, it constitutes a comprehensive disease control system, providing multiple safeguards for areca nut trees against yellowing disease.

[0051] Honeysuckle is rich in chlorogenic acid, luteolin, and flavonoid glycosides. Its chlorogenic acid component can significantly reduce the infectivity of areca nut yellowing disease-related pathogens (such as Fusarium oxysporum and Anthracnose) by disrupting the cell membrane permeability, inhibiting hyphal elongation and spore germination. At the same time, flavonoid components such as luteolin have a strong inhibitory effect on viral pathogens that transmit yellowing disease, interfering with the assembly and release of viral particles and blocking the horizontal and vertical spread of viruses in areca nut plants. In addition, the synergistic effect of honeysuckle with the active components of Coptis chinensis, Phellodendron chinense, and Isatis indigotica can further expand the scope of antibacterial and antiviral activity, and also show good inhibitory effects on other related pathogens such as Phytophthora. Its organic acid components can also induce the activity of defense enzymes such as superoxide dismutase and peroxidase in areca nut trees, enhance the plant's antioxidant capacity and immune response level, and work with other components to build a three-dimensional disease defense barrier, providing continuous protection for the healthy growth of areca nut trees.

[0052] The insect repellent component includes the following ingredients in parts: 10-15 parts Sophora flavescens, 6-10 parts Neem, 6-9 parts Rhizoma Ctenoptera, and 5-12 parts Artemisia argyi.

[0053] Sophora flavescens possesses abundant alkaloid active ingredients (such as matrine, oxymatrine, and sophoridine). Its core active ingredient, matrine, has significant repellent and antifeedant effects on insects that transmit areca nut yellowing disease (such as areca leafhoppers, aphids, and whiteflies). Matrine acts on the nervous system of these insects, inhibiting acetylcholinesterase activity, interfering with nerve signal transmission, leading to muscle paralysis, slowed movement, and even death. Simultaneously, it damages the digestive tract mucosa of insects, affecting nutrient absorption and metabolic function, reducing the survival and reproductive capacity of these insects. Furthermore, the synergistic effect of Sophora flavescens with repellent components from neem and rotenone root further broadens the insecticidal spectrum, enhancing the control effect against different insect vectors, effectively cutting off the transmission chain of yellowing disease pathogens through insect vectors, and providing crucial support for areca nut trees to block disease infection sources.

[0054] Neem contains abundant tetracyclic triterpenoid active ingredients (such as azadirachtin A and azadirachtin B). Its core active ingredient, azadirachtin, has a strong antifeeding, oviposition-inhibiting, and growth-disrupting effect on insect vectors that transmit areca nut yellowing disease (such as areca leafhoppers, whiteflies, and aphids). Azadirachtin can mimic the juvenile hormone signals in insects, interfering with their normal molting and metamorphosis processes, leading to larvae failing to pupate successfully, adult morphological deformities, or impaired reproductive function, significantly reducing the reproductive capacity of vector insect populations. At the same time, it can act on the taste and olfactory receptors of insects, inhibiting their feeding behavior and reducing the piercing-sucking damage to areca nut trees by vector insects, thereby cutting off the direct route of pathogen transmission through insect vectors. In addition, when neem works synergistically with other repellent components such as sophora flavescens and rotenone root, it can enhance the repellent effect on vector insects, especially against the highly resistant areca leafhopper population, significantly improving the control effect, further consolidating the blocking line of disease transmission chain, and creating a healthy growth environment for areca trees free from insect interference.

[0055] Rotenone root is rich in flavonoid active ingredients (such as rotenone and rotenoidin). Its core active ingredient, rotenone, has highly effective contact, stomach poison, and antifeedant effects on insect vectors that transmit areca nut yellowing disease (such as areca leafhoppers, aphids, and whiteflies). Rotenone can inhibit the activity of respiratory chain complex I in insect cell mitochondria, blocking the production of energy ATP, leading to rapid death of insects due to energy metabolism disorders. At the same time, it can act on the insect digestive system, damaging the intestinal cell structure, affecting the absorption of nutrients, and further reducing the survival ability of vector insects. In addition, when rotenone root works synergistically with other insecticidal components such as Sophora flavescens and Neem, they can complement each other's insecticidal mechanisms, broaden the insecticidal spectrum, and improve the control effect on drug-resistant vector insects, effectively cutting off the key pathway of yellowing disease pathogen transmission through insect vectors, and building a solid insect vector barrier for areca nut trees.

