Methods and compositions for donor DNA molecules
By using circular single-stranded DNA (cssDNA) donor molecules to form a double-stranded DNA region with substrate complementary oligonucleotides (SCO), and then combining with endogenous DNA polymerase for second-strand synthesis, the risks of immune sensing and integration of dsDNA donor molecules in the cell nucleus are resolved, enabling safe and efficient genome insertion.
CN122374459APending Publication Date: 2026-07-10THE GENERAL HOSPITAL CORP
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- THE GENERAL HOSPITAL CORP
- Filing Date
- 2024-10-11
- Publication Date
- 2026-07-10
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Abstract
We describe compositions of DNA donor molecules compatible with various gene insertion methods, offering key advantages in delivery, nuclear localization, and immune evasion. The modified DNA donor molecules are predominantly single-stranded but include short regions of double-stranded DNA to reconstruct enzyme binding sites, and can be combined with other methods to enhance donor recruitment to the cell nucleus.
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Citation Information
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