An engineered butyri vibrio pathogenicum and a fermentation process and application thereof
By replacing the promoter of Budapest pathogenic bacillus and optimizing the fermentation process, a strain that produces high-yield flebotryptamine derivatives was constructed, solving the problem of low flebotryptamine yield in existing strains and achieving a significant increase in yield.
CN122235033APending Publication Date: 2026-06-19INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-03-25
- Publication Date
- 2026-06-19
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Figure CN122235033A_ABST
Abstract
This invention discloses an engineered strain of *Pathogenic Bacillus budapestans*, its fermentation process, and its applications. Starting with *Pathogenic Bacillus budapestans* XBD101, this invention obtains *Pathogenic Bacillus budapestans* strain FH1 by sequentially replacing the original fclC promoter with P1; strain FH2 by replacing the original gene2204 promoter of strain FH1 with P1; strain FH3 by replacing the original fclI promoter of strain FH2 with P19; and strain FH4 by replacing the original fclK promoter of strain FH3 with P6. The yield of the fcl-8 peptide derivative from strains FH1 through FH4 is significantly increased sequentially. Further fermentation optimization further increases the yield to 943.39 mg / L, a 13.56-fold increase compared to XBD101, which significantly promotes the industrialization of this type of natural product.
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Citation Information
Patent Citations
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