[0056] Artemisia argyi is rich in volatile oils (such as eucalyptol, thujone, and borneol) and flavonoid active ingredients. Its volatile oil components have significant repellent, contact-killing, and fumigation effects on insects that transmit areca nut yellowing disease (such as areca leafhoppers, aphids, and whiteflies). Eucalyptol in the volatile oils acts on the olfactory receptors of these insects, producing a strong repellent effect and reducing their approach to and sucking behavior towards areca nut trees. Thujone and other components have contact-killing activity, destroying the waxy layer on the insect's body surface, interfering with its water balance and nerve signal transmission, leading to rapid insect death. Simultaneously, the fumigation effect of Artemisia argyi volatile oils can enter the insect's body through the respiratory tract, inhibiting the activity of its respiratory metabolic enzymes, blocking energy supply, and further enhancing its insecticidal efficacy. In addition, when Artemisia argyi works synergistically with other repellent components such as Sophora flavescens, Neem, and Rhizoma et Radix, it can enhance the control effect on vector insects through multiple mechanisms. In particular, it can penetrate into the leaf crevices through fumigation to exert its effect on leaf nymphs, which are highly concealed. This effectively supplements the control blind spots of other components, further cuts off the insect vector transmission chain of chlorosis pathogens, and builds a more comprehensive insect vector defense system for Areca catechu trees.

[0057] The root-promoting immune-inducing component includes the following raw materials in parts: Astragalus membranaceus 8-12 parts, Glycyrrhiza uralensis 5-8 parts, and humic acid 3-6 parts;

[0058] Astragalus contains abundant polysaccharides (such as astragalus polysaccharide) and saponins (such as astragaloside I and II), with its core active ingredient, astragalus polysaccharide, significantly promoting root development in areca palms. It stimulates root cell division and elongation, increases the number of lateral roots and root hair density, enhances the root system's ability to absorb soil moisture, nitrogen, phosphorus, potassium, and other nutrients, strengthens the nutritional foundation of areca palms, and provides solid physiological support for the plant's resistance to chlorosis. Simultaneously, astragalus polysaccharide can activate the activity of defense enzymes such as superoxide dismutase and phenylalanine ammonia-lyase in areca palms, promote the synthesis of disease-resistant substances such as phytoalexins and lignin, enhance the plant's non-specific immune response, and effectively improve its resistance to chlorosis pathogens. In addition, when Astragalus works synergistically with licorice and humic acid, Astragalus saponins can complement glycyrrhizic acid in licorice, further enhancing the plant's stress resistance. When combined with humic acid, it can improve the soil microenvironment, promote the reproduction of beneficial microorganisms, indirectly optimize the root health status, and jointly build a synergistic network of root promotion and immune induction, providing continuous impetus for the rejuvenation and greening of areca palms.

[0059] Licorice is rich in triterpenoid saponins (such as glycyrrhizic acid and glycyrrhetinic acid) and flavonoids (such as glycyrrhizin and isoglycyrrhizin), with its core active ingredient, glycyrrhizic acid, playing a crucial role in the immune regulation and nutritional balance of areca palms. Glycyrrhizic acid can regulate hormone levels in plants, promote the synergistic effect of cytokinins and auxins, enhance leaf photosynthetic efficiency, and increase the accumulation of organic matter. Simultaneously, it can inhibit pathogen-related molecular pattern signaling, reduce the plant's sensitivity to chlorosis pathogens, and strengthen the immune defense response. Furthermore, when licorice is used synergistically with astragalus and humic acid, glycyrrhizin flavonoids can complement astragalus polysaccharides, further enhancing the immune-inducing effect; when combined with humic acid, it can chelate heavy metal ions in the soil, reducing their toxicity to the roots, thus jointly maintaining a healthy growth environment for areca palms and providing multi-dimensional protection against chlorosis.

[0060] Humic acid is rich in humic acid, fulvic acid, and humate active ingredients. Its core active ingredient, fulvic acid, plays a crucial role in soil improvement and root protection for areca palms. Fulvic acid can improve soil aggregate structure, enhance soil water and fertilizer retention capacity, promote the reproduction of beneficial microorganisms around the roots (such as rhizobia and Bacillus), and inhibit the growth of pathogenic fungi. Simultaneously, it can chelate trace elements in the soil (such as iron, zinc, and manganese), enhancing their bioavailability and alleviating chlorosis symptoms in areca palms caused by nutrient deficiencies. Furthermore, when humic acid works synergistically with astragalus and licorice, it can enhance the root system's absorption efficiency of nutrients, activate the plant's antioxidant enzyme system, further improve immune resistance, and jointly construct a comprehensive system for promoting root growth, improving soil, and enhancing immunity, providing all-round ecological support for the prevention and control of chlorosis in areca palms.

[0061] The additives include the following raw materials in parts: 1-3 parts of saponin penetrant and 1-2 parts of polysaccharide stabilizer.

[0062] Saponin penetrants contain natural saponin active ingredients (such as saponins and tea saponins), and their core function is to optimize the interfacial properties and penetration efficiency of the drug solution. They can significantly reduce the surface tension of the drug solution, enhance its wettability and adhesion on the surfaces of areca nut leaves, stems, and roots, effectively break down the physical barriers of the plant's epidermal cuticle, waxy layer, and root cell walls, and promote the rapid penetration of active ingredients in antibacterial, antiviral, insect-repelling, and root-promoting immune-inducing components into target sites within the plant (such as leaf palisade tissue, stem vascular bundles, and root absorption zones), greatly improving the bioavailability of each functional component. Simultaneously, when saponin penetrants synergistically work with active ingredients such as berberine and indigo carmine in the antibacterial and antiviral components, they can enhance their accumulation concentration at pathogen infection sites, thereby strengthening the antibacterial and antiviral effects. When combined with matrine and azadirachtin in the insect-repelling components, they can improve the adhesion and penetration of the pesticide solution on the insect surface, accelerating the action of the insecticidal active ingredients. Furthermore, when combined with astragalus polysaccharides and humic acid in the root-promoting and immune-inducing components, they help these components to be absorbed and utilized by the root system more quickly, further promoting root development and immune response. In addition, saponin penetrants are derived from natural plant extracts, have good environmental compatibility, and pose no residue risk, meeting the requirements of green pest control and providing key technical support for the efficient application of traditional Chinese medicine compound preparations.

[0063] Polysaccharide stabilizers possess natural polysaccharide active ingredients (such as chitosan and gum arabic), and their core function is to maintain the structural stability and long-lasting activity of each active ingredient in compound formulations. They can form stable complexes with active ingredients in antibacterial, antiviral, insecticidal, and root-promoting components through hydrogen bonding and hydrophobic interactions, effectively preventing degradation, oxidation, or precipitation of active ingredients during storage and application, thus extending the shelf life and field efficacy of the formulation. Simultaneously, polysaccharide stabilizers can form a breathable and water-retaining protective film on the surface of areca palm plants, slowing the volatilization and loss of active ingredients in the pesticide solution, continuously releasing effective components, and enhancing the persistence of control effects. When working synergistically with saponin penetrants, they can also enhance the adhesion of the pesticide solution to the plant surface, further optimizing penetration efficiency. Furthermore, polysaccharide stabilizers have good biodegradability and will not pollute the soil or environment, highly aligning with the green control concept of traditional Chinese medicine compound formulations, providing a reliable guarantee for the stable application and long-lasting effect of the formulation.

[0064] Please see Figure 1 A method for preparing a traditional Chinese medicine compound for the green control of areca nut yellowing disease includes the following steps:

[0065] Step 1: Preparation of raw Chinese medicinal materials and processed Chinese medicinal slices: Prepare raw Chinese medicinal materials and processed Chinese medicinal slices according to their components;

[0066] Step 2, Mixing: Place the prepared raw Chinese medicine and processed medicinal materials into a mixer for mixing;

[0067] Step 3, Grinding: Place the mixed raw Chinese medicine and processed Chinese medicine into a grinder and grind them separately, then sieve them through a 60-80 mesh screen;

[0068] Step 4: Boil with water: Place the crushed and sieved raw materials into a boiling device, add 8 to 12 times the amount of water, and boil.

[0069] Step 5: Extraction of traditional Chinese medicine compound preparations: Place the raw materials after decoction into the extraction equipment and extract 3 to 5 times at a temperature of 90 to 100℃, each time for 1.5 to 2 hours;

[0070] Step 6, Cooling: Transfer the extracted liquid to a stainless steel cooling tank and allow it to cool naturally or with circulating water to room temperature. Stir every 15 minutes during the cooling process. After cooling, perform coarse filtration through a 300-mesh filter cloth to remove the residue and insoluble impurities, and obtain the preliminary filtrate.

[0071] Step 7, Negative pressure filtration: Transfer the preliminary filtrate to a centrifuge to remove fine suspended particles and obtain a clear filtrate;

[0072] Step 8: Concentrate the original Chinese medicine liquid: Place the obtained clear filtrate in a vacuum concentrator and concentrate it under reduced pressure until the solid content is 5% to 8%;

[0073] Step 9: Compound preparation of traditional Chinese medicine: Add adjuvants, stir to dissolve, homogenize, sterilize, and fill.

[0074] Specifically, a double-spiral conical mixer is selected, with a mixing speed of 20-30 r / min and a mixing time of 15-25 minutes to ensure that the raw materials are mixed evenly without dead corners.

[0075] Specifically, a hammer mill is selected as the crusher. The feeding speed is controlled at 100-150 kg / h during crushing. After crushing, the material is passed through a 70-mesh sieve to ensure uniform particle size.

[0076] Specifically, a stainless steel jacketed kettle is used for cooking, and a gentle boil is maintained during the cooking process. The kettle is stirred every 30 minutes to prevent the raw materials from sticking to the pot.

[0077] Specifically, a multi-functional extraction tank is selected as the extraction equipment. During the extraction process, the pressure inside the tank is maintained at 0.05 to 0.1 MPa, and the circulation flow rate of the extract is 2 to 3 m3 / h to promote the full dissolution of the active ingredients.

[0078] Specifically, the centrifuge is set to 3000 r / min for 15 minutes. This setting can effectively separate the fine suspended particles and colloidal impurities remaining in the initial filtrate, ensuring that the filtrate clarity reaches more than 98%. This not only reduces the adsorption and deposition of impurities during the subsequent vacuum concentration process and reduces the difficulty of equipment cleaning, but also improves the purity and stability of the final formulation, providing a guarantee for the uniform dissolution of adjuvants and the performance of the formulation in the compounding process.

[0079] A method for the green control of areca nut yellowing disease using a traditional Chinese medicine compound preparation, comprising any one of the above-mentioned traditional Chinese medicine compound preparations, including:

[0080] Disease prevention period (new shoots / flowering period): Dilute the Chinese herbal compound preparation 300-500 times and spray on the leaves, once every 10-15 days, for 2-3 consecutive times;

[0081] In the early stage of the disease: dilute 200-300 times for spraying and 500-800 times for root irrigation, 5-10 kg per plant per irrigation, once every 10 days, for 2-3 consecutive applications;

[0082] Rejuvenation and restoration period: Combine with drip irrigation / fertigation of organic fertilizer to improve the root zone and enhance tree vigor.

[0083] Example 1 (Preventative)

[0084] A method for preparing a traditional Chinese medicine compound for green control of areca nut yellowing disease, comprising: antibacterial and antiviral components, insect repellent components, root-promoting and immune-inducing components, and adjuvants;

[0085] The antibacterial and antiviral components include the following ingredients in parts: 12 parts Coptis chinensis, 10 parts Phellodendron chinense, 10 parts Isatis indigotica, and 6 parts Lonicera japonica.

[0086] The insect repellent component includes the following ingredients in parts: 12 parts Sophora flavescens, 6 parts Neem, 6 parts Rhizoma et Radix et Rhizoma, and 9 parts Artemisiae argyi.

[0087] The root-promoting immune-inducing component includes the following ingredients in parts: Astragalus membranaceus 8 parts, Glycyrrhiza uralensis 5 parts, and Humic acid 5 parts;

[0088] The additives include the following raw materials in parts: 2 parts saponin penetrant and 2 parts polysaccharide stabilizer.

[0089] Process: Extracted three times at 95℃, concentrated to 6% solids;

[0090] Usage: Apply a 400-fold dilution of the prepared Chinese herbal compound preparation as a foliar spray once every 12 days. The control efficacy is 86%.

[0091] Example 2 (Therapeutic)

[0092] A method for preparing a traditional Chinese medicine compound for green control of areca nut yellowing disease, comprising: antibacterial and antiviral components, insect repellent components, root-promoting and immune-inducing components, and adjuvants;

[0093] The antibacterial and antiviral components include the following ingredients in parts: 15 parts Coptis chinensis, 10 parts Phellodendron chinense, 8 parts Isatis indigotica, and 8 parts Lonicera japonica.

[0094] The insect repellent component includes the following ingredients in parts: 15 parts Sophora flavescens, 10 parts Neem, 8 parts Rhizoma Ctenoptera, and 5 parts Artemisia argyi.

[0095] The root-promoting immune-inducing component includes the following ingredients in parts: 10 parts Astragalus membranaceus, 5 parts Glycyrrhiza uralensis, and 4 parts Humic acid;

[0096] The additives include the following raw materials in parts: 2 parts saponin penetrant and 2 parts polysaccharide stabilizer.

[0097] Process: Extracted three times at 95℃, concentrated to 6% solids;

[0098] Usage: Spray with 250 times the prepared Chinese herbal compound preparation concentrate and drench the roots with 600 times the concentrate, once every 10 days, for a total of 3 applications. The greening rate is 82% and the disease index is reduced by more than 70%.

[0099] Furthermore, any content not described in detail in this specification is existing technology known to those skilled in the art.

[0100] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0101] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A method for preparing a traditional Chinese medicine compound for the green control of areca nut yellowing disease, comprising: Antibacterial and antiviral components, insect repellent components, root-promoting and immune-inducing components, and adjuvants; characterized in that, The antibacterial and antiviral components include the following raw materials in parts: 10-15 parts Coptis chinensis, 8-12 parts Phellodendron chinense, 8-12 parts Isatis indigotica, and 6-10 parts Lonicera japonica. The insect repellent component comprises the following ingredients in parts: 10-15 parts Sophora flavescens, 6-10 parts Neem, 6-9 parts Rhizoma Ctenoptera, and 5-12 parts Artemisia argyi. The root-promoting immune-inducing component comprises the following raw materials in parts: 8-12 parts of Astragalus membranaceus, 5-8 parts of Glycyrrhiza uralensis, and 3-6 parts of humic acid; The additives include the following raw materials in parts: 1-3 parts of saponin penetrant and 1-2 parts of polysaccharide stabilizer.

2. The preparation method of a traditional Chinese medicine compound preparation for green control of areca nut yellowing disease according to claim 1, characterized in that, The antibacterial and antiviral components include the following ingredients in parts: 12 parts Coptis chinensis, 10 parts Phellodendron chinense, 10 parts Isatis indigotica, and 6 parts Lonicera japonica. The insect repellent component comprises the following ingredients in parts: 12 parts Sophora flavescens, 6 parts Neem, 6 parts Rhizoma Ctenoptera, and 9 parts Artemisia argyi. The root-promoting immune-inducing component comprises the following raw materials in parts: 8 parts Astragalus membranaceus, 5 parts Glycyrrhiza uralensis, and 5 parts Humic acid; The additives include the following raw materials in parts: 2 parts saponin penetrant and 2 parts polysaccharide stabilizer.

3. The preparation method of a traditional Chinese medicine compound preparation for green control of areca nut yellowing disease according to claim 1, characterized in that, The antibacterial and antiviral components include the following raw materials in parts: 15 parts Coptis chinensis, 10 parts Phellodendron chinense, 8 parts Isatis indigotica, and 8 parts Lonicera japonica. The insect repellent component comprises the following ingredients in parts: 15 parts Sophora flavescens, 10 parts Neem, 8 parts Rhizoma Ctenoptera, and 5 parts Artemisia argyi. The root-promoting immune-inducing component comprises the following raw materials in parts: 10 parts Astragalus membranaceus, 5 parts Glycyrrhiza uralensis, and 4 parts Humic acid; The additives include the following raw materials in parts: 2 parts saponin penetrant and 2 parts polysaccharide stabilizer.

4. The preparation method of a traditional Chinese medicine compound preparation for green control of areca nut yellowing disease according to claim 1, characterized in that, Includes the following steps: Step 1: Preparation of raw Chinese medicinal materials and processed Chinese medicinal slices: Prepare raw Chinese medicinal materials and processed Chinese medicinal slices according to their components; Step 2, Mixing: Place the prepared raw Chinese medicine and processed medicinal materials into a mixer for mixing; Step 3, Grinding: Place the mixed raw Chinese medicine and processed Chinese medicine into a grinder and grind them separately, then sieve them through a 60-80 mesh screen; Step 4: Boil with water: Place the crushed and sieved raw materials into a boiling device, add 8 to 12 times the amount of water, and boil. Step 5: Extraction of traditional Chinese medicine compound preparations: Place the raw materials after decoction into the extraction equipment and extract 3 to 5 times at a temperature of 90 to 100℃, each time for 1.5 to 2 hours; Step 6, Cooling: Transfer the extracted liquid to a stainless steel cooling tank and allow it to cool naturally or with circulating water to room temperature. Stir every 15 minutes during the cooling process. After cooling, perform coarse filtration through a 300-mesh filter cloth to remove the residue and insoluble impurities, and obtain the preliminary filtrate. Step 7, Negative pressure filtration: Transfer the preliminary filtrate to a centrifuge to remove fine suspended particles and obtain a clear filtrate; Step 8: Concentrate the original Chinese medicine liquid: Place the obtained clear filtrate in a vacuum concentrator and concentrate it under reduced pressure until the solid content is 5% to 8%; Step 9: Compound preparation of traditional Chinese medicine: Add adjuvants, stir to dissolve, homogenize, sterilize, and fill.

5. The preparation and application method of a traditional Chinese medicine compound preparation for green control of areca nut yellowing disease according to claim 4, characterized in that, The mixer is a double-helix conical mixer with a mixing speed of 20-30 r / min and a mixing time of 15-25 minutes.

6. The preparation method of a traditional Chinese medicine compound preparation for green control of areca nut yellowing disease according to claim 4, characterized in that, The crusher is a hammer mill, and the feeding speed is controlled at 100-150 kg / h during crushing. After crushing, the material passes through a 70-mesh sieve.

7. The preparation method of a traditional Chinese medicine compound preparation for green control of areca nut yellowing disease according to claim 4, characterized in that, The cooking equipment uses a stainless steel jacketed kettle, which maintains a gentle boil during the cooking process and is stirred once every 30 minutes.

8. The preparation method of a traditional Chinese medicine compound preparation for green control of areca nut yellowing disease according to claim 4, characterized in that, The extraction equipment uses a multi-functional extraction tank. During the extraction process, the pressure inside the tank is maintained at 0.05–0.1 MPa, and the circulation flow rate of the extract is 2–3 m / s. 3 / h.

9. The preparation method of a traditional Chinese medicine compound preparation for green control of areca nut yellowing disease according to claim 4, characterized in that, The centrifuge was used to centrifuge at 3000 r / min for 15 minutes.

10. A method for green control of areca nut yellowing disease using a traditional Chinese medicine compound preparation, comprising the traditional Chinese medicine compound preparation according to any one of claims 1-9, characterized in that, include: Disease prevention period (new shoots / flowering period): Dilute the Chinese herbal compound preparation 300-500 times and spray on the leaves, once every 10-15 days, for 2-3 consecutive times; In the early stage of the disease: dilute 200-300 times for spraying and 500-800 times for root irrigation, 5-10 kg per plant per irrigation, once every 10 days, for 2-3 consecutive applications; Rejuvenation and restoration period: Combine with drip irrigation / fertigation of organic fertilizer to improve the root zone and enhance tree vigor.

